scholarly journals Evidence that levels of nine essential metals in post-mortem human-Alzheimer's-brain and ex vivo rat-brain tissues are unaffected by differences in post-mortem delay, age, disease staging, and brain bank location

Metallomics ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 952-962
Author(s):  
Melissa Scholefield ◽  
Stephanie J. Church ◽  
Jingshu Xu ◽  
Sarah Kassab ◽  
Natalie J. Gardiner ◽  
...  
Keyword(s):  
Rat Brain ◽  
Ex Vivo ◽  
Braak Stage ◽  
Post Mortem ◽  
Essential Metals ◽  
Brain Bank ◽  
Disease Staging ◽  
Brain Tissues

Metal findings in human Alzheimer brains are consistent despite differences in sample post-mortem delay, age, Braak stage and biobank location.

scholarly journals Effects of Alterations of Post-Mortem Delay and Other Tissue-Collection Variables on Metabolite Levels in Human and Rat Brain

Metabolites ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 438
Author(s):  
Melissa Scholefield ◽  
Stephanie J. Church ◽  
Jingshu Xu ◽  
Andrew C. Robinson ◽  
Natalie J. Gardiner ◽  
...  
Keyword(s):  
Rat Brain ◽  
Gas Chromatography Mass Spectrometry ◽  
Rat Tissues ◽  
Braak Stage ◽  
Post Mortem ◽  
Age And Gender ◽  
Braak Staging ◽  
And Gender ◽  
And Control ◽  
The Impact

The use of post-mortem human tissue is indispensable in studies investigating alterations in metabolite levels in neurodegenerative conditions such as Alzheimer’s disease (AD). However, variability between samples may have unknown effects on metabolite concentrations. The aim of this study was to characterize the impact of such variables. Cingulate gyrus was obtained from AD cases and controls, from three brain banks. Gas chromatography-mass spectrometry (GC-MS) was used to measure and compare the levels of 66 identifiable metabolites in these tissues to determine effects of tissue-collection variables. The effect of PMD was further investigated by analysis of rat brain cortex and cerebellum collected following post-mortem delays (PMDs) of zero to 72 h. Metabolite levels between cases and controls were not replicable across cohorts with variable age- and gender-matching, PMD, and control Braak staging. Analysis of rat tissues found significant effects of PMD on 31 of 63 identified metabolites over periods up to 72 h. PMD must be kept under 24 h for metabolomics analyses on brain tissues to yield replicable results. Tissues should also be well age- and gender-matched, and Braak stage in controls should be kept to a minimum in order to minimize the impact of these variables in influencing metabolite variability.

In vitro and ex vivo distribution of [3H]harmane, an endogenous β-carboline, in rat brain

2006 ◽  
Vol 50 (3) ◽  
pp. 269-276 ◽  
Author(s):  
Neil J. Anderson ◽  
Robin J. Tyacke ◽  
Stephen M. Husbands ◽  
David J. Nutt ◽  
Alan L. Hudson ◽  
...  
Keyword(s):  
Rat Brain ◽  
Ex Vivo

23. A new adult rat brain slice preparation for ex vivo NMR spectroscopy studies of stroke

1994 ◽  
Vol 52 (1) ◽  
pp. A11
Author(s):  
M.T. Espanol ◽  
L. Litt ◽  
L.-H. Chang ◽  
T.L. James ◽  
P.R. Weinstein ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Rat Brain ◽  
Brain Slice ◽  
Ex Vivo ◽  
Slice Preparation ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Spectroscopy Studies ◽  
Rat Brain Slice ◽  
Brain Slice Preparation

Nerve growth factor (NGF) content in adult rat brain tissues is several-fold higher than generally reported and is largely associated with sedimentable fractions

1996 ◽  
Vol 728 (1) ◽  
pp. 47-56 ◽  
Author(s):  
Marius C. Hoener ◽  
Eleanore Hewitt ◽  
James M. Conner ◽  
James W. Costello ◽  
Silvio Varon
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Rat Brain ◽  
Nerve Growth ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Brain Tissues

Neurochemical and Pharmacological Properties of Tianeptine, A Novel Antidepressant

1992 ◽  
Vol 160 (S15) ◽  
pp. 56-60 ◽  
Author(s):  
C. Labrid ◽  
E. Mocaër ◽  
A. Kamoun
Keyword(s):  
Rat Brain ◽  
Ex Vivo ◽  
Animal Experiments ◽  
Pyramidal Cells ◽  
Human Platelets ◽  
Clinical Findings ◽  
Tricyclic Antidepressant ◽  
Laboratory Animals ◽  
5 Ht Uptake

Tianeptine is a tricyclic antidepressant with an unusual chemical structure (a long lateral chain grafted on to a substituted dibenzothiazepin nucleus), and with biochemical and animal-behavioural properties which are strikingly different from those of classical tricyclics. Unlike the latter, which decrease serotonin (5-HT) uptake, acute and chronic tianeptine treatment enhances 5-HT uptake in rat brain and in rat and human platelets ex vivo. In vivo, tianeptine potentiates the depletion of rat brain 5-HT by 4-methyl-alpha-ethyl metatyramine and increases rat hippocampal 5-HIAA; 5-HT uptake inhibitors (e.g. fluoxetine) have opposite effects. On iontophoretic injection into CA1 pyramidal cells, tianeptine shortens the period of neuronal hypoactivity caused by GABA or 5-HT, whereas other tricyclics prolong it, and it enhances attention, learning, and memory in laboratory animals, while classical tricyclics have opposite effects. However, the relationships between these effects of tianeptine in animal experiments and their relevance to clinical findings remain to be determined.

Determination of Protopine in Rat Brain Tissues by RRLC-ESI/Q-TOF-MS Method

2014 ◽  
Vol 6 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Pei-xiang Wang ◽  
Yong-hui Li ◽  
Yan-jing Li ◽  
Ting Geng ◽  
Ming-li Li ◽  
...  
Keyword(s):  
Rat Brain ◽  
Tof Ms ◽  
Brain Tissues

scholarly journals Nonhuman primates’ tissue banks: resources for all model organism research

2021 ◽  
Author(s):  
Claire Witham ◽  
Sara Wells
Keyword(s):  
Nonhuman Primates ◽  
Ex Vivo ◽  
Model Organism ◽  
Translation Studies ◽  
Model Organisms ◽  
Laboratory Animals ◽  
Tissue Banks ◽  
Post Mortem ◽  
Wide Range ◽  
Research Programmes

AbstractBiobanks containing tissue and other biological samples from many model organisms provide easy and faster access to ex vivo resources for a wide-range of research programmes. For all laboratory animals, collecting and preserving tissue at post-mortem is an effective way of maximising the benefits of individual animals and potentially reducing the numbers required for experimentation in the future. For primate tissues, biobanks represent the scarcest of these resources but quite possibly those most valuable for preclinical and translation studies.

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