Selective inhibition of PTEN preserves ischaemic post-conditioning cardioprotection in STZ-induced Type 1 diabetic rats: role of the PI3K/Akt and JAK2/STAT3 pathways

2016 ◽  
Vol 130 (5) ◽  
pp. 377-392 ◽  
Author(s):  
Rui Xue ◽  
Shaoqing Lei ◽  
Zhong-yuan Xia ◽  
Yang Wu ◽  
Qingtao Meng ◽  
...  
Keyword(s):  
Selective Inhibition ◽  
Diabetic Rats ◽  
Signalling Pathway ◽  
Pten Inhibition ◽  
Stat3 Signalling

The excessive expression of PTEN in diabetes contributes to the abolishment of cardioprotection by ischaemic post-conditioning. PTEN inhibition restores ischaemic post-conditioning-induced cardioprotection in diabetes mainly through up-regulating the PI3K/Akt signalling pathway and partially by up-regulating the JAK2/STAT3 signalling pathway.

scholarly journals AMPK activation restores ischemic post-conditioning cardioprotection in STZ-induced type 1 diabetic rats: Role of autophagy

2017 ◽  
Vol 16 (3) ◽  
pp. 3648-3656 ◽  
Author(s):  
Bin Zhou ◽  
Yan Leng ◽  
Shao-Qing Lei ◽  
Zhong-Yuan Xia
Keyword(s):  
Diabetic Rats ◽  
Ampk Activation

scholarly journals A Protective Role of Arecoline Hydrobromide in Experimentally Induced Male Diabetic Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Indraneel Saha ◽  
Joydeep Das ◽  
Biswaranjan Maiti ◽  
Urmi Chatterji
Keyword(s):  
Glucose Transporter ◽  
Serum Insulin ◽  
Diabetic Rats ◽  
Receptor Expression ◽  
Cell Regeneration ◽  
Sialic Acid Content ◽  
Glucose Transporter 2 ◽  
Experimentally Induced

Objectives.Arecoline, the most potent and abundant alkaloid of betel nut, causes elevation of serum testosterone and androgen receptor expression in rat prostate, in addition to increase in serum insulin levels in rats, leading to insulin resistance and type 2 diabetes-like conditions. This study investigated the role of arecoline on the reproductive status of experimentally induced type 1 diabetic rats.Methods.Changes in the cellular architecture were analyzed by transmission electron microscopy. Blood glucose, serum insulin, testosterone, FSH, and LH were assayed. Fructose content of the coagulating gland and sialic acid content of the seminal vesicles were also analyzed.Results.Arecoline treatment for 10 days at a dose of 10 mg/kg of body weight markedly facilitatedβ-cell regeneration and reversed testicular and sex accessory dysfunctions by increasing the levels of serum insulin and gonadotropins in type 1 diabetic rats. Critical genes related toβ-cell regeneration, such as pancreatic and duodenal homeobox 1 (pdx-1) and glucose transporter 2 (GLUT-2), were found to be activated by arecoline at the protein level.Conclusion.It can thus be suggested that arecoline is effective in ameliorating the detrimental effects caused by insulin deficiency on gonadal and male sex accessories in rats with type 1 diabetes.

scholarly journals Novel role of STAT3 in microglia-dependent neuroinflammation after experimental subarachnoid haemorrhage

2021 ◽  
pp. svn-2021-001028
Author(s):  
Zhiyuan Vera Zheng ◽  
Junfan Chen ◽  
Hao Lyu ◽  
Sin Yu Erica Lam ◽  
Gang Lu ◽  
...  
Keyword(s):  
Subarachnoid Haemorrhage ◽  
Inflammatory Responses ◽  
Morphological Changes ◽  
Neuronal Loss ◽  
Signalling Pathway ◽  
Janus Kinase ◽  
Signal Transducer ◽  
Experimental Subarachnoid Haemorrhage ◽  
Stat3 Signalling

Background and purposeSignal transducer and activator of transcription 3 (STAT3) may contribute to the proinflammation in the central nervous system diseases by modulating the microglial responses. Thus, this study was intended to investigate the effect of STAT3 on microglia-dependent neuroinflammation and functional outcome after experimental subarachnoid haemorrhage (SAH).MethodsThe SAH model was established by endovascular perforation in the mouse. Real-time PCR (RtPCR) and western blot were used to examine the dynamic STAT3 signalling pathway responses after SAH. To clarify the role of the STAT3 signalling pathway in the microglia-dependent neuroinflammation after SAH, the microglia-specific STAT3 knockout (KO) mice were generated by the Cre-LoxP system. The neurological functions were assessed by Catwalk and Morris water maze tests. Neuronal loss after SAH was determined by immunohistochemistry staining. Microglial polarisation status after STAT3 KO was then examined by RtPCR and immunofluorescence.ResultsThe STAT3 and Janus kinase-signal transducer 2 activated immediately with the upregulation and phosphorylation after SAH. Downstream factors and related mediators altered dynamically and accordingly. Microglial STAT3 deletion ameliorated the neurological impairment and alleviated the early neuronal loss after SAH. To investigate the underlying mechanism, we examined the microglial reaction after STAT3 KO. STAT3 deletion reversed the increase of microglia after SAH. Loss of STAT3 triggered the early morphological changes of microglia and primed microglia from M1 to M2 polarisation. Functionally, microglial STAT3 deletion suppressed the SAH-induced proinflammation and promoted the anti-inflammation in the early phase.ConclusionsSTAT3 is closely related to the microglial polarisation transition and modulation of microglia-dependent neuroinflammation. Microglial STAT3 deletion improved neurological function and neuronal survival probably through promoting M2 polarisation and anti-inflammatory responses after SAH. STAT3 may serve as a promising therapeutic target to alleviate early brain injury after SAH.

scholarly journals The localization of HPV and CMV in the adipose tissues of female diabetic type 1 rats and the possibility of having a role of reactivity of COVID-19 in diabetic subjects as a new medical hypothesis

2020 ◽  
Vol 10 (3) ◽  
pp. 71-73
Author(s):  
Ahed J Alkhatib
Keyword(s):  
Adipose Tissue ◽  
Synergistic Effects ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Obesity And Diabetes ◽  
Diabetic Model ◽  
Medical Hypothesis ◽  
The Impact

Introduction: Diabetes has various impacts on human body. It is thought that diabetes is predisposed by obesity. Obesity may due to several factors including genetically-environmental factors. The recent views that viruses may act as etiology for obesity. Study objectives: The main objectives of the present study were to investigate the possibility that CMV and HPV of having a role in initiating episodes of obesity and diabetes, and to test the hypothesis that co-existence of multi-viruses including corona virus may work synergistically to increase the impact of COVID-19 on diabetic patients. Methodology: In this study, a diabetic model was induced, the localization of HPV and CMV was determined using immunohistochemistry. Results: Study findings showed that both viruses HPV and CMV exist in the adipose tissue of diabetic rats. Both viruses were brown in color. Conclusions: Taken together, both CMV and HPV exist in the adipose tissue of diabetic rats, and this may explain the phenomenon of autoimmunity in diabetes from one side and from another side, we may explain the occurrence of synergistic effects of COVID-19 virus and the other viruses mentioned in this study.

scholarly journals Cytokine and Adhesion Molecule Expression Induced by Different Strains of Staphylococcus aureus in Type 1 Diabetic Rats: Role of Insulin

2019 ◽  
Vol 9 ◽  
Author(s):  
Paula R. Knox de Souza ◽  
Sabrina S. Ferreira ◽  
Fernanda P. B. Nunes ◽  
Felipe B. Casagrande ◽  
Fernando H. G. Tessaro ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Adhesion Molecule ◽  
Diabetic Rats ◽  
Adhesion Molecule Expression ◽  
Different Strains

scholarly journals Effects of Cholecalciferol on Key Components of Vitamin D-Endo/Para/Autocrine System in Experimental Type 1 Diabetes

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Anna Mazanova ◽  
Ihor Shymanskyi ◽  
Olha Lisakovska ◽  
Lala Hajiyeva ◽  
Yulia Komisarenko ◽  
...  
Keyword(s):  
Vitamin D ◽  
Type 1 Diabetes ◽  
Vitamin D Deficiency ◽  
Diabetic Rats ◽  
Hydroxyvitamin D ◽  
Male Wistar Rats ◽  
Experimental Type ◽  
25 Hydroxyvitamin D

Objectives.Recent prospective studies have found the associations between type 1 diabetes (T1D) and vitamin D deficiency. We investigated the role of vitamin D in the regulation of 25OHD-1α-hydroxylase (CYP27B1) and VDR expression in different tissues of T1D rats.Design.T1D was induced in male Wistar rats by streptozotocin (55 mg/k b.w.). After 2 weeks of T1D, the animals were treated orally with or without vitamin D3(cholecalciferol; 100 IU/rat, 30 days).Methods.Serum 25-hydroxyvitamin D (25OHD) was detected by ELISA. CYP27A1, CYP2R1, CYP27B1, and VDR were assayed by RT-qPCR and Western blotting or visualized by immunofluorescence staining.Results.We demonstrated that T1D led to a decrease in blood 25OHD, which is probably due to the established downregulation of CYP27A1 and CYP2R1 expression. Vitamin D deficiency was accompanied by elevated synthesis of renal CYP27B1 and VDR. Conversely, CYP27B1 and VDR expression decreased in the liver, bone tissue, and bone marrow. Cholecalciferol administration countered the impairments of the vitamin D-endo/para/autocrine system in the kidneys and extrarenal tissues of diabetic rats.Conclusions.T1D-induced vitamin D deficiency is associated with impairments of renal and extrarenal CYP27B1 and VDR expression. Cholecalciferol can be effective in the amelioration of diabetes-associated abnormalities in the vitamin D-endo/para/autocrine system.

scholarly journals Role of hyperglycaemia in the pathogenesis of hypotension observed in type-1 diabetic rats

2008 ◽  
Vol 89 (4) ◽  
pp. 292-300 ◽  
Author(s):  
I-Min Liu ◽  
Cheng Kuei Chang ◽  
Shiow-Wen Juang ◽  
Dai-Huang Kou ◽  
Yat-Ching Tong ◽  
...  
Keyword(s):  
Diabetic Rats
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