Achieving the ratiometric imaging of steroid sulfatase in living cells and tissues with a two-photon fluorescent probe

2020 ◽  
Vol 56 (9) ◽  
pp. 1349-1352 ◽  
Author(s):  
Wei Li ◽  
Shulu Yin ◽  
Xiangyang Gong ◽  
Wang Xu ◽  
Ronghua Yang ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Fluorescence Assay ◽  
Steroid Sulfatase ◽  
Ratiometric Fluorescence ◽  
Two Photon ◽  
Ratiometric Imaging ◽  
Endogenous Steroid

A novel two-photon ratiometric fluorescence assay was proposed for monitoring endogenous steroid sulfatase (STS) activity, which achieved the ratiometric imaging of STS activity in the endoplasmic reticulum of living cells and tissues.

A two-photon endoplasmic reticulum-targeting fluorescent probe for the imaging of pH in living cells and zebrafish

2018 ◽  
Vol 10 (47) ◽  
pp. 5702-5706 ◽  
Author(s):  
Nan Zhang ◽  
Baoli Dong ◽  
Xiuqi Kong ◽  
Wenhui Song ◽  
Weiying Lin
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Ph Changes ◽  
Two Photon ◽  
Endoplasmic Reticulum Targeting

The pH changes of the endoplasmic reticulum (ER) are closely related to many diseases.

scholarly journals Ratiometric fluorescence imaging of Golgi H2O2 reveals a correlation between Golgi oxidative stress and hypertension

2019 ◽  
Vol 10 (47) ◽  
pp. 10876-10880 ◽  
Author(s):  
Hui Wang ◽  
Zixu He ◽  
Yuyun Yang ◽  
Jiao Zhang ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Living Systems ◽  
Ratiometric Fluorescence ◽  
Two Photon ◽  
Group A ◽  
Ratiometric Imaging

Based on a novel Golgi-targeting phenylsulfonamide group, a two-photon (TP) fluorescent probe, Np-Golgi, was developed for in situ H2O2 ratiometric imaging in living systems.

Development of an endoplasmic reticulum-targeting fluorescent probe for the two-photon imaging of hypochlorous acid (HClO) in living cells

2019 ◽  
Vol 11 (35) ◽  
pp. 4450-4455 ◽  
Author(s):  
Wenhui Song ◽  
Baoli Dong ◽  
Yaru Lu ◽  
Xiuqi Kong ◽  
Abdul Hadi Mehmood ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Oxygen Species ◽  
Cell Dysfunction ◽  
Two Photon ◽  
Endoplasmic Reticulum Targeting

As an important reactive oxygen species, hypochlorous acid (HClO) could irreversibly destroy Ca2+-ATPase to inhibit Ca2+ transport in the endoplasmic reticulum (ER), which may eventually lead to cell dysfunction and cell death.

Molecular Engineering of a TBET-Based Two-Photon Fluorescent Probe for Ratiometric Imaging of Living Cells and Tissues

2014 ◽  
Vol 136 (28) ◽  
pp. 9838-9841 ◽  
Author(s):  
Liyi Zhou ◽  
Xiaobing Zhang ◽  
Qianqian Wang ◽  
Yifan Lv ◽  
Guojiang Mao ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Molecular Engineering ◽  
Two Photon ◽  
Ratiometric Imaging

A facile two-photon fluorescent probe: an endoplasmic reticulum tracker monitoring ER stress and vesicular transport to lysosomes

2019 ◽  
Vol 55 (3) ◽  
pp. 294-297 ◽  
Author(s):  
Pratibha Kumari ◽  
Sanjay K. Verma ◽  
Shaikh M. Mobin
Keyword(s):  
Endoplasmic Reticulum ◽  
Er Stress ◽  
Fluorescent Probe ◽  
Vesicular Transport ◽  
Living Cells ◽  
Vesicle Transport ◽  
Live Cells ◽  
Tumor Spheroids ◽  
Dynamic Changes ◽  
Two Photon

The two-photon fluorescent organic probe ERLp selectively labels the endoplasmic reticulum in live cells and tumor spheroids. ERLp can also monitor ER dynamic changes during ER stress and vesicle transport from the ER to the lysosome in living cells.

A smart mitochondria-targeting TP-NIR fluorescent probe for selective and sensitive sensing H2S in living cells and mice

2021 ◽  
Author(s):  
Qiaomei Yang ◽  
Liyi Zhou ◽  
Longpeng Peng ◽  
Gangqiang Yuan ◽  
Haiyuan Ding ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells ◽  
Signal Molecules ◽  
Two Photon ◽  
Mitochondria Targeting

Hydrogen sulfide (H2S) is one of the important gaseous signal molecules and plays key roles in various biologically crucial processes. In this work, we report a novel two-photon near-infrared (TP-NIR)...

Ratiometric fluorescence probe for two-photon bioimaging of Cr3+ in living cells

2014 ◽  
Vol 55 (30) ◽  
pp. 4075-4077 ◽  
Author(s):  
Zhiguo Zhou ◽  
Ying Li ◽  
Yongquan Wu
Keyword(s):  
Fluorescence Probe ◽  
Living Cells ◽  
Ratiometric Fluorescence ◽  
Two Photon

A mitochondria-targeted two-photon fluorescent probe for highly selective and rapid detection of hypochlorite and its bio-imaging in living cells

2016 ◽  
Vol 222 ◽  
pp. 483-491 ◽  
Author(s):  
Daoxue Li ◽  
Yan Feng ◽  
Jizhi Lin ◽  
Man Chen ◽  
Shuxin Wang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Rapid Detection ◽  
Living Cells ◽  
Two Photon ◽  
Bio Imaging
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