Perylene diimide–Cu2+ based fluorescent nanoparticles for the detection of spermine in clinical and food samples: a step toward the development of a diagnostic kit as a POCT tool for spermine

2019 ◽  
Vol 7 (45) ◽  
pp. 7218-7227 ◽  
Author(s):  
Kapil Kumar ◽  
Sandeep Kaur ◽  
Satwinderjeet Kaur ◽  
Gaurav Bhargava ◽  
Subodh Kumar ◽  
...  
Keyword(s):  
Real Time ◽  
Cost Effective ◽  
Food Samples ◽  
Fermented Food ◽  
Perylene Diimide ◽  
Fluorescent Nanoparticles ◽  
Solution Form ◽  
Cost Effective Method ◽  
Real Time Detection

EA-PDI∩Cu2+ complex can be established as cost-effective method to develop diagnostic kit for POCT of spermine for real time detection of spermine in vapor and solution form released from fermented food samples.

scholarly journals From Village to Globe: A Dynamic Real-Time Map of African Fields Through PlantVillage

2021 ◽  
Vol 5 ◽  
Author(s):  
Annalyse Kehs ◽  
Peter McCloskey ◽  
John Chelal ◽  
Derek Morr ◽  
Stellah Amakove ◽  
...  
Keyword(s):  
Real Time ◽  
Ground Truth ◽  
Cost Effective ◽  
Quality Data ◽  
Learning Tools ◽  
High Quality ◽  
Ground Truth Data ◽  
Cost Effective Method ◽  
High Throughput Method ◽  
Major Bottleneck

A major bottleneck to the application of machine learning tools to satellite data of African farms is the lack of high-quality ground truth data. Here we describe a high throughput method using youth in Kenya that results in a cost-effective method for high-quality data in near real-time. This data is presented to the global community, as a public good and is linked to other data sources that will inform our understanding of crop stress, particularly in the context of climate change.

scholarly journals An all-solid-state heterojunction oxide transistor for the rapid detection of biomolecules and SARS-CoV-2 spike S1 protein

2021 ◽  
Author(s):  
Yen-Hung Lin ◽  
Yang Han ◽  
Abhinav Sharma ◽  
Wejdan S. AlGhamdi ◽  
Chien-Hao Liu ◽  
...  
Keyword(s):  
Solid State ◽  
Real Time ◽  
High Sensitivity ◽  
Cost Effective ◽  
High Electron ◽  
Real Time Detection ◽  
Bioanalytical Applications ◽  
Detection Of Biomolecules ◽  
Dimensional Electron Gas ◽  
Sensitivity Specificity

AbstractSolid-state transistor sensors that can detect biomolecules in real time are highly attractive for emerging bioanalytical applications. However, combining cost-effective manufacturing with high sensitivity, specificity and fast sensing response, remains challenging. Here we develop low-temperature solution-processed In2O3/ZnO heterojunction transistors featuring a geometrically engineered tri-channel architecture for rapid real-time detection of different biomolecules. The sensor combines a high electron mobility channel, attributed to the quasi-two-dimensional electron gas (q2DEG) at the buried In2O3/ZnO heterointerface, in close proximity to a sensing surface featuring tethered analyte receptors. The unusual tri-channel design enables strong coupling between the buried q2DEG and the minute electronic perturbations occurring during receptor-analyte interactions allowing for robust, real-time detection of biomolecules down to attomolar (aM) concentrations. By functionalizing the tri-channel surface with SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) antibody receptors, we demonstrate real-time detection of the SARS-CoV-2 spike S1 protein down to attomolar concentrations in under two minutes.

A simplified and cost-effective method combining real-time PCR and pyrosequencing for detection of aac(6′)-Ib-cr gene

2013 ◽  
Vol 95 (2) ◽  
pp. 268-271 ◽  
Author(s):  
Thomas Guillard ◽  
Nicolas Fontaine ◽  
Anne Limelette ◽  
Anne-Laure Lebreil ◽  
Janick Madoux ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Cost Effective ◽  
Cost Effective Method

scholarly journals Development and evaluation of a multiplex reverse-transcription real-time PCR assay for detection of equine respiratory disease viruses

2018 ◽  
Vol 30 (6) ◽  
pp. 924-928
Author(s):  
Shimaa M. Ghoniem ◽  
Ayman H. El Deeb ◽  
Mohammed G. Aggour ◽  
Hussein A. Hussein
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Influenza A ◽  
Simultaneous Detection ◽  
Cost Effective ◽  
Equine Influenza Virus ◽  
Equine Influenza ◽  
Cost Effective Method ◽  
Nonspecific Interactions

We developed a multiplex reverse-transcription real-time PCR (RT-rtPCR) assay for the simultaneous detection of the main equine respiratory viruses: equid alphaherpesviruses 1 and 4 (EHV-1, -4) and equine influenza virus (EIV; species Influenza A virus). The primers and probes amplified only the targeted viruses, and there were no inter-assay cross-amplifications or nonspecific interactions. The multiplex assay efficiencies were 92.5%, 97%, and 90% for EHV-1, EHV-4, and EIV, respectively. The R2 values of the monoplex and multiplex assays were ⩾0.990, and the slopes were −3.37 to −3.59. The performance of the assay was evaluated by analyzing 152 samples from clinically infected horses. EHV-1 DNA was detected in 12 samples, EHV-4 DNA in 9 samples, and both EHV-1 and EHV-4 in 4 samples. The accuracy of the assay was confirmed by comparing these results using commercial rtPCR and RT-rtPCR kits. Our multiplex RT-rtPCR was a sensitive, specific, accurate, and cost-effective method for the detection of the target viruses whether they occur alone or as part of coinfections.

HPV Detection and Genotyping Using the Luminex xMAP Technology

2010 ◽  
pp. 83-87
Author(s):  
Marios Kambouris ◽  
Vasiliki Chini ◽  
Andriani Daskalaki
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Cost Effective ◽  
Suspension Array ◽  
Major Disadvantage ◽  
Cost Effective Method ◽  
Array Technology ◽  
Hpv Genotypes ◽  
Affected Tissue ◽  
Epithelial Layers

The Human Papillomavirus (HPV) is playing an important role in oral cancer. The molecular detection of HPV is based on the fact that the viral DNA is present in all the epithelial layers of the affected tissue and it can be detected easily with PCR or Real-Time PCR. The major disadvantage of PCR is that it cannot provide genotype information and the Real-Time PCR can only detect very few types in a multiplex assay. Although the HPV typing assays are capable of typing a relatively large spectrum of HPV genotypes, they cannot be automated or deployed in a high-throughput platform. The bead-based technology (Luminex suspension array technology) provides a rapid and cost-effective method to simultaneously detect different HPV genotypes.

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