scholarly journals A comparison of the relationships between progestin receptors and oestrogen receptors in neural and non-neural target tissues of the rat during the oestrous cycle

1980 ◽  
Vol 190 (3) ◽  
pp. 691-695 ◽  
Author(s):  
S Thrower ◽  
L Lim
Keyword(s):  
Cerebral Cortex ◽  
Oestrogen Receptor ◽  
Oestrous Cycle ◽  
Oestrogen Receptors ◽  
Progestin Receptors ◽  
Progestin Receptor ◽  
Cyclic Changes ◽  
Relationship Of ◽  
The Relationship

Similar cyclic changes in the content of nuclear oestrogen receptor occurred in the hypothalamus, cerebral cortex, uterus and pituitary during the oestrous cycle. The relationship of the unoccupied to the total nuclear oestrogen receptor at each phase was similar in all these tissues. However, cyclic changes in the content of the cytosol progestin receptor occurred only in the uterus and pituitary (where they paralleled changes in the nuclear oestrogen receptor), but not in the hypothalamus or cerebral cortex.

scholarly journals The unoccupied nuclear oestradiol receptor in the rat uterus and hypothalamus during the oestrous cycle

1981 ◽  
Vol 194 (3) ◽  
pp. 667-671 ◽  
Author(s):  
S Thrower ◽  
C Neethling ◽  
J O White ◽  
L Lim
Keyword(s):  
Nuclear Receptor ◽  
Oestrogen Receptor ◽  
Oestrous Cycle ◽  
Oestrogen Receptors ◽  
Rat Uterus ◽  
Immature Rat ◽  
High Affinity ◽  
Receptor Component ◽  
Cyclic Changes

The nuclear oestrogen receptor population in the rat uterus contained an unoccupied receptor component that bound oestradiol with the high affinity (Kd congruent to 0.5 nM) characteristic of oestrogen receptors. This unoccupied receptor was present at all phases of the oestrous cycle. Its content changed in parallel with that of the total nuclear receptor during the cycle. Oestradiol administration to the immature rat resulted in increases in the uterine content of long-term nuclear receptors (i.e., those still present 8 h after administration); these increases were due to occupied oestrogen receptors, since the content of unoccupied receptor was unchanged. Our previous experiments [White & Lim (1980) Biochem. J. 190, 833-837] have shown in contrast, that oestradiol administration results in an increase in the content of unoccupied nuclear receptor in the hypothalamus. However, as in the uterus, similar cyclic changes in the content of unoccupied nuclear receptor occurred in parallel with those of the total nuclear receptor population in the hypothalamus. Differences and similarities between the unoccupied nuclear receptor of the uterus and hypothalamus are briefly discussed.

scholarly journals The relationship of the oestrogen and progestin receptors in the abnormal uterus of the adult anovulatory rat. Effects of neonatal treatment with testosterone propionate or clomiphene citrate

1981 ◽  
Vol 196 (2) ◽  
pp. 557-565 ◽  
Author(s):  
J O White ◽  
P A Moore ◽  
M G Elder ◽  
L Lim
Keyword(s):  
Oestrogen Receptor ◽  
Testosterone Propionate ◽  
Clomiphene Citrate ◽  
Treated Group ◽  
Progestin Receptors ◽  
Treatment Groups ◽  
Receptor Interactions ◽  
Neonatal Administration ◽  
Relationship Of ◽  
The Relationship

The neonatal administration of testosterone propionate to Wistar rats resulted in anovulatory adults in persistent vaginal oestrus. Clomiphene citrate had a similar effect. In both groups of adults, hyperplasia of the uterine epithelium and occasional metaplasia was observed. The uterine nuclear and cytosol oestrogen and progestin receptors of these anovulatory rats were found to have affinities for their respective ligands similar to those of normal females. The nuclear oestrogen receptor comprised occupied and unoccupied components, as in normal females. The content of the nuclear oestrogen receptor was comparable with that of females in the late dioestrous or pro-oestrous phase. This content was higher in the clomiphene-treated group. Despite the relatively high nuclear oestrogen receptor content the content of progestin receptors, a putative index of the oestrogenic response, was lower in the treated rats than in normal adult females throughout the cycle. Administration of oestradiol to both treatment groups resulted in depletion of cytosol oestrogen receptor content 1 h later, which, however, was not reflected by an increase in the content of nuclear oestrogen receptors. There was no measurable increase in progesterone receptor content in treated rats after daily administration of oestrogen (5 microgram/rat) for 3 days. These changes in sex-hormone-receptor interactions involving an impairment of the normal oestrogenic response may be associated with the abnormal differentiation of the uterus in these sterile, anovulatory animals.

Growth of mouse endometrial luminal epithelial cells in vitro: functional integrity of the oestrogen receptor system and failure of oestrogen to induce proliferation

1991 ◽  
Vol 128 (1) ◽  
pp. 115-120 ◽  
Author(s):  
F.-D. A. Uchima ◽  
M. Edery ◽  
T. Iguchi ◽  
H. A. Bern
Keyword(s):  
Epithelial Cells ◽  
Oestrogen Receptor ◽  
Continuous Exposure ◽  
Oestrogen Receptors ◽  
Serum Free Medium ◽  
Free Medium ◽  
Receptor System ◽  
Serum Free ◽  
Progestin Receptors ◽  
Luminal Epithelial Cells

ABSTRACT Normal endometrial luminal epithelial cells isolated from ovariectomized approximately 40-day-old BALB/cCrgl mice were purified by Percoll density gradient centrifugation and grown as primary cultures in collagen gel matrix and serum-free medium. Cells increased threefold in number during the 9-day culture period. Deletion of insulin, epidermal growth factor or bovine serum albumin resulted in decreased growth. Addition of any single factor to the unsupplemented medium had no effect. Relatively high levels of cytosolic oestrogen receptors and progestin receptors were demonstrable in the cultures. Addition of oestrogen did not enhance epithelial cell proliferation. On the contrary, all doses of oestrogen (180 fmol/l to 218 nmol/l) were inhibitory. Continuous exposure to oestradiol-17β (1·8 nmol/l) for 9 days in serum-free medium resulted in a decrease in cytosolic oestrogen receptors with an associated nuclear accumulation of oestrogen receptors. A corresponding increase in cytosolic progestin receptors was also observed, indicating that no qualitative modification of the oestrogen receptor system had occurred. Thus, as previously reported for vaginal epithelial cells, oestrogen, despite its stimulation of specific product synthesis (progestin receptors), did not increase proliferation of endometrial luminal epithelial cells in this culture system. Journal of Endocrinology (1991) 128, 115–120

scholarly journals Modulation of the renin–angiotensin–aldosterone system by steroid hormones during the oestrous cycle in mares

2020 ◽  
Vol 68 (1) ◽  
pp. 79-84
Author(s):  
Katy Satué ◽  
Paloma Montesinos ◽  
Ana MuÑoz
Keyword(s):  
Steroid Hormones ◽  
Oestrous Cycle ◽  
Laboratory Animals ◽  
Mineralocorticoid Receptors ◽  
Renin Angiotensin Aldosterone System ◽  
Renin Angiotensin ◽  
Positive Correlations ◽  
Relationship Of ◽  
The Relationship ◽  
Circulating Levels

AbstractIn women and females of different species of laboratory animals, oestrogens stimulate the renin–angiotensin–aldosterone system (RAAS) by increasing tissue and circulating levels of angiotensinogen and renin during the preovulatory period. Progesterone and cortisol compete with aldosterone for mineralocorticoid receptors, which results in increased Na+ reabsorption during the postovulatory period. The purpose of the current research was to analyse the relationship of oestradiol-17β, progesterone and cortisol with RAAS in 23 mares during an oestrous cycle. During the preovulatory period, significant positive correlations of oestradiol-17β with renin and aldosterone concentrations and negative correlations of progesterone with renin and aldosterone concentrations were found. In contrast, during the postovulatory period, oestradiol-17β concentrations were positively correlated with angiotensin concentrations and progesterone was negatively correlated with this component of the RAAS. Cortisol concentrations were not correlated with the hormones of the RAAS, neither before nor after ovulation. This research demonstrates that, as occurs in other species, changes in the RAAS during the periovulatory period in mares may be modulated by variations in the concentrations of steroid hormones.

EFFECT OF AN INTRA-UTERINE DEVICE ON INTRACELLULAR RELATIONSHIPS OF THE UTERINE OESTROGEN RECEPTOR, PARTICULARLY DURING PREGNANCY

1980 ◽  
Vol 87 (3) ◽  
pp. 357-364 ◽  
Author(s):  
LESLIE MYATT ◽  
GAUTAM CHAUDHURI ◽  
M. G. ELDER ◽  
LOUIS LIM
Keyword(s):  
Protein Synthesis ◽  
Nuclear Receptor ◽  
Oestrogen Receptor ◽  
Oestrous Cycle ◽  
Blastocyst Implantation ◽  
High Concentrations ◽  
Cyclic Changes ◽  
And Control ◽  
Nuclear Content

The presence of an intra-uterine device in the rat results in a lower nuclear concentration of the oestrogen receptor in the treated horn at pro-oestrus when it is compared with the contralateral control horn. This effect was also seen after the administration of hyperphysiological doses of oestradiol and when the horn was exposed in vitro to high concentrations of oestradiol. The cyclic changes during the oestrous cycle in the activity of the oestrogen-induced enzyme peroxidase were similar in the treated and control horns. These observations have discounted the possibility that the relatively lower nuclear receptor content in the treated horn at pro-oestrus was due to a decreased exposure to oestrogen. A significantly lower nuclear content was also observed in the treated horn on days 4 and 5 of pregnancy. This was not associated with a deficiency in cytosol receptor content which increased concurrently with that of the control horn in the 6 days of pregnancy that were studied. The proportional content of the putative cytosol factor implicated in receptor translocation was similar in both horns, increasing on days 4 and 6 in concert with reported changes in 'induced protein' synthesis. There appeared to be reduced levels of nuclear receptor at a time when blastocyst implantation normally occurs.

Evoked slow and unit responses of the cerebral cortex following repetitive stimulation

1960 ◽  
Vol 198 (3) ◽  
pp. 511-514
Author(s):  
L. L. Boyarsky ◽  
G. Sant'Ambrogio ◽  
D. Frazier
Keyword(s):  
Cerebral Cortex ◽  
Sensorimotor Cortex ◽  
Repetitive Stimulation ◽  
Cortical Mapping ◽  
Complete Disappearance ◽  
Slow Potentials ◽  
Relationship Of ◽  
The Relationship ◽  
Frequency Of Stimulation ◽  
Stimulation Of

Unit and slow primary responses have been compared in the sensorimotor cortex of the cat to stimulation of the radial nerve. As frequency of stimulation is increased, the amplitude of the primary decreases but remains constant at a given frequency. The evoked positive and negative phases of the primary usually disappear at frequencies below 30/sec. Unit responses, however, can still be found in a region where the primary is absent. As frequency of stimulation is raised, the unit responses begin to fail intermittently with complete disappearance of the spikes at about 60/sec. but firing can be encountered at much higher frequencies. In some preparations intermittent unit firing occurs at rates of stimulation between 500 and 1000/sec. The relationship of these findings to cortical mapping is discussed. It is concluded that these data do not support the method of mapping which depends upon the use of the evoked slow potentials.

Relationship of oestrogen and progesterone and their oviductal receptors in laying and non-laying 5-year-old hens

1985 ◽  
Vol 106 (3) ◽  
pp. 343-348 ◽  
Author(s):  
W. C. Okulicz ◽  
D. J. Fournier ◽  
H. Esber ◽  
T. N. Fredrickson
Keyword(s):  
Progesterone Receptor ◽  
Plasma Levels ◽  
Oestrogen Receptor ◽  
Laying Hens ◽  
Reproductive Function ◽  
Egg Laying ◽  
Ovulatory Cycle ◽  
Relationship Of ◽  
The Relationship ◽  
Domestic Hen

ABSTRACT This study focuses on the relationship of ageing to reproductive function in the domestic hen. Reproductive function was assessed by egg-laying records of hens from the same flock of 4, 5 and 6 years of age. Egg laying decreased with advancing age and the percentage of non-laying hens increased. During an ovulatory cycle, plasma levels of oestrogen and progesterone (including the preovulatory surge) were similar in both the 5-year-old and 1-year-old laying hens. In both young and old non-laying hens, the preovulatory surge of progesterone was absent. Levels of oestrogen were similar for all hens, but the oestrogen-dependent oviduct was atrophied in non-laying 5-year-old hens. This reduction in oviductal size was correlated with reduced levels of magnal nuclear oestrogen receptor and cytosol progesterone receptor. These results suggest a refractoriness of the oviduct to oestrogen stimulation in the ageing hen. J. Endocr. (1985) 106, 343–348

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