scholarly journals Dynamic surface properties of PEG-coated CuS nanoparticles alter their interaction with cells as revealed by surface-enhanced infrared spectroscopy

2019 ◽  
Vol 1 (11) ◽  
pp. 4268-4276
Author(s):  
Fengjuan Cao ◽  
Lie Wu ◽  
Xiaofei Zhang ◽  
Shanshan Li ◽  
Chao Wang ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Real Time ◽  
Surface Properties ◽  
Cell Interaction ◽  
Label Free ◽  
Dynamic Changes ◽  
Dynamic Surface ◽  
Surface Enhanced ◽  
First Time

How the dynamic changes of the surface properties of nanoparticles affect the nano-cell interaction was revealed for the first time, by in situ real-time label-free SEIRA spectroscopy at the nanoscale.

Probing the Dynamic Self-Assembly Behaviour of Photoswitchable Wormlike Micelles in Real Time

2018 ◽  
Author(s):  
Elaine A. Kelly ◽  
Judith E. Houston ◽  
Rachel Evans
Keyword(s):  
Real Time ◽  
Absorption Spectroscopy ◽  
Self Assembly ◽  
Uv Light ◽  
Wormlike Micelles ◽  
Tetraethylene Glycol ◽  
Light Illumination ◽  
First Time ◽  
Dependent Processes

Understanding the dynamic self-assembly behaviour of azobenzene photosurfactants (AzoPS) is crucial to advance their use in controlled release applications such as<i></i>drug delivery and micellar catalysis. Currently, their behaviour in the equilibrium <i>cis-</i>and <i>trans</i>-photostationary states is more widely understood than during the photoisomerisation process itself. Here, we investigate the time-dependent self-assembly of the different photoisomers of a model neutral AzoPS, <a>tetraethylene glycol mono(4′,4-octyloxy,octyl-azobenzene) </a>(C<sub>8</sub>AzoOC<sub>8</sub>E<sub>4</sub>) using small-angle neutron scattering (SANS). We show that the incorporation of <i>in-situ</i>UV-Vis absorption spectroscopy with SANS allows the scattering profile, and hence micelle shape, to be correlated with the extent of photoisomerisation in real-time. It was observed that C<sub>8</sub>AzoOC<sub>8</sub>E<sub>4</sub>could switch between wormlike micelles (<i>trans</i>native state) and fractal aggregates (under UV light), with changes in the self-assembled structure arising concurrently with changes in the absorption spectrum. Wormlike micelles could be recovered within 60 seconds of blue light illumination. To the best of our knowledge, this is the first time the degree of AzoPS photoisomerisation has been tracked <i>in</i><i>-situ</i>through combined UV-Vis absorption spectroscopy-SANS measurements. This technique could be widely used to gain mechanistic and kinetic insights into light-dependent processes that are reliant on self-assembly.

Label-free and real time monitoring of adipocyte differentiation by surface infrared spectroscopy

2013 ◽  
Vol 176 ◽  
pp. 1176-1182 ◽  
Author(s):  
Yuki Aonuma ◽  
Yasuhiko Kondo ◽  
Ayumi Hirano-Iwata ◽  
Atena Nishikawa ◽  
Yasuo Shinohara ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Real Time ◽  
Adipocyte Differentiation ◽  
Label Free ◽  
Real Time Monitoring

Tracking the Formation and Degradation of Fatty-Acid-Accumulated Mitochondria Using Label-Free Chemical Imaging

2020 ◽  
Author(s):  
Chi Zhang ◽  
Stephen Boppart
Keyword(s):  
Fatty Acid ◽  
Structural Features ◽  
Chemical Imaging ◽  
Live Cells ◽  
Label Free ◽  
Dynamic Changes ◽  
Regulatory Processes ◽  
Transport Chain ◽  
Anti Stokes ◽  
First Time

Abstract The mitochondrion is one of the key organelles for maintaining cellular homeostasis. External environmental stimuli and internal regulatory processes alter the metabolism and functions of mitochondria. To understand these activities of mitochondria, it is critical to probe the key metabolic molecules inside these organelles. In this study, we used label-free chemical imaging modalities including coherent anti-Stokes Raman scattering and multiphoton-excited autofluorescence to study the mitochondrial activities in living cancer cells. We found that hypothermia exposure tends to induce fatty-acid (FA) accumulation in some mitochondria of MIAPaCa-2 cells. Autofluorescence images show that the FA-accumulated mitochondria also have abnormal NADH and FAD metabolism, likely induced by the dysfunction of the electron transport chain. We also found that when the cells were re-warmed to physiological temperature after a period of hypothermia, the FA-accumulated mitochondria changed their structural features, likely caused by the mitophagy process. To the best of our knowledge, this is the first time that FA accumulation in mitochondria was observed in live cells. Our research also demonstrates that multimodal label-free chemical imaging is an attractive tool to discover abnormal functions of mitochondria at the single-organelle level and can be used to quantify the dynamic changes of this organelle under perturbative conditions.

In Situ Real-Time Studies of Heterogeneous Catalytic Mechanisms at Ambient Pressures As Probed by Surface-Enhanced Raman and Mass Spectroscopies

1998 ◽  
Vol 37 (6) ◽  
pp. 2307-2315 ◽  
Author(s):  
Christopher T. Williams ◽  
Ho Yeung H. Chan ◽  
Anish A. Tolia ◽  
Michael J. Weaver ◽  
Christos G. Takoudis
Keyword(s):  
Real Time ◽  
Heterogeneous Catalytic ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Catalytic Mechanisms

Directly monitoring the active sites of charge transfer in heterocycles in situ and in real time

2019 ◽  
Vol 55 (4) ◽  
pp. 541-544 ◽  
Author(s):  
Shuji Ye ◽  
Junjun Tan ◽  
Kangzhen Tian ◽  
Chuanzhao Li ◽  
Jiahui Zhang ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Real Time ◽  
Vibrational Spectroscopy ◽  
Active Sites ◽  
Electronic States ◽  
Sum Frequency Generation ◽  
Vibrational Modes ◽  
Label Free ◽  
Sum Frequency

Coherent degenerate infrared-infrared-visible sum frequency generation vibrational spectroscopy provides a powerful label-free probe for identifying the vibrational modes that are coupled through the electronic states in situ and in real time.

A method for real time detection of reaction endpoints using a moving window t-test of in situ time course data

2020 ◽  
Vol 5 (9) ◽  
pp. 1642-1646
Author(s):  
Elyse N. T. DiMaso ◽  
Robert W. Bondi ◽  
John Guo ◽  
Alexander G. O'Brien
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Real Time ◽  
Time Course ◽  
Test Method ◽  
Moving Window ◽  
Mid Infrared ◽  
Mid Infrared Spectroscopy ◽  
Time Course Data

In-line Fourier transform mid-infrared spectroscopy (FTIR) in combination with a moving window t-test method was used to determine the endpoint of a reaction with a highly inconsistent rate.

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