Isolation of extracellular vesicles from small volumes of plasma using a microfluidic aqueous two-phase system

Lab on a Chip ◽  
2020 ◽  
Vol 20 (19) ◽  
pp. 3552-3559
Author(s):  
Bo Hoon Han ◽  
Sumi Kim ◽  
Geeyoon Seo ◽  
Youhee Heo ◽  
Seok Chung ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Phase System ◽  
Recovery Efficiency ◽  
High Recovery ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
System P

A microfluidic aqueous two-phase system to isolate extracellular vesicles from small volumes of sample with high recovery efficiency.

Membrane-free electroextraction using an aqueous two-phase system

RSC Advances ◽  
2014 ◽  
Vol 4 (90) ◽  
pp. 49485-49490 ◽  
Author(s):  
C. D. M. Campos ◽  
J. K. Park ◽  
P. Neužil ◽  
J. A. F. da Silva ◽  
A. Manz
Keyword(s):  
Amino Acids ◽  
Electric Field ◽  
Electrophoretic Mobility ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
System P

We present a method of continuous electroextraction of amino acids using aqueous two phase system in a microchip. The separations occur due to differences in electrophoretic mobility and solvent affinity. The results suggest the possibility of high levels of purification by controlling the electric field across the liquid barrier.

Non-inertial lift induced migration for label-free sorting of cells in a co-flowing aqueous two-phase system

The Analyst ◽  
2019 ◽  
Vol 144 (8) ◽  
pp. 2574-2583 ◽  
Author(s):  
S. Hazra ◽  
K. S. Jayaprakash ◽  
K. Pandian ◽  
A. Raj ◽  
S. K. Mitra ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Mononuclear Cells ◽  
Phase System ◽  
Label Free ◽  
Two Phase ◽  
Peripheral Blood Mononuclear ◽  
Aqueous Two Phase System ◽  
System P ◽  
Blood Mononuclear Cells ◽  
Microfluidic Technique

We present a novel label-free passive microfluidic technique for isolation of cancer cells (EpCAM+ and CD45−) from peripheral blood mononuclear cells (PBMCs) (CD45+ and EpCAM−) in aqueous two-phase system (ATPS).

scholarly journals A novel and environmentally friendly bioprocess for separation and partial purification of polysaccharides from Cordyceps sinensis mycelia by an aqueous two-phase system

RSC Advances ◽  
2017 ◽  
Vol 7 (60) ◽  
pp. 37659-37665 ◽  
Author(s):  
Zhigang Li ◽  
Ailin Chen ◽  
Zhencheng Li ◽  
Man Qu ◽  
Huayong Chen ◽  
...  
Keyword(s):  
High Efficiency ◽  
Environmentally Friendly ◽  
Cordyceps Sinensis ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
System P ◽  
Active Substances

An aqueous two-phase system comprising ethanol and salt not only could purify CS polysaccharides with high efficiency, but also simultaneously separated multiple active substances through coupling with ultrafiltration.

scholarly journals High-yield isolation of extracellular vesicles using aqueous two-phase system

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Hyunwoo Shin ◽  
Chungmin Han ◽  
Joseph M. Labuz ◽  
Jiyoon Kim ◽  
Jongmin Kim ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
High Yield ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Isolation of High-Purity Extracellular Vesicles by Extracting Proteins Using Aqueous Two-Phase System

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0129760 ◽  
Author(s):  
Jongmin Kim ◽  
Hyunwoo Shin ◽  
Jiyoon Kim ◽  
Junho Kim ◽  
Jaesung Park
Keyword(s):  
Extracellular Vesicles ◽  
High Purity ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Partitioning-dependent conversion of polyelectrolyte assemblies in an aqueous two-phase system

Soft Matter ◽  
2018 ◽  
Vol 14 (9) ◽  
pp. 1552-1558 ◽  
Author(s):  
Qingming Ma ◽  
Hao Yuan ◽  
Yang Song ◽  
Youchuang Chao ◽  
Sze Yi Mak ◽  
...  
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Ph Changes ◽  
System P ◽  
Microgel Particles

The partitioning-dependent assembly of polyelectrolytes in an aqueous-two phase system can be converted from microcapsules to microgel particles via pH changes.

Experimental study and thermodynamic modeling for purification of extracted algal lipids using an organic/aqueous two-phase system

RSC Advances ◽  
2015 ◽  
Vol 5 (2) ◽  
pp. 1153-1160 ◽  
Author(s):  
Hamed Abedini Najafabadi ◽  
Gholamreza Pazuki ◽  
Manouchehr Vossoughi
Keyword(s):  
Experimental Study ◽  
Organic Solvent ◽  
Thermodynamic Modeling ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Crude Extracts ◽  
System P ◽  
Polar Organic Solvent

Adding water and non-polar organic solvent to the crude extracts will form two-phase system and purify the extracted lipids.

Immunostaining Extracellular Vesicles Based on an Aqueous Two-Phase System: For Analysis of Tetraspanins

2021 ◽  
Vol 4 (4) ◽  
pp. 3294-3303
Author(s):  
Jingeol Lee ◽  
Yongmin Kwon ◽  
Jaehun Jung ◽  
Hyunwoo Shin ◽  
Jaesung Park
Keyword(s):  
Extracellular Vesicles ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Organic-free, versatile sessile droplet microfluidic device for chemical separation using an aqueous two-phase system

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 654-664 ◽  
Author(s):  
Matthias Hermann ◽  
Prashant Agrawal ◽  
Iris Koch ◽  
Richard Oleschuk
Keyword(s):  
Sample Preparation ◽  
Microfluidic Device ◽  
Chemical Separation ◽  
Patterned Surfaces ◽  
Phase System ◽  
Sessile Droplet ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
System P

Versatile sample preparation platform for mixing, splitting and extraction on patterned surfaces and subsequent detection by a UV/vis smartphone spectrometer.

Influence of process variables on extraction of Cefalexin in a novel biocompatible ionic liquid based-aqueous two phase system

2015 ◽  
Vol 17 (1) ◽  
pp. 655-669 ◽  
Author(s):  
Shiva Abdolrahimi ◽  
Bahram Nasernejad ◽  
Gholamreza Pazuki
Keyword(s):  
Ionic Liquid ◽  
Phase System ◽  
Process Variables ◽  
Organic Salts ◽  
Two Phase ◽  
Salting Out ◽  
Aqueous Two Phase System ◽  
System P ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Despite the fact that ionic liquid-based aqueous two phase systems (ATPSs) have been widely studied for extraction purposes, the adequacy of biodegradable organic salts as salting out agents has been left unexploited.

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