Effect of β-Glucosidase and Gleditsia Saponin on Enzymatic Hydrolysis Process of Furfural Residues

2011 ◽  
Vol 236-238 ◽  
pp. 456-459 ◽  
Author(s):  
Dan Qing Zhao ◽  
Yue Feng ◽  
Li Wei Zhu ◽  
Jian Xin Jiang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Optimal Conditions ◽  
Enzyme Loading ◽  
Glucosidase Activity ◽  
Furfural Residues ◽  
Process Configuration ◽  
Hydrolysis Process ◽  
High Enzyme ◽  
Process Cost

Enzymatic hydrolysis constitutes an attractive process configuration for ethanol production from biomass. However, the high enzyme addition contributes to a high process cost. This study shows that appropriate concentration of Gleditsia saponin could reduce the enzyme loading and increase β-glucosidase active. The optimal conditions of enzymatic hydrolysis process of furfural residues was at enzyme loading of 6 FPU cellulose/g substrate and 9 IU β-glucosidase/g substrate with 0.6 g/l of Gleditsia saponin. The enzyme loading could be reduced by 50% with addition of 0.64 g/l Gleditsia saponin during furfural residues hydrolysis. The β-glucosidase activity from hydrolysate with 0.64 g/l Gleditsia saponin was higher than that without any surfactants.

scholarly journals PRE-TREATMENT AND ENZYMATIC HYDROLYSIS OF BANANA (Musa acuminata x balbisiana) PSEUDOSTEM FOR ETHANOL PRODUCTION

2020 ◽  
Vol 3 (2) ◽  
pp. 98-107
Author(s):  
Galileo E. Araguirang ◽  
Arianne Joyce R. Arizala ◽  
Eden Beth B. Asilo ◽  
Jamie Louise S. Batalon ◽  
Erin B. Bello ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Incubation Time ◽  
Enzyme Concentration ◽  
The Philippines ◽  
Solid Loading ◽  
Hydrolysis Process ◽  
Pre Treatment ◽  
Hydrolysis Of ◽  
Second Generation Ethanol

Banana (M. acuminata x balbisiana) is an abundant lignocellulosic waste material in large plantations all over the Philippines, especially in Mindanao, which can be utilized as substrate in producing high-value products like ethanol. To compensate for the low yield based on total weight of substrate due to the high moisture content of banana pseudostem, there is the primary challenge to make the conversion of this lignocellulosic biomass into monomeric sugar and then into ethanol more efficiently in order to achieve yields that would make it cost-competitive. Hence, this study evaluated the effects of solid loading, incubation time and amount of enzyme on yield of reducing sugars in the enzymatic hydrolysis process and attempted to optimize the significant factors by Response Surface Methodology (RSM), specifically using Box-Behnken design. There was significant improvement on the reducing sugar yield of the pretreated banana pseudostem at 20 h incubation time, 15 g solid loading and 0.55 % enzyme concentration. Ethanol production was observed to be higher in the detoxified substrate although biomass was higher for the non-detoxified substrate. As to our knowledge, the present study is the first attempt to produce second generation ethanol using banana pseudostem waste as feedstock in the Philippines.

scholarly journals OLIGO-GLUCOMANNAN PRODUCTION FROM PORANG (Amorphophallus oncophyllus) GLUCOMANNAN BY ENZYMATIC HYDROLYSIS USING β-MANNANASE

2021 ◽  
Vol 17 (1) ◽  
pp. 23
Author(s):  
Anggela Anggela ◽  
Widiastuti Setyaningsih ◽  
Santad Wichienchot ◽  
Eni Harmayani
Keyword(s):  
Reaction Time ◽  
Enzymatic Hydrolysis ◽  
Optimum Condition ◽  
Hplc Analysis ◽  
Degree Of Polymerization ◽  
Reducing Sugar ◽  
Optimal Conditions ◽  
Hydrolysis Process ◽  
Alternative Approach ◽  
Potential Substrate

Porang (Amorphophallus oncophyllus) is an indigenous tuber of Indonesia that rich in glucomannan. An alternative approach to produce porang oligo-glucomannan (POG) as prebiotic from porang glucomannan (PGM) was made by enzymatic hydrolysis using β-mannanase. This study aimed to  produce POG under optimal conditions by controlled enzymatic hydrolysis process. The PGM flour contained 96.12% of indigestible carbohydrates. The optimum condition of enzymatic hydrolysis producing the highest reducing sugar was as follows: temperature 37°C, pH 5.5, a ratio of enzyme to the substrate (E/S) 1:1000, and reaction time 4 h. HPLC analysis confirmed that 99.45% of the resulting POG consisted of oligosaccharides with a degree of polymerization (DP) 3. Hence, the PGM utilized in this study has been proven as a potential substrate for POG production. Additionally, the resulting POG was considered as a functional ingredient due to has prebiotic potential.

scholarly journals Effect of lignin content on enzymatic hydrolysis of furfural residues

BioResources ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 317-328 ◽  
Author(s):  
Ran Sun ◽  
Xianliang Song ◽  
Runcang Sun ◽  
Jianxin Jiang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignin Content ◽  
Enzymatic Saccharification ◽  
Cost Effective ◽  
Raw Material ◽  
Enzyme Loading ◽  
Glucose Yield ◽  
Lignin Removal ◽  
Furfural Residues ◽  
Enzyme Dosage

The enzymatic saccharification of pretreated furfural residues with different lignin content was studied to verify the effect of lignin removal in the hydrolysis process. The results showed that the glucose yield was improved by increasing the lignin removal. A maximum glucose yield of 96.8% was obtained when the residue with a lignin removal of 51.4% was hydrolyzed for 108 h at an enzyme loading of 25 FPU/g cellulose. However, further lignin removal did not increase the hydrolysis. The effect of enzyme loading on the enzymatic hydrolysis was also explored in this work. It was concluded that a high glucose yield of 90% was achieved when the enzyme dosage was reduced from 25 to 15 FPU/g cellulose, which was cost-effective for the sugar and ethanol production. The structures of raw material and delignified samples were further characterized by XRD and scanning electron microscopy (SEM).

Use of ionic liquids for improvement of cellulosic ethanol production

BioResources ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 1-2
Author(s):  
Qijun Wang ◽  
Yuanxin Wu ◽  
Shengdong Zhu
Keyword(s):  
Ionic Liquids ◽  
Enzymatic Hydrolysis ◽  
Ethanol Production ◽  
Lignocellulosic Biomass ◽  
Cellulosic Ethanol ◽  
Heterogeneous Reaction ◽  
Reaction System ◽  
The Other ◽  
Fermentable Sugars ◽  
Hydrolysis Process

Cellulosic ethanol production has drawn much attention in recent years. However, there remain significant technical challenges before such production can be considered as economically feasible at an industrial scale. Among them, the efficient conversion of carbohydrates in lignocellulosic biomass into fermentable sugars is one of the most challenging technical difficulties in cellulosic ethanol production. Use of ionic liquids has opened new avenues to solve this problem by two different pathways. One is pretreatment of lignocellulosic biomass using ionic liquids to increase its enzymatic hydrolysis efficiency. The other is to transform the hydrolysis process of lignocellulosic biomass from a heterogeneous reaction system to a homogeneous one by dissolving it into ionic liquids, thus improving its hydrolysis efficiency.

scholarly journals Conversion of Lignocellulosic Material Into Fermentable Sugars

2017 ◽  
Vol 12 (4) ◽  
pp. 141-153
Author(s):  
Asem Hassan Mohammed ◽  
Frank Behrendt Behrendt ◽  
Frank Jürgen Methner
Keyword(s):  
Enzymatic Hydrolysis ◽  
Structural Features ◽  
Impact Factors ◽  
Optimal Conditions ◽  
Short Term ◽  
Water Loading ◽  
Lime Pretreatment ◽  
Hydrolysis Process ◽  
Biomass Materials ◽  
Optimal Values

Enzymatic hydrolysis process of lignocellulosic biomass materials is difficult because of inherent structural features of biomass, which represents barriers that prevent complete hydrolysis; therefore, pretreatment techniques are necessary to render biomass highly digestible in enzymatic hydrolysis process. In this research, (non?) oxidative short-term lime pretreatment of willow wood was used. A weight of  11.40 g of willow wood was mixed with an excess of calcium hydroxide (0.4 g Ca(OH)2/g raw biomass) and water loading (15 g/g raw biomass). Lime pretreatment was carried out for various periods of time including 1, 2, 3.5, 5 and 6 h, with temperatures at 100, 113, 130, 147 and 1600C, and oxygen pressures as oxidativeagent (6, 9, 13.5, 17.8, 21 bar absolute). The optimization of both pretreatment and enzymatic hydrolysis were depended on the maximum overall yields of glucan and xylan after two processes of lime pretreatment and enzymatic hydrolysis. The optimal conditions of pretreatment were as follow: 1) 1.33 h, 1470C, 17.8 bar absolute, 0.26 g Ca(OH)2/g raw biomass. 2) 1.25 h, 155 0C, 21 bar absolute, 0.26 Ca(OH)2/g raw biomass. Furthermore, the optimal values for low impact factors such as water loading was 15 g/g raw biomass and particle size was less than 3 mm. The optimal conditions of enzymatic hydrolysis were as follow: Cellulase enzymeloading was 0.1 g /g glucan in raw biomass, at substrate concentration of 50 g/L during 72 h of enzymatic hydrolysis The yield of enzymatic hydrolysis under these conditions were as follow: 96.00 g glucan/100 g of glucan in raw biomass, and 65.00 g xylan/100 g xylan in raw biomass.

Improvement of Enzymatic Hydrolysis of Whole Slurry from Bisulfite-Pretreated Furfural Residues with Low Enzyme Loading

2015 ◽  
Vol 9 (5) ◽  
pp. 509-514 ◽  
Author(s):  
Tianran Zheng ◽  
Yang Xing ◽  
Yongmiao Wang ◽  
Lingxi Bu ◽  
Jianxin Jiang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzyme Loading ◽  
Furfural Residues ◽  
Whole Slurry ◽  
Hydrolysis Of

Enzymatic Hydrolysis of Lignocellulosic Oil Palm Empty Fruit Bunch (EFB)

2015 ◽  
Vol 1113 ◽  
pp. 305-310
Author(s):  
Qadly Ameen Pahlawi ◽  
Nazlee Faisal Ghazali ◽  
Khairilanuar Mohd Hanim ◽  
Nik Azmi Nik Mahmood
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Reaction ◽  
Cellulose Hydrolysis ◽  
Enzyme Concentration ◽  
Empty Fruit Bunch ◽  
Enzyme Adsorption ◽  
Enzyme Loading ◽  
Cellulose Conversion ◽  
Hydrolysis Process ◽  
Hydrolysis Of

A preliminary study was performed on enzymatic hydrolysis process for treating empty fruit bunch (EFB) fibre. The bioconversion of cellulose hydrolysis was carried out with soluble cellulase from Trichodermareesei as the biocatalyst. Crucial trends such as substrate and enzyme loading influencing the enzymatic reaction were also studied in order to enhance the cellulose conversion. The results indicate that as the enzyme loading was increased, the EFB conversion also increased until it reached 115.63 FPU/g of enzyme concentration, beyond this values, the reverse occurred. On the other hand, as the substrate loading was increased the conversion decreased. Inhibition of enzyme adsorption by hydrolysis products appear to be the main cause of the decreasing conversion at increasing enzyme loading and substrate loading.

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