A high-throughput microfluidic microphysiological system (PREDICT-96) to recapitulate hepatocyte function in dynamic, re-circulating flow conditions

Lab on a Chip ◽  
2019 ◽  
Vol 19 (9) ◽  
pp. 1556-1566 ◽  
Author(s):  
Kelly Tan ◽  
Philip Keegan ◽  
Miles Rogers ◽  
Mingjian Lu ◽  
James R. Gosset ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Flow Conditions ◽  
Culture Systems ◽  
Cell Culture Systems ◽  
Microphysiological Systems ◽  
External Cues ◽  
Organ Specific ◽  
Dynamic Cell

Microphysiological systems (MPSs) are dynamic cell culture systems that provide micro-environmental and external cues to support physiologically relevant, organ-specific functions.

scholarly journals Spectroscopic methods and their applicability for high-throughput characterization of mammalian cell cultures in automated cell culture systems

2016 ◽  
Vol 16 (5) ◽  
pp. 405-416 ◽  
Author(s):  
Carsten Musmann ◽  
Klaus Joeris ◽  
Sven Markert ◽  
Dörte Solle ◽  
Thomas Scheper
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Cell Cultures ◽  
High Throughput ◽  
Spectroscopic Methods ◽  
Culture Systems ◽  
Mammalian Cell Cultures ◽  
Cell Culture Systems

scholarly journals The Use of 3-D Cultures for High-Throughput Screening: The Multicellular Spheroid Model

2004 ◽  
Vol 9 (4) ◽  
pp. 273-285 ◽  
Author(s):  
Leoni A. Kunz-Schughart ◽  
James P. Freyer ◽  
Ferdinand Hofstaedter ◽  
Reinhard Ebner
Keyword(s):  
Cell Culture ◽  
Drug Development ◽  
High Throughput ◽  
High Throughput Screening ◽  
In Vivo Models ◽  
Culture Systems ◽  
Test Systems ◽  
Cell Culture Systems ◽  
D Cell

Over the past few years, establishment and adaptation of cell-based assays for drug development and testing has become an important topic in high-throughput screening (HTS). Most new assays are designed to rapidly detect specific cellular effects reflecting action at various targets. However, although more complex than cell-free biochemical test systems, HTS assays using monolayer or suspension cultures still reflect a highly artificial cellular environment and may thus have limited predictive value for the clinical efficacy of a compound. Today’s strategies for drug discovery and development, be they hypothesis free or mechanism based, require facile, HTS-amenable test systems that mimic the human tissue environment with increasing accuracy in order to optimize preclinical and preanimal selection of the most active molecules from a large pool of potential effectors, for example, against solid tumors. Indeed, it is recognized that 3-dimensional cell culture systems better reflect the in vivo behavior of most cell types. However, these 3-D test systems have not yet been incorporated into mainstream drug development operations. This article addresses the relevance and potential of 3-D in vitro systems for drug development, with a focus on screening for novel antitumor drugs. Examples of 3-D cell models used in cancer research are given, and the advantages and limitations of these systems of intermediate complexity are discussed in comparison with both 2-D culture and in vivo models. The most commonly used 3-D cell culture systems, multicellular spheroids, are emphasized due to their advantages and potential for rapid development as HTS systems. Thus, multicellular tumor spheroids are an ideal basis for the next step in creating HTS assays, which are predictive of in vivo antitumor efficacy.

scholarly journals Electrospun hydrogels for dynamic culture systems: advantages, progress, and opportunities

2021 ◽  
Author(s):  
M. Gregory Grewal ◽  
Christopher B. Highley
Keyword(s):  
Cell Culture ◽  
Culture Systems ◽  
Dynamic Culture ◽  
Cell Culture Systems ◽  
Dynamic Cell ◽  
And Control ◽  
Soft Biomaterials

Hydrogel nanofibers build on established soft biomaterials to enable design and control of unique, dynamic cell culture systems.

scholarly journals Smart Cell Culture Systems: Integration of Sensors and Actuators into Microphysiological Systems

2018 ◽  
Vol 13 (7) ◽  
pp. 1767-1784 ◽  
Author(s):  
Mario M. Modena ◽  
Ketki Chawla ◽  
Patrick M. Misun ◽  
Andreas Hierlemann
Keyword(s):  
Cell Culture ◽  
Systems Integration ◽  
Sensors And Actuators ◽  
Culture Systems ◽  
Cell Culture Systems ◽  
Microphysiological Systems

Recent Progress in Prediction Systems for Drug-induced Liver Injury Using in vitro Cell Culture

2020 ◽  
Vol 14 ◽  
Author(s):  
Shogo Ozawa ◽  
Toshitaka Miura ◽  
Jun Terashima ◽  
Wataru Habano ◽  
Seiichi Ishida
Keyword(s):  
Cell Culture ◽  
Recent Progress ◽  
Inflammatory Cells ◽  
Protein Adducts ◽  
In Vitro Assays ◽  
Drug Induced ◽  
Drug Induced Liver Injury ◽  
Culture Systems ◽  
Cell Culture Systems

Background: In order to avoid drug-induced liver injury (DILI), in vitro assays, which enable the assessment of both metabolic activation and immune reaction processes that ultimately result in DILI, are needed. Objective: In this study, the recent progress in the application of in vitro assays using cell culture systems is reviewed for potential DILI-causing drugs/xenobiotics and a mechanistic study on DILI, as well as for the limitations of in vitro cell culture systems for DILI research. Methods: Information related to DILI was collected through a literature search of the PubMed database. Results: The initial biological event for the onset of DILI is the formation of cellular protein adducts after drugs have been metabolically activated by drug metabolizing enzymes. The damaged peptides derived from protein adducts lead to the activation of CD4+ helper T lymphocytes and recognition by CD8+ cytotoxic T lymphocytes, which destroy hepatocytes through immunological reactions. Because DILI is a major cause of drug attrition and drug withdrawal, numerous in vitro systems consisting of hepatocytes and immune/inflammatory cells, or spheroids of human primary hepatocytes containing non-parenchymal cells have been developed. These cellular-based systems have identified DILIinducing drugs with approximately 50% sensitivity and 90% specificity. Conclusion: Different co-culture systems consisting of human hepatocyte-derived cells and other immune/inflammatory cells have enabled the identification of DILI-causing drugs and of the actual mechanisms of action.

scholarly journals Mammary gland 3D cell culture systems in farm animals

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Laurence Finot ◽  
Eric Chanat ◽  
Frederic Dessauge
Keyword(s):  
Cell Culture ◽  
Mammary Gland ◽  
Bacterial Infections ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Farm Animals ◽  
Culture Systems ◽  
Cell Culture Systems

AbstractIn vivo study of tissue or organ biology in mammals is very complex and progress is slowed by poor accessibility of samples and ethical concerns. Fortunately, however, advances in stem cell identification and culture have made it possible to derive in vitro 3D “tissues” called organoids, these three-dimensional structures partly or fully mimicking the in vivo functioning of organs. The mammary gland produces milk, the source of nutrition for newborn mammals. Milk is synthesized and secreted by the differentiated polarized mammary epithelial cells of the gland. Reconstructing in vitro a mammary-like structure mimicking the functional tissue represents a major challenge in mammary gland biology, especially for farm animals for which specific agronomic questions arise. This would greatly facilitate the study of mammary gland development, milk secretion processes and pathological effects of viral or bacterial infections at the cellular level, all with the objective of improving milk production at the animal level. With this aim, various 3D cell culture models have been developed such as mammospheres and, more recently, efforts to develop organoids in vitro have been considerable. Researchers are now starting to draw inspiration from other fields, such as bioengineering, to generate organoids that would be more physiologically relevant. In this chapter, we will discuss 3D cell culture systems as organoids and their relevance for agronomic research.

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