scholarly journals Isolation and structural characterization of insulin and glucagon from the holocephalan species Callorhynchus milii (elephantfish)

1989 ◽  
Vol 263 (1) ◽  
pp. 261-266 ◽  
Author(s):  
B C Berks ◽  
C J Marshall ◽  
A Carne ◽  
S M Galloway ◽  
J F Cutfield
Keyword(s):  
Gas Phase ◽  
Receptor Binding ◽  
Structural Characterization ◽  
Sequence Similarity ◽  
Cartilaginous Fish ◽  
Proteolytic Processing ◽  
Reverse Phase ◽  
High Sequence Similarity ◽  
Processing Mechanisms

Both insulin and glucagon from the pancreas of the holocephalan cartilaginous fish Callorhynchus milii (elephantfish) have been isolated and purified. Two reverse-phase h.p.l.c. steps enabled recovery of sufficient material for gas-phase sequencing of the intact chains as well as peptide digestion products. The elephantfish insulin sequence shows 14 differences from pig insulin, including two unusual substitutions, Val-A14 and Gln-B30, though none of these is thought likely to influence receptor binding significantly. The insulin B-chain contains 31 residues, one more than mammalian insulins, but markedly less than that of the closely related ratfish with which it otherwise exhibits high sequence similarity. Elephantfish and pig glucagons differ at only four positions, but there are six changes from the ratfish glucagon-36 (normal glucagon contains 29 residues) sequence. It is apparent that different prohormone proteolytic processing mechanisms operate in the two holocephalan species.

scholarly journals Structural characterization of holo- and apo-myoglobin in the gas phase by ultraviolet photodissociation mass spectrometry

2015 ◽  
Vol 6 (2) ◽  
pp. 1324-1333 ◽  
Author(s):  
Michael B. Cammarata ◽  
Jennifer S. Brodbelt
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Structural Characterization ◽  
Ultraviolet Photodissociation ◽  
193 Nm

193 nm UV photodissociation of myoglobin in the gas phase showed preferential backbone cleavages in regions with higher relative B-factors.

scholarly journals Structural characterization of nucleotide 5′-triphosphates by infrared ion spectroscopy and theoretical studies

2018 ◽  
Vol 20 (44) ◽  
pp. 28319-28330 ◽  
Author(s):  
Rianne E. van Outersterp ◽  
Jonathan Martens ◽  
Giel Berden ◽  
Jeffrey D. Steill ◽  
Jos Oomens ◽  
...  
Keyword(s):  
Gas Phase ◽  
Structural Properties ◽  
Structural Characterization ◽  
Spectroscopic Studies ◽  
Theoretical Studies ◽  
Ion Spectroscopy ◽  
Ir Spectroscopic

Gas-phase IR spectroscopic studies and supporting theoretical studies have been performed on adenosine 5′-triphosphate, cytosine 5′-triphosphate and guanosine 5′-triphosphate to elucidate the structural properties of the NTPs by focusing on the influence of the nucleobase and the extent of deprotonation.

Structural Characterization of 3C-SiC Grown Using Methyltrichlorosilane

2013 ◽  
Vol 740-742 ◽  
pp. 291-294 ◽  
Author(s):  
Matteo Bosi ◽  
Giovanni Attolini ◽  
Béla Pécz ◽  
Zsolt Zolnai ◽  
László Dobos ◽  
...  
Keyword(s):  
Residual Stress ◽  
Gas Phase ◽  
Structural Characterization ◽  
Amorphous Content ◽  
Si Substrates

3C-SiC layers were grown on Si substrates using standard precursors (SiH4and C3H8) and by adding methyl trichloro silane (MTS) to the gas phase, with growth temperatures between 1200 and 1300 °C. Characterization of the 3C-SiC layers shows that 3C-SiC grown with MTS has higher polycrystalline and amorphous content as well as lower residual stress.

scholarly journals Tuning the ion selectivity of two-pore channels

2017 ◽  
Vol 114 (5) ◽  
pp. 1009-1014 ◽  
Author(s):  
Jiangtao Guo ◽  
Weizhong Zeng ◽  
Youxing Jiang
Keyword(s):  
Sequence Similarity ◽  
Ion Selectivity ◽  
Structural Basis ◽  
Monovalent Cations ◽  
Nonselective Cation Channel ◽  
High Sequence Similarity ◽  
Selective Channel ◽  
Tm Domain ◽  
Key Residues

Organellar two-pore channels (TPCs) contain two copies of aShaker-like six-transmembrane (6-TM) domain in each subunit and are ubiquitously expressed in plants and animals. Interestingly, plant and animal TPCs share high sequence similarity in the filter region, yet exhibit drastically different ion selectivity. Plant TPC1 functions as a nonselective cation channel on the vacuole membrane, whereas mammalian TPC channels have been shown to be endo/lysosomal Na+-selective or Ca2+-release channels. In this study, we performed systematic characterization of the ion selectivity of TPC1 fromArabidopsis thaliana(AtTPC1) and compared its selectivity with the selectivity of human TPC2 (HsTPC2). We demonstrate that AtTPC1 is selective for Ca2+over Na+, but nonselective among monovalent cations (Li+, Na+, and K+). Our results also confirm that HsTPC2 is a Na+-selective channel activated by phosphatidylinositol 3,5-bisphosphate. Guided by our recent structure of AtTPC1, we converted AtTPC1 to a Na+-selective channel by mimicking the selectivity filter of HsTPC2 and identified key residues in the TPC filters that differentiate the selectivity between AtTPC1 and HsTPC2. Furthermore, the structure of the Na+-selective AtTPC1 mutant elucidates the structural basis for Na+selectivity in mammalian TPCs.

a-SiC:H Doped with Reactive Gases and with Ion Implantation

1991 ◽  
Vol 219 ◽  
Author(s):  
F. Demichelis ◽  
C. F. Pirri ◽  
E. Tresso ◽  
G. Della Mea ◽  
V. Rigato ◽  
...  
Keyword(s):  
Ion Implantation ◽  
Gas Phase ◽  
Structural Characterization ◽  
Film Growth ◽  
Implantation Technique ◽  
Boron Doped ◽  
Order Of Magnitude ◽  
Compositional Disorder ◽  
Reactive Gases

ABSTRACTBoron doped a-SiC:H samples have been obtained both by gas phase doping during film growth and by using ion implantation. All the implanted samples were annealed under vacuum to remove the damage introduced by ion implantation and to produce a dopant diffusion. Physical properties deduced by optical, electrical and structural characterization of the two sets of samples have been compared. Ion implantation technique allows a better control of the dopant dose but increases the compositional disorder and the obtained conductivity values are one order of magnitude lower than those of gas doped samples.

scholarly journals Inheritance of seedlessness and the molecular characterization of the INO gene in Annonaceae

2023 ◽  
Vol 83 ◽  
Author(s):  
B. R. R. M. Nassau ◽  
P. S. C. Mascarenhas ◽  
A. G. Guimarães ◽  
F. M. Feitosa ◽  
H. M. Ferreira ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Type A ◽  
Molecular Techniques ◽  
Wild Type ◽  
Annona Squamosa ◽  
High Sequence Similarity ◽  
Breeding Programs ◽  
Complete Dominance ◽  
Young Leaves

Abstract The inheritance of the seedless fruit characteristic of Annona squamosa has not yet been explained. Molecular techniques may aid breeding programs, mainly in the assisted selection of the target gene. The INO gene may be related to seed development in these fruits. The objective of the present paper was to investigate the inheritance of seedlessness in the 'Brazilian seedless' sugar apple and INO gene conservation in Annona squamosa and Annona cherimola x Annona squamosa genotypes by assessing their homology with the INO database genes. The F1 generation was obtained by crossing the mutant 'Brazilian seedless' (male genitor) (P1) with the wild-type A. squamosa with seeds (M1 and M2, female genitors). The INO gene was studied in mutant and wild-type A. squamosa (P1, M1, M2 and M3) and in the Gefner atemoya (A. cherimola x A. squamosa) (M4) cultivar. The DNA was extracted from young leaves, and four sets of specific primers flanking the INO gene were amplified. The seedless characteristic was identified as stenospermatic in the fruits of parental P1, suggesting monogenic inheritance with complete dominance. High sequence similarity of the INO gene amplifications in the sugar apple accessions (M1, M2, M3) and the atemoya cultivar Gefner (M4) reinforces the hypothesis of their conservation.

scholarly journals Correction: Structural characterization of gas-phase cysteine and cysteine methyl ester complexes with zinc and cadmium dications by infrared multiple photon dissociation spectroscopy

2017 ◽  
Vol 19 (28) ◽  
pp. 18777-18778
Author(s):  
Rebecca A. Coates ◽  
Christopher P. McNary ◽  
Georgia C. Boles ◽  
Giel Berden ◽  
Jos Oomens ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Gas Phase ◽  
Structural Characterization ◽  
Chem Phys ◽  
Infrared Multiple Photon Dissociation ◽  
Phys Chem Chem Phys

Correction for ‘Structural characterization of gas-phase cysteine and cysteine methyl ester complexes with zinc and cadmium dications by infrared multiple photon dissociation spectroscopy’ by Rebecca A. Coates et al., Phys. Chem. Chem. Phys., 2015, 17, 25799–25808.

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