Ti3C2Tx, a new type of two-dimensional material, is a prospective anode material in lithium-ion batteries (LIBs) for its low lithium-ion diffusion barrier, high conductivity and many other excellent properties. In this paper, multilayer Ti3C2Tx and delaminated Ti3C2Tx samples are prepared by etching Ti3AlC2 powder with HF and [Formula: see text], respectively. We explore the application of the two samples in LIBs, and analyze their electrochemical behavior and kinetic mechanism. At the current densities of 0.1[Formula: see text]A[Formula: see text]g[Formula: see text], the delaminated Ti3C2Tx electrode delivered higher capacities of 255[Formula: see text]mAh[Formula: see text]g[Formula: see text] than multilayer Ti3C2Tx electrode (100[Formula: see text]mAh[Formula: see text]g[Formula: see text]). Even after 1000 cycles, the specific capacity of the delaminated Ti3C2Tx is still up to 205[Formula: see text]mAh[Formula: see text]g[Formula: see text] at 1[Formula: see text]A[Formula: see text]g[Formula: see text]. This work proves the great potential of the delaminated Ti3C2Tx for lithium-ion storage.