scholarly journals Parallelizable microfluidic dropmakers with multilayer geometry for the generation of double emulsions

Lab on a Chip ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Saraf Nawar ◽  
Joshuah K. Stolaroff ◽  
Congwang Ye ◽  
Huayin Wu ◽  
Du Thai Nguyen ◽  
...  
Keyword(s):  
Double Emulsion ◽  
Microfluidic Devices ◽  
Surface Wettability ◽  
Double Emulsions ◽  
Channel Surface ◽  
Emulsion Drops ◽  
Scalable Production

We present a multilayer dropmaker geometry that enables the modular fabrication of microfluidic devices containing precisely patterned channel surface wettability. The platform is used for the scalable production of uniform double emulsion drops.

scholarly journals Scalable production of double emulsion drops with thin shells

Lab on a Chip ◽  
2018 ◽  
Vol 18 (13) ◽  
pp. 1936-1942 ◽  
Author(s):  
A. Vian ◽  
B. Reuse ◽  
E. Amstad
Keyword(s):  
High Throughput ◽  
Shell Thickness ◽  
Double Emulsion ◽  
Thin Shells ◽  
Double Emulsions ◽  
Emulsion Drops ◽  
Scalable Production

The microfluidic aspiration device reduces the shell thickness of double emulsions down to 240 nm at a high throughput.

Composite Microgels Created by Complexation between Polyvinyl Alcohol and Graphene Oxide in Compressed Double‐Emulsion Drops

Small ◽  
2019 ◽  
Vol 16 (9) ◽  
pp. 1903812 ◽  
Author(s):  
Ye Hun Choi ◽  
Sang Seok Lee ◽  
Dong‐Myeong Lee ◽  
Hyeon Su Jeong ◽  
Shin‐Hyun Kim
Keyword(s):  
Graphene Oxide ◽  
Polyvinyl Alcohol ◽  
Double Emulsion ◽  
Emulsion Drops

Double-emulsion drops with ultra-thin shells for capsule templates

Lab on a Chip ◽  
2011 ◽  
Vol 11 (18) ◽  
pp. 3162-3166 ◽  
Author(s):  
Shin-Hyun Kim ◽  
Jin Woong Kim ◽  
Jun-Cheol Cho ◽  
David A. Weitz
Keyword(s):  
Double Emulsion ◽  
Thin Shells ◽  
Emulsion Drops

scholarly journals Synchronized Reagent Delivery in Double Emulsions for Triggering Chemical Reactions and Gene Expression

2021 ◽  
Author(s):  
Ariane Stucki ◽  
Petra Jusková ◽  
Nicola Nuti ◽  
Steven Schmitt ◽  
Petra Dittrich
Keyword(s):  
Gene Expression ◽  
High Throughput ◽  
High Throughput Screening ◽  
Double Emulsion ◽  
Lipid Vesicles ◽  
Enzyme Engineering ◽  
Droplet Microfluidics ◽  
Enzymatic Reactions ◽  
High Throughput Analysis ◽  
Double Emulsions

Microfluidic methods to form single emulsion and double emulsion (DE) droplets have greatly enhanced the toolbox for high throughput screening for cell or enzyme engineering and drug discovery. However, remaining challenges in the supply of reagents into these enclosed nanoliter compartments limit the applicability of droplet microfluidics. Here, we introduce a strategy for on-demand delivery of reactants in DEs. We use lipid vesicles as transport carriers, which are co-encapsulated in double emulsions and release their cargo upon addition of an external trigger, here the anionic surfactant SDS. The reagent present inside the lipid vesicles stays isolated from the remaining content of the DE vessel until SDS enters the DE lumen and solubilizes the lipid bilayer. We demonstrate the versatility of the method with two critical applications, chosen as representative assays for high throughput screening. First, we trigger enzymatic reactions after releasing a reactant and second, we encapsulate bacteria and induce gene expression at a delayed time. The presented technique is compatible with the high throughput analysis of individual DE droplets using conventional flow cytometry as well as with microfluidic time-resolved studies. The possibility of delaying and controlling reagent delivery in current high throughput compartmentalization systems will significantly extend their range of applications e.g. for directed evolution, and further improve their compatibility with biological systems.

scholarly journals Effect of Selected Stabilizers and Processing Aids on the Stability of a Double Emulsion Encapsulating Bitter Gourd Extract

2021 ◽  
Author(s):  
Urmila Choudhary ◽  
Latha Sabikhi
Keyword(s):  
Double Emulsion ◽  
Gum Arabic ◽  
Sodium Caseinate ◽  
Bitter Gourd ◽  
Whey Protein Concentrate ◽  
Physical Parameters ◽  
Sedimentation Stability ◽  
Double Emulsions ◽  
The Stability ◽  
Emulsion Matrix

Effect of three variables in differing concentrations [NaCl (3-5%), polyglycerol polyricinoleate (PGPR) (2-4%) and dairy protein-polysaccharide complexes (Whey protein concentrate(WPC-80)-gum Arabic(GA) and sodium caseinate(SC)-gum Arabic in 1:2 ratio)] on the stability of W1/O/W2 emulsion matrix that was used to encapsulate bitter gourd extract was evaluated. The double emulsion matrix was characterized by apparent viscosity, zeta potential, turbidity and sedimentation stability by visual appearance. The physical parameters of the double emulsion matrix were very highly significantly (p < 0.001) affected by all variables such as the concentration of salt, PGPR and complex (WPC-GA and SC-P) as well as their interactions. The double emulsions prepared with WPC-GA became unstable immediately after preparation or after one day of preparation. SC-GA stabilized double emulsions were found more stable than WPC-GA stabilized emulsions. A double emulsion containing 5% NaCl, 2% PGPR and 16.5% SC-GA were found most stable (10 days at 37°C) in comparison to other combinations used.

scholarly journals Synchronized Reagent Delivery in Double Emulsions for Triggering Chemical Reactions and Gene Expression

2021 ◽  
Author(s):  
Ariane Stucki ◽  
Petra Jusková ◽  
Nicola Nuti ◽  
Steven Schmitt ◽  
Petra Dittrich
Keyword(s):  
Gene Expression ◽  
High Throughput ◽  
High Throughput Screening ◽  
Double Emulsion ◽  
Lipid Vesicles ◽  
Enzyme Engineering ◽  
Droplet Microfluidics ◽  
Enzymatic Reactions ◽  
High Throughput Analysis ◽  
Double Emulsions

Microfluidic methods to form single emulsion and double emulsion (DE) droplets have greatly enhanced the toolbox for high throughput screening for cell or enzyme engineering and drug discovery. However, remaining challenges in the supply of reagents into these enclosed nanoliter compartments limit the applicability of droplet microfluidics. Here, we introduce a strategy for on-demand delivery of reactants in DEs. We use lipid vesicles as transport carriers, which are co-encapsulated in double emulsions and release their cargo upon addition of an external trigger, here the anionic surfactant SDS. The reagent present inside the lipid vesicles stays isolated from the remaining content of the DE vessel until SDS enters the DE lumen and solubilizes the lipid bilayer. We demonstrate the versatility of the method with two critical applications, chosen as representative assays for high throughput screening. First, we trigger enzymatic reactions after releasing a reactant and second, we encapsulate bacteria and induce gene expression at a delayed time. The presented technique is compatible with the high throughput analysis of individual DE droplets using conventional flow cytometry as well as with microfluidic time-resolved studies. The possibility of delaying and controlling reagent delivery in current high throughput compartmentalization systems will significantly extend their range of applications e.g. for directed evolution, and further improve their compatibility with biological systems.

scholarly journals Addition of Trans-Resveratrol-Loaded Highly Concentrated Double Emulsion to Yoghurts: Effect on Physicochemical Properties

2021 ◽  
Vol 23 (1) ◽  
pp. 85
Author(s):  
Rocío Díaz-Ruiz ◽  
Amanda Laca ◽  
Marta Sánchez ◽  
Manuel Ramón Fernández ◽  
María Matos ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Droplet Size ◽  
Storage Time ◽  
Control Sample ◽  
Double Emulsion ◽  
Colour Variation ◽  
Double Emulsions ◽  
Appreciable Increase ◽  
Different Types ◽  
The Stability

Trans-resveratrol (RSV) needs to be encapsulated to maintain its beneficial properties on the human body. This is due to its extreme photosensitivity, short biological half-life, and easy oxidation. In this study, the use of double emulsions for RSV encapsulation and their further application on functional yoghurts was studied. Different types of yoghurts were prepared: with and without RSV and with two types of volumetric emulsion formulations (20/80 and 30/70). In order to study the influence of the addition of double emulsions to the physical properties of the prepared yoghurts, they were characterised fresh and after a month under storage at 4 °C, in terms of droplet size, morphology, stability, rheology, texturometry, colorimetry, and antioxidant capacity. Results obtained showed that the presence of emulsion in the yoghurts produced a generalised decrease in the predominant droplet size (from 48 µm to 15–25 µm) and an increase in the stability. Additionally, a predominantly elastic character was observed. The firmness values obtained were very similar for all the yoghurts analysed and did not suffer important modifications with time. A slight colour variation was observed with storage time in the control sample, whereas a more notable variation in the case of emulsion yoghurts was observed. An appreciable increase of the antioxidant capacity of the final functional yoghurt (100 g) was observed when it contained 5–8 mg of RSV. Encapsulated RSV added to yoghurts presented a larger protection against RSV oxidation compared with free RSV, presenting a larger antioxidant inhibition after one month of storage. Moreover, the antioxidant capacity of yoghurts with encapsulated RSV was not affected under storage, since slight reductions (3%) were registered after one month of storage at 4 °C.

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