scholarly journals Pathogenetic substantiation of the combined transplantation use of multipotent mesenchymal stromal cells and hepatic stellate cells to restore the liver morphofunctional state after acute toxic hepatitis in the old body

2020 ◽  
Vol 22 ◽  
pp. 01009
Author(s):  
Irina Maklakova ◽  
Dmitry Grebnev ◽  
Victoria Vakhrusheva ◽  
Ilya Gavrilov
Keyword(s):  
Liver Regeneration ◽  
Toxic Hepatitis ◽  
Cell Types ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Laboratory Animals ◽  
Laboratory Mice ◽  
Toxic Damage ◽  
Acute Toxic Hepatitis ◽  
Old Mice ◽  
Acute Toxic

The purpose of this study was to study the cotransplantation influence of multipotent mesenchymal stromal (MMSC) and hepatic stellate (HSC) cells on liver regeneration of old laboratory animals in conditions of its toxic damage. Acute toxic hepatitis was caused by single intraperitoneal CC14 injection at a dose of 50 μg/kg. The introduction of MMSC and HSC was carried out at doses of 4 million cl/kg and 9 million cl/kg respectively 1 hour after toxic hepatitis modelling. The morphofunctional liver state of old laboratory mice was evaluated on the 1st, 3rd, 7th day after combined injection of MMSC and HSC in laboratory animals with toxic hepatitis. As a result of the study, it was obtained that MMSC and HSC cotransplantation leads to cellular and intracellular liver regeneration activation in old mice with acute toxic hepatitis. Also, the introduction of these cell types leads to decreased liver mutagenesis, inhibition of programmed cellular hepatocytes death. Thus, the conducted studies indicate the ability of combined MMSC and HSC transplantation to restore the morphofunctional liver state of the old organism under the conditions of its toxic damage.

scholarly journals Study of clustered DNA lesions in the liver in acute toxic injury

2020 ◽  
pp. 29-37
Author(s):  
A. G. Skuratov ◽  
A. S. Shaforost ◽  
A. A. Zyatskov ◽  
A. N. Lyzikov ◽  
O. V. Osipkina ◽  
...  
Keyword(s):  
Toxic Hepatitis ◽  
Dna Lesions ◽  
Primary Data ◽  
Control Group ◽  
Dna Integrity ◽  
Toxic Damage ◽  
Acute Toxic Hepatitis ◽  
Successful Testing ◽  
Toxic Liver Injury ◽  
Acute Toxic

Objective: to test and optimize a method for detecting clustered DNA lesions with an assessment of the quantitative characteristics of hepatic changes using an experimental model of acute toxic hepatitis.Material and methods: Laboratory C57Bl/6 mice at the age of 10 weeks were used for the study. Acute toxic liver injury was induced by means of a single intraperitoneal injection of 30 % CCI4 solution in olive oil. The withdrawal of the animals from the experiment was made after 72 hours. For the detection of clustered DNA lesions, a liver fragment was resected.Results. The electrophoretic parameters which are the most optimal for obtaining primary data for the subsequent calculation of the number of clustered DNA lesions have been proposed. The number of clusters in the DNA samples from the animals of the control group is significantly lower than in the experimental group and amounts to 54.80 [37.65; 59.24] and 76.82 [60.95; 92.41] APE1 clusters per million bp., respectively.Conclusion. Successful testing and optimization of the OCDL method for the detection of clustered lesions in liver DNA have been performed. The study has shown an increase in the number of APE1 clusters and double-strand breaks in the DNA of the C57Bl laboratory mice with induced acute toxic hepatitis, which indicates significant derangement of DNA integrity and a high risk of developing progressive liver diseases in its toxic damage.

Action of sodium aurothiomalate in acute toxic hepatitis

1977 ◽  
Vol 83 (5) ◽  
pp. 637-640
Author(s):  
T. A. Korolenko ◽  
V. A. Shkurupii ◽  
V. M. Subbotin ◽  
G. S. Yakobson
Keyword(s):  
Toxic Hepatitis ◽  
Sodium Aurothiomalate ◽  
Acute Toxic Hepatitis ◽  
Acute Toxic

Acute Toxic Hepatitis Induced by Herbal Medicine in Assam: A Pilot Study

2018 ◽  
Vol 113 (Supplement) ◽  
pp. S567 ◽  
Author(s):  
Bhabadev Goswami ◽  
Preeti Sharma ◽  
Snigdha Saikia ◽  
Prajjalendra Barooah ◽  
Mallika Bhattacharyya ◽  
...  
Keyword(s):  
Pilot Study ◽  
Herbal Medicine ◽  
Toxic Hepatitis ◽  
Acute Toxic Hepatitis ◽  
Acute Toxic

scholarly journals Acute Hepatitis after Ingestion of a Preparation of Chinese Skullcap and Black Catechu for Joint Pain

2016 ◽  
Vol 2016 ◽  
pp. 1-3 ◽  
Author(s):  
Charilaos Papafragkakis ◽  
Mel A. Ona ◽  
Madhavi Reddy ◽  
Sury Anand
Keyword(s):  
Side Effects ◽  
Acute Hepatitis ◽  
Joint Pain ◽  
Toxic Hepatitis ◽  
Herbal Preparations ◽  
Wide Range ◽  
Herbal Medications ◽  
Acute Toxic Hepatitis ◽  
Acute Toxic

Many herbal preparations are routinely used and have been occasionally associated with a wide range of side effects, from mild to severe. Chinese skullcap and black catechu are herbal medications commonly used for their hepatoprotective and other properties. We report a case of acute toxic hepatitis associated with ingestion of Chinese skullcap and black catechu in one preparation for the alleviation of joint pain.

Aloe vera-induced acute toxic hepatitis in a healthy young man

2006 ◽  
Vol 17 (8) ◽  
pp. 589 ◽  
Author(s):  
Ozkan Kanat ◽  
Ahmet Ozet ◽  
Selmin Ataergin
Keyword(s):  
Toxic Hepatitis ◽  
Aloe Vera ◽  
Acute Toxic Hepatitis ◽  
Acute Toxic

scholarly journals Possibility of using combined transplantation of multipotent mesenchymal stromal cells and hepatic stellate cells to activate reparative liver regeneration in mature and old organism

2020 ◽  
Vol 22 ◽  
pp. 01004
Author(s):  
Irina Maklakova ◽  
Dmitry Grebnev ◽  
Victoria Vakhrusheva ◽  
Ekaterina Petrunina
Keyword(s):  
Body Weight ◽  
Liver Regeneration ◽  
Cell Transplantation ◽  
Stromal Cells ◽  
Age Groups ◽  
Stellate Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Laboratory Animals ◽  
Subtotal Resection ◽  
Intracellular Regeneration

The aim of the study was to examine the effect of combined transplantation of multipotent mesenchymal stromal (MMSC) and hepatic stellate (HSC) cells on liver regeneration after resection. Research has been carried out on laboratory animals of mature and old age. After the subtotal resection of the liver, MMSC and HSC were introduced in the tail vein in the amount of 4 million cl/kg of body weight and 9 million cl/kg of body weight respectively. Evaluation of reparative liver regeneration was performed on the 1st, 3rd, 7th days after subtotal liver resection. Features of reparative liver regeneration in mature and old organism were revealed. In mature organism against the background of combined cell transplantation, regeneration activation is achieved by increasing cellular and intracellular regeneration mechanisms. In this case, the old organism responds to cell transplantation by activating only intracellular mechanisms. In both age groups, decreased mutagenesis and inhibition of programmed cell death against the background of MMSC cotransplantation and HSC were observed.

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