scholarly journals Trophic transfer of CuO NPs from sediment to worms (Tubifex tubifex) to fish (Gasterosteus aculeatus): a comparative study of dissolved Cu and NPs enriched with a stable isotope tracer (65Cu)

2020 ◽  
Vol 7 (8) ◽  
pp. 2360-2372
Author(s):  
Tobias Lammel ◽  
Amalie Thit ◽  
Xianjin Cui ◽  
Catherine Mouneyrac ◽  
Anders Baun ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Gasterosteus Aculeatus ◽  
Trophic Transfer ◽  
Copper Oxide Nanoparticles ◽  
Tubifex Tubifex ◽  
Oxide Nanoparticles ◽  
Stable Isotope Tracer ◽  
Isotope Tracer ◽  
Cuo Nps ◽  
Transfer Potential

This study used stable isotope-labeled copper oxide nanoparticles (65CuO NPs) to determine their trophic transfer potential from sediment to worms to fish at environmentally realistic exposure doses and compared to dissolved Cu (65CuCl2).

scholarly journals Influence of Aging on Bioaccumulation and Toxicity of Copper Oxide Nanoparticles and Dissolved Copper in the Sediment-Dwelling Oligochaete Tubifex tubifex: A Long-Term Study Using a Stable Copper Isotope

2021 ◽  
Vol 3 ◽  
Author(s):  
Amalie Thit ◽  
Monica Hamann Sandgaard ◽  
Joachim Sturve ◽  
Catherine Mouneyrac ◽  
Anders Baun ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Adverse Effects ◽  
Copper Oxide ◽  
Body Burden ◽  
Copper Oxide Nanoparticles ◽  
Tubifex Tubifex ◽  
High Background ◽  
Cuo Nps ◽  
Long Term Study ◽  
Spiked Sediment

For engineered metal nanoparticles (NPs), such as copper oxide (CuO) NPs, the sediment is recognized as a major compartment for NP accumulation. Sediment-dwelling organisms, such as the worm Tubifex tubifex, will be at particular risk of metal and metal NP exposure. However, a range of complex transformation processes in the sediment affects NP bioavailability and toxicity as the contamination ages. The objective of this study was to examine bioaccumulation and adverse effects of CuO NPs in T. tubifex compared to dissolved Cu (administered as CuCl2) and the influence of aging of spiked sediment. This was done in a 28-day exposure experiment with T. tubifex incubated in clean sediment or freshly spiked sediment with different concentrations of dissolved Cu (up to 230 μg g−1 dw) or CuO NPs (up to 40 μg g−1 dw). The experiment was repeated with the same sediments after it had been aged for 2 years. To obtain a distinct isotopic signature compared to background Cu, both Cu forms were based on the stable isotope 65Cu (>99%). The 28-day exposure to sediment-associated dissolved 65Cu and 65CuO NPs resulted in a clear concentration-dependent increase in the T. tubifex65Cu body burden. However, despite the elevated 65Cu body burdens in exposed worms, limited adverse effects were observed in either of the two experiments (e.g., above 80% survival in all treatments, low or no effects on the growth rate, feeding rate, and reproduction). Organisms exposed to aged sediments had lower body burdens of 65Cu than those exposed to freshly spiked sediments and we suggest that aging decreases the bioavailability of both 65Cu forms. In this study, the use of a stable isotope made it possible to use environmentally realistic Cu concentrations and, at the same time, differentiate between newly accumulated 65Cu and background Cu in experimental samples despite the high background Cu concentrations in sediment and T. tubifex tissue. Realistic exposure concentrations and aging of NPs should preferably be included in future studies to increase environmental realism to accurately predict the environmental risk of metal NPs.

ANTIBIOTIC PROFILE AND ANTIBACTERIAL ACTIVITY OF COPPER OXIDE NANOPARTICLES AGAINST Pseudomonas fluorescens ISOLATED FROM WOUND INFECTION

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Haider Qassim Raheem ◽  
Takwa S. Al-meamar ◽  
Anas M. Almamoori
Keyword(s):  
Antibacterial Activity ◽  
Copper Oxide ◽  
Pseudomonas Fluorescens ◽  
Wide Spectrum ◽  
Copper Oxide Nanoparticles ◽  
Morphological Properties ◽  
Clinical Samples ◽  
Oxide Nanoparticles ◽  
Cuo Nps ◽  
Disk Diffusion Assay

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.

scholarly journals Green copper oxide nanoparticles for lead, nickel, and cadmium removal from contaminated water

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alaa El Din Mahmoud ◽  
Khairia M. Al-Qahtani ◽  
Sahab O. Alflaij ◽  
Salma F. Al-Qahtani ◽  
Faten A. Alsamhan
Keyword(s):  
Experimental Data ◽  
Copper Oxide ◽  
Copper Oxide Nanoparticles ◽  
Bet Surface Area ◽  
Oxide Nanoparticles ◽  
Cadmium Removal ◽  
Cuo Nps ◽  
Pseudo Second Order ◽  
Isotherm And Kinetic Models ◽  
Lead Nickel

AbstractEnvironmentally friendly copper oxide nanoparticles (CuO NPs) were prepared with a green synthesis route without using hazardous chemicals. Hence, the extracts of mint leaves and orange peels were utilized as reducing agents to synthesize CuO NPs-1 and CuO NPs-2, respectively. The synthesized CuO NPs nanoparticles were characterized using scanning electron microscopy (SEM), Energy Dispersive X-ray Analysis (EDX), BET surface area, Ultraviolet–Visible spectroscopy (UV–Vis), and Fourier Transform Infrared Spectroscopy (FT-IR). Various parameters of batch experiments were considered for the removal of Pb(II), Ni(II), and Cd(II) using the CuO NPs such as nanosorbent dose, contact time, pH, and initial metal concentration. The maximum uptake capacity (qm) of both CuO NPs-1 and CuO NPs-2 followed the order of Pb(II) > Ni(II) > Cd(II). The optimum qm of CuO NPs were 88.80, 54.90, and 15.60 mg g−1 for Pb(II), Ni(II), and Cd(II), respectively and occurred at sorbent dose of 0.33 g L−1 and pH of 6. Furthermore, isotherm and kinetic models were applied to fit the experimental data. Freundlich models (R2 > 0.97) and pseudo-second-order model (R2 > 0.96) were fitted well to the experimental data and the equilibrium of metal adsorption occurred within 60 min.

scholarly journals Green Synthesis of Copper Oxide Nanoparticles Using Protein Fractions from an Aqueous Extract of Brown Algae Macrocystis pyrifera

Processes ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 78
Author(s):  
Karla Araya-Castro ◽  
Tzu-Chiao Chao ◽  
Benjamín Durán-Vinet ◽  
Carla Cisternas ◽  
Gustavo Ciudad ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Copper Oxide ◽  
Green Synthesis ◽  
Aqueous Extract ◽  
Brown Algae ◽  
Copper Oxide Nanoparticles ◽  
Macrocystis Pyrifera ◽  
Protein Fractions ◽  
Oxide Nanoparticles ◽  
Cuo Nps

Amongst different living organisms studied as potential candidates for the green synthesis of copper nanoparticles, algal biomass is presented as a novel and easy-to-handle method. However, the role of specific biomolecules and their contribution as reductant and capping agents has not yet been described. This contribution reports a green synthesis method to obtain copper oxide nanoparticles (CuO-NPs) using separated protein fractions from an aqueous extract of brown algae Macrocystis pyrifera through size exclusion chromatography (HPLC-SEC). Proteins were detected by a UV/VIS diode array, time-based fraction collection was carried out, and each collected fraction was used to evaluate the synthesis of CuO-NPs. The characterization of CuO-NPs was evaluated by Dynamic Light Scattering (DLS), Z-potential, Fourier Transform Infrared (FTIR), Transmission Electron Microscope (TEM) equipped with Energy Dispersive X-ray Spectroscopy (EDS) detector. Low Molecular Weight (LMW) and High Molecular Weight (HMW) protein fractions were able to synthesize spherical CuO-NPs. TEM images showed that the metallic core present in the observed samples ranged from 2 to 50 nm in diameter, with spherical nanostructures present in all containing protein samples. FTIR measurements showed functional groups from proteins having a pivotal role in the reduction and stabilization of the nanoparticles. The highly negative zeta potential average values from obtained nanoparticles suggest high stability, expanding the range of possible applications. This facile and novel protein-assisted method for the green synthesis of CuO-NPs may also provide a suitable tool to synthesize other nanoparticles that have different application areas.

scholarly journals Nitrogen spiraling in streams: Comparisons between stable isotope tracer and nutrient addition experiments

2007 ◽  
Vol 52 (4) ◽  
pp. 1718-1723 ◽  
Author(s):  
Stevan R. Earl ◽  
H. Maurice Valett ◽  
Jackson R. Webster
Keyword(s):  
Stable Isotope ◽  
Nutrient Addition ◽  
Stable Isotope Tracer ◽  
Isotope Tracer

scholarly journals Positional Enrichment by Proton Analysis (PEPA): A One-Dimensional 1 H-NMR Approach for 13 C Stable Isotope Tracer Studies in Metabolomics

2017 ◽  
Vol 56 (13) ◽  
pp. 3531-3535 ◽  
Author(s):  
Maria Vinaixa ◽  
Miguel A. Rodríguez ◽  
Suvi Aivio ◽  
Jordi Capellades ◽  
Josep Gómez ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Tracer Studies ◽  
Stable Isotope Tracer ◽  
One Dimensional ◽  
Isotope Tracer ◽  
H Nmr

Exploitation of a deep-water algal maximum by Daphnia: a stable-isotope tracer study

2003 ◽  
pp. 95-101
Author(s):  
Winfried Lampert ◽  
Jonathan Grey
Keyword(s):  
Stable Isotope ◽  
Deep Water ◽  
Tracer Study ◽  
Stable Isotope Tracer ◽  
Isotope Tracer

scholarly journals Physiologic requirement for iron in pregnant women, assessed using the stable isotope tracer technique

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Jie Cai ◽  
Tongxiang Ren ◽  
Jiaxi Lu ◽  
Jinghuan Wu ◽  
Deqian Mao ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Pregnant Women ◽  
Tracer Technique ◽  
Stable Isotope Tracer ◽  
Isotope Tracer

Neonatal Respiratory Diseases in the Newborn Infant: Novel Insights from Stable Isotope Tracer Studies

Neonatology ◽  
2016 ◽  
Vol 109 (4) ◽  
pp. 325-333 ◽  
Author(s):  
Virgilio P. Carnielli ◽  
Chiara Giorgetti ◽  
Manuela Simonato ◽  
Luca Vedovelli ◽  
Paola Cogo
Keyword(s):  
Stable Isotope ◽  
Newborn Infant ◽  
Respiratory Diseases ◽  
Tracer Studies ◽  
Stable Isotope Tracer ◽  
Isotope Tracer
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