Non-inertial lift induced migration for label-free sorting of cells in a co-flowing aqueous two-phase system

The Analyst ◽  
2019 ◽  
Vol 144 (8) ◽  
pp. 2574-2583 ◽  
Author(s):  
S. Hazra ◽  
K. S. Jayaprakash ◽  
K. Pandian ◽  
A. Raj ◽  
S. K. Mitra ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Mononuclear Cells ◽  
Phase System ◽  
Label Free ◽  
Two Phase ◽  
Peripheral Blood Mononuclear ◽  
Aqueous Two Phase System ◽  
System P ◽  
Blood Mononuclear Cells ◽  
Microfluidic Technique

We present a novel label-free passive microfluidic technique for isolation of cancer cells (EpCAM+ and CD45−) from peripheral blood mononuclear cells (PBMCs) (CD45+ and EpCAM−) in aqueous two-phase system (ATPS).

Membrane-free electroextraction using an aqueous two-phase system

RSC Advances ◽  
2014 ◽  
Vol 4 (90) ◽  
pp. 49485-49490 ◽  
Author(s):  
C. D. M. Campos ◽  
J. K. Park ◽  
P. Neužil ◽  
J. A. F. da Silva ◽  
A. Manz
Keyword(s):  
Amino Acids ◽  
Electric Field ◽  
Electrophoretic Mobility ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
System P

We present a method of continuous electroextraction of amino acids using aqueous two phase system in a microchip. The separations occur due to differences in electrophoretic mobility and solvent affinity. The results suggest the possibility of high levels of purification by controlling the electric field across the liquid barrier.

scholarly journals A novel and environmentally friendly bioprocess for separation and partial purification of polysaccharides from Cordyceps sinensis mycelia by an aqueous two-phase system

RSC Advances ◽  
2017 ◽  
Vol 7 (60) ◽  
pp. 37659-37665 ◽  
Author(s):  
Zhigang Li ◽  
Ailin Chen ◽  
Zhencheng Li ◽  
Man Qu ◽  
Huayong Chen ◽  
...  
Keyword(s):  
High Efficiency ◽  
Environmentally Friendly ◽  
Cordyceps Sinensis ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
System P ◽  
Active Substances

An aqueous two-phase system comprising ethanol and salt not only could purify CS polysaccharides with high efficiency, but also simultaneously separated multiple active substances through coupling with ultrafiltration.

Shrinking, growing, and bursting: microfluidic equilibrium control of water-in-water droplets

Lab on a Chip ◽  
2016 ◽  
Vol 16 (14) ◽  
pp. 2601-2608 ◽  
Author(s):  
Byeong-Ui Moon ◽  
Dae Kun Hwang ◽  
Scott S. H. Tsai
Keyword(s):  
Phase System ◽  
Water Droplets ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Equilibrium Control ◽  
Microfluidic Technique

We demonstrate a new microfluidic technique that controls the shrinking, growing, and bursting of aqueous two phase system (ATPS) droplets.

scholarly journals Label-free enrichment of rare unconventional circulating neoplastic cells using a microfluidic dielectrophoretic sorting device

2021 ◽  
Author(s):  
Jose Montoya Mira ◽  
Ajay Sapre ◽  
Brett Walker ◽  
Jesus Bueno Alvarez ◽  
Kyle Gustafson ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Tumor Cells ◽  
Mononuclear Cells ◽  
Digital Pcr ◽  
Clinical Samples ◽  
Label Free ◽  
Circulating Biomarkers ◽  
Neoplastic Cells ◽  
Peripheral Blood Mononuclear ◽  
Potential Methods

Abstract Technologies that facilitate analyses of circulating biomarkers from blood for cancer detection are powerful tools for improving patient outcomes. Circulating biomarkers derived directly from the primary tumor have been identified including circulating tumor cells (CTCs) and circulating hybrid cells (CHCs), described to harbor phenotypes of both tumor cells and leukocytes. CHCs are present in higher numbers than CTCs which support their translational potential. Methods for isolation of CHCs do not exist and are restricted to low-throughput, time consuming, and biased methodologies. Here, we report development of a label-free dielectrophoretic (DEP) microfluidic platform facilitating enrichment of CHCs in a high-throughput and rapid fashion. Unlike other methods, depletion of healthy peripheral blood mononuclear cells (PBMCs) drives enrichment of CHCs. We analyzed DEP differential response of PBMCs and cancer cells and demonstrated up to 96.5% depletion of PBMCs resulting in 18.6-fold enrichment of cancer cells. In PBMCs from pancreatic adenocarcinoma patients, the platform enriched for neoplastic cells identified by their KRAS mutant status using droplet digital PCR from only 2 mL of whole blood with one hour of processing. Enrichment was achieved in 75% of the clinical samples analyzed, establishing this approach as a promising way to non-invasively analyze tumor cells from patients.

scholarly journals In Vitro Cytotoxic Effect of 2-(Morpholin-4-Yl)-4,5-Bis(2’’,2’’,2’’-Trinitroethoxy)-1,3,5-Triazine on Human Fibroblasts, Peripheral Blood Mononuclear Cells and Breast Cancer Cells

2021 ◽  
Author(s):  
Larisa Limareva ◽  
Pavel Iliasov ◽  
Alexander Gidaspov ◽  
Vladimir Zalomlenkov ◽  
Aleksey Sustretov ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Breast Cancer Cells ◽  
Mononuclear Cells ◽  
Human Fibroblasts ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

2-(Morpholin-4-yl)-4,5-bis(2’’,2’’,2’’-trinitroethoxy)-1,3,5-triazine having QSAR-predicted anti-tumor activity was tested for the cytotoxicity using MTT and LDH cell viability tests. The experiments were conducted using human fibroblasts, peripheral blood mononuclear cells and breast cancer cells and allowed to identify effective cytotoxic concentration ant therapeutic range of this compound. The data obtained suggest the feasibility of the further studies of the test compound as a potential anti-cancer agent.

scholarly journals Label-Free Direct Visual Analysis of Hydrolytic Enzyme Activity Using Aqueous Two-Phase System Droplet Phase Transitions

2014 ◽  
Vol 86 (8) ◽  
pp. 4052-4057 ◽  
Author(s):  
David Lai ◽  
John P. Frampton ◽  
Michael Tsuei ◽  
Albert Kao ◽  
Shuichi Takayama
Keyword(s):  
Enzyme Activity ◽  
Phase Transitions ◽  
Visual Analysis ◽  
Hydrolytic Enzyme ◽  
Phase System ◽  
Label Free ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Droplet Phase

Isolation of extracellular vesicles from small volumes of plasma using a microfluidic aqueous two-phase system

Lab on a Chip ◽  
2020 ◽  
Vol 20 (19) ◽  
pp. 3552-3559
Author(s):  
Bo Hoon Han ◽  
Sumi Kim ◽  
Geeyoon Seo ◽  
Youhee Heo ◽  
Seok Chung ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Phase System ◽  
Recovery Efficiency ◽  
High Recovery ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
System P

A microfluidic aqueous two-phase system to isolate extracellular vesicles from small volumes of sample with high recovery efficiency.

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