scholarly journals Recent advances in fluorescent probes for lipid droplets

2022 ◽  
Author(s):  
Yanyan Zhao ◽  
Wen Shi ◽  
Xiaohua Li ◽  
Huimin Ma
Keyword(s):  
Fluorescent Probes ◽  
Lipid Droplets ◽  
Lipid Storage ◽  
Cellular Organelle ◽  
Recent Advances ◽  
In Cells

Lipid droplets (LDs) have been known as a non-negligible cellular organelle for lipid storage and metabolism. Fluorescent probes for imaging LDs would be paramount for depicting their functions in cells....

scholarly journals Recent Advances in Fluorescent Probes for Lipid Droplets

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1768 ◽  
Author(s):  
Tkhe Fam ◽  
Andrey Klymchenko ◽  
Mayeul Collot
Keyword(s):  
Fluorescent Probes ◽  
Lipid Droplets ◽  
Neutral Lipids ◽  
Photophysical Properties ◽  
Tissue Imaging ◽  
Two Photon ◽  
Physiological Processes ◽  
Recent Advances ◽  
Photon Excitation

Lipid droplets (LDs) are organelles that serve as the storage of intracellular neutral lipids. LDs regulate many physiological processes. They recently attracted attention after extensive studies showed their involvement in metabolic disorders and diseases such as obesity, diabetes, and cancer. Therefore, it is of the highest importance to have reliable imaging tools. In this review, we focus on recent advances in the development of selective fluorescent probes for LDs. Their photophysical properties are described, and their advantages and drawbacks in fluorescence imaging are discussed. At last, we review the reported applications using these probes including two-photon excitation, in vivo and tissue imaging, as well as LDs tracking.

Recent advances in quinazolinones as an emerging molecular platform for luminescent materials and bioimaging

2021 ◽  
Vol 8 (8) ◽  
pp. 1867-1889
Author(s):  
Zhiming Xing ◽  
Wanhui Wu ◽  
Yongxiang Miao ◽  
Yingqun Tang ◽  
Youkang Zhou ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Biological Imaging ◽  
Luminescence Properties ◽  
Luminescent Materials ◽  
Future Outlook ◽  
Recent Advances

This review summarized recent advances relating to the luminescence properties of quinazolinones and their applications in fluorescent probes, biological imaging and luminescent materials. Their future outlook is also included.

scholarly journals Small-molecule fluorescence-based probes for interrogating major organ diseases

2021 ◽  
Author(s):  
Hai-Hao Han ◽  
He Tian ◽  
Yi Zang ◽  
Adam C. Sedgwick ◽  
Jia Li ◽  
...  
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Recent Advances ◽  
Major Organ

This tutorial review describes recent advances involving small molecule fluorescent probes designed to aid in the study of major organ diseases.

scholarly journals A Bimodal Fluorescence-Raman Probe for Cellular Imaging

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1699
Author(s):  
Jiarun Lin ◽  
Marcus E. Graziotto ◽  
Peter A. Lay ◽  
Elizabeth J. New
Keyword(s):  
Chemical Composition ◽  
Fluorescent Probes ◽  
Lipid Droplets ◽  
Imaging Techniques ◽  
Spectroscopic Imaging ◽  
Fluorescent Sensors ◽  
Chemical Environment ◽  
Novel Approach ◽  
Health And Disease ◽  
Raman Probe

Biochemical changes in specific organelles underpin cellular function, and studying these changes is crucial to understand health and disease. Fluorescent probes have become important biosensing and imaging tools as they can be targeted to specific organelles and can detect changes in their chemical environment. However, the sensing capacity of fluorescent probes is highly specific and is often limited to a single analyte of interest. A novel approach to imaging organelles is to combine fluorescent sensors with vibrational spectroscopic imaging techniques; the latter provides a comprehensive map of the relative biochemical distributions throughout the cell to gain a more complete picture of the biochemistry of organelles. We have developed NpCN1, a bimodal fluorescence-Raman probe targeted to the lipid droplets, incorporating a nitrile as a Raman tag. NpCN1 was successfully used to image lipid droplets in 3T3-L1 cells in both fluorescence and Raman modalities, reporting on the chemical composition and distribution of the lipid droplets in the cells.

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