Let there be light: how to use photoswitchable cross-linker to reprogram proteins
2017 ◽
Vol 45
(3)
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pp. 831-837
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Keyword(s):
Azobenzene is a photo-isomerizing molecule whose end-to-end distance changes upon external illumination. When combined with site-specific reactive groups, it can be used as molecular tweezers to remote-control the structure and function of protein targets. The present study gives a brief overview over the rational design strategies that use an azobenzene-based photoswitchable cross-linker to engineer ON/OFF switches into functional proteins or to reprogram proteins for novel functions. The re-engineered proteins may be used as remote controls for cellular pathways, as light-gated drug delivery platforms or as light-powered machinery of synthetic cells and micro-scaled factories.
2014 ◽
Vol 19
(7)
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pp. 1000-1013
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2015 ◽
Vol 112
(10)
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pp. 2996-3001
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2016 ◽
Vol 12
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pp. 125-138
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2012 ◽
Vol 48
(1)
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pp. 1-16
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