Exploration of benign deep eutectic solvent–water system for the high-efficient production of furfurylamine from sugarcane bagasse via chemoenzymatic cascade catalysis

2021 ◽  
Author(s):  
Qing Li ◽  
Junhua Di ◽  
Xiaolong Liao ◽  
Jiacheng Ni ◽  
Qi Li ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Deep Eutectic Solvent ◽  
Reaction System ◽  
Water System ◽  
Value Added ◽  
Environmentally Friendly ◽  
Cost Effective ◽  
Efficient Production ◽  
Solvent Water ◽  
High Efficient

Recently, cost-effective production of highly value-added furan chemicals from abundant and renewable bioresources has gained much attentions via chemoenzymatic approach in environmentally-friendly reaction system. In this work, chemoenzymatic cascade reaction...

scholarly journals Chemoenzymatic Conversion of Biomass-Derived D-Xylose to Furfuryl Alcohol with Corn Stalk-Based Solid Acid Catalyst and Reductase Biocatalyst in a Deep Eutectic Solvent–Water System

Processes ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 113
Author(s):  
Jianguang Liang ◽  
Li Ji ◽  
Jiarui He ◽  
Shuxin Tang ◽  
Yucai He
Keyword(s):  
Furfuryl Alcohol ◽  
Solid Acid ◽  
Solid Acid Catalyst ◽  
Deep Eutectic Solvent ◽  
Water System ◽  
Value Added ◽  
Acid Catalyst ◽  
Corn Stalk ◽  
Solvent Water ◽  
Water Media

In this work, the feasibility of chemoenzymatically transforming biomass-derived D-xylose to furfuryl alcohol was demonstrated in a tandem reaction with SO42−/SnO2-CS chemocatalyst and reductase biocatalyst in the deep eutectic solvent (DES)–water media. The high furfural yield (44.6%) was obtained by catalyzing biomass-derived D-xylose (75.0 g/L) in 20 min at 185 °C with SO42−/SnO2-CS (1.2 wt%) in DES ChCl:EG–water (5:95, v/v). Subsequently, recombinant E.coli CF cells harboring reductases transformed D-xylose-derived furfural (200.0 mM) to furfuryl alcohol in the yield of 35.7% (based on D-xylose) at 35 °C and pH 7.5 using HCOONa as cosubstrate in ChCl:EG–water. This chemoenzymatic cascade catalysis strategy could be employed for the sustainable production of value-added furan-based chemical from renewable bioresource.

scholarly journals Optimization of Metal Ions in Sugarcane Bagasse Fermenting Medium for the Production of Streptokinase by Streptococcus equisimilis

2021 ◽  
Vol 9 (2) ◽  
pp. 24-30
Keyword(s):  
Metal Ions ◽  
Sugarcane Bagasse ◽  
Specific Activity ◽  
Gel Filtration ◽  
Value Added ◽  
Cost Effective ◽  
Fermentation Medium ◽  
Exchange Chromatography ◽  
Sds Page ◽  
The Cost

Streptokinase is a fibrinolytic enzyme and a product of β-hemolytic Streptococci strains. This enzyme is used as a medication to break down clots in some cases of heart disease. Streptococcus equisimilis, a species of group C Streptococci, is widely used for the production of streptokinase by fermentation technology. In this study, the sugarcane bagasse fermentation medium was optimized for metal ions (KH2PO4, MgSO4.7H2O, CaCO3 and NaHCO3) at various levels to attain the maximal production of streptokinase. Sugarcane bagasse was used due to its profuse availability and as an ideal substrate for microbial processes for the manufacturing of value-added products. The results showed that maximal streptokinase production was found at 0.04% KH2PO4, 0.04% MgSO4.7H2O, 0.15% NaHCO3 and 0.04% CaCO3. Finally, the optimized medium resulted in 84.75 U/mg specific activity and 74.5% recovery. The purification process was carried out simultaneously using ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration. Finally, a purified sample of streptokinase was run on SDS-PAGE and resolute 47 kDa molecular weight. The use of β-hemolytic Streptococci to obtain streptokinase is not free from health risks and is related to anaphylaxis. This study provides a way forward for the cost-effective ways to obtain streptokinase for the treatment of thrombosis.

scholarly journals High-Efficient Production of (S)-1-[3,5-Bis(trifluoromethyl)phenyl]ethanol via Whole-Cell Catalyst in Deep-Eutectic Solvent-Containing Micro-Aerobic Medium System

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1855
Author(s):  
Zhiren Zhu ◽  
Shunde Bi ◽  
Ning Ye ◽  
Pu Wang
Keyword(s):  
Deep Eutectic Solvent ◽  
Reaction System ◽  
Cell Membrane Permeability ◽  
Respiratory Pattern ◽  
Attractive Potential ◽  
Valuable Insight ◽  
Whole Cell ◽  
Dry Cell Weight ◽  
High Efficient ◽  
Whole Cell Biocatalyst

The ratio of substrate to catalyst (S/C) is a prime target for the application of asymmetric production of enantiomerically enriched intermediates by whole-cell biocatalyst. In the present study, an attractive increase in S/C was achieved in a natural deep-eutectic solvent (NADES) containing reaction system under microaerobic condition for high production of (S)-1-[3,5-bis(trifluoromethyl)phenyl]ethanol ((S)-3,5-BTPE) with Candida tropicalis 104. In PBS buffer (0.2 M, pH 8.0) at 200 rpm and 30 °C, 79.5 g (Dry Cell Weight, DCW)/L C. tropicalis 104 maintained the same yield of 73.7% for the bioreduction of 3,5-bis(trifluoromethyl)acetophenone (BTAP) under an oxygen-deficient environment compared with oxygen-sufficient conditions, while substrate load increased 4.0-fold (from 50 mM to 200 mM). Furthermore, when choline chloride:trehalose (ChCl:T, 1:1 molar ratio) was introduced into the reaction system for its versatility of increasing cell membrane permeability and declining BTAP cytotoxicity to biocatalyst, the yields were further increased to 86.2% under 200 mM BTAP, or 72.9% at 300 mM BTAP. After the optimization of various reaction parameters involved in the bioreduction, and the amount of biocatalyst and maltose co-substrate remained 79.5 g (DCW)/L and 50 g/L, the S/C for the reduction elevated 6.3 times (3.8 mM/g versus 0.6 mM/g). By altering the respiratory pattern of the whole-cell biocatalyst and exploiting the ChCl:T-containing reaction system, the developed strategy exhibits an attractive potential for enhancing catalytic efficiency of whole-cell-mediated reduction, and provides valuable insight for the development of whole-cell catalysis.

Molar Enthalpy of Mixing for Choline Chloride/Urea Deep Eutectic Solvent + Water System

2016 ◽  
Vol 61 (12) ◽  
pp. 4172-4177 ◽  
Author(s):  
Chunyan Ma ◽  
Yanhua Guo ◽  
Dongxue Li ◽  
Jianpeng Zong ◽  
Xiaoyan Ji ◽  
...  
Keyword(s):  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Water System ◽  
Enthalpy Of Mixing ◽  
Molar Enthalpy ◽  
Solvent Water

Boosting photocatalytic performance for selective oxidation of biomass-derived pentoses and hexoses to lactic acid using hierarchically porous Cu/Cu2O/CuO@CA

2021 ◽  
Author(s):  
Dongnv Jin ◽  
Gaojie Jiao ◽  
Wenfeng Ren ◽  
Jinghui Zhou ◽  
Jiliang Ma ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Selective Oxidation ◽  
Photocatalytic Performance ◽  
Value Added ◽  
Environmentally Friendly ◽  
Cost Effective ◽  
Hierarchically Porous ◽  
Limited Success

Photocatalytic-reforming of biomass-derived feedstocks to high-value added chemicals is highly desired, yet limited success has been achieved. To this aim, the development of an efficient, cost-effective, and environmentally friendly photocatalyst...

Functional assessment of biodegradable cotton nonwoven substrates permeated with spatial insect repellants for disposable applications

2021 ◽  
pp. 004051752098721
Author(s):  
Rebecca J. Hron ◽  
Doug J. Hinchliffe ◽  
Michael Santiago Cintrón ◽  
Kenneth J. Linthicum ◽  
Brian D. Condon
Keyword(s):  
Value Added ◽  
Environmentally Friendly ◽  
Cost Effective ◽  
Array Detector ◽  
Scanning Calorimetry ◽  
Modulated Differential Scanning Calorimetry ◽  
Insect Repellents ◽  
Nonwoven Fabrics ◽  
Preventative Measures ◽  
Spatial Repellents

Arboviral diseases and malaria transmitted by mosquitoes are major health concerns worldwide responsible for millions of deaths annually. Spatial repellents have been used as preventative measures via direct application to the skin or disposable devices worn outside of clothing. Naturally derived, plant-based repellents are safe, effective alternatives to synthetic repellents and may be applied to disposable substrates including adhesive patches. Many disposables are composed of synthetic petrochemical-based polyester and polypropylene nonwoven fabrics, contributing to growing concern surrounding microplastic pollution. Sustainable and biodegradable substrates including those made from cotton are cost effective and environmentally friendly. Therefore, we explored the utilization of cotton-based substrates for the application of natural spatial and synthetic contact insect repellents. Cotton fibers used in the study were commercially available greige cotton, scoured and bleached greige cotton and reginned greige cotton motes, a value-added byproduct of the ginning process. Synthetic polyester and polypropylene were used for comparison. Thermogravimetric/derivative thermogravimetric analysis (TG/DTG) in combination with modulated differential scanning calorimetry (mDSC) were used to discern vaporization properties of repellents from the substrates. Retention times of repellents on the substrates were analyzed using a Fourier-transform infrared spectroscopy–focal plane array detector and compared with the TG/DTG and mDSC data. Mosquito landing assays confirmed the feasibility of using cotton-based substrates treated with plant-based spatial repellents as an effective and environmentally friendly alternative to synthetic materials.

scholarly journals Optimization of Metal Ions in Sugarcane Bagasse Fermenting Medium for the Production of Streptokinase by Streptococcus equisimilis

2021 ◽  
Vol 9 (2) ◽  
Keyword(s):  
Metal Ions ◽  
Sugarcane Bagasse ◽  
Specific Activity ◽  
Gel Filtration ◽  
Value Added ◽  
Cost Effective ◽  
Fermentation Medium ◽  
Exchange Chromatography ◽  
Sds Page ◽  
The Cost

Streptokinase is a fibrinolytic enzyme and a product of β-hemolytic Streptococci strains. This enzyme is used as a medication to break down clots in some cases of heart disease. Streptococcus equisimilis, a species of group C Streptococci, is widely used for the production of streptokinase by fermentation technology. In this study, the sugarcane bagasse fermentation medium was optimized for metal ions (KH2PO4, MgSO4.7H2O, CaCO3 and NaHCO3) at various levels to attain the maximal production of streptokinase. Sugarcane bagasse was used due to its profuse availability and as an ideal substrate for microbial processes for the manufacturing of value-added products. The results showed that maximal streptokinase production was found at 0.04% KH2PO4, 0.04% MgSO4.7H2O, 0.15% NaHCO3 and 0.04% CaCO3. Finally, the optimized medium resulted in 84.75 U/mg specific activity and 74.5% recovery. The purification process was carried out simultaneously using ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration. Finally, a purified sample of streptokinase was run on SDS-PAGE and resolute 47 kDa molecular weight. The use of β-hemolytic Streptococci to obtain streptokinase is not free from health risks and is related to anaphylaxis. This study provides a way forward for the cost-effective ways to obtain streptokinase for the treatment of thrombosis.

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