scholarly journals Study of the sensitivity of bacteria of the species Pseudomonas stutzeri and their associates to various inhibitors

2020 ◽  
Vol 27 ◽  
pp. 00118
Author(s):  
T. A. Fedotova ◽  
D. A. Vasiliev ◽  
A. A. Lomakin ◽  
A. A. Nafeev ◽  
V. Y. Lugovtsev
Keyword(s):  
Sodium Azide ◽  
Sodium Benzoate ◽  
Nutrient Medium ◽  
Culture Medium ◽  
Scientific Literature ◽  
Pseudomonas Stutzeri ◽  
Urgent Problem ◽  
Potassium Tellurite ◽  
Environmental Objects ◽  
Pathological Material

Currently, the development of methods for isolation, indication and identification of Pseudomonas stutzeri bacteria from environmental objects and pathological material is an urgent problem. At the same time, there are no data in the scientific literature on the sensitivity of Ps.stutzeri bacteria to various inhibitors, which are necessary for the development of a selective and differential nutrient medium for them. The article presents the results of studying the sensitivity of Ps. stutzeri and their associates to inhibitors such as sodium benzoate, SDS, nalidixic acid, potassium tellurite and sodium azide, which will be used to develop a selective and differential culture medium for Ps.stutzeri bacteria.

The preparation of 3-amino-3-deoxy-1,2-O-isopropylidene-α-L-erythrofuranose, 3-amino-3-deoxy-1,2-O-isopropylidene-β-D-threofuranose and their derivatives

1980 ◽  
Vol 45 (12) ◽  
pp. 3378-3390 ◽  
Author(s):  
Jiří Jarý ◽  
Milena Masojídková ◽  
Ivan Kozák ◽  
Miroslav Marek ◽  
Jan Staněk
Keyword(s):  
Nucleophilic Substitution ◽  
Sodium Azide ◽  
Sodium Benzoate ◽  
Nmr Spectra ◽  
Subsequent Reduction ◽  
H Nmr ◽  
Amino Derivatives

The title amino derivatives VI and XIV were prepared by nucleophilic substitution of p-toluenesulfonyl derivatives II and XVII with sodium azide or hydrazine and subsequent reduction. Nucleophilic substitution of compounds II and XVII with sodium benzoate was also investigated. The 1H NMR spectra of the substances prepared are discussed.

scholarly journals Melanin synthesis by black yeast-like fungi Psedonadsoniella brunnea: dependence of L-tyrosine quantity in the cultural medium

2019 ◽  
Vol 26 (1) ◽  
pp. 41-46 ◽  
Author(s):  
T. Kondratiuk ◽  
T. Beregova ◽  
T. Akulenko ◽  
Ie. Torgalo ◽  
V. Vereschaka
Keyword(s):  
Nutrient Medium ◽  
Culture Medium ◽  
Statistical Processing ◽  
Black Yeast ◽  
Malt Extract ◽  
Melanin Synthesis ◽  
Optimal Conditions ◽  
Cultural Medium ◽  
Yeast Fungi ◽  
Series Of Experiments

To determine the optimal conditions for the synthesis of melanin by black yeast fungi Pseudonadsoniella brunnea (Basidiomycota, Agaricomycotina, Agaricomycetes, Polyporales, Meripilaceae), depending on the amount of L-tyrosine in the culture medium was the purpose of the work. The standard Malt Extract Broth (MEB) liquid nutrient medium was used within this study. L-tyrosine was added to the culture medium in a quantity of 0.01, 0.025 and 0.05%.To obtain the melanin the cultivation of Pseudonadsoniella brunnea was carried out at pH 1-1.5, temperature + 21 ± 1 ° C during 7 days. Statistical processing of the results was carried out using generally accepted methods of variation statistics. It has been established that the level of melanin synthesis by black yeast-like fungi Pseudonadsoniella brunnea depends on the amount of L-tyrosine introduced into the culture medium. The MEB nutrient medium containing 0.05% L-tyrosine in this series of experiments found to be the best composition for obtaining melanin by the strain-producer Pseudonadsoniella brunnea. Compared to control (MEB without L-tyrosine), the amount of melanin synthesized by Ps. brunnea in these conditions increased by 2.5 times. The further research into the optimal conditions for the cultivation of black yeast-like fungi Pseudonadsoniella brunnea in order to obtain melanin is relevant and promising.

scholarly journals Influence of siderophores and iron on Mycobacterium bovis isolation from pathological material

2021 ◽  
Vol 7 (1-2) ◽  
pp. 21-26
Author(s):  
A. I. Zavgorodniy ◽  
S. A. Pozmogova ◽  
V. V. Bilushko ◽  
Kalashnyk Kalashnyk ◽  
O. I. Gologurska
Keyword(s):  
Mycobacterium Bovis ◽  
Culture Filtrate ◽  
Nutrient Medium ◽  
Culture Media ◽  
Alcoholic Extract ◽  
Simultaneous Presence ◽  
Growth Properties ◽  
Pathological Material ◽  
Bacterial Mass

The article presents the results of studying the effect of siderophores and iron on the isolation of Mycobacterium bovis from pathological material. It has been established that the simultaneous presence of iron and siderophore from M. phlei in the nutrient medium makes it possible to detect the growth of M. bovis from pathological material 6–8 days earlier; ensures the growth of more colonies and bacterial mass. The presence of heterologous to mycobacteria siderophore (from Nocardia spp.) in the medium reduces the elective (growth) properties of the medium. Siderophores found in the culture filtrate or alcoholic extract of M. phlei can be valuable additives to culture media for the accelerated isolation of M. bovis from pathological material

scholarly journals A METHOD FOR THE PHYSIOLOGICAL STUDY OF TISSUES IN VITRO

1923 ◽  
Vol 38 (4) ◽  
pp. 407-418 ◽  
Author(s):  
Alexis Carrel
Keyword(s):  
Nutrient Medium ◽  
Bacterial Contamination ◽  
Culture Medium ◽  
Residual Activity ◽  
Physical Factors ◽  
Dynamic Changes ◽  
Fluid Medium ◽  
Continuous Growth ◽  
Pure Cultures

1. A method has been developed which allows the continuous growth of pure strains of fibroblasts, epithelium, and leucocytes in a medium which undergoes but slight spontaneous deterioration. 2. The principle of the method is to leave the tissues undisturbed while the medium is changed. This was realized by special containers allowing the change of the medium without bacterial contamination and by the simultaneous use of a solid and a fluid medium. 3. The curve of growth of pure cultures of fibroblasts and epithelial cells in a nutrient medium is a parabola; in a non-nutrient medium, it is S-shaped and expresses the residual activity of the tissues. Leucocytes invade the culture medium progressively, as do bacteria, but never aggregate in a tissue. 4. The method is used for the study of the morphological and dynamic changes occurring in tissues under the influence of chemical and physical factors.

DIFFERENTIATION STUDIES OF FECAL STREPTOCOCCI FROM FARM ANIMALS

1963 ◽  
Vol 9 (4) ◽  
pp. 443-450 ◽  
Author(s):  
F. M. Ramadan ◽  
M. S. Sabir
Keyword(s):  
Heat Resistance ◽  
Sodium Azide ◽  
Farm Animals ◽  
Fecal Material ◽  
Biochemical Tests ◽  
Potassium Tellurite ◽  
Fecal Streptococcus ◽  
Fecal Streptococci

Employing potassium tellurite, sodium azide, and thallium acetate broths, 361 strains of fecal streptococci were isolated from 142 farm animals representing 11 species. Although none of the three media gave 100% recovery, their collective use made it possible to obtain at least one fecal streptococcus strain from every fecal sample.Results of biochemical tests revealed that 14.8% were S. bovis, 12.7% were S. faecalis var. liquifaciens, and the remaining 72.5% were atypical faecalis groups.Using the 361 isolates from animal fecal material for validating the heat resistance and heat-tellurite tolerance tests for the differentiation between animal and human fecal streptococci strains, it was found that not one of these isolates could survive these combined tests. The importance of these tests is discussed.

scholarly journals The influence of the nutrient medium composition on the induction of calluso- and morphogenesis of Melissa officinalis L.

2020 ◽  
Author(s):  
O.V. Yakimova ◽  
◽  
N.A. Yegorova ◽  
Keyword(s):  
Callus Induction ◽  
Nutrient Medium ◽  
Culture Medium ◽  
Medium Composition ◽  
Ms Medium ◽  
Explant Type ◽  
Maximum Frequency ◽  
Melissa Officinalis ◽  
Exogenous Factors

The features of the calluso- and morphogenesis induction during the cultivation of tissues and organs of Melissa officinalis depending on endogenous and exogenous factors were revealed. The maximum frequency of callus induction (59.5–92.9 %) was noted on the MS medium with 1.0 mg/l 2.4-D and 0.5 mg/l BAP. The induction of morphogenesis from callus was influenced by the composition of the culture medium, the explant type and cultivar. The maximum frequency of morphogenesis induction (20.0–28.0 % depending on the cultivar) from callus was noted on MS culture medium supplemented with 1.0 mg/L NAA and 0.5 mg/L BAP or 1.0 mg/L NAA and 0.5 mg L TDZ.

scholarly journals An innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria

2021 ◽  
Vol 37 ◽  
pp. 00063
Author(s):  
Vladimir Ermakov ◽  
Nikolay Titov
Keyword(s):  
Hydrogen Sulfide ◽  
Nutrient Medium ◽  
Culture Media ◽  
Laboratory Conditions ◽  
Environmental Objects ◽  
Single Section ◽  
Single Compartment ◽  
Petri Dishes ◽  
Ferment Lactose ◽  
Medium Formulation

In laboratory conditions, the modified nutrient differential diagnostic medium Drigalski agar with lactose is recommended to be used for cultivation (isolation) and differentiation of enterobacteria of the Enterobacteriaceae family. The differentiation of enterobacteria on a modified medium is carried out according to their ability to ferment lactose, mannitol, glucose, sucrose, gelatin and form hydrogen sulfide. The environment can also be used for conducting sanitary and microbiological studies of environmental objects. The environment can be used to perform the ONPG test. In the course of preparing the modified Drigalski agar for operation in laboratory conditions, we recommend using the medium according to one of the options: when one of the carbohydrates is added, the medium is poured into single-section reusable or disposable petri dishes; when two carbohydrates are added, the medium is poured into two-section reusable or disposable petri dishes; when a complex of carbohydrates lactose + mannitol and glucose + sucrose is added, the medium is poured into two-section reusable or disposable petri dishes; when using all four drives separately, the medium is dispensed into single-compartment reusable or disposable petri dishes. We recommend preparing modified Drigalski agar at a plant for the production of ready-made culture media with filling the medium in two-section petri dishes (complex of carbohydrates lactose + mannitol in one section, glucose + sucrose in another section), or with filling the medium in four-section petri dishes (with each carbohydrate in separate section).

scholarly journals Pseudomonas Stutzeri in Pathological Material

1968 ◽  
Vol 1 (2) ◽  
pp. 195-202 ◽  
Author(s):  
S. P. LAPAGE ◽  
L. R. HILL ◽  
J. D. REEVE
Keyword(s):  
Pseudomonas Stutzeri ◽  
Pathological Material

scholarly journals STUDIES ON CERTAIN GROWTH PARAMETERS OF DIFFERENT PROBIOTIC STRAINS

2017 ◽  
Vol 23 (4) ◽  
pp. 19-24
Author(s):  
DUMITRA RĂDUCANU ◽  
ANA-MARIA GEORGESCU
Keyword(s):  
Nutrient Medium ◽  
Growth And Development ◽  
Culture Medium ◽  
Growth Parameters ◽  
Probiotic Bacteria ◽  
Solid Culture ◽  
Solid Culture Medium ◽  
Probiotic Strains ◽  
Direct Counting ◽  
Better Than

The researchers found that probiotics contain microorganisms belonging to genus: Streptococcus, Lactobacillus, Bacillus, Aspergillus, Saccharomyces, Enterococcus, Pediococcus, enzymes (lactoperoxidase, gluconase, nonspecific enzymes) and rumen extracts. In this study, commercial probiotic bacteria known as "Linex" were used as samples. Cultural characteristics of these probiotic bacteria have been isolated and studied. It has been found that solid culture medium (nutritional gelose) favored the growth and development of bifidobacteria better than the liquid nutrient medium (nutrient broth). Thus, the number of bifidobacteria resulting from direct counting with Thoma chamber was of 7890 cells.

The Effects of Salinity on the Mosquito Pathogenic Fungus Culicinomyces.

1978 ◽  
Vol 26 (1) ◽  
pp. 55 ◽  
Author(s):  
AW Sweeney
Keyword(s):  
Nutrient Medium ◽  
Culture Medium ◽  
Sea Water ◽  
Pathogenic Fungus ◽  
Hyphal Growth ◽  
Conidial Germination ◽  
Australian Isolate ◽  
Mosquito Larvae

An Australian isolate of the fungus Culicinomyces grows best on nutrient medium without additional salt. Conidial germination and hyphal growth are progressively reduced as the salinity of the culture medium is increased, with very restricted growth at 1.5 times the salinity of sea water. In the laboratory, the fungus will kill mosquito larvae reared at a salinity half that of sea water, but not those reared in sea water, and it appears that the ability of conidia to invade the infection sites of the gut is impaired under the latter conditions.

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