scholarly journals PVA and CS cross-linking combined with in situ chimeric SiO2 nanoparticle adhesion to enhance the hydrophilicity and antibacterial properties of PTFE flat membranes

RSC Advances ◽  
2019 ◽  
Vol 9 (33) ◽  
pp. 19205-19216 ◽  
Author(s):  
Chengcai Li ◽  
Hang Zhang ◽  
Feng Wang ◽  
Hailin Zhu ◽  
Yuhai Guo ◽  
...  
Keyword(s):  
Preparation Method ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Cross Linking ◽  
Sio2 Nanoparticle ◽  
Simple Preparation ◽  
Cross Linker ◽  
Flat Membranes

Herein, a new hydrophilic and antibacterial PTFE flat MF membrane was fabricated via a low-cost and simple preparation method in which CS was crosslinked with PVA using ECH as a cross-linker followed by in situ chimeric SiO2 nanoparticle adhesion.

To Eliminate Curtain Effect of FIB TEM Samples by a Combination of Sample Dicing and Backside Milling

2014 ◽  
Author(s):  
Jian-Shing Luo ◽  
Hsiu Ting Lee
Keyword(s):  
Sample Preparation ◽  
Focused Ion Beam ◽  
Preparation Method ◽  
Low Cost ◽  
Ion Beam ◽  
Sample Preparation Method ◽  
Site Specific ◽  
Dual Beam ◽  
Transmission Electron

Abstract Several methods are used to invert samples 180 deg in a dual beam focused ion beam (FIB) system for backside milling by a specific in-situ lift out system or stages. However, most of those methods occupied too much time on FIB systems or requires a specific in-situ lift out system. This paper provides a novel transmission electron microscopy (TEM) sample preparation method to eliminate the curtain effect completely by a combination of backside milling and sample dicing with low cost and less FIB time. The procedures of the TEM pre-thinned sample preparation method using a combination of sample dicing and backside milling are described step by step. From the analysis results, the method has applied successfully to eliminate the curtain effect of dual beam FIB TEM samples for both random and site specific addresses.

Dual cross-linking ribonucleoprotein immunoprecipitation assay

2014 ◽  
Vol 92 (4) ◽  
pp. 317-319 ◽  
Author(s):  
Dilshad H. Khan ◽  
James R. Davie
Keyword(s):  
Uv Irradiation ◽  
Protein Interactions ◽  
Uv Light ◽  
Target Identification ◽  
Cross Linking ◽  
Immunoprecipitation Assay ◽  
Cross Linker ◽  
Subsequent Target ◽  
Protein Cross Linking

Ribonucleoprotein immunoprecipitation (RIP) is an antibody-based method to detect RNA–protein interactions in situ. In the assay, UV cross-linking is commonly used to preserve RNA–protein interactions for subsequent target identification. UV light is a zero-length cross linker and thus identifies proteins directly bound to RNAs. Here, we describe a dual cross-linking RIP method that involves sequential protein–protein cross-linking step with a protein–protein cross-linker, followed by protein–RNA fixation by UV irradiation. In this way, proteins that indirectly bound to RNA can be analyzed.

In Situ Cross-linking Polymerization-Induced Self-Assembly: Not Only Generates Cross-linked Structures But Also Promotes Morphology Transition by the Cross-linker

2021 ◽  
Author(s):  
Jamshid Kadirkhanov ◽  
Cheng-Lin Yang ◽  
Zi-Xuan Chang ◽  
Ren-Man Zhu ◽  
Cai-Yuan Pan ◽  
...  
Keyword(s):  
Self Assembly ◽  
Cross Linking ◽  
Morphology Transition ◽  
Convenient Approach ◽  
Cross Linker ◽  
The Cross

Compared with the post-polymerization cross-linking strategy, in situ cross-linking by divinyl comonomers in polymerization-induced self-assembly (PISA) is a more straightforward and convenient approach to produce structurally stabilized nano-objects. However, cross-linking...

scholarly journals Cranberry Juice Extract Rapidly Protects Demineralized Dentin against Digestion and Inhibits Its Gelatinolytic Activity

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3637
Author(s):  
Yong Wang ◽  
Austin Green ◽  
Xiaomei Yao ◽  
Hang Liu ◽  
Saleha Nisar ◽  
...  
Keyword(s):  
Grape Seed ◽  
Dental Composite ◽  
Cross Linking ◽  
Cranberry Juice ◽  
Composite Restorations ◽  
Dentin Collagen ◽  
Cross Linker ◽  
The Cross ◽  
Demineralized Dentin

Improving the longevity of composite restorations has proven to be difficult when they are bonded to dentin. Dentin demineralization leaves collagen fibrils susceptible to enzymatic digestion, which causes breakdown of the resin–dentin interface. Therefore, measures for counteracting the enzymatic environment by enhancing dentin collagen’s resistance to degradation have the potential to improve the durability of dental composite restorations. This study aimed to evaluate the effects of polyphenol-rich extracts and a chemical cross-linker on the cross-linking interaction, resistance to digestion, and endogenous matrix metalloproteinase (MMP) activities of dentin collagen under clinically relevant conditions. Ten-µm-thick films were cut from dentin slabs of non-carious extracted human third molars. Following demineralization, polyphenol-rich extracts—including grape seed (GSE), green tea (GTE), and cranberry juice (CJE)—or chemical cross-linker carbodiimide with n-hydroxysuccinimide (EDC/NHS) were applied to the demineralized dentin surfaces for 30 s. The collagen cross-linking, bio-stabilization, and gelatinolytic activities of MMPs 2 and 9 were studied by using Fourier-transform infrared spectroscopy, weight loss, hydroxyproline release, scanning/transmission electron microscopy, and in situ zymography. All treatments significantly increased resistance to collagenase degradation and reduced the gelatinolytic MMP activity of dentin collagen compared to the untreated control. The CJE- and GSE-treated groups were more resistant to digestion than the GTE- or EDC/NHS-treated ones (p < 0.05), which was consistent with the cross-linking interaction found with FTIR and the in situ performance on the acid-etched dentin surface found with SEM/TEM. The collagen films treated with CJE showed the lowest MMP activity, followed by GSE, GTE, and, finally, EDC/NHS. The CJE-treated dentin collagen rapidly increased its resistance to digestion and MMP inhibition. An application of CJE as short as 30 s may be a clinically feasible approach to improving the longevity of dentin bonding in composite restorations.

scholarly journals Preparation of Prussian Blue Analogue Ce[Co(CN)6]·nH2O Nanoparticles

2021 ◽  
Vol 3 (6) ◽  
pp. 1-6
Author(s):  
Yajie Guo
Keyword(s):  
Particle Size ◽  
Prussian Blue ◽  
High Efficiency ◽  
Preparation Method ◽  
Low Cost ◽  
Metal Organic Framework ◽  
Prussian Blue Analogue ◽  
Simple Preparation ◽  
Metal Organic ◽  
First Time

Prussian blue analogue Ce[Co(CN)6]·nH2O nanoparticles were prepared by water bath coprecipitation for the first time. The effects of reaction parameters on the morphology and particle size of the synthesized products were revealed by controlling the variable method. The synthetic product has the characteristics of simple preparation method, low cost, small particle size and MOF (Metal organic framework). The cerium and cobalt elements contained in it are common catalytic active elements, which provides ideas and reference for the design and preparation of high-efficiency and low-cost catalysts.

Mechanical Properties and Biocompatibility of in Situ Enzymatically Cross-Linked Gelatin Hydrogels

2017 ◽  
Vol 40 (4) ◽  
pp. 159-168 ◽  
Author(s):  
Nada Z. Alarake ◽  
Patrick Frohberg ◽  
Thomas Groth ◽  
Markus Pietzsch
Keyword(s):  
Mechanical Properties ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Cross Linking ◽  
Adipose Derived Stem Cells ◽  
Swelling Ratio ◽  
Connective Tissues ◽  
Injectable Hydrogels ◽  
Cross Linker

Objectives Gelatin, a degraded collagen, has been widely used as a scaffolding material in tissue engineering applications. In this work, we aimed at the development of in situ, cross-linking, cytocompatible hydrogels by the use of transglutaminase as a cross-linker for potential application in the regeneration of tissues. Methods Hydrogels were prepared from gelatin of different concentrations and bloom degree (175 (G175) or 300 (G300) bloom gelatin) and cross-linked with various amounts of microbial transglutaminase (mTG) at 37°C. Mechanical properties and cross-linking degree were studied by rheology and swelling experiments. Four hydrogels with different stiffness were selected for studies with embedded human adipose-derived stem cells (hASCs). Results Hydrogels were obtained with storage modulus (G’) values between 11 (±1) Pa and 1,800 (±200) Pa with gelation times between 80 (±6) and 450 (±36) seconds. G300 cross-linked gelatin hydrogels displayed higher gel stiffness, lower swelling ratio and gelled more rapidly compared to the hydrogels prepared from G175. Stiffer hydrogels (50 and 200 Pa) showed greater ability to support the proliferation of hASCs than softer ones (11 and 30 Pa). The highest cell proliferation was observed with a hydrogel of 200 Pa modulus. Conclusions Overall, transglutaminase cross-linked gelatin hydrogels might be suitable as injectable hydrogels for the engineering of musculoskeletal and other types of connective tissues.

An injectable hydrogel formed by in situ cross-linking of glycol chitosan and multi-benzaldehyde functionalized PEG analogues for cartilage tissue engineering

2015 ◽  
Vol 3 (7) ◽  
pp. 1268-1280 ◽  
Author(s):  
Luping Cao ◽  
Bin Cao ◽  
Chengjiao Lu ◽  
Guowei Wang ◽  
Lin Yu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Cross Linking ◽  
Glycol Chitosan ◽  
Injectable Hydrogels ◽  
Injectable Hydrogel ◽  
Cross Linker ◽  
Aldehyde Groups

A novel PEG analogue, poly(EO-co-Gly)-CHO, that possesses multiple aldehyde groups is designed and synthesized, and then is used as a cross-linker to react with glycol chitosan to create injectable hydrogels.

scholarly journals Fast and Clean Synthesis of Nylon-6/Synthetic Saponite Nanocomposites

Materials ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 163
Author(s):  
Alejandro Madrid ◽  
Elena Pérez ◽  
Miguel Ángel Vicente ◽  
Vicente Rives ◽  
Raquel Trujillano
Keyword(s):  
Preparation Method ◽  
Low Cost ◽  
Nylon 6 ◽  
Organic Reagents ◽  
Clean Synthesis ◽  
Time Savings ◽  
Energy Reaction ◽  
Environmentally Friendly Method ◽  
Synthetic Saponite

Nylon/saponite nanocomposites were synthesized and characterized. The nanocomposites were prepared by means of a fast, efficient, low cost, and environmentally friendly method. All of the tested preparations resulted in the pre-designed nanocomposites. To this end, delaminated saponites were directly synthesized to be used as a filler in a polymer matrix formed by nylon-6 by the in situ intercalation polymerisation of an ε-caprolactam monomer without the use of surfactants or other organic reagents to organophilise the clay, thus avoiding the drawbacks of contamination. The efficiency of the preparation method increased, and significant savings were achieved in terms of both energy reaction time, savings of 60% and 70%, respectively, by using microwave radiation as an energy source during the synthesis of the nanocomposites. In addition, given that the method that was followed avoids the use of contaminating organophilic agents, it is important to highlight the savings in reagents and the fact that there was zero environmental contamination.

A Novel Capacitive Negative Electrode Material of Fe3N

NANO ◽  
2018 ◽  
Vol 13 (01) ◽  
pp. 1850002 ◽  
Author(s):  
Zhi-Yun Zhao ◽  
Wei-Bin Zhang ◽  
Xue-Jing Ma ◽  
Kai Li ◽  
Yue Zhao ◽  
...  
Keyword(s):  
Current Density ◽  
Electrode Material ◽  
Preparation Method ◽  
Low Cost ◽  
Negative Electrode ◽  
Initial Value ◽  
Capacitive Energy Storage ◽  
Simple Preparation ◽  
Negative Electrode Material ◽  
A Current

A capacitive negative electrode material of Fe3N has been synthesized via a simple precursor ammoniation treatment. Electrochemical measure shows that the electrode exhibits a specific capacitance of 270[Formula: see text]F[Formula: see text]g[Formula: see text] at a current density of 0.5[Formula: see text]A[Formula: see text]g[Formula: see text] in 6[Formula: see text]M KOH, 66% of the initial value remains at a specific current density of 5[Formula: see text]A[Formula: see text]g[Formula: see text]. Capacitance retention after 5000 cycles is about 61.5% of the initial capacitance. The electrochemical performance is relatively great in comparison with the literature reports of some Fe-based compounds. Because of the low cost and simple preparation method of Fe3N, the material is promising in electrochemical capacitive energy storage applications.

Synthesis and Antibacterial Activity of Silver Nanoparticles Embedded in Smart Poly(N-Isopropylacrylamide)-Based Hydrogel Networks

2011 ◽  
Vol 2 (4) ◽  
Author(s):  
Fereshteh Valipour ◽  
Majid Esmhosseini ◽  
Kamelia Nejati ◽  
Hasan Kianfar ◽  
Ardalan Pasdaran ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Methacrylic Acid ◽  
Cross Linking ◽  
Hydroxyethyl Methacrylate ◽  
In Situ Reduction ◽  
Vis Spectroscopy ◽  
Cross Linker ◽  
Triethyleneglycol Dimethacrylate

In recent study, we report the synthesis and antibacterial activity of silver nanoparticles embedded in smart poly(N-isopropylacrylamide)-based hydrogel networks. A series of thermosensitive poly(N-isopropylacrylamide-methacrylic acid-hydroxyethyl methacrylate) [P(NIPAAm-MAA-HEM)] with various cross-linking ratio have been obtained by cross-linking free radical polymerization of N-isopropylacrylamide (NIPAAm), methacrylic acid (MAA), and hydroxyethyl methacrylate (HEM) in the presence of triethyleneglycol dimethacrylate (TEGDMA) as cross-linker. Highly stable and uniformly distributed silver nanoparticles have been obtained with hydrogel networks via in situ reduction of silver nitrate (AgNO3) using sodium borohydride (NaBH4) as reducing agent. The formation of silver nanoparticles has been confirmed with ultraviolet visible (UV–Vis) spectroscopy. Scanning electron microscopy (SEM) results demonstrated that employed hydrogels have regulated the silver nanoparticles size to 50–150 nm. The preliminary antibacterial activity performed to these hydrogel–silver nanocomposites.

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