scholarly journals Deciphering the chemical instability of sphaeropsidin A under physiological conditions – degradation studies and structural elucidation of the major metabolite

2020 ◽  
Vol 18 (40) ◽  
pp. 8147-8160
Author(s):  
Alet E. van der Westhuyzen ◽  
Aude Ingels ◽  
Rémi Rosière ◽  
Karim Amighi ◽  
Lukas Oberer ◽  
...  
Keyword(s):  
Degradation Product ◽  
Structural Elucidation ◽  
Major Metabolite ◽  
Fungal Metabolite ◽  
Physiological Conditions ◽  
Chemical Instability ◽  
Degradation Studies ◽  
Major Degradation Product ◽  
Sphaeropsidin A

The degradation of the fungal metabolite sphaeropsidin A, under physiological conditions, was investigated and the structure of the major degradation product determined.

LC Stability Studies of Voriconazole and Structural Elucidation of Its Major Degradation Product

2009 ◽  
Vol 69 (S2) ◽  
pp. 115-122 ◽  
Author(s):  
Andréa I. H. Adams ◽  
Grace Gosmann ◽  
Paulo H. Schneider ◽  
Ana M. Bergold
Keyword(s):  
Degradation Product ◽  
Structural Elucidation ◽  
Stability Studies ◽  
Major Degradation Product

Actinobolin. I. Basic degradation studies and the structure of a major degradation product

1967 ◽  
Vol 8 (17) ◽  
pp. 1589-1595 ◽  
Author(s):  
Robert F. Struck ◽  
Martha C. Thorpe ◽  
W.C. Coburn ◽  
Y.Fulmer Shealy
Keyword(s):  
Degradation Product ◽  
Degradation Studies ◽  
Major Degradation Product

Glyphosate Residues in Soil and Air: An Integrated Review

2020 ◽  
Author(s):  
Evagelia Tzanetou ◽  
Helen Karasali
Keyword(s):  
Degradation Product ◽  
Critical Review ◽  
Safety Evaluation ◽  
Environmental Safety ◽  
Soil Ecosystem ◽  
Aminomethylphosphonic Acid ◽  
The Relationship ◽  
Surface And Groundwater ◽  
Major Degradation Product

Glyphosate [N-(phosphonomethyl) glycine] (GPS) is currently the most commonly applied herbicide worldwide. Given the widespread use of glyphosate, the investigation of the relationship between glyphosate and soil ecosystem is critical and has great significance for its valid application and environmental safety evaluation. However, although the occurrence of glyphosate residues in surface and groundwater is rather well documented, only few information are available for soils and even fewer for air. Due to this, the importance of developing methods that are effective and fast to determine and quantify glyphosate and its major degradation product, aminomethylphosphonic acid (AMPA), is emphasized. Based on its structure, the determination of this pesticide using a simple analytical method remains a challenge, a fact known as the “glyphosate paradox.” In this chapter a critical review of the existing literature and data comparison studies regarding the occurrence and the development of analytical methods for the determination of pesticide glyphosate in soil and air is performed.

Kinetic Degradation Study of Dapagliflozin Coupled with UHPLC Separation in the Presence of Major Degradation Product and Metformin

2019 ◽  
Vol 82 (4) ◽  
pp. 777-789 ◽  
Author(s):  
Wafaa A. Zaghary ◽  
Shereen Mowaka ◽  
Moataz S. Hendy
Keyword(s):  
Degradation Product ◽  
Degradation Study ◽  
Major Degradation Product

Confirmation of the structure of nisin and its major degradation product by FAB-MS and FAB-MS/MS

1988 ◽  
Vol 44 (3) ◽  
pp. 266-270 ◽  
Author(s):  
M. Barber ◽  
G. J. Elliot ◽  
R. S. Bordoli ◽  
B. N. Green ◽  
B. W. Bycroft
Keyword(s):  
Degradation Product ◽  
Major Degradation Product

Structural elucidation of novel degradation product of brotizolam

2013 ◽  
Vol 23 (12) ◽  
pp. 3515-3518 ◽  
Author(s):  
Yoshinori Sugimoto ◽  
Aya Kugimiya ◽  
Yoshifumi Yoshimura ◽  
Hideo Naoki
Keyword(s):  
Degradation Product ◽  
Structural Elucidation

scholarly journals FORCED DEGRADATION STUDIES OF NILOTINIB HYDROCHLORIDE: ISOLATION, IDENTIFICATION & CHARACTERIZATION OF IMPURITIES

2020 ◽  
pp. 537-543
Author(s):  
JCMKNN Murty Singamsetti ◽  
Raghu Babu Korupolu ◽  
Himabindhu Gandham ◽  
Mahesh Kumar Reddy Geereddi ◽  
Muralidharan Kaliyaperumal ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Degradation Product ◽  
Kinase Inhibitor ◽  
Degradation Products ◽  
Myelogenous Leukemia ◽  
Forced Degradation ◽  
Molecular Formula ◽  
Forced Degradation Studies ◽  
Acidic Environments ◽  
Degradation Studies

Nilotinib hydrochloride is a tyrosine kinase inhibitor approved for the treatment of chronic myelogenous leukemia was subjected to forced degradation studies and the samples were analyzed by utilizing the LCMS compatible HPLC methods. Nilotinib Hydrochloride was subjected to thermal, hydrolytic, oxidative, acidic, basic and photolytic degradation conditions as per the regulatory guidelines. The drug was degraded in oxidative, basic and acidic environments and stable in photolytic and thermal conditions. The main degradation impurity components produced through the forced degradation study were isolated for the identification and quantification in presence of these impurities in the stability studies of drug substances as well as drug products. The identified degradation components were separated by mass assisted auto-purification technique and subjected for the characterization by NMR (13C-NMR, 1H-NMR, HMBC and HSQC), HRMS and FT-IR experimentations. Degradation products obtained from oxidative, basic and acidic environments were isolated and identified by the advanced techniques  as acid degradation product (DP-1) with molecular mass of 306.11 g/mol, empirical formula C17H14N4O2 with name as 4-methyl-3- (4 -(pyridine -3-yl) pyrimidin -2 -ylamino) benzoic acid. Base degradation product (DP-2) has molecular weight of 241.08 g/mol, molecular formula C11H10F3N3 with name as 3-(4-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)aniline.Oxidative degradation product (DP-3) has molecular weight of 545.18 g/mol, molecular formula C28H22F3N7O2 with name as 3-(2-(2-methyl-5-(3-(4-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)phenylcarbamoyl) phenylamino)pyrimidin-4-yl)pyridine1-oxide.  

Like Fibrin, (DD)E, the Major Degradation Product of Crosslinked Fibrin, Protects Plasmin from Inhibition by α2-antiplasmin

2001 ◽  
Vol 85 (03) ◽  
pp. 502-508 ◽  
Author(s):  
Agnes Lee ◽  
James Fredenburgh ◽  
Ronald Stewart ◽  
Janice Rischke ◽  
Jeffrey Weitz
Keyword(s):  
Protective Effect ◽  
Plasminogen Activator ◽  
Degradation Product ◽  
Tissue Type ◽  
Plasminogen Activation ◽  
Tissue Type Plasminogen Activator ◽  
Potent Stimulator ◽  
Type Plasminogen Activator ◽  
Kd Values ◽  
Major Degradation Product

SummaryPlasmin generation is localized to the fibrin surface because tissue-type plasminogen activator (t-PA) and plasminogen bind to fibrin, an interaction that stimulates plasminogen activation over a hundred-fold. To ensure efficient fibrinolysis, plasmin bound to fibrin is protected from inhibition by α2-antiplasmin. (DD)E, a major soluble degradation product of cross-linked fibrin that is a potent stimulator of t-PA, compromises the fibrin-specificity of t-PA by promoting systemic activation of plasminogen. In this study we investigated whether (DD)E also protects plasmin from inhibition by α2-antiplasmin, facilitating degradation of this soluble t-PA effector. (DD)E and fibrin reduce the rate of plasmin inhibition by α2-antiplasmin by 5- and 10-fold, respectively. Kringle-dependent binding of plasmin to (DD)E and fibrin, with Kd values of 52 and 410 nM, respectively, contributes to the protective effect. When (DD)E is extensively degraded by plasmin, yielding uncomplexed fragment E and (DD), protection of plasmin from inhibition by α2-antiplasmin is attenuated. These studies indicate that (DD)E-bound plasmin, whose generation reflects the ability of (DD)E to stimulate plasminogen activation by t-PA, has the capacity to degrade (DD)E by virtue of its resistance to inhibition. This provides a mechanism to limit the concentration of (DD)E and maintain the fibrin-specificity of t-PA.

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