Virus-templated magnetic composite hydrogels for surface immobilization of mimic-free-lipase

Nanoscale ◽  
2021 ◽  
Author(s):  
Wenjing Qi ◽  
Huimin Yu
Keyword(s):  
Magnetic Composite ◽  
Surface Immobilization ◽  
Enzyme Loading ◽  
Recovery Yield ◽  
Composite Hydrogels ◽  
M13 Virus ◽  
High Enzyme ◽  
Loading Amount ◽  
Enhanced Stability ◽  
Free Lipase

M13 virus-templated magnetic composite hydrogels were designed for surface immobilization of mimic-free-lipases, achieving high enzyme loading amount, high activity recovery yield, free-enzyme-equal activity, less MNPs and enhanced stability.

scholarly journals Switches induced by quorum sensing in a model of enzyme-loaded microparticles

2018 ◽  
Vol 15 (140) ◽  
pp. 20170945 ◽  
Author(s):  
Tamás Bánsági ◽  
Annette F. Taylor
Keyword(s):  
Drug Delivery ◽  
Quorum Sensing ◽  
Cell Density ◽  
Group Size ◽  
Enzyme Concentration ◽  
Autocatalytic Reaction ◽  
Enzyme Loading ◽  
High Enzyme ◽  
Different Types ◽  
Hydrolysis Of

Quorum sensing refers to the ability of bacteria and other single-celled organisms to respond to changes in cell density or number with population-wide changes in behaviour. Here, simulations were performed to investigate quorum sensing in groups of diffusively coupled enzyme microparticles using a well-characterized autocatalytic reaction which raises the pH of the medium: hydrolysis of urea by urease. The enzyme urease is found in both plants and microorganisms, and has been widely exploited in engineering processes. We demonstrate how increases in group size can be used to achieve a sigmoidal switch in pH at high enzyme loading, oscillations in pH at intermediate enzyme loading and a bistable, hysteretic switch at low enzyme loading. Thus, quorum sensing can be exploited to obtain different types of response in the same system, depending on the enzyme concentration. The implications for microorganisms in colonies are discussed, and the results could help in the design of synthetic quorum sensing for biotechnology applications such as drug delivery.

Enzymatic electrochemical biosensor for urea with a polyaniline grafted conducting hydrogel composite modified electrode

RSC Advances ◽  
2016 ◽  
Vol 6 (95) ◽  
pp. 92520-92533 ◽  
Author(s):  
Joyati Das ◽  
Priyabrata Sarkar
Keyword(s):  
Polyvinyl Alcohol ◽  
Conducting Polymer ◽  
Modified Electrode ◽  
Electrochemical Biosensor ◽  
Enzyme Loading ◽  
Polymer Hydrogel ◽  
Hydrogel Composite ◽  
High Enzyme

A new conducting polymer hydrogel (CPH) comprising polyaniline grafted polyvinyl alcohol–polyacrylamide ensured high enzyme loading and urea sensitivity.

Hollow Zeolite Microspheres Give Enzymes a Home: A Hybrid Heterogeneous Catalyst for Chemoenzymatic Cascade Reactions

2019 ◽  
Author(s):  
Valentin Smeets ◽  
Walid Baaziz ◽  
Ovidiu Ersen ◽  
Eric M. Gaigneaux ◽  
Cédric Boissière ◽  
...  
Keyword(s):  
Self Assembly ◽  
Hollow Spheres ◽  
Cascade Reactions ◽  
Mesoporous Zeolite ◽  
Tandem Catalysis ◽  
Hybrid Catalyst ◽  
Enzyme Loading ◽  
Enzymatic Formation ◽  
High Enzyme ◽  
New Strategy

We propose a new strategy for the preparation of a hybrid catalyst based on the TS-1 zeolite and on glucose oxidase. The design allows to combine all the features of the zeolite with a high enzyme loading. The method is based on the aerosol-assisted self-assembly of TS-1 nanocrystals to form hollow mesoporous zeolite microspheres with tailored texture and good epoxidation activity in water. The latter spheres were subsequently loaded with various amounts of enzymes which were then cross-linked to secure their entrapment. Thus, the hollow spheres were used both as catalysts and as vessels for the enzyme. The chemo-enzymatic formation of epoxides by tandem catalysis is demonstrated.

scholarly journals Immobilization of Horseradish Peroxidase on Multi-Armed Magnetic Graphene Oxide Composite

2019 ◽  
Vol 57 (2) ◽  
pp. 260-271 ◽  
Author(s):  
Junhui Rong ◽  
Yun Wang ◽  
Liang Ni ◽  
Wenli Zhang ◽  
Chunmei Li ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Horseradish Peroxidase ◽  
Degradation Rate ◽  
Magnetic Composite ◽  
Support Material ◽  
Amino Groups ◽  
Magnetic Graphene Oxide ◽  
Magnetic Graphene ◽  
Loading Amount ◽  
First Time

In this study, a novel type of multi-armed polymer (poyltehylene glycol, PEG) magnetic graphene oxide (GO) composite (GO@Fe3O4@6arm-PEG-NH2) has been synthesized as a support for immobilization of horseradish peroxidase (HRP) for the first time. The loading amount of HRP was relatively high (186.34 mg/g) due to the surface of carrier material containing a large amount of amino groups from 6arm-PEG-NH2, but degradation rate of phenols was also much higher (95.4 %), which is attributed to the synergistic effect between the free HRP (45.4 %) and the support material of GO@Fe3O4@6arm-PEG-NH2 (13.6 %). Compared with the free enzyme, thermal, storage and operational stability of the immobilized HRP improved. The immobilized HRP still retained over 68.1 % activity after being reused 8 times. These results suggest that the multi-armed magnetic composite has good application prospect for enzyme immobilization.

Effect of β-Glucosidase and Gleditsia Saponin on Enzymatic Hydrolysis Process of Furfural Residues

2011 ◽  
Vol 236-238 ◽  
pp. 456-459 ◽  
Author(s):  
Dan Qing Zhao ◽  
Yue Feng ◽  
Li Wei Zhu ◽  
Jian Xin Jiang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Optimal Conditions ◽  
Enzyme Loading ◽  
Glucosidase Activity ◽  
Furfural Residues ◽  
Process Configuration ◽  
Hydrolysis Process ◽  
High Enzyme ◽  
Process Cost

Enzymatic hydrolysis constitutes an attractive process configuration for ethanol production from biomass. However, the high enzyme addition contributes to a high process cost. This study shows that appropriate concentration of Gleditsia saponin could reduce the enzyme loading and increase β-glucosidase active. The optimal conditions of enzymatic hydrolysis process of furfural residues was at enzyme loading of 6 FPU cellulose/g substrate and 9 IU β-glucosidase/g substrate with 0.6 g/l of Gleditsia saponin. The enzyme loading could be reduced by 50% with addition of 0.64 g/l Gleditsia saponin during furfural residues hydrolysis. The β-glucosidase activity from hydrolysate with 0.64 g/l Gleditsia saponin was higher than that without any surfactants.

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