Characterization of two cDNAs that encode MAP kinase homologues in Arabidopsis thaliana and analysis of the possible role of auxin in activating such kinase activities in cultured cells

Abstract A 38-bp pollen-specific cis-acting region of the AVP1 gene is involved in the expression of the Arabidopsis thaliana V-PPase during pollen development. Here, we report the isolation and structural characterization of AtVOZ1 and AtVOZ2, novel transcription factors that bind to the 38-bp cis-acting region of A. thaliana V-PPase gene, AVP1. AtVOZ1 and AtVOZ2 show 53% amino acid sequence similarity. Homologs of AtVOZ1 and AtVOZ2 are found in various vascular plants as well as a moss, Physcomitrella patens. Promoter-β-glucuronidase reporter analysis shows that AtVOZ1 is specifically expressed in the phloem tissue and AtVOZ2 is strongly expressed in the root. In vivo transient effector-reporter analysis in A. thaliana suspension-cultured cells demonstrates that AtVOZ1 and AtVOZ2 function as transcriptional activators in the Arabidopsis cell. Two conserved regions termed Domain-A and Domain-B were identified from an alignment of AtVOZ proteins and their homologs of O. sativa and P. patens. AtVOZ2 binds as a dimer to the specific palindromic sequence, GCGTNx7ACGC, with Domain-B, which is comprised of a functional novel zinc coordinating motif and a conserved basic region. Domain-B is shown to function as both the DNA-binding and the dimerization domains of AtVOZ2. From highly the conservative nature among all identified VOZ proteins, we conclude that Domain-B is responsible for the DNA binding and dimerization of all VOZ-family proteins and designate it as the VOZ-domain.

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Characterization of the Sucrose Phosphate Phosphatase (SPP) Isoforms from Arabidopsis thaliana and Role of the S6PPc Domain in Dimerization

PLoS ONE ◽

10.1371/journal.pone.0166308 ◽

2016 ◽

Vol 11 (11) ◽

pp. e0166308 ◽

Cited By ~ 3

Author(s):

Tomás Albi ◽

M. Teresa Ruiz ◽

Pedro de los Reyes ◽

Federico Valverde ◽

José M. Romero

Keyword(s):

Arabidopsis Thaliana ◽

Sucrose Phosphate Phosphatase ◽

Sucrose Phosphate

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Characterization of factor-independent variants derived from TF-1 hematopoietic progenitor cells: the role of the Raf/MAP kinase pathway in the anti-apoptotic effect of GM-CSF

Oncogene ◽

10.1038/sj.onc.1200884 ◽

1997 ◽

Vol 14 (6) ◽

pp. 721-728 ◽

Cited By ~ 12

Author(s):

Jhy-Rong Chao ◽

Chyi-Shyan Chen ◽

Ting-Fang Wang ◽

Li-Hui Tseng ◽

Jaw-Ji Tsai ◽

...

Keyword(s):

Map Kinase ◽

Progenitor Cells ◽

Hematopoietic Progenitor Cells ◽

Hematopoietic Progenitor ◽

Gm Csf ◽

Apoptotic Effect ◽

Map Kinase Pathway ◽

Kinase Pathway

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Cloning and Characterization of a Flavonol Synthase Gene fromScutellaria baicalensis

The Scientific World JOURNAL ◽

10.1155/2014/980740 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 4

Author(s):

Yeon Bok Kim ◽

KwangSoo Kim ◽

YeJi Kim ◽

Pham Anh Tuan ◽

Haeng Hoon Kim ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Amino Acid ◽

Cdna Clone ◽

Amino Acid Residues ◽

Anthocyanidin Synthase ◽

Transcript Levels ◽

Flavonol Synthase ◽

Jelly Roll

Flavonols are the most abundant of all the flavonoids and play pivotal roles in a variety of plants. We isolated a cDNA clone encoding flavonol synthase fromScutellaria baicalensis(SbFLS). The SbFLS cDNA is 1011 bp long, encodes 336 amino acid residues, and belongs to a family of 2-oxoglutarate-dependent dioxygenases. The overall structure ofSbFLSis very similar to that ofArabidopsis thalianaanthocyanidin synthase (AtANS), with aβjelly-roll fold surrounded by tens of short and longα-helices.SbFLSwas constitutively expressed in the roots, stems, leaves, and flowers, with particularly high expression in the roots and flowers. SbFLS transcript levels in the roots were 376-, 70-, and 2.5-fold higher than in the leaves, stems, and flowers. The myricetin content was significantly higher than that of kaempferol and quercetin. Therefore, we suggest that SbFLS mediates flavonol formation in the different organs ofS. baicalensis. Our study may contribute to the knowledge of the role of FLS inS. baicalensis.

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Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants

Plant Growth Regulation ◽

10.1007/s10725-010-9467-8 ◽

2010 ◽

Vol 61 (3) ◽

pp. 231-242 ◽

Cited By ~ 15

Author(s):

Somya Dwivedi ◽

Radomira Vanková ◽

Vaclav Motyka ◽

Carmen Herrera ◽

Eva Zizkova ◽

...

Keyword(s):

Arabidopsis Thaliana

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Differences in the Manifestation of Cell Pluripotence In Vivo and In Vitro in the Mutant Arabidopsis thaliana with the Phenotype of Cell Memory Disorder

Russian Journal of Plant Physiology ◽

10.1134/s1021443721010106 ◽

2021 ◽

Vol 68 (1) ◽

pp. 46-55

Author(s):

E. V. Kupriyanova ◽

E. R. Denisova ◽

M. A. Baier ◽

T. A. Ezhova

Keyword(s):

Arabidopsis Thaliana ◽

Shoot Regeneration ◽

Cultured Cells ◽

De Novo ◽

Epigenetic Memory ◽

In Planta ◽

Pluripotency Genes

Abstract Plant cells cultivated in vitro are a convenient model for studying the genetic and physiological mechanisms necessary for the cells to acquire a state of pluripotency. Earlier studies on a model plant Arabidopsis thaliana (L.) Heynh. have identified the key role of genes that determine the pluripotency of cells in the shoot apical meristem in de novo shoot regeneration in tissue culture. In accordance with this, cells of mutant plants with a higher level of expression of pluripotency genes were characterized by an increased potential for de novo shoot regeneration. The tae mutant was the exception to this rule. The mutant resumed the expression of pluripotency genes and cell proliferation at the late stages of leaf development, which indicates a violation of the mechanisms for maintaining epigenetic cellular memory. At the same time, leaf cells cultured in vitro showed a lower proliferative activity compared to the wild type and were not capable of de novo regeneration of shoots. A decrease in the regenerative potential of cultured cells of the tae mutant indicates an important role of epigenetic memory in the response of cells to exogenous hormones. Impaired epigenetic memory of leaf cells of the tae mutant and differences in their proliferative and regenerative capacities in planta and in vitro make this mutant a unique model for studying the role of epigenetic modifications in the regulation of cell pluripotency.

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Characterization of male-sterile Arabidopsis thaliana mutants exhibiting altered tapetum development

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100085344 ◽

1991 ◽

Vol 49 ◽

pp. 208-209

Author(s):

C. S. Bricker ◽

N. Smith-Huerta ◽

C. A. Makaroff

Keyword(s):

Arabidopsis Thaliana ◽

Male Sterility ◽

Insertional Mutagenesis ◽

Molecular Evidence ◽

Growth Substances ◽

Microspore Development ◽

Male Sterile ◽

Molecular Signals

The tapetum is the tissue in the anther that is in closest contact with the developing microspores. It consists of large and frequently multinucleate, richly cytoplasmic cells that provide most of the nutrients and growth substances necessary for the developing microspore. A great deal of our knowledge on the tapetum has come from studies on malesterile mutants. We are using T-DNA insertional mutants of Arabidopsis thaliana to identify, isolate and characterize the genes that control the differentiation of the tapetum. As a first step we have identified those mutants that exhibit the sporogenous type of male sterility (relatively normal stamen development but alterations in microsporogenesis). Further analyses have identified those mutants that exhibit alterations in tapetum development that have resulted from insertional mutagenesis by the T-DNA. Through the analysis of these mutants we hope to identify the molecular signals that regulate tapetal tissue differentiation and provide molecular evidence on the role of the tapetum in microspore development.

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Differential Expression of Fungal Genes Determines the Lifestyle of Plectosphaerella Strains During Arabidopsis thaliana Colonization

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-03-20-0057-r ◽

2020 ◽

Vol 33 (11) ◽

pp. 1299-1314 ◽

Cited By ~ 1

Author(s):

Antonio Muñoz-Barrios ◽

Sara Sopeña-Torres ◽

Brisa Ramos ◽

Gemma López ◽

Irene del Hierro ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

In Planta ◽

Necrotrophic Fungi ◽

Pathogenesis Related ◽

Nonpathogenic Strain ◽

Genes Encoding ◽

Fungal Genes ◽

Growth Adaptation

The fungal genus Plectosphaerella comprises species and strains with different lifestyles on plants, such as P. cucumerina, which has served as model for the characterization of Arabidopsis thaliana basal and nonhost resistance to necrotrophic fungi. We have sequenced, annotated, and compared the genomes and transcriptomes of three Plectosphaerella strains with different lifestyles on A. thaliana, namely, PcBMM, a natural pathogen of wild-type plants (Col-0), Pc2127, a nonpathogenic strain on Col-0 but pathogenic on the immunocompromised cyp79B2 cyp79B3 mutant, and P0831, which was isolated from a natural population of A. thaliana and is shown here to be nonpathogenic and to grow epiphytically on Col-0 and cyp79B2 cyp79B3 plants. The genomes of these Plectosphaerella strains are very similar and do not differ in the number of genes with pathogenesis-related functions, with the exception of secreted carbohydrate-active enzymes (CAZymes), which are up to five times more abundant in the pathogenic strain PcBMM. Analysis of the fungal transcriptomes in inoculated Col-0 and cyp79B2 cyp79B3 plants at initial colonization stages confirm the key role of secreted CAZymes in the necrotrophic interaction, since PcBMM expresses more genes encoding secreted CAZymes than Pc2127 and P0831. We also show that P0831 epiphytic growth on A. thaliana involves the transcription of specific repertoires of fungal genes, which might be necessary for epiphytic growth adaptation. Overall, these results suggest that in-planta expression of specific sets of fungal genes at early stages of colonization determine the diverse lifestyles and pathogenicity of Plectosphaerella strains.

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Characterization of the Binding of Diarrheagenic Strains of E. coli to Plant Surfaces and the Role of Curli in the Interaction of the Bacteria with Alfalfa Sprouts

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-18-1235 ◽

2005 ◽

Vol 18 (11) ◽

pp. 1235-1242 ◽

Cited By ~ 69

Author(s):

Cecelia Jeter ◽

Ann G. Matthysse

Keyword(s):

Escherichia Coli ◽

Arabidopsis Thaliana ◽

No Reduction ◽

E Coli ◽

Diarrheagenic Escherichia Coli ◽

Alfalfa Sprouts ◽

Plant Surfaces ◽

Seed Coats

Diarrheagenic Escherichia coli were able to bind to plant surfaces, including alfalfa sprouts and open seed coats, and tomato and Arabidopsis thaliana seedlings incubated in water. The characteristics of the binding differed with the bacterial strain examined. Laboratory K12 strains of E. coli failed to show significant binding to any of the plant surfaces examined, suggesting that some of the genes present and expressed in pathogenic strains and absent or unexpressed in K12 strains may be required for binding to plants. When a plasmid carrying the mlrA gene (a positive regulator of curli biosynthesis) or a plasmid carrying the operons that encode the synthesis of curli (csgA-G) was introduced into K12 strains, the bacteria acquired the ability to bind to sprouts. CsgA mutants of an avian pathogenic E. coli and an O157:H7 strain showed no reduction in their ability to bind to sprouts. Thus, the production of curli appears to be sufficient to allow K12 strains to bind, but curli are not necessary for the binding of pathogenic strains, suggesting that pathogenic strains may have more than one mechanism for binding to plant surfaces.

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