scholarly journals Large-scale synthesis of a monophosphonated tetrathiatriarylmethyl spin probe for concurrent in vivo measurement of pO2, pH and inorganic phosphate by EPR

RSC Advances ◽  
2021 ◽  
Vol 11 (42) ◽  
pp. 25951-25954
Author(s):  
Teresa D. Gluth ◽  
Martin Poncelet ◽  
Stephen DeVience ◽  
Marieta Gencheva ◽  
Emily. H. Hoblitzell ◽  
...  
Keyword(s):  
Inorganic Phosphate ◽  
Spin Probe ◽  
Large Scale ◽  
In Vivo Measurement ◽  
New Strategy ◽  
Large Scale Synthesis ◽  
User Friendly ◽  
Spectrum Fitting

We report a new strategy for the synthesis of a mono-phosphonated triarylmethyl radical spin probe and a standalone application with a user-friendly interface for automatic spectrum fitting and extraction of pO2, pH, and [Pi] values.

ChemInform Abstract: Strategies for Large-Scale Synthesis of Coelenterazine for in vivo Applications.

ChemInform ◽  
2014 ◽  
Vol 45 (33) ◽  
pp. no-no
Author(s):  
Tej B. Shrestha ◽  
Deryl L. Troyer ◽  
Stefan H. Bossmann
Keyword(s):  
Large Scale ◽  
Large Scale Synthesis

In Vivo Measurement of Human Nasal Mucociliary Motility Using a Laser Light Scattering Instrument

1997 ◽  
Vol 106 (10) ◽  
pp. 859-862 ◽  
Author(s):  
Yoav Paltieli ◽  
Ludwig Podoshin ◽  
Milo Fradis ◽  
Hala Shiti ◽  
Jacob Ben-David ◽  
...  
Keyword(s):  
Light Scattering ◽  
Defense Mechanisms ◽  
Laser Light ◽  
Normal Subjects ◽  
Laser Light Scattering ◽  
In Vivo Measurement ◽  
Fiberoptic Probe ◽  
The Mean ◽  
User Friendly

The mucociliary system is one of the most important airway defense mechanisms, and knowledge of the mucociliary wave frequency (MWF) is important in the understanding of this system. Employing a laser light scattering technique and a thin, flexible fiberoptic probe, we developed and tested a simple and practical device for real-time in vivo measurements of mucociliary activity in the human nose. The laser instrument is user-friendly and does not produce any discomfort to the patient. The mean ± SE of MWF of 36 measurements in 16 normal subjects was 7.7 ± 0.5 Hz. The mean MWF of 17 measurements in 7 patients with allergic rhinitis was 5.5 ± 0.2 Hz (p < .005), and the mean MWF of 56 measurements in 17 patients with septum deviation was 5.8 ± 0.2 Hz (p < .001). The instrument presented in this study might provide a new and convenient method of studying the mucociliary activity in the respiratory tract.

Biocompatible, Luminescent Silver@Phenol Formaldehyde Resin Core/Shell Nanospheres: Large-Scale Synthesis and Application for In Vivo Bioimaging

2008 ◽  
Vol 18 (6) ◽  
pp. 872-879 ◽  
Author(s):  
Shi-Rui Guo ◽  
Jun-Yan Gong ◽  
Peng Jiang ◽  
Mian Wu ◽  
Yang Lu ◽  
...  
Keyword(s):  
Large Scale ◽  
Phenol Formaldehyde ◽  
Phenol Formaldehyde Resin ◽  
Formaldehyde Resin ◽  
Core Shell ◽  
Resin Core ◽  
Large Scale Synthesis

scholarly journals A new strategy for large-scale synthesis of Na0.5Bi0.5TiO3 nanowires and their application in piezocatalytic degradation

2021 ◽  
Author(s):  
Rui Huang ◽  
Jiang Wu ◽  
Enzhu Lin ◽  
Zihan Kang ◽  
Ni Qin ◽  
...  
Keyword(s):  
Hydrothermal Method ◽  
Organic Pollutants ◽  
Large Scale ◽  
New Strategy ◽  
Large Scale Synthesis

Na0.5Bi0.5TiO3 nanowires prepared by the template hydrothermal method exhibited excellent piezocatalytic activity in the degradation of organic pollutants.

Large-Scale Synthesis of Bi2S3Nanodots as a Contrast Agent for In Vivo X-ray Computed Tomography Imaging

2011 ◽  
Vol 23 (42) ◽  
pp. 4886-4891 ◽  
Author(s):  
Kelong Ai ◽  
Yanlan Liu ◽  
Jianhua Liu ◽  
Qinghai Yuan ◽  
Yangyang He ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Contrast Agent ◽  
Large Scale ◽  
Computed Tomography Imaging ◽  
X Ray ◽  
Tomography Imaging ◽  
X Ray Computed ◽  
Large Scale Synthesis

Strategies for Large-Scale Synthesis of Coelenterazine for in Vivo Applications

Synthesis ◽  
2014 ◽  
Vol 46 (05) ◽  
pp. 646-652 ◽  
Author(s):  
Tej Shrestha ◽  
Stefan Bossmann ◽  
Deryl Troyer
Keyword(s):  
Large Scale ◽  
Large Scale Synthesis

scholarly journals CRISP-view: a database of functional genetic screens spanning multiple phenotypes

2020 ◽  
Vol 49 (D1) ◽  
pp. D848-D854
Author(s):  
Yingbo Cui ◽  
Xiaolong Cheng ◽  
Qing Chen ◽  
Bicna Song ◽  
Anthony Chiu ◽  
...  
Keyword(s):  
Genetic Screening ◽  
Large Scale ◽  
Genetic Screens ◽  
Analysis Pipeline ◽  
Systematic Analysis ◽  
Multiple Phenotypes ◽  
Rapid Accumulation ◽  
User Friendly

Abstract High-throughput genetic screening based on CRISPR/Cas9 or RNA-interference (RNAi) enables the exploration of genes associated with the phenotype of interest on a large scale. The rapid accumulation of public available genetic screening data provides a wealth of knowledge about genotype-to-phenotype relationships and a valuable resource for the systematic analysis of gene functions. Here we present CRISP-view, a comprehensive database of CRISPR/Cas9 and RNAi screening datasets that span multiple phenotypes, including in vitro and in vivo cell proliferation and viability, response to cancer immunotherapy, virus response, protein expression, etc. By 22 September 2020, CRISP-view has collected 10 321 human samples and 825 mouse samples from 167 papers. All the datasets have been curated, annotated, and processed by a standard MAGeCK-VISPR analysis pipeline with quality control (QC) metrics. We also developed a user-friendly webserver to visualize, explore, and search these datasets. The webserver is freely available at http://crispview.weililab.org.

Large-Scale Synthesis of a Meso-Substituted Dipyrromethane

10.1039/sp248 ◽  
2006 ◽  
Author(s):  
Joydev K. Laha
Keyword(s):  
Large Scale ◽  
Large Scale Synthesis

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

The Effect of Active Site-inhibited Factor VIIa on Tissue Factor-initiated Coagulation Using Platelets before and after Aspirin Administration

1997 ◽  
Vol 78 (04) ◽  
pp. 1202-1208 ◽  
Author(s):  
Marianne Kjalke ◽  
Julie A Oliver ◽  
Dougald M Monroe ◽  
Maureane Hoffman ◽  
Mirella Ezban ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Active Site ◽  
Large Scale ◽  
Thrombin Generation ◽  
Factor Viia ◽  
Dependent Manner ◽  
Antithrombotic Agent ◽  
Before And After ◽  
Ic50 Values

SummaryActive site-inactivated factor VIIa has potential as an antithrombotic agent. The effects of D-Phe-L-Phe-L-Arg-chloromethyl ketone-treated factor VIla (FFR-FVIIa) were evaluated in a cell-based system mimicking in vivo initiation of coagulation. FFR-FVIIa inhibited platelet activation (as measured by expression of P-selectin) and subsequent large-scale thrombin generation in a dose-dependent manner with IC50 values of 1.4 ± 0.8 nM (n = 8) and 0.9 ± 0.7 nM (n = 7), respectively. Kd for factor VIIa binding to monocytes ki for FFR-FVIIa competing with factor VIIa were similar (11.4 ± 0.8 pM and 10.6 ± 1.1 pM, respectively), showing that FFR-FVIIa binds to tissue factor in the tenase complex with the same affinity as factor VIIa. Using platelets from volunteers before and after ingestion of aspirin (1.3 g), there were no significant differences in the IC50 values of FFR-FVIIa [after aspirin ingestion, the IC50 values were 1.7 ± 0.9 nM (n = 8) for P-selectin expression, p = 0.37, and 1.4 ± 1.3 nM (n = 7) for thrombin generation, p = 0.38]. This shows that aspirin treatment of platelets does not influence the inhibition of tissue factor-initiated coagulation by FFR-FVIIa, probably because thrombin activation of platelets is not entirely dependent upon expression of thromboxane A2.

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