Template-free multiple signal amplification for highly sensitive detection of cancer cell-derived exosomes

2021 ◽  
Author(s):  
Lei Wang ◽  
Ying Deng ◽  
Yue Huang ◽  
Juan Wei ◽  
Jiehua Ma ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Multiple Signal ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Template Free ◽  
Highly Sensitive Detection

Herein, we have designed a template-free multiple signal amplification method in this work for the detection of cancer cell-derived exosomes with high sensitivity. In this design, the combined utilization of...

Carbon nanoparticle-protected aptamers for highly sensitive and selective detection of biomolecules based on nuclease-assisted target recycling signal amplification

2014 ◽  
Vol 50 (57) ◽  
pp. 7646-7648 ◽  
Author(s):  
Xiaoyan Lin ◽  
Liang Cui ◽  
Yishun Huang ◽  
Ya Lin ◽  
Yi Xie ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Carbon Nanoparticles ◽  
Sensitive Detection ◽  
Selective Detection ◽  
Carbon Nanoparticle ◽  
Target Recycling ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Detection Of Biomolecules ◽  
Highly Sensitive Detection

A nuclease-assisted target recycling signal amplification method based on carbon nanoparticles for highly sensitive detection of biomolecules was developed.

An electrochemical immunosensor based on a multiple signal amplification strategy for highly sensitive detection of prostate specific antigen

2018 ◽  
Vol 10 (40) ◽  
pp. 4917-4925 ◽  
Author(s):  
Li Liu ◽  
Guanhui Zhao ◽  
Xue Dong ◽  
Xuan Li ◽  
Qin Wei ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Signal Amplification ◽  
Specific Antigen ◽  
Electrochemical Immunosensor ◽  
Sensitive Detection ◽  
Multiple Signal ◽  
Highly Sensitive ◽  
Sandwich Type ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

A sandwich-type electrochemical immunosensor was developed based on a multiple signal amplification strategy.

scholarly journals RAP: A Novel Approach to the Rapid and Highly Sensitive Detection of Respiratory Viruses

2021 ◽  
Vol 9 ◽  
Author(s):  
Guohao Fan ◽  
Ruiqing Zhang ◽  
Xiaozhou He ◽  
Fengyu Tian ◽  
Mingzhu Nie ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Human Adenovirus ◽  
Respiratory Viruses ◽  
Sensitive Detection ◽  
Taqman Probe ◽  
Amplification Method ◽  
Novel Approach ◽  
Highly Sensitive ◽  
Amplification Process ◽  
Highly Sensitive Detection

Recombinase aided amplification (RAA) is an emerging isothermal amplification method used for detecting various pathogens. However, RAA requires a complex and long probe to ensure high sensitivity during fluorescence assay. TaqMan probe used for quantitative PCR (qPCR) is simple and universal. Herein, we developed a new approach for detecting nucleic acids of pathogens, known as RAP (Recombinase aided PCR). The method combines RAA and qPCR to ensure a rapid and highly sensitive detection using a conventional qPCR device. RAP is a two-stage amplification process performed in a single tube within 1 hour. The method involves an RAA reaction for 10 min at 39°C (first stage) followed by 15 cycles of qPCR (second stage). Using human adenovirus 3 (HADV3) and human adenovirus 7 (HADV7) plasmids, the sensitivities of RAP assays for detecting HADV3 and HADV7 were 6 and 17 copies per reaction, respectively. The limit of RAP detection was at least 16-fold lower than the corresponding qPCR, and no-cross reaction with other respiratory viruses was observed. The results of RAP analysis revealed 100% consistency with qPCR assay. This study shows that RAP assay is a rapid, specific, and highly sensitive detection method with a potential for clinical and laboratory application.

scholarly journals Highly sensitive detection of nitrobenzene by a series of fluorescent 2D zinc(ii) metal–organic frameworks with a flexible triangular ligand

RSC Advances ◽  
2021 ◽  
Vol 11 (39) ◽  
pp. 23975-23984
Author(s):  
Xue Yang ◽  
Yixia Ren ◽  
Hongmei Chai ◽  
Xiufang Hou ◽  
Zhixiang Wang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Metal Organic Frameworks ◽  
High Sensitivity ◽  
Fluorescent Sensors ◽  
Sensitive Detection ◽  
Donor Ligands ◽  
Highly Sensitive ◽  
Metal Organic ◽  
Highly Sensitive Detection

Four fluorescent 2D Zn-MOFs based on a flexible triangular ligand and linear N-donor ligands are hydrothermally prepared and used to detect nitrobenzene in aqueous solution with high sensitivity, demonstrating their potential as fluorescent sensors.

Highly sensitive detection of Cr(vi) in groundwater by bimetallic NiFe nanoparticles

2017 ◽  
Vol 9 (6) ◽  
pp. 1031-1037 ◽  
Author(s):  
Jingtao Liu ◽  
Yu Ding ◽  
Lifei Ji ◽  
Xin Zhang ◽  
Fengchun Yang ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Hexavalent Chromium ◽  
Low Cost ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Toxic Heavy Metal ◽  
Metal Pollutants ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Heavy Metal Pollutants

Hexavalent chromium (Cr(vi)) is one of the most toxic heavy metal pollutants in groundwater, and thus the detection of Cr(vi) with high sensitivity, accuracy, and simplicity and low cost is of great importance.

A highly sensitive fluorescence biosensor for detection of Staphylococcus aureus based on HCR-mediated three-way DNA junction nicking enzyme assisted signal amplification

The Analyst ◽  
2021 ◽  
Author(s):  
Chuyan Zhang ◽  
Zewei Luo ◽  
Mengfan Wu ◽  
Wei Ning ◽  
Ziyi Tian ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Food Safety ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Safety Control ◽  
Food Borne ◽  
Highly Sensitive ◽  
Fluorescence Biosensor ◽  
Highly Sensitive Detection ◽  
Nicking Enzyme

Sensitive and efficient monitoring of food-borne bacteria is of great importance for food safety control. Herein, a novel biosensor for highly sensitive detection of Staphylococcus aureus (S. aureus) was constructed...

scholarly journals Development of micropillar array electrodes for highly sensitive detection of biomarkers

RSC Advances ◽  
2020 ◽  
Vol 10 (67) ◽  
pp. 41110-41119
Author(s):  
Chaozhan Chen ◽  
Bin Ran ◽  
Zhenxing Wang ◽  
Hongli Zhao ◽  
Minbo Lan ◽  
...  
Keyword(s):  
Surface Modification ◽  
Surface Area ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Detection Of Biomarkers ◽  
Highly Sensitive Detection ◽  
Micropillar Array

PDMS-based micropillar array electrodes with increased surface area and surface modification were developed to detect biomarkers with high sensitivity.

A QCM Immunosensor for Rapid Determination of Ractopamine in Food Based on Enzyme-AuNPs for Signal Amplification and Magnetic β-Cyclodextrins for Extraction

2015 ◽  
Vol 738-739 ◽  
pp. 56-60
Author(s):  
Shu Xian Chen ◽  
Jing Liu ◽  
Dao Dong Pan ◽  
Ning Gan
Keyword(s):  
Signal Amplification ◽  
Rapid Determination ◽  
Screening Method ◽  
High Sensitivity ◽  
The Novel ◽  
Highly Sensitive ◽  
Reliable Quantification ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

An enzyme amplified immunosensor for highly sensitive detection of Ractopamine (RAC) in foodstuff was developed based on quartz crystal microbalance (QCM). The high sensitivity was achieved by enzyme-AuNPs signal amplification strategy and magnetic β-cyclodextrins (β-CD) enrichment capacity. The novel QCM immunosensor which combines with the advantages of high selectivity of immunoassays and the high sensitivity of QCM has been developed for the determination of trace residues of RAC in food production. Under optimum conditions, the differences in the frequencies (∆f) of the QCM were proportional to the concentration of RAC over the range from 0.01 to 10 ng mL-1. The minimal detection limit was 0.01 ng mL-1. Due to its high sensitivity, acceptable stability and good selectivity, the immunosensor realized reliable quantification of RAC in real foodstuff. The proposed project has the potential to become a successful on-site screening method in food safety.

scholarly journals High-sensitivity small-molecule detection of microcystin-LR cyano-toxin using a terahertz-aptamer biosensor

The Analyst ◽  
2021 ◽  
Author(s):  
Ahmed Mohamed ◽  
Ryan Walsh ◽  
Mohamed Cherif ◽  
Hassan A. Hafez ◽  
Xavier Ropagnol ◽  
...  
Keyword(s):  
Small Molecule ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Small Molecule Detection

We demonstrate the rapid and highly sensitive detection of a small molecule, microcystin-LR (MC-LR) toxin using an aptasensor based on a terahertz (THz) emission technique named the terahertz chemical microscope (TCM).

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