Identification of arbuscular mycorrhizal fungi in soils of the North Caucasus based on Illumina MiSeq data for ITS1 and ITS2 regions

The objective of our research was to analyze the efficiency of identification of arbuscular mycorrhizal fungi (AMF) for 2 regions: ITS1 and ITS2 regions of AMF DNA isolated from the soils of the North Caucasus (Karachay-Cherkessia). For the first time the necessity of different AMF species identification using both ITS regions was revealed, but not one region. The research demonstrated: 1) the set of taxa is different using ITS1- and ITS2-based identification; 2) analysis of the ITS1 region reveals a greater number of operational taxonomic units; 3) ITS2 allows identification of AMF at the species level more often. Sample preparation for Illumina MiSeq analysis was optimized. Obligatory stages in the sample preparation were the purification of DNA in the agarose gel in Silica after isolation, as well as separate amplification of ITS1 and ITS2 followed by combining and joint sequencing for each sample. The results showed the highest AMF biodiversity for the 176Te sample from the ecosystem of the subalpine meadow of the southeastern slope of Malaya Hatipara mountain (43°25′48.0″N 41°42′31.0″E; 2401 m above sea level), in which 8 species of AMF were identified (Archaeospora spainiae, Claroideoglomus claroideum, Diversispora versiformis, Entrophpora infrequens, Funneliformis mosseae, Glomus indicum, Paraglomus laccatum, Rhizophagus irregularis).

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Arbuscular mycorrhizal fungi of the North Caucasus and the Gorny Altai: biodiversity and role in plant-microbial interactions

Plant Genetics, Genomics, Bioinformatics, and Biotechnology (PlantGen2021) ◽

10.18699/plantgen2021-220 ◽

2021 ◽

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Arbuscular Mycorrhizal ◽

Microbial Interactions ◽

North Caucasus ◽

The North ◽

Gorny Altai

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Sporocarpic species of arbuscular mycorrhizal fungi (Glomeromycota), with a new report from Brazil

Acta Botanica Brasilica ◽

10.1590/s0102-33062005000300025 ◽

2005 ◽

Vol 19 (3) ◽

pp. 633-637 ◽

Cited By ~ 9

Author(s):

Bruno Tomio Goto ◽

Leonor Costa Maia

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Atlantic Forest ◽

Mycorrhizal Fungi ◽

Arbuscular Mycorrhizal ◽

Northeast Brazil ◽

Natural Areas ◽

Agricultural Ecosystems ◽

Brazilian Soils ◽

First Time

The arbuscular mycorrhizal fungi (AMF) are widely distributed in terrestrial ecossystems; however the sporocarpic species are less documented on AMF surveys. Five of these species were found in natural areas of Atlantic Forest and in agricultural ecosystems of Northeast Brazil: Glomus glomerulatum, G. sinuosum, G. coremioides, G. fuegianum and G. taiwanensis, with the last one being registered for the first time in Brazilian soils.

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Dynamics of arbuscular mycorrhizal fungi in relation to root colonization, spore density, and soil properties among different spreading stages of the exotic plant threeflower beggarweed (Desmodium triflorum) in a Zoysia tenuifolia lawn

Weed Science ◽

10.1017/wsc.2019.50 ◽

2019 ◽

Vol 67 (6) ◽

pp. 689-701

Author(s):

Xiaoge Han ◽

Changchao Xu ◽

Yutao Wang ◽

Dan Huang ◽

Qiang Fan ◽

...

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Soil Properties ◽

Mycorrhizal Fungi ◽

Arbuscular Mycorrhizal ◽

Spore Density ◽

Invasive Weed ◽

Weed Invasion ◽

Operational Taxonomic Units ◽

Amf Communities ◽

Spore Densities

AbstractWeed invasion is a prevailing problem in modestly managed lawns. Less attention has been given to the exploration of the role of arbuscular mycorrhizal fungi (AMF) under different invasion pressures from lawn weeds. We conducted a four-season investigation into a Zoysia tenuifolia Willd. ex Thiele (native turfgrass)–threeflower beggarweed [Desmodium triflorum (L.) DC.] (invasive weed) co-occurring lawn. The root mycorrhizal colonizations of the two plants, the soil AM fungal communities and the spore densities under five different coverage levels of D. triflorum were investigated. Desmodium triflorum showed significantly higher root hyphal and vesicular colonizations than those of Z. tenuifolia, while the root colonizations of both species varied significantly among seasons. The increased coverage of D. triflorum resulted in the following effects: (1) the spore density initially correlated with mycorrhizal colonizations of Z. tenuifolia but gradually correlated with those of D. triflorum. (2) Correlations among soil properties, spore densities, and mycorrhizal colonizations were more pronounced in the higher coverage levels. (3) Soil AMF community compositions and relative abundances of AMF operational taxonomic units changed markedly in response to the increased invasion pressure. The results provide strong evidence that D. triflorum possessed a more intense AMF infection than Z. tenuifolia, thus giving rise to the altered host contributions to sporulation, soil AMF communities, relations of soil properties, spore densities, and root colonizations of the two plants, all of which are pivotal for the successful invasion of D. triflorum in lawns.

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Illumina-based identification of arbuscular mycorrhizal fungi from Karachay-Cherkessia soils for development of bio-fertilizers

E3S Web of Conferences ◽

10.1051/e3sconf/202128503001 ◽

2021 ◽

Vol 285 ◽

pp. 03001

Author(s):

Andrey Yurkov ◽

Alexey Kryukov ◽

Yulia Mikhaylova ◽

Peter Zhurbenko

Keyword(s):

Mycorrhizal Fungi ◽

Biological Diversity ◽

Arbuscular Mycorrhizal ◽

Its Region ◽

Am Fungi ◽

Illumina Miseq ◽

Universal Primers ◽

Its2 Region ◽

North Caucasus ◽

The Future

The aim of the study was to investigate the species diversity of AM fungi in different parts of the North Caucasus, biodiversity hotspot, the center of the world’s biological diversity. Samples were taken from 5 locations (stationary trial plots, STPs) in different ecosystems and at various altitudes. Identification was performed using sequencing for ITS1 and ITS2 regions, amplified with universal primers, Illumina MiSeq was employed. 19 genera of AM fungi were found on all STPs. The work did not reveal a correlation between the altitude and the species composition of AM fungi. At the same time, it should be assumed that a correlation could be found between the biodiversity of AM fungi and the type of ecosystem, which should be done in the future. The study shows it is necessary to use an analysis for both ITS regions, since the data obtained for each ITS region differ and complement each other. Analysis for the ITS2 region revealed 1.3 times more virtual taxa than for the ITS1, while the number of OTUs identified per species was similar for both regions. The highest biodiversity of AM fungi was found in STP #3 (with meadow flora). Only 4 species (Rhizophagus irregularis, R. intraradices, Paraglomus laccatum, and Claroideoglomus claroideum) were found on all five analyzed STPs. We found unexpectedly that with such a high biodiversity among the identified fungi, no different species were found in the Paraglomus genus, all the sequences of Paraglomus belonged to Paraglomus laccatum, whereas at least 9 species are distinguished in the genus by morphology. Further research will allow us to identify new strains of AM fungi, the efficiency of which may be higher than already studied ones. In the future this will make it possible to create more effective microbial biofertilizers for agriculture.

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Spatiotemporal differences in the arbuscular mycorrhizal fungi communities in soil and roots in response to long-term organic compost inputs in an intensive agricultural cropping system on the North China Plain

Journal of Soils and Sediments ◽

10.1007/s11368-019-02244-3 ◽

2019 ◽

Vol 19 (5) ◽

pp. 2520-2533 ◽

Cited By ~ 7

Author(s):

Wei Liu ◽

Yunlong Zhang ◽

Shanshan Jiang ◽

Philip J. Murray ◽

Liqin Liao ◽

...

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

North China Plain ◽

North China ◽

Cropping System ◽

Arbuscular Mycorrhizal ◽

The North ◽

The North China Plain ◽

Organic Compost

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The effect of amendments on Lolium perenne roots arbuscular mycorrhizal fungi colonization when cultivated in contaminated soil

International Journal of Environmental Science and Technology ◽

10.1007/s13762-021-03783-4 ◽

2021 ◽

Author(s):

A. Szada-Borzyszkowska ◽

J. Krzyżak ◽

S. Rusinowski ◽

A. Starzewska-Sikorska ◽

I. Ratman-Kłosińska ◽

...

Keyword(s):

Heavy Metals ◽

Arbuscular Mycorrhizal Fungi ◽

Lolium Perenne ◽

Contaminated Soil ◽

Mycorrhizal Fungi ◽

Arbuscular Mycorrhizal ◽

Spore Morphology ◽

Spoil Heap ◽

Dry Biomass ◽

First Time

AbstractArbuscular Mycorrhizal Fungi that colonize the roots of plants growing on lands contaminated by heavy metals may influence the phytostabilization process reducing the translocation of metals to the aboveground parts of the plant. This study aimed to evaluate the effects of soil amendments (lime and lignite) on the concentration of the bioavailable form of heavy metals (CaCl2 extraction) in soil and on the colonization of Arbuscular Mycorrhizal Fungi in the roots of Lolium perenne when cultivated in contaminated soil. During the experiment, the bioavailability of Pb, Cd, and Zn in soil was significantly reduced after application of the amendments, causing an increase of L. perenne shoot dry biomass. It was observed that the higher dose of lime (0.5%) resulting in amplified values of relative mycorrhizal intensity. However, independently of the dose, the treatments increased the occurrence of arbuscules in L. perenne roots, with the highest value observed after the application of 0.25% lime with 5% lignite. The results for the first time present the effect of lime and lignite application on the L. perenne roots colonization by Arbuscular Mycorrhizal Fungi indicating the increase of occurrence of arbuscules. These findings suggest that in order to explain the different responses of Arbuscular Mycorrhizal Fungi to the applied treatment further investigations are needed to identify the spore morphology. The results of the experiment were implemented to stabilize heavy metals during remediation of a spoil heap in Ruda Śląska, Poland.

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Diversity and distribution of Arbuscular Mycorrhizal Fungi in cassava (Manihot esculenta Crantz) croplands in Cameroon as revealed by Illumina MiSeq

Rhizosphere ◽

10.1016/j.rhisph.2019.100147 ◽

2019 ◽

Vol 10 ◽

pp. 100147 ◽

Cited By ~ 1

Author(s):

Papa Saliou Sarr ◽

Akifumi Sugiyama ◽

Aime Didier Boyogueno Begoude ◽

Kazufumi Yazaki ◽

Shigeru Araki ◽

...

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Manihot Esculenta ◽

Arbuscular Mycorrhizal ◽

Illumina Miseq ◽

Manihot Esculenta Crantz

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Mycorrhizal associations in Polylepis woodlands of Central Argentina

Canadian Journal of Botany ◽

10.1139/b07-042 ◽

2007 ◽

Vol 85 (5) ◽

pp. 526-531 ◽

Cited By ~ 15

Author(s):

Eugenia Menoyo ◽

Alejandra G. Becerra ◽

Daniel Renison

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Plant Species ◽

Mycorrhizal Fungi ◽

National Park ◽

Arbuscular Mycorrhizal ◽

Dark Septate Endophyte ◽

Central Argentina ◽

Mycorrhizal Associations ◽

Colonization Patterns ◽

First Time

Polylepis woodlands are one of the most threatened mountain ecosystems of South America, and their restoration is a high priority. To assess the mycorrhizal status in Polylepis woodlands of “Quebrada del Condorito” National Park (Córdoba Mountains, Central Argentina), we examined the roots of 22 plant species, belonging to 14 families and determined morphological types of arbuscular mycorrhiza (Arum and Paris type) and colonization level. The 22 species were colonized by arbuscular mycorrhizal fungi and dark septate endophytes. Different arbuscular mycorrhizal structures and colonization patterns were observed, although Paris-type colonization was predominant. Fourteen plant species are reported for the first time as hosts of arbuscular mycorrhizal fungi. We conclude that arbuscular mycorrhizal fungi and dark septate endophyte hosts are widespread in the Polylepis woodlands of Central Argentina and should receive special attention in future revegetation programs.

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Arbuscular mycorrhizal fungi of the Brda river valley in the Tuchola Forests

Acta Mycologica ◽

10.5586/am.2000.001 ◽

2014 ◽

Vol 35 (1) ◽

pp. 3-23 ◽

Cited By ~ 2

Author(s):

Mariusz Tadych ◽

Janusz Błaszkowski

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Plant Species ◽

Mycorrhizal Fungi ◽

Rhizosphere Soil ◽

Arbuscular Mycorrhizal ◽

River Valley ◽

The World ◽

First Time

The occurrence of arbuscular mycorrhizal fungi (AMF) associated with 19 plant species from 12 families growing in the Brda river valley in the Tuchola Forests is presented. The most frequently investigated plans were those from the families Cupressaceae and Plantaginaceae. Examination of trap cultures established from rhizosphere soil-root mixtures of the plans sampled revealed 20 described species of AMF, two undescribed Glomus spp., and three species of the genus Glomus that were difficult to recognize. The dominating AMF were members of the genus Glomus. Among the AMF found, G. claroideum was present, a species recorded for the first time in Poland. The distribution in Poland and in the world of the AMF encountered is shown.

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First report of enhanced contents of nine macro- and micronutrients in gymnosperms via arbuscular mycorrhizal fungi

10.1101/515031 ◽

2019 ◽

Author(s):

Alicia Franco ◽

Jesús Pérez-Moreno ◽

Gabriela Sánchez ◽

Carlos R. Cerdán ◽

Juan J. Almaraz ◽

...

Keyword(s):

Plant Growth ◽

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Arbuscular Mycorrhizae ◽

Arbuscular Mycorrhizal ◽

Positive Effects ◽

Nutrient Enhancement ◽

Pinus Greggii ◽

Macro And Micronutrients ◽

First Time

AbstractTraditionally, it is thought that arbuscular mycorrhizae establish a mutualist symbiosis only with the roots of angiosperm plants. In this mutualism, fungi receive carbon from the plants, and angiosperms receive nutrients through the external mycelium of the arbuscular mycorrhizal fungi (AMF). However, the enhanced contents of macro- and micronutrients in gymnosperm plants, and therefore the mutualistic relationship, with AMF has not been reported so far. The present work evaluated whether arbuscular mycorrhizae were able to establish and enhance 9 nutrient contents in the neotropical Pinaceae species Pinus greggii. The tree seedlings were inoculated with three consortia of AMF isolated from an agricultural site, a forest of Cupressus lusitanica and a forest of Pinus hartwegii. The effect of AMF inoculation on plant growth and nutrient enhancement, in addition to colonization, was evaluated. There was evidence of enhancement of plant growth and 9 macro- and micronutrients in plants inoculated with the three evaluated consortia. After 7 months, the translocation was greater for Mg, Mn and Zn in plants inoculated with the consortium of AMF from pine forest. The presence of hyphae, vesicles and arbuscules was detected in the roots of the Pinus greggii plants inoculated with the AMF consortia. In addition to these positive effects, colonization of 10 to 15% and 20 to 38% was observed depending on the AMF consortia after 2 and 7 months, respectively. The presence of arbuscules which is the translocation structure among involved symbionts was also recorded; and photographed for the first time. In the present work, we report for the first time that arbuscular mycorrhiza affects the mobilization of N, P, K, Ca, Mg, Fe, Mn, Zn, Cu and B in gymnosperms, indicating that this mycorrhizal symbiosis is more complex than previously believed.

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