Mechanical Properties and Biocompatibility of in Situ Enzymatically Cross-Linked Gelatin Hydrogels

2017 ◽  
Vol 40 (4) ◽  
pp. 159-168 ◽  
Author(s):  
Nada Z. Alarake ◽  
Patrick Frohberg ◽  
Thomas Groth ◽  
Markus Pietzsch
Keyword(s):  
Mechanical Properties ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Cross Linking ◽  
Adipose Derived Stem Cells ◽  
Swelling Ratio ◽  
Connective Tissues ◽  
Injectable Hydrogels ◽  
Cross Linker

Objectives Gelatin, a degraded collagen, has been widely used as a scaffolding material in tissue engineering applications. In this work, we aimed at the development of in situ, cross-linking, cytocompatible hydrogels by the use of transglutaminase as a cross-linker for potential application in the regeneration of tissues. Methods Hydrogels were prepared from gelatin of different concentrations and bloom degree (175 (G175) or 300 (G300) bloom gelatin) and cross-linked with various amounts of microbial transglutaminase (mTG) at 37°C. Mechanical properties and cross-linking degree were studied by rheology and swelling experiments. Four hydrogels with different stiffness were selected for studies with embedded human adipose-derived stem cells (hASCs). Results Hydrogels were obtained with storage modulus (G’) values between 11 (±1) Pa and 1,800 (±200) Pa with gelation times between 80 (±6) and 450 (±36) seconds. G300 cross-linked gelatin hydrogels displayed higher gel stiffness, lower swelling ratio and gelled more rapidly compared to the hydrogels prepared from G175. Stiffer hydrogels (50 and 200 Pa) showed greater ability to support the proliferation of hASCs than softer ones (11 and 30 Pa). The highest cell proliferation was observed with a hydrogel of 200 Pa modulus. Conclusions Overall, transglutaminase cross-linked gelatin hydrogels might be suitable as injectable hydrogels for the engineering of musculoskeletal and other types of connective tissues.

OSTEOGENESIS OF ADIPOSE-DERIVED STEM CELLS ON THREE-DIMENSIONAL, MACROPOROUS GELATIN–HYALURONIC ACID CRYOGELS

2011 ◽  
Vol 23 (02) ◽  
pp. 127-133 ◽  
Author(s):  
Liao Han Tsung ◽  
Kun-Hung Chang ◽  
Jyh Ping Chen
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Hyaluronic Acid ◽  
Pore Size ◽  
Three Dimensional ◽  
Early Gene ◽  
Osteogenic Potential ◽  
Porous Scaffolds ◽  
Adipose Derived Stem Cells ◽  
Swelling Ratio

Aim. Macroporous sponge-like gelatin–hyaluronic acid (Gl–HA) scaffolds cross-linked by EDC were produced using cryogelation technology, which allows for the preparation of highly porous scaffolds without compromising their mechanical properties, and is a more cost-efficient process than freeze drying. The aim of this study is to evaluate the osteogenic potential of porcine adipose-derived stem cells (PADSCs) in GI–HA cryogel. Method. The character of the GI–HA cryogel was evaluated. The pore size and the microstructure were observed using scanning electron microscope (SEM). The swelling ratio was measured. The PADSCs were harvested and isolated from pig inguinal area. Then, the GI–HA cryogel was seeded with PADSCs. The cryogel/ASCs mixture was cultured in osteogenic medium for 0, 3, 7, 14, and 21 days. The cell proliferation was measured by MTS. The RT-PCR of specific osteogenic gene expression such as osteocalcin (OC), RUNX2 was used to assess the osteogenic ability. The SEM was used to observe the interaction between scaffold and cells. Energy dispersive spectrum (EDS) was used to analyze the mineralization around cells. Results. The pore size was variable between 200 and 369 μm. The swelling ratio was around 8.67 ± 1.669%. The cell proliferation was increasing along with the increase of induction periods. The expression of early gene of RUNX2 and late gene of OC mean that the PADSCs were differentiated well into osteoblasts within the cryogels. The SEM detailed that the PADSCs cell can proliferate well in the pore of GI–HA scaffold. The EDS also demonstrated the mineralization of PADSCs in GI–HA scaffold after induction. Conclusions. To conclude, the PADSCs can proliferate and differentiate well into osteoblasts in the three-dimensional, porous, GI–HA cryogel.

An injectable hydrogel formed by in situ cross-linking of glycol chitosan and multi-benzaldehyde functionalized PEG analogues for cartilage tissue engineering

2015 ◽  
Vol 3 (7) ◽  
pp. 1268-1280 ◽  
Author(s):  
Luping Cao ◽  
Bin Cao ◽  
Chengjiao Lu ◽  
Guowei Wang ◽  
Lin Yu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Cross Linking ◽  
Glycol Chitosan ◽  
Injectable Hydrogels ◽  
Injectable Hydrogel ◽  
Cross Linker ◽  
Aldehyde Groups

A novel PEG analogue, poly(EO-co-Gly)-CHO, that possesses multiple aldehyde groups is designed and synthesized, and then is used as a cross-linker to react with glycol chitosan to create injectable hydrogels.

scholarly journals Engineering of injectable hydrogels associate with Adipose-Derived stem cells delivery for anti-cardiac hypertrophy agents

Drug Delivery ◽  
2021 ◽  
Vol 28 (1) ◽  
pp. 1334-1341
Author(s):  
Guangyu Long ◽  
Quanhe Wang ◽  
Shaolin Li ◽  
Junzhong Tao ◽  
Boyan Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cardiac Hypertrophy ◽  
Adipose Derived Stem Cells ◽  
Injectable Hydrogels

scholarly journals Porous Cellulose-Collagen Scaffolds For Soft Tissue Regeneration: Influence of Cellulose Derivatives On Mechanical Properties And Compatibility With Adipose-Derived Stem Cells.

2022 ◽  
Author(s):  
Katarína Kacvinská ◽  
Martina Trávničková ◽  
Lucy Vojtová ◽  
Petr Poláček ◽  
Jana Dorazilová ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Adhesion ◽  
Soft Tissue ◽  
Vascular Tissue ◽  
Biological Properties ◽  
Cellulose Derivatives ◽  
Adipose Derived Stem Cells ◽  
Soft Tissue Regeneration

Abstract This study deals with cellulose derivatives in relation to the collagen fibrils in composite collagen-cellulose scaffolds for soft tissue engineering. Two types of cellulose, i.e., oxidized cellulose (OC) and carboxymethyl cellulose (CMC), were blended with collagen (Col) to enhance its elasticity, stability and sorptive biological properties, e.g. hemostatic and antibacterial features. The addition of OC supported the resistivity of the Col fibrils in a dry environment, while in a moist environment OC caused a radical drop. The addition of CMC reduced the mechanical strength of the Col fibrils in both environments. The elongation of the Col fibrils was increased by both types of cellulose derivatives in both environments, which is closely related to tissue like behaviour. In these various mechanical environments, the ability of human adipose-derived stem cells (hADSCs) to adhere and proliferate was significantly greater in the Col and Col/OC scaffolds than in the Col/CMC scaffold. This is explained by deficient mechanical support and loss of stiffness due to the high swelling capacity of CMC. Although Col/OC and Col/CMC acted differently in terms of mechanical properties, both materials were observed to be cytocompatible, with varying degrees of further support for cell adhesion and proliferation. While Col/OC can serve as a scaffolding material for vascular tissue engineering and for skin tissue engineering, Col/CMC seems to be more suitable for moist wound healing, e.g. as a mucoadhesive gel for exudate removal, since there was almost no cell adhesion.

scholarly journals Long non-coding RNA FAM83H-AS1 induced by adipose-derived stem cells promotes breast and pancreatic cancer cell proliferation and migration

2020 ◽  
Author(s):  
Jianing Tang ◽  
Qiuxia Cui ◽  
Dan Zhang ◽  
Xing Liao ◽  
Yan Gong ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Adipose Derived Stem Cells ◽  
Unfolded Protein ◽  
Pancreatic Cancer Cells ◽  
Cell Proliferation And Migration ◽  
And Migration ◽  
Protein Response ◽  
Proliferation And Migration

Abstract Background Stromal cells recruited to the tumor microenvironment and long non-coding RNAs (lncRNAs) in the tumor cells regulate cancer progression. However, their relationship is largely unknown. Methods In the current study, we identified the effects of lncRNA FAM83H-AS1, induced by adipose-derived stem cells (ADSCs) during tumor development, and explored the underlying mechanisms using a coculture cell model. Adipose tissues were obtained from healthy female donors, the expression of stromal markers on cell surface of expanded ADSCs were confirmed using immunofluorescence analysis. The breast and pancreatic cancer cells were cultured with or without ADSCs using 24-well transwell chamber systems with 8.0 µm pore size. Results Our results showed that FAM83H-AS1 was upregulated in breast and pancreatic cancers and associated with poor prognosis. ADSCs further induced FAM83H-AS1 and increased tumor cell proliferation via promoting G1/S transition through cyclin D1, CDK4 and CDK6. Wound healing, modified Boyden chamber and immunoblotting assays demonstrated that ADSCs induced epithelial-mesenchymal transition and migration of breast and pancreatic cancer cells in a FAM83H-AS1-dependent manner. And ADSC-induced FAM83H-AS1 increased unfolded protein response through AKT/XBP1 pathway. Conclusion In conclusion, our results indicated that ADSCs promoted breast and pancreatic cancer development via inducing cell proliferation and migration, as well as unfolded protein response through FAM83H-AS1.

scholarly journals Dynamic cross-linking of an alginate–acrylamide tough hydrogel system: time-resolved in situ mapping of gel self-assembly

RSC Advances ◽  
2021 ◽  
Vol 11 (18) ◽  
pp. 10710-10726
Author(s):  
Akanksha Pragya ◽  
Suhas Mutalik ◽  
Muhammad Waseem Younas ◽  
Siu-Kwong Pang ◽  
Pui-Kin So ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Formation Process ◽  
Self Assembly ◽  
Cross Linking ◽  
Time Resolved ◽  
System Time ◽  
Dynamic Formation ◽  
Hydrogel System

In situ, time-resolved characterisation of an alginate–acrylamide tough hydrogel dynamic formation process indicate routes to intervention and modification of chemo-physico-mechanical properties.

scholarly journals Low-level laser irradiation promotes proliferation of cryopreserved adipose-derived stem cells

2017 ◽  
Vol 15 (3) ◽  
pp. 334-338 ◽  
Author(s):  
Fernanda Ginani ◽  
Diego Moura Soares ◽  
Hugo Alexandre de Oliveira Rocha ◽  
Carlos Augusto Galvão Barboza
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Cell Proliferation ◽  
Laser Irradiation ◽  
Adipose Derived Stem Cells ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Significant Difference ◽  
Low Level Laser Irradiation

ABSTRACT Objective To evaluate the effect of low-level laser irradiation on proliferation and viability of murine adipose-derived stem cells previously submitted to cryopreservation. Methods Adipose-derived stem cells were isolated from inguinal fat pads of three mice, submitted to cryopreservation in fetal bovine serum with 10% dimethylsulfoxide for 30 days and then thawed and maintained in normal culture conditions. Culture cells were either irradiated or not (control) with an InGaAIP diode laser at zero and 48 hours, using two different energy densities (0.5 and 1.0J/cm2). Cell proliferation was evaluated by trypan blue exclusion method and MTT assay at intervals of zero, 24, 48, and 72 hours after the first laser application. Cell viability and apoptosis of previously cryopreserved cells submitted to laser therapy were evaluated by flow cytometry. Results The Irradiated Groups (0.5 and 1.0J/cm2) showed an increased cell proliferation (p<0.05) when compared to the Control Group, however no significant difference between the two energy densities was observed. Flow cytometry revealed a percentage of viable cells higher than 99% in all groups. Conclusion Low-level laser irradiation has stimulatory effects on the proliferation of adipose-derived stem cells previously submitted to cryopreservation.

scholarly journals PVA and CS cross-linking combined with in situ chimeric SiO2 nanoparticle adhesion to enhance the hydrophilicity and antibacterial properties of PTFE flat membranes

RSC Advances ◽  
2019 ◽  
Vol 9 (33) ◽  
pp. 19205-19216 ◽  
Author(s):  
Chengcai Li ◽  
Hang Zhang ◽  
Feng Wang ◽  
Hailin Zhu ◽  
Yuhai Guo ◽  
...  
Keyword(s):  
Preparation Method ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Cross Linking ◽  
Sio2 Nanoparticle ◽  
Simple Preparation ◽  
Cross Linker ◽  
Flat Membranes

Herein, a new hydrophilic and antibacterial PTFE flat MF membrane was fabricated via a low-cost and simple preparation method in which CS was crosslinked with PVA using ECH as a cross-linker followed by in situ chimeric SiO2 nanoparticle adhesion.

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