A Non‐Enzymatic Disposable Electrochemical Sensor Based on Surface-Modified Screen-Printed Electrodes CuO-IL/rGO Nanocomposite for Single-Step Determination of Glucose in Human Urine and Electrolyte Drink

2021 ◽  
Author(s):  
Nopparat Janmee ◽  
Pattarachaya Preechakasedkit ◽  
Nadnudda Rodthongkum ◽  
Orawon Chailapakul ◽  
Pranut Potiyaraj ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Electrochemical Sensor ◽  
Human Urine ◽  
Sample Pretreatment ◽  
Single Step ◽  
Glucose Determination ◽  
Preparation Step ◽  
Surface Modified ◽  
Additional Sample

A non‐enzymatic disposable electrochemical sensor coupled with an automated sample pretreatment paper-based device was developed to avoid an additional sample preparation step for glucose determination in human urine and electrolyte...

scholarly journals Simultaneous determination of iron and copper in children's sera by FAAS

2011 ◽  
Vol 61 (1) ◽  
pp. 93-102 ◽  
Author(s):  
Svjetlana Luterotti ◽  
Tončica Kordić ◽  
Slavica Dodig
Keyword(s):  
Atomic Absorption ◽  
Simultaneous Determination ◽  
Sample Pretreatment ◽  
Single Step ◽  
Design Experiment ◽  
New Approach ◽  
Flame Atomic Absorption ◽  
Reference Methods ◽  
Atomic Absorption Spectrometric

Simultaneous determination of iron and copper in children's sera by FAASA new and simple flame atomic-absorption spectrometric (FAAS) method is proposed for simultaneous determination of iron and copper in children's sera. It is based on single-step sample pretreatment (deproteinization with 3 mol L-1HCl, ratio 1:1) and single-step calibration using 1.5 mol L-1HCl standard. During method's optimization a short multifactorial design experiment was used. The proposed method assures accuracy, sensitivity and precision comparable to that of the reference methods. The new approach is simple and time-, labour- and serum-saving, the latter being especially important in pediatric diagnostics.

The Role of Resolution and Sample Preparation in Hydration Rim Measurement: Implications for Experimentally Determined Hydration Rates

1988 ◽  
Vol 53 (1) ◽  
pp. 110-117 ◽  
Author(s):  
Barry E. Scheetz ◽  
Christopher M. Stevenson
Keyword(s):  
Activation Energy ◽  
Sample Preparation ◽  
Error Analysis ◽  
Single Step ◽  
Theoretical Limit

The role of the theoretical limit of resolution of optical microscopes is discussed in relation to obsidian rim age-date determinations. The resolution of the optics is shown to contribute an error of ± 20 percent. The error analysis identified the determination of the activation energy as the single step in the dating process that introduces the largest error.

scholarly journals UPLC-MS/MS method for determination of retinol and α-tocopherol in serum using a simple sample pretreatment and UniSpray as ionization technique to reduce matrix effects

2020 ◽  
Vol 58 (5) ◽  
pp. 769-779 ◽  
Author(s):  
Nele Peersman ◽  
Jan Van Elslande ◽  
Yannick Lepage ◽  
Samira De Amicis ◽  
Koenraad Desmet ◽  
...  
Keyword(s):  
Matrix Effects ◽  
Solid Phase ◽  
Sample Pretreatment ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Single Step ◽  
Limit Of Quantification ◽  
Phase Gradient ◽  
Ionization Technique

AbstractBackgroundOur goal was to develop a simple, rapid and precise ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the determination of retinol and α-tocopherol in serum. Currently published LC-MS/MS methods either require complex extraction procedures (liquid-liquid or solid-phase) or do not meet desirable specifications for imprecision in serum (coefficient of variation [CV] <6.8% and 6.9%, respectively).MethodsSample preparation consisted of a simple protein precipitation with ethanol and acetonitrile. Stable isotope-labeled internal standards (IS) and a homemade calibration curve were used for quantification. The analysis was performed using an Acquity I-class Xevo TQ XS LC-MS/MS. Chromatographic runtime was 6.0 min using a reversed phase gradient elution. UniSpray (US) as an ionization technique was compared to electrospray ionization (ESI). Analytical validation included matrix effect, recovery and trueness compared to National Institute of Standards and Technology (NIST) standards and United Kingdom National External Quality Assessment Service (UK NEQAS) samples.ResultsIntra- and inter-run CVs were <4.9% for retinol and <1.7% for α-tocopherol, both complying with desirable specifications for imprecision. Bias compared to NIST standards was <3.1% for both compounds. The method was linear over the entire tested range. The lower limit of quantification (LLOQ) with US was lower than with ESI for both retinol (0.022 vs. 0.043 mg/L) and α-tocopherol (0.22 vs. 0.87 mg/L). Matrix effects were not significant (<15%) for retinol. However, for α-tocopherol matrix effects of on average 54.0% were noted using ESI, but not with US.ConclusionsWe developed a fast, precise and accurate UPLC-MS/MS method for the determination of retinol and α-tocopherol in human serum using a single-step sample pretreatment. Ionization using US eliminated the matrix effects for α-tocopherol.

scholarly journals HPLC method for the simultaneous determination of four compounds in rat plasma after intravenous administration of Portulaca oleracea L. extract

2012 ◽  
Vol 48 (1) ◽  
pp. 163-170 ◽  
Author(s):  
Zhongzhe Cheng ◽  
Ming Xie ◽  
Wenjie Zhang ◽  
Lan Cheng ◽  
Yang Du ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Intravenous Administration ◽  
Internal Standard ◽  
Sample Pretreatment ◽  
Rat Plasma ◽  
Hplc Method ◽  
Single Step ◽  
Portulaca Oleracea ◽  
Coumaric Acid

The objective of the present study was to develop a simple and selective HPLC method for the simultaneous determination of hesperidin (HP), caffeic acid (CA), ferulic acid (FA) and p-coumaric acid (p-CA) in rat plasma after intravenous administration of Portulaca oleracea L. extract (POE). With the hyperoside as the internal standard, the sample pretreatment procedure involved simple single-step extraction with methanol of 0.2 mL plasma. The mobile phase consisted of methanol-acetonitrile-tetrahydrofuran-0.5% glacial acetic acid (5:3:18:74, v/v/v/v). The calibration curves were linear over the range of 0.1-25 µg mL-1, 0.1-25 µg mL-1, 0.1-25 µg mL-1and 0.015-3 µg mL-1 for HP, CA, FA and p-CA, respectively. The method developed was suitable for the pharmacokinetic study of HP, CA, FA and p-CA in rats after intravenous administration of POE.

New voltammetric analysis of olanzapine in tablets and human urine samples using a modified carbon paste sensor electrode incorporating gold nanoparticles and glutamine in a micellar medium

2015 ◽  
Vol 7 (2) ◽  
pp. 581-589 ◽  
Author(s):  
Hytham M. Ahmed ◽  
Mona A. Mohamed ◽  
Waheed M. Salem
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Sensor ◽  
Human Urine ◽  
Urine Samples ◽  
Micellar Medium ◽  
Carbon Paste ◽  
Voltammetric Analysis ◽  
Selective Determination ◽  
Sensor Electrode

An effective novel electrochemical sensor for the selective determination of olanzapine (OLA) was introduced.

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