scholarly journals Susceptibilities of lactoferrin and transferrin to myeloperoxidase-dependent loss of iron-binding capacity

1988 ◽  
Vol 250 (2) ◽  
pp. 613-616 ◽  
Author(s):  
C C Winterbourn ◽  
A L Molloy
Keyword(s):  
Reactive Oxygen Species ◽  
Binding Capacity ◽  
Reactive Oxygen ◽  
Oxidative Modification ◽  
Iron Binding ◽  
Oxygen Species ◽  
Protective Groups ◽  
The Difference ◽  
Iron Binding Capacity

Apolactoferrin and apotransferrin lost their ability to subsequently bind iron when exposed to an excess of either HOCl or myeloperoxidase plus H2O2 and Cl-. Apolactoferrin, however, was more resistant than apotransferrin. By oxidizing a mixture of the two proteins, then separating them by immunoprecipitation, the difference in susceptibility was shown to be due to the greater reactivity of transferrin iron-binding groups, rather than protective groups on the lactoferrin molecule. The iron-saturated proteins were much more resistant to oxidative modification than the apoproteins. The greater resistance of apolactoferrin should be advantageous for maintaining its iron binding capacity when co-released with myeloperoxidase and reactive oxygen species from stimulated neutrophils.

Rescue of anaemia and autoimmune responses in SOD1-deficient mice by transgenic expression of human SOD1 in erythrocytes

2009 ◽  
Vol 422 (2) ◽  
pp. 313-320 ◽  
Author(s):  
Yoshihito Iuchi ◽  
Futoshi Okada ◽  
Rina Takamiya ◽  
Noriko Kibe ◽  
Satoshi Tsunoda ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Energy Charge ◽  
Reactive Oxygen ◽  
Oxidative Modification ◽  
Autoimmune Response ◽  
Oxygen Species ◽  
Autoantibody Production ◽  
Deficient Mice ◽  
Human Sod1

Oxidative stress has been implicated as a cause of various diseases such as anaemia. We found that the SOD1 [Cu,Zn-SOD (superoxide dismutase)] gene deficiency causes anaemia, the production of autoantibodies against RBCs (red blood cells) and renal damage. In the present study, to further understand the role of oxidative stress in the autoimmune response triggered by SOD1 deficiency, we generated mice that had the hSOD1 (human SOD1) transgene under regulation of the GATA-1 promoter, and bred the transgene onto the SOD1−/− background (SOD1−/−;hSOD1tg/+). The lifespan of RBCs, levels of intracellular reactive oxygen species, and RBC content in SOD1−/−;hSOD1tg/+ mice, were approximately equivalent to those of SOD1+/+ mice. The production of antibodies against lipid peroxidation products, 4-hydroxy-2-nonenal and acrolein, as well as autoantibodies against RBCs and carbonic anhydrase II were elevated in the SOD1−/− mice, but were suppressed in the SOD1−/−;hSOD1tg/+ mice. Renal function, as judged by blood urea nitrogen, was improved in the transgenic mice. These results rule out the involvement of a defective immune system in the autoimmune response of SOD1-deficient mice, because SOD1−/−;hSOD1tg/+ mice carry the hSOD1 protein only in RBCs. Metabolomic analysis indicated a shift in glucose metabolism to the pentose phosphate pathway and a decrease in the energy charge potential of RBCs in SOD1-deficient mice. We conclude that the increase in reactive oxygen species due to SOD1 deficiency accelerates RBC destruction by affecting carbon metabolism and increasing oxidative modification of lipids and proteins. The resulting oxidation products are antigenic and, consequently, trigger autoantibody production, leading to autoimmune responses.

scholarly journals Development and testing of an online method to measure ambient fine particulate reactive oxygen species (ROS) based on the 2',7'-dichlorofluorescin (DCFH) assay

2013 ◽  
Vol 6 (7) ◽  
pp. 1647-1658 ◽  
Author(s):  
L. E. King ◽  
R. J. Weber
Keyword(s):  
Reactive Oxygen Species ◽  
Limit Of Detection ◽  
Reactive Oxygen ◽  
Rural Site ◽  
Oxygen Species ◽  
Optimal Method ◽  
The Difference ◽  
Mist Chamber ◽  
Filter Particle ◽  
Urban Sites

Abstract. An online, semi-continuous instrument to measure fine particle (PM2.5) reactive oxygen species (ROS) was developed based on the fluorescent probe 2'7'-dichlorofluorescin (DCFH). Parameters that influence probe response were first characterized to develop an optimal method for use in a field instrument. The online method used a mist chamber scrubber to collect total (gas plus particle) ROS components (ROSt) alternating with gas phase ROS (ROSg) by means of an inline filter. Particle phase ROS (ROSp) was determined by the difference between ROSt and ROSg. The instrument was deployed in urban Atlanta, Georgia, USA, and at a rural site during various seasons. Concentrations from the online instrument generally agreed well with those from an intensive filter measurement of ROSp. Concentrations of the ROSp measurements made with this instrument were lower than reported in other studies, often below the instrument's average limit of detection (0.15 nmol H2O2 equivalents m−3). Mean ROSp concentrations were 0.26 nmol H2O2 equivalents m−3 at the Atlanta urban sites compared to 0.14 nmol H2O2 equivalents m−3 at the rural site.

THE CONTENT OF SH-GROUPS IN PLASMA OF PERIPHERAL BLOOD OF SHEEP EXPOSED TO CHRONIC INTAKE OF LEAD FROM DIET

2018 ◽  
Vol 1 (1) ◽  
pp. 106-109
Author(s):  
E. B. Mirzoev ◽  
◽  
V. O. Kobyalko ◽  
O. A. Gubina ◽  
N. A. Frolova ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Blood Plasma ◽  
Peripheral Blood ◽  
Reactive Oxygen ◽  
Oxidative Modification ◽  
Oxygen Species ◽  
Oxidative Modification Of Proteins ◽  
Sh Groups ◽  
Peripheral Blood Plasma

We studied the content of the SH-groups in plasma of peripheral blood of sheep, which during 90 days received the nitrate of lead in the diet at concentrations of 5 (1 MRL), 25 (5 MRL) and 150 mg/kg of feed (30 MRL). It is shown that chronic intake of lead in the organism of the sheep leads to a decrease in content of SH-groups in peripheral blood plasma. The most pronounced changes were noted when the concentration of lead in the diet was 25 mg/kg (5 MRL) and 150 mg/kg feed (30 MRL). It is supposed that with increasing of concentration of lead in the organs and tissues of mammals are initiated the processes of formation of reactive oxygen species that lead to oxidative modification of proteins and lipids.

scholarly journals Reactive Oxygen Species Registration in Planarian Regeneration

2015 ◽  
Vol 7 (6) ◽  
pp. 13 ◽  
Author(s):  
Kh. P. Tiras ◽  
S. V. Gudkov ◽  
V. I. Emelyanenko ◽  
K. B. Aslanidi
Keyword(s):  
Reactive Oxygen Species ◽  
Physiological State ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Planarian Regeneration ◽  
The Difference ◽  
Dying Cells ◽  
First Time ◽  
Kinetics Of

<p class="1Body">Reactive oxygen species (ROS) are directly involved in cell proliferation, differentiation and apoptosis in a variety of organisms. We studied kinetics of own luminescence induced by changes of ROS in early stages of planarian regeneration. Kinetics of chemiluminescence were measured in intact planarians and the same individuals after decapitation within 15 hours. We analyzed the traumatic fluorescent signal obtained as the difference between kinetics of intact and decapitated planarians. It was found that regeneration is accompanied by changes in the content of ROS correlated with the energy-intensive process in regenerating planarians. Oxidative stress was caused by damage to cell membranes in the dissection of the planarian and it was accompanied by a drop in the intensity of luminescence with a time constant of about 3.6 hours. Phagocytosis of dying cells by neoblasts was accompanied by an increase of the luminescence intensity after 2 - 3 hours after decapitation. Neoblast mitosis was described by two maximums of luminescence over 5.1 hours and 8.3 hours after decapitation. For the first time we demonstrated the opportunity of registering the physiological state of pluripotent stem cells at the level of the organism <em>in vivo</em>.</p>

Why have cells selected reactive oxygen species to regulate cell signaling events?

2002 ◽  
Vol 21 (2) ◽  
pp. 83-83 ◽  
Author(s):  
E R Stadtman ◽  
R L Levine
Keyword(s):  
Reactive Oxygen Species ◽  
Nucleic Acids ◽  
Cell Signaling ◽  
Signaling Pathways ◽  
Reactive Oxygen ◽  
Oxidative Modification ◽  
Oxygen Species ◽  
Growing Body ◽  
Signaling Events ◽  
Cell Signaling Pathways

There is a growing body of evidence demonstrating that exposure of cells to reactive oxygen species (ROS) leads to oxidative modification of nucleic acids, proteins, and lipids, and that such modifications can contribute to the development of a number of diseases and aging. This raises the question: If ROS are so damaging to cells, why have cells selected ROS to trigger activation of so many cell signaling pathways?

Modelling Copper Binding to the Amyloid-β Peptide in Alzheimer

2010 ◽  
Vol 63 (3) ◽  
pp. 345 ◽  
Author(s):  
V. Chandana Epa ◽  
Victor A. Streltsov ◽  
Joseph N. Varghese
Keyword(s):  
Reactive Oxygen Species ◽  
Binding Site ◽  
Reactive Oxygen Species Generation ◽  
Computational Modelling ◽  
Amyloid Β ◽  
Reactive Oxygen ◽  
Oxidative Modification ◽  
Amyloid Β Peptide ◽  
Oxygen Species ◽  
Copper Binding

Oxidative modification due to reactive oxygen species generated by Cu2+ bound to the amyloid-β peptide may be one of the sources of neurodegeneration observed in Alzheimer’s disease. Understanding the structure and function of the copper binding site can assist in the design of effective therapeutics. This paper highlights some of the most significant recent developments in computational modelling studies of the structure of the binding site and reaction mechanisms of reactive oxygen species generation.

scholarly journals Budding yeast Saccharomyces cerevisiae as a model to study oxidative modification of proteins in eukaryotes.

2006 ◽  
Vol 53 (4) ◽  
pp. 679-684 ◽  
Author(s):  
Volodymyr I Lushchak
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Reactive Oxygen Species ◽  
Protein Modification ◽  
Budding Yeast ◽  
Reactive Oxygen ◽  
Point Of View ◽  
Oxidative Modification ◽  
Oxygen Species ◽  
Yeast Saccharomyces Cerevisiae ◽  
Oxidative Protein Modification

The budding yeast Saccharomyces cerevisiae is a well studied unicellular eukaryotic organism the genome of which has been sequenced. The use of yeast in many commercial systems makes its investigation important not only from basic, but also from practical point of view. Yeast may be grown under both aerobic and anaerobic conditions. The investigation of the response of eukaryotes to different kinds of stresses was pioneered owing to yeast and here we focus mainly on the so-called oxidative stress. It is a result of an imbalance between the formation and decomposition of reactive oxygen species increasing their steady-state concentration. Reactive oxygen species may attack any cellular component. In the present review oxidation of proteins in S. cerevisiae is analyzed. There are two connected approaches to study oxidative protein modification - characterization of the overall process and identification of individual oxidized proteins. Because all aerobic organisms possess special systems which defend them against reactive oxygen species, the involvement of so-called antioxidant enzymes, particularly superoxide dismutase and catalase, in the protection of proteins is also analyzed.

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