A poly(N,N-dimethylaminoethyl methacrylate-co-ethylene glycol dimethacrylate) monolith for direct solid-phase extraction of benzodiazepines from undiluted human urine

2020 ◽  
Vol 12 (31) ◽  
pp. 3924-3932
Author(s):  
Tengwen Zhao ◽  
Li Du ◽  
Ziyang Zhang ◽  
Na Li ◽  
Manman Wang ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Solid Phase Extraction ◽  
Human Urine ◽  
Solid Phase ◽  
Ethylene Glycol Dimethacrylate ◽  
Urine Samples ◽  
Phase Extraction ◽  
Glycol Dimethacrylate ◽  
Dimethylaminoethyl Methacrylate ◽  
Direct Solid

A poly(N,N-dimethylaminoethyl methacrylate-co-ethylene glycol dimethacrylate) monolith was successfully synthesized and applied for direct solid-phase extraction of benzodiazepines from undiluted urine samples.

scholarly journals Proteins Pre-concentration Using Glycidyl Methacrylate-co-stearyl Methacrylate-co-ethylene Glycol Dimethacrylate Monolith

2020 ◽  
Vol 20 (5) ◽  
pp. 1143
Author(s):  
Ahmed Ali Alkarimi ◽  
Kevin Welham
Keyword(s):  
Cytochrome C ◽  
Ethylene Glycol ◽  
Solid Phase Extraction ◽  
Glycidyl Methacrylate ◽  
Solid Phase ◽  
Ethylene Glycol Dimethacrylate ◽  
Phase Extraction ◽  
Glycol Dimethacrylate ◽  
Stearyl Methacrylate ◽  
Monolithic Sorbent

Solid-phase extraction technique is considered as a vital tool in a pre-treatment of different samples. Therefore, it was used as a beneficial and alternative extraction method over the liquid–liquid extraction in diverse applications such as food, environmental, and biological analyses. Glycidyl methacrylate-co-stearyl methacrylate-co-ethylene glycol dimethacrylate (GMA-co-SMA-co-EDMA) monolithic sorbent was prepared as strong cationic-reversed phase sorbent for solid-phase extraction of different proteins. The monolithic sorbent was prepared inside two moulds such as glass microchip device and borosilicate. The morphological properties have been investigated using the Brunauer-Emmett-Teller (BET) model analyzer and scanning electron microscope (SEM), and the formation of the monolithic sorbent was examined using the FT-IR. Eight proteins (cytochrome C, insulin, myoglobin, lysozyme, β-lactoglobulin, trypsin, albumin chicken egg white, and apo-transferrin) with different molecular weight and isoelectric point were investigated for pre-concentration using the SCX/RP sorbent. It was found that the highest extraction recovery was obtained with three proteins cytochrome C, lysozyme, and myoglobin compared with the other proteins. These three proteins were chosen for pre-concentration using the glass microchip. Sorbent performance showed significant results in terms of stability and reproducibility.

scholarly journals Application of Solid Phase Extraction and High-Performance Liquid Chromatography with Fluorescence Detection to Analyze Bisphenol A Bis (2,3-Dihydroxypropyl) Ether (BADGE 2H2O), Bisphenol F (BPF), and Bisphenol E (BPE) in Human Urine Samples

2021 ◽  
Vol 18 (19) ◽  
pp. 10307
Author(s):  
Tomasz Tuzimski ◽  
Szymon Szubartowski
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Human Urine ◽  
High Performance ◽  
Solid Phase ◽  
Urine Samples ◽  
Phase Extraction ◽  
Human Urine Samples ◽  
Bisphenol F

In this study, we propose a simple, cost-effective, and sensitive high-performance liquid chromatography method with fluorescence detection (HPLC-FLD) for the simultaneous determination of the three bisphenols (BPs): bisphenol A bis (2,3-dihydroxypropyl) ether (BADGE 2H2O), bisphenol F (BPF), and bisphenol E (BPE) in human urine samples. The dispersive solid phase extraction (d-SPE) coupled with solid phase extraction (SPE) procedure performed well for the analytes with recoveries in the range of 74.3–86.5% and relative standard deviations (RSD%) less than 10%. The limits of quantification (LOQs) for all investigated analytes were in the range of 11.42–22.35 ng mL−1. The method was validated at three concentration levels (1 × LOQ, 1.5 × LOQ, and 3 LOQ). During the bisphenols HPLC-FLD analysis, from 6 min a reinforcement (10 or 12) was used, therefore analytes might be identified in the small volume human urine samples. The results demonstrated clearly that the approach developed provides reliable, simple, and rapid quantification and identification of three bisphenols in a urine matrix and could be used for monitoring these analytes.

Solid-Phase Extraction Techniques for Assay of Diuretics in Human Urine Samples

1991 ◽  
Vol 14 (19) ◽  
pp. 3575-3590 ◽  
Author(s):  
Pilar Campins-falcó ◽  
Rosa Herráez-Hernández ◽  
Adela Sevillano-cabeza
Keyword(s):  
Solid Phase Extraction ◽  
Human Urine ◽  
Solid Phase ◽  
Urine Samples ◽  
Phase Extraction ◽  
Extraction Techniques ◽  
Human Urine Samples
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