Fiberoptic Probe For Hemodynamic Spectroscopy in Freely Moving Mice Shows Cerebrovascular Dysregulation In Alzheimers and Glioma Models

Significance: Cerebral vascular reactivity is critical parameters of brain homeostasis in health and disease, but the investigational value of brain oxymetry is diminished by anesthesia and mechanical fixation of the mouse scull. Aim: We needed to reduce the physical restrictivity of hemodynamic spectroscopy to enable cancer and Alzheimers disease (AD) studies in freely-moving mice. Approach: We combined spectroscopy, spectral analysis software and a magnetic, implantable device to measure vascular reactivity in unanesthetized, freely-moving mice. We measured cerebral blood volume fraction (CBVF) and oxygen saturation (SO2). Results: CBVF and SO2 demonstrated delayed cerebrovascular recovery from hypoxia in an orthotopic xenograft glioma model and we found increased CBVF during hypercapnia in a mouse model of AD compared to wild-type littermates. Conclusions: Our optomechanical approach to reproducibly getting light into and out of the brain enabled us to successfully measure CBVF and SO2 during hypercapnia in unanesthetized freely-moving mice. We present hardware and software enabling oximetric analysis of metabolic activity, which provides a safe and reliable method for rapid assessment of vascular reactivity in murine disease models as well as CBVF and SO2.

Low-cost Fiberoptic Probe for Ammonia Early Detection in Fish Farms

Recirculating aquaculture systems (RAS) are complex systems in which there is an interaction between the fish biomass and water chemistry, where small variations in the environment can lead to major effects in the production. Ammonia is one of the key limiting factors in RAS and its early detection in small concentrations prevents fish mortality and improves the production quality. Aiming at this background, this paper presents a low-cost fiberoptic probe for the early detection of ammonia. The sensor was based on the chemical interaction between the Oxazine 170 perchlorate layer, deposited in an uncladed polymer optical fiber (POF), and the ammonia dissolved in water. In addition, a thin metallic layer (composed by gold and palladium) was deposited in the fiber end facet and acted as a reflector for the optical signals, enabling the use of the proposed sensor in reflection mode. Different configurations of the sensor were tested, where the effects of polydimethylsiloxane (PDMS) protective layer, thermal treatments, and the use on reflection or transmission modes were compared in the assessment of ammonia concentrations in the range of 100 ppb to 900 ppb. Results showed a better performance (as a function of the sensor sensitivity and linearity) of the sensor with the annealing thermal treatment and without the PDMS layer. Then, the proposed fiberoptic probe was applied on the ammonia detection in high-salinity water, where ammonia concentrations as low as 100 ppb were detected.

Early optical detection of cerebral edema in vivo

Object Cerebral edema is a significant cause of morbidity and mortality in diverse disease states. Currently, the means to detect progressive cerebral edema in vivo includes the use of intracranial pressure (ICP) monitors and/or serial radiological studies. However, ICP measurements exhibit a high degree of variability, and ICP monitors detect edema only after it becomes sufficient to significantly raise ICP. The authors report the development of 2 distinct minimally invasive fiberoptic near-infrared (NIR) techniques able to directly detect early cerebral edema. Methods Cytotoxic brain edema was induced in adult CD1 mice via water intoxication by intraperitoneal water administration (30% body weight intraperitoneally). An implantable dual-fiberoptic probe was stereotactically placed into the cerebral cortex and connected to optical source and detector hardware. Optical sources consisted of either broadband halogen illumination or a single-wavelength NIR laser diode, and the detector was a sensitive NIR spectrometer or optical power meter. In one subset of animals, a left-sided craniectomy was performed to obtain cortical biopsies for water-content determination to verify cerebral edema. In another subset of animals, an ICP transducer was placed on the contralateral cortex, which was synchronized to a computer and time stamped. Results Using either broadband illumination with NIR spectroscopy or single-wavelength laser diode illumination with optical power meter detection, the authors detected a reduction in NIR optical reflectance during early cerebral edema. The time intervals between water injection (Time Point 0), optical trigger (defined as a 2-SD change in optical reflectance from baseline), and defined threshold ICP values of 10, 15 and 20 mm Hg were calculated. Reduction in NIR reflectance occurred significantly earlier than any of the ICP thresholds (p < 0.001). Saline-injected control mice exhibited a steady baseline optical signal. There was a significant correlation between reflectance change and tissue specific gravity of the cortical biopsies, further validating the dual-fiberoptic probe as a direct measure of cerebral edema. Conclusions Compared with traditional ICP monitoring, the aforementioned minimally invasive NIR techniques allow for the significantly earlier detection of cerebral edema, which may be of clinical utility in the identification and thus early treatment of cerebral edema.

Monitoring the Presence of Humic Substances in Wool and Silk by the Use of Nondestructive Fluorescence Spectroscopy: Quality Control for 14C Dating of Wool and Silk

Radiocarbon dating of degraded wool and silk provides 14C results of questionable reliability. In most cases, degraded wool/silk contains humic substances (HSs). Thus, a nondestructive fluorescence spectroscopy method, using a fiberoptic probe, was developed to monitor the presence of HSs in degraded wool and silk. This method can provide information about the presence of HSs before and after pretreatment and about the 14C age reliability. This study suggests considering with care wool/silk samples 14C dating wherein HSs are detected, because the conventional solvent pretreatment method using a NaOH wash is in most cases not sufficient to remove all humic substance contaminants. As a result, unreliable 14C dates can be provided.