Multiplex amplification of Target Genes for Periodontal Pathogens in Continuous Flow PCR Microfluidic Chip

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Zhenqing Li ◽  
Jiahui Liu ◽  
Ping Wang ◽  
Chunxian Tao ◽  
Lulu Zheng ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Target Genes ◽  
Treponema Denticola ◽  
Periodontal Pathogens ◽  
Tannerella Forsythia ◽  
Multiplex Amplification

Porphyromonas gingivalis (P.g), Treponema denticola (T.d), and Tannerella forsythia (T.f) are supposed as the major periodontal pathogens induced gingivitis, which affects 50-90% adults worldwide. Microfluidic chip based on continuous flow...

A Continuous Flow PCR Array Microfluidic Chip Applied for Simultaneous Amplification of Target Genes of Periodontal Pathogens

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Bo Yang ◽  
Ping Wang ◽  
Zhenqing Li ◽  
Chunxian Tao ◽  
Qingxiang You ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Target Genes ◽  
Pcr Array ◽  
Periodontal Pathogens ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Time Required

The concept of time to place conversion makes continuous flow polymerase chain reaction (CF-PCR) microfluidic chip an ideal way to reduce the time required for amplification of target genes, however,...

In vitro Resistance Testing of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to Triclosan

2016 ◽  
Vol 17 (4) ◽  
pp. 282-285 ◽  
Author(s):  
Deema J Farsi ◽  
Anne Tanner
Keyword(s):  
Escherichia Coli ◽  
Porphyromonas Gingivalis ◽  
Vitamin K ◽  
Prolonged Exposure ◽  
Resistance Testing ◽  
Prevotella Intermedia ◽  
Periodontal Pathogens ◽  
Tannerella Forsythia ◽  
Prolonged Incubation

ABSTRACT Aim To determine the sensitivity of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to triclosan, and determine if these bacteria develop resistance to triclosan upon prolonged exposure. Materials and methods Susceptibility to triclosan was tested against three periodontal pathogens P. gingivalis, P. intermedia, and T. forsythia. Escherichia coli strains sensitive and resistant to triclosan were used as biological controls to confirm the efficacy of triclosan in the assays. Agar plates were prepared locally with vitamin K and hemin-supplemented medium. Results Porphyromonas gingivalis and P. intermedia did not grow on plates containing ≥2 μg/ml triclosan, while T. forsythia did not grow on ≥1.66 μg/ml. Colonies of P. intermedia resistant to triclosan developed after prolonged incubation at 2 μg/ml, but this resistance disappeared during subculture in the absence of triclosan. Conclusion No significant resistance to triclosan was detected for these species. Clinical significance Dental products containing triclosan can be beneficial in controlling periodontal disease. How to cite this article Farsi D, Tanner A. In vitro Resistance Testing of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to Triclosan. J Contemp Dent Pract 2016;17(4):282-285.

Presence of archaea and selected bacteria in infected root canal systems

2018 ◽  
Vol 64 (5) ◽  
pp. 317-326 ◽  
Author(s):  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Pawłowska ◽  
Tomasz Płoszaj ◽  
Henryk Witas ◽  
Urszula Godzik ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Porphyromonas Gingivalis ◽  
Root Canal ◽  
Expression Profiles ◽  
Rna Extraction ◽  
Treponema Denticola ◽  
Rrna Gene ◽  
Pulp Tissue ◽  
Tannerella Forsythia

Infections of the root canal have polymicrobial etiology. The main group of microflora in the infected pulp is bacteria. There is limited data that archaea may be present in infected pulp tissue. The aim of this study was to check the prevalence of archaea in necrotic root canal samples obtained from patients with primary or post-treatment infection. The prevalence of selected bacteria species (Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Synergistes sp.) in necrotic samples was evaluated as well. Sixty-four samples from root canal were collected for DNA and RNA extraction. A PCR assay based on the 16S rRNA gene was used to determine the presence of archaea and selected bacteria. Of the 64 samples, 6 were analyzed by semiquantitative reverse transcription PCR to estimate expression profiles of 16S rRNA, and another 9 were selected for direct sequencing. Archaea were detected in 48.4% samples. Statistical analysis indicated a negative association in coexistence between archaea and Treponema denticola (P < 0.05; Pearson’s χ2 test). The main representative of the Archaea domain found in infected pulp tissue was Methanobrevibacter oralis. Archaea 16S rRNA gene expression was significantly lower than Synergistes sp., Porphyromonas gingivalis, and Tannerella forsythia (P < 0.05; Student’s t test). Thus, it can be hypothesized that archaea may participate in the endodontic microbial community.

scholarly journals Mixed Red-Complex Bacterial Infection in Periodontitis

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
N. Suzuki ◽  
M. Yoneda ◽  
T. Hirofuji
Keyword(s):  
Periodontal Disease ◽  
Bacterial Infection ◽  
Porphyromonas Gingivalis ◽  
Treponema Denticola ◽  
Periodontal Tissue ◽  
Tannerella Forsythia

The red complex, which includesPorphyromonas gingivalis,Treponema denticola, andTannerella forsythia(formerlyBacteroides forsythus), are recognized as the most important pathogens in adult periodontal disease. These bacteria are usually found together in periodontal pockets, suggesting that they may cause destruction of the periodontal tissue in a cooperative manner. This article discusses the interspecies pathogenic interactions within the red complex.

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