scholarly journals The effectiveness of agrotextile cover with organic photoluminophore in rooting cuttings of Hungarian lilac (Syringa josikaea J. Jacq. ex Rchb.)

2022 ◽  
Vol 42 ◽  
pp. 01017
Author(s):  
Robert Khramov ◽  
Natalia Martynova ◽  
Natalia Besschetnova ◽  
Vladimir Besschetnov ◽  
Yuriy Luponosov
Keyword(s):  
Callus Formation ◽  
Stem Cuttings ◽  
Rooting Of Cuttings ◽  
Vegetation Structures ◽  
Regeneration Processes ◽  
State Agricultural Academy

The effectiveness of the use of a light-transforming shelter of vegetation structures consisting of a polypropylene spunbond with an organic photoluminophore integrated into its structure during the rooting of physiologically active stem cuttings of Hungarian lilac was studied. The object of the study was reproductively mature Hungarian lilac plants located in the arboretum of the Nizhny Novgorod State Agricultural Academy with geographical coordinates 56°14'32.7” N 43°57'20.7”E. The unequal reaction of the tested samples of Hungarian lilac to the use of luminophore in the shelters of vegetation structures during the rooting of cuttings was established, which manifested itself in all characteristics of regeneration processes. High rates of callus formation were in the shelter variants with a higher concentration of luminophore: 73.16 ± 5.95% and 65.25± 4.80%. Lower than in other shelters, the result was recorded in the variant with the lowest luminophore density: 47.00 ± 3.62%.

scholarly journals Effect of different concentrations of indole butyric acid, putrescine and hydrogen peroxide on stem cuttings of the rootstock GF677(Prunus amygdalus × Prunus persica) according to the cutting season

2021 ◽  
Vol 74 (2) ◽  
Author(s):  
Sina Kordzadeh ◽  
Hassan Sarikhani
Keyword(s):  
Hydrogen Peroxide ◽  
Butyric Acid ◽  
Interspecific Hybrid ◽  
Prunus Persica ◽  
Callus Formation ◽  
Stem Cuttings ◽  
Prunus Amygdalus ◽  
Fresh Weight ◽  
Indole Butyric Acid ◽  
Rooting Of Cuttings

The rootstock GF677 is an interspecific hybrid with an important economic and horticultural value. In this research, the effect of indole butyric acid (IBA) in combination with putrescine (Put) and hydrogen peroxide (H2O2) on rooting of GF677 semi-hardwood stem cuttings in three cutting seasons (July, March and October) was investigated. Treatments as IBA (0, 1000, 2000 and 3000 mg L-1), Put (0, 800, 1600 and 3200 mg L-1) and H2O2 (1.5, 3 and 6% w/v) were included. The results showed that in July cuttings, the highest levels of callogenesis were observed in IBA treated cuttings in both concentrations of 1000 and 2000 mg L-1. The rooting was very low in July cuttings, while the highest percentage of rooting (14%) was observed in the combination of 2000 mg L-1 IBA+ 3% H2O2. In March, the cuttings treated by 1000 mg L-1 IBA+800 mg L-1 Put and 1000 mg L-1 IBA+1600 mg L-1 Put revealed the highest percentages of callus formation 83.31 and 83.33%, respectively. In these cuttings, the highest percentage of rooting (63.88%) was gained at 2000 mg L-1 IBA+3200 mg L-1 Put. The application of 1000 mg L-1 IBA+800 mg L-1 Put increased root fresh weight. In cuttings prepared in October, only 800 mg L-1 Put caused callus formation in more than 55% of the cuttings. The rooting of cuttings at this time was as low as the July cuttings, whereas the highest rooting percentage was observed in cuttings treated with IBA at a concentration of 1000 mg L-1. Overall, the experiment showed that the season of the cutting and the treatments with IBA+Put or H2O2 could improve rooting properties of the rootstock GF677.

scholarly journals VEGETATIVE RESCUE AND ROOTING OF CUTTINGS OF DIFFERENT STOCK PLANTS OF Sequoia sempervirens

CERNE ◽  
2017 ◽  
Vol 23 (4) ◽  
pp. 435-444 ◽  
Author(s):  
Mariane de Oliveira Pereira ◽  
Alessandro Camargo Ângelo ◽  
Marcio Carlos Navroski ◽  
Mario Dobner Júnior ◽  
Luciana Magda de Oliveira
Keyword(s):  
Genetic Effect ◽  
Sequoia Sempervirens ◽  
Stem Cuttings ◽  
Fast Growing ◽  
Rooting Of Cuttings ◽  
Living Species ◽  
Recovered Material ◽  
Over 40 Years ◽  
Number Of Shoots

ABSTRACT Sequoia is a fast-growing, long-living species, producing durable timber. The aim of this study was to test different methods for the vegetative rescue of Sequoia sempervirens trees over 40 years old, made at different periods of the year, and, later, testing the rooting of individualized cuttings in planned arrays. Twenty-four individual sequoias were rescued, applying girdling and semi-girdling at three different heights (-10, 0, and 30 cm). The first collection was made 90 days after application of the treatments, being repeated at 150, 240, and 360 days. The percentage of budding trees and the number of shoots per array were registered. In all collections, shoots produced cuttings, which were placed for rooting in mini-tunnels. Cutting survival (%), rooting (%), and number of roots were registered, per array, and per collection. The species vegetative rescue proved to be efficient for the production of shoots for stem cuttings, especially with girdling at 30 cm and semi-girdling at -10 cm. However, it is difficult to define the best method, mainly because of the genetic effect among stock plants. The rooting of cuttings, of recovered material, presented good results (average >65%), also with great differences among stock plants. The potential for rooting of cuttings varied according to different planting periods, with high rooting rates in all seasons, especially in summer. Sequoia sempervirens shows the potential for vegetative rescue and cloning by rooting of cuttings, and this may lead to new studies, with a view towards fixing clones.

scholarly journals   Alanine conjugate of indole-3-butyric acid improves rooting of highbush blueberries

2012 ◽  
Vol 58 (No. 5) ◽  
pp. 236-241 ◽  
Author(s):  
S. Mihaljević ◽  
B. Salopek-Sondi
Keyword(s):  
Butyric Acid ◽  
Vaccinium Corymbosum ◽  
Highbush Blueberry ◽  
Stem Cuttings ◽  
Practical Application ◽  
Rooting Of Cuttings ◽  
Highbush Blueberries ◽  
Set Up ◽  
Indole 3 Acetic Acid

Auxins and their synthetic analogues are commonly used for rooting of cuttings, but their efficiency depends on experimental set-up and, even more importantly, on species or cultivar, and type of explants investigated. In attempt to improve rooting procedure for highbush blueberries (Vaccinium corymbosum L.), we investigated alanine conjugate of indole-3-butyric acid (IBA-Ala) as potential root-promoting compound and compared with commonly used auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The effect of different concentrations of auxins on the rooting of highbush blueberry stem cuttings (var. Bluecrop, Bluetta, Burlington and Jersey) and in vitro-derived microcuttings (var. Jersey) was investigated. Auxin treatments significantly promoted rooting of all four varieties in comparison to control. The most efficient rooting promoter in all varieties appeared IBA-Ala (provided up to 83%, and 93% of the rooted cuttings and microcuttings, respectively). Furthermore, IBA-Ala caused a vigorous, well-branched root system that resulted in better acclimatization and survival of plants. Results suggest the application of IBA-Ala may be beneficial for rooting of difficult-to-root varieties of blueberry and related species and their practical application in the nursery industry.  

scholarly journals Comparison of Rootability of Stem Cuttings from Seedlings of Aesculus sp. and Mature Aesculus × arnoldiana ‘Autumn Splendor’

1988 ◽  
Vol 6 (2) ◽  
pp. 69-71
Author(s):  
B.A. Bergmann ◽  
W.P. Hackett ◽  
H. Pellett
Keyword(s):  
Butyric Acid ◽  
Developmental Stages ◽  
Stem Cuttings ◽  
Mature Tree ◽  
Mature Trees ◽  
Old Growth ◽  
Rooting Of Cuttings ◽  
Root Suckers ◽  
Stem Segments

Rootability was evaluated for stem cuttings taken at varying positions and developmental stages from seedlings of Aesculus and from the mature trees of Aesculus × arnoldiana ‘Autumn Splendor’. Rooting was 100% for cuttings taken from 2-week-old seedlings and 0% for those obtained from the crown of the mature tree 2 months after commencement of spring growth flush. Intermediate rootability was found for cuttings taken from 1-month-old seedlings and from the 2 week-old growth of suckers at the base of the mature tree. Rooting of cuttings with intermediate rooting potential was increased by a quick dip treatment with 2500 ppm indole-3-butyric acid (IBA) or by using apical rather than basal stem segments. Commercially acceptable rooting was not achieved using cuttings from root suckers or the crown of Aesculus ‘Augumn Splendor’.

scholarly journals SHOOT POSITION, IBA AND AGROBACTERIUM RHIZOGENES INFLUENCE PROPAGATION OF HAZELNUT, CORYLUS AVELLANA

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1150g-1150
Author(s):  
William M. Proebsting ◽  
Nahla V. Bassil ◽  
David A. Lightfoot
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Corylus Avellana ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Influence Propagation ◽  
Rooting Of Cuttings ◽  
Shoot Position

Propagation of Corylus avellana stem cuttings may be limited by either root initiation or bud abscission. We divided juvenile shoots of 3 varieties growing in layering beds in mid-July into 4 or 5 3-node cuttings with leaves at the upper two nodes, except that terminal cuttings had one expanded leaf. Cuttings were treated with 5 mM IBA in 50% EtOH, a mixture of A. rhizogenes strains A7 + 22 or left untreated. IBA and bacteria stimulated rooting of cuttings from all shoot positions. Rooting of the terminal cuttings (<50%) was less than that of the sub-terminal cuttings (>80%). Bud retention was <50% on terminal cuttings, nearly 100% on sub-terminal cuttings. Using juvenile stock plants of various varieties, sub-terminal cuttings treated with Agrobacterium or 5 mM IBA may yield 70-90% cuttings with both roots and buds, Agravitropic roots, characteristic of genetic transformation, were observed on Agrobacterium-treated cuttings. Dot blots probed for TL-DNA were negative, however.

scholarly journals Indolebutyric acid on boxwood propagation by stem cuttings

2018 ◽  
Vol 24 (4) ◽  
pp. 347-352 ◽  
Author(s):  
Leandro Marcolino Vieira ◽  
Silvano Kruchelski ◽  
Erik Nunes Gomes ◽  
Katia Christina Zuffellato-Ribas
Keyword(s):  
Statistical Difference ◽  
Callus Formation ◽  
Adventitious Rooting ◽  
Commercial Production ◽  
Stem Cuttings ◽  
Root Number ◽  
Indolebutyric Acid ◽  
Significant Percentage ◽  
Root Numbers ◽  
Buxus Sempervirens

Buxus sempervirens L., popularly known as boxwood, is a woody shrub widely used in gardening and landscaping. One of the most important factors for B. sempervirens commercial production is its propagation. The objective of this study was to evaluate the effect of indolebutyric acid (IBA) concentrations on the adventitious rooting of semihardwood stem cuttings of the species. Branches with new shoots were collected from stock plants with approximately 17 years of growth. Stem cuttings of six centimeters in length with two leaves in the upper region were made with a straight cut at the apex and a bevel cut at the base. Cuttings bases were immersed for 10 seconds in hydroalcoholic solutions (50% v v-1) with IBA at 0, 1500, 3,000 or 6,000 mg L-1. The cuttings were evaluated at 64 and 116 days after planting. Rooting, mortality, callus formation and sprouting percentages as well as root number and root length were measured. At 64 days, no significant percentage of rooting was observed in B. sempervirens stem cuttings and sprouting and callus formation were not significantly affected by the treatments. Keeping the cuttings in the greenhouse for 116 days led to rooting rates up to a 97.5%, with no statistical difference among treatments. Root lengths and root numbers did not differ as a function of treatments. The required period for B. sempervirens stem cuttings rooting is around 116 days; IBA application does not affect rooting, survival or sprouting of B. sempervirens stem cuttings.

Callus Formation and Root Initiation in Stem Cuttings of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco)

1972 ◽  
Vol 2 (2) ◽  
pp. 121-134 ◽  
Author(s):  
J. C. Heaman ◽  
J. N. Owens
Keyword(s):  
Callus Formation ◽  
Douglas Fir ◽  
Vascular Cambium ◽  
Complex Pattern ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Root Primordia ◽  
Weekly Basis ◽  
Vascular Tissues ◽  
Rooting Response

Changes taking place at the base of stem cuttings of Douglas-fir during callus formation and root initiation are described. Material was taken from nine sources, including trees of different ages and with different histories of rooting response. Gross morphology and anatomy of the cutting bases are described using serial longitudinal sections of samples taken on a weekly basis over a 4-month period. The basal callus proliferated from the lowest cells of the vascular cambium and a complex pattern of differentiating vascular tissues arose within the callus mass. The root primordia arose in this callus in association with the differentiating phloem and wound cambium. Variation in callus formation and root initiation is described both within and between clones.

scholarly journals Rooting of Tineke and Natal Briar rose stem cuttings in different substrates

2018 ◽  
Vol 48 (8) ◽  
Author(s):  
Giovana Ritter ◽  
Ellen Toews Doll Hojo ◽  
Fabíola Villa ◽  
Daniel Fernandes da Silva
Keyword(s):  
Experimental Design ◽  
Root Length ◽  
Genetic Material ◽  
Shoot Biomass ◽  
Stem Cuttings ◽  
Coconut Fiber ◽  
Transparent Plastic ◽  
Indole Butyric Acid ◽  
Rooting Of Cuttings ◽  
Two Hybrid

ABSTRACT: The commercial propagation of rose bushes is carried out asexually by the cutting method. The genetic material and the substrate are factors that interfere in the rhizogenic process of the cuttings. Thus, the aim of this work was to evaluate the effect of the substrate on the rooting of cuttings of two hybrid rose bushes. Floriferous branches of matrix plants were cut at the apex and at the base, approximately 8cm long and with two buds, and the basal leaf was removed. The experimental design was completely randomized, in a 2 × 3 factorial scheme (two cultivars, Tineke and Natal Briar × three substrates, carbonized rice husk, coconut fiber and TN Gold®) containing four replicates of 15 cuttings per replicate. The base of the cuttings was immersed for five seconds in IBA 2000mg L-1 solution (indole butyric acid). The cuttings were placed in trays with 24 cells previously filled with substrate and deposited on the floor in a transparent plastic tunnel, built inside a stove. After the 50-day experiment, rootstocks (%), sprouts (%) and dead (%), root length (cm), fresh and dry shoot biomass and roots (g) were evaluated. The rooting percentage of the Natal Briar cultivar was 75%, thus superior to the Tineke cultivar, which had a rooting percentage of 39%. The best substrates for the Tineke and Natal Briar rose cuttings are CAC and coconut fiber, which provided rooting percentages of 81% and 67% of the cuttings, respectively.

scholarly journals Studies on Hormonal Effects on Rooting of Marcotting and Stem-Cuttings of Akee Apple (Blighia sapida K. D. Koenig)

2016 ◽  
Vol 8 (4) ◽  
pp. 468-471 ◽  
Author(s):  
Ehoniyotan Olayemi IBUKUN
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Hormonal Treatment ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Hormonal Treatments

The effect of hormone on the rooting of stem-cuttings and marcotting of akee apple was studied using a combination between Indole -3- Butyric Acid (IBA) and 1- Naphthalene Acetic Acid (NAA). Stem-cuttings from mature akee trees from Challenge, Jalala and Ganmo in Ilorin, were treated with different dilutions of the liquid hormone in the combination of 1.0% Indole-3- butyric acid + 0.5% 1-Naphthaleneacetic acid before propagating them in a non-mist propagator. Marcotting was also carried-out on trees, using the hormonal combination of different dilutions. Observations and the results obtained revealed that the hormonal combination had significant effect on the rooting of stem-cuttings and marcotting. Both marcotting and stem cuttings did not produce at the end of the experiment roots in the absence of the hormonal treatment; a particular aspect was marcotting that initials produced roots. On the other hand, both marcotting and stem cuttings produced roots with the hormonal treatments; more roots were produced using the combination of 2,000 ppm of IBA and 1,000 ppm of NAA, compared with lower concentrations of the hormone mixtures. After callus formation, 2,000 ppm of IBA and 1,000 ppm of NAA combination gave the best results within stem-cuttings. Based on the results obtained, it was concluded that the combination of IBA and NAA in appropriate concentration promoted rooting in Akee apple and therefore are highly valuable for the vegetative propagation of this species through stem cutting and marcotting.

Effects of annual crown pruning and serial propagation on rooting of stem cuttings from Douglas-fir

1983 ◽  
Vol 13 (3) ◽  
pp. 419-424 ◽  
Author(s):  
Donald L. Copes
Keyword(s):  
Douglas Fir ◽  
Clonal Variation ◽  
Stem Cuttings ◽  
Rooting Of Cuttings ◽  
Serial Propagation

Success of rooting from stem cuttings of 14 Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) clones increased significantly from 42% in 1974 to 67% in 1981. The increase was slightly more than 3% each year despite ortet age increasing from 10 and 13 years in 1974 to 17 and 20 years in 1981. The greatest increase occurred from the 3rd through the 6th year of annual rooting trials. Yearly pruning of ortets and rooted ramets of the same clones is thought to have reinvigorated shoots from the pruned crowns of trees. Several annual prunings seemed to be required before average rooting potential increased markedly. No translocation of the reinvigoration stimulus from the pruned to the adjacent unpruned areas of the same trees was detected. Rooting of cuttings from ortets from pruned areas was at least 9% more than cuttings from unpruned areas. No additional invigoration from serial propagation of cuttings was noted for rooting potential, but such cuttings grew a little taller and slightly less plagiotropic 1 year after rooting than were ramets propagated from pruned ortets. Significant clonal variation was found with all treatments. Evidence of a change in physiological vigor of the ortets and rooted ramets was externally visible in the formation of abundant lammas shoots on pruned areas of the 17- and 20-year-old ortets and rooted ramets. Abundant lammas growth is normally found only on Douglas-fir trees less than 10 years old.

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