scholarly journals Effect of different concentrations of indole butyric acid, putrescine and hydrogen peroxide on stem cuttings of the rootstock GF677(Prunus amygdalus × Prunus persica) according to the cutting season

2021 ◽  
Vol 74 (2) ◽  
Author(s):  
Sina Kordzadeh ◽  
Hassan Sarikhani
Keyword(s):  
Hydrogen Peroxide ◽  
Butyric Acid ◽  
Interspecific Hybrid ◽  
Prunus Persica ◽  
Callus Formation ◽  
Stem Cuttings ◽  
Prunus Amygdalus ◽  
Fresh Weight ◽  
Indole Butyric Acid ◽  
Rooting Of Cuttings

The rootstock GF677 is an interspecific hybrid with an important economic and horticultural value. In this research, the effect of indole butyric acid (IBA) in combination with putrescine (Put) and hydrogen peroxide (H2O2) on rooting of GF677 semi-hardwood stem cuttings in three cutting seasons (July, March and October) was investigated. Treatments as IBA (0, 1000, 2000 and 3000 mg L-1), Put (0, 800, 1600 and 3200 mg L-1) and H2O2 (1.5, 3 and 6% w/v) were included. The results showed that in July cuttings, the highest levels of callogenesis were observed in IBA treated cuttings in both concentrations of 1000 and 2000 mg L-1. The rooting was very low in July cuttings, while the highest percentage of rooting (14%) was observed in the combination of 2000 mg L-1 IBA+ 3% H2O2. In March, the cuttings treated by 1000 mg L-1 IBA+800 mg L-1 Put and 1000 mg L-1 IBA+1600 mg L-1 Put revealed the highest percentages of callus formation 83.31 and 83.33%, respectively. In these cuttings, the highest percentage of rooting (63.88%) was gained at 2000 mg L-1 IBA+3200 mg L-1 Put. The application of 1000 mg L-1 IBA+800 mg L-1 Put increased root fresh weight. In cuttings prepared in October, only 800 mg L-1 Put caused callus formation in more than 55% of the cuttings. The rooting of cuttings at this time was as low as the July cuttings, whereas the highest rooting percentage was observed in cuttings treated with IBA at a concentration of 1000 mg L-1. Overall, the experiment showed that the season of the cutting and the treatments with IBA+Put or H2O2 could improve rooting properties of the rootstock GF677.

scholarly journals Maximum efficiency concentration of indole butyric acid in promoting the rooting of Japanese Flowering Cherry

2017 ◽  
Vol 47 (1) ◽  
Author(s):  
Rosimeri de Oliveira Fragoso ◽  
Carlos André Stuepp ◽  
Helena Cristina Rickli ◽  
Katia Christina Zuffellato-Ribas ◽  
Henrique Soares Koehler
Keyword(s):  
Butyric Acid ◽  
Root Formation ◽  
Gradual Increase ◽  
Callus Formation ◽  
Maximum Efficiency ◽  
Stem Cuttings ◽  
Ambient Conditions ◽  
Indole Butyric Acid ◽  
Flowering Cherry ◽  
Low Efficiency

ABSTRACT: Prunus serrulata is a species widely used in the ornamentation and its economic importance is mainly due to the time of its flowering. The objective was to assess the feasibility of cutting technique for propagating P. serrulata , using stem cuttings from current-year shoots, and to estimate the maximum efficiency concentration of auxin indole butyric acid (IBA), in the promotion of rooting. Collection of plant material took place in late spring. Cuttings were made with about 8±1cm length and two leaves reduced to 50% of its original surface. Twenty one IBA concentrations have been assessed (0, 250, 500, 750, 1000, 1250, 1500, 1750, 2000, 2250, 2500, 2750, 3000, 3250, 3500, 3750, 4000, 4250, 4500, 4750 e 5000mg L-1), applied in base of cuttings, which were placed in acclimatized greenhouse. After 60 days, there was a gradual increase in the rooting percentage compared to the increment of IBA to the approximate concentration of 2700mg L-1, with results higher than 80%. In addition, low efficiency concentrations of growth regulators have resulted in fewer roots per cutting, which could compromise the survivability of the seedlings under ambient conditions. Conversely, the decrease in the percentage of callus may be an indication that root formation in P. serrulata occurs directly, without requiring callus formation and its further de-differentiation and re-differentiation in roots.

scholarly journals ROOTING OF GUANANDI (Calophyllum brasilienseCAMBESS) CUTTINGS USING INDOLE-BUTYRIC ACID

CERNE ◽  
2015 ◽  
Vol 21 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Eduardo Ciriello ◽  
Edson Seizo Mori
Keyword(s):  
Butyric Acid ◽  
Vegetative Propagation ◽  
Native Species ◽  
Callus Formation ◽  
Indole Butyric Acid ◽  
Climate Conditions ◽  
Adult Trees ◽  
Logging Industry ◽  
Rooting Of Cuttings ◽  
Improvement Programs

ABSTRACT Commercial reforestation of Brazilian native species to produce hardwood for sawmills has been recently intensified in the country. Among the potential species planted by the logging industry is guanandi (Calophyllum brasiliense Cambess) because it is widely distributed in the country, highly adapted to different soil and climate conditions, good bole form and high quality timber. The development of genetic improvement programs should prioritize gains in productivity and yields in the medium and long term. For such programs to be successful, the study of vegetative propagation techniques to abbreviate steps in forest improvement and allow its mass production is fundamental. To assess the viability of vegetative propagation of the species, two successive experiments were carried out during two years testing the best type of cutting, hormone concentration and management. Different cuttings types submitted to increasing doses of indole-butyric acid (IBA) were tested to evaluate survival, sprouting, rooting and callus formation. Results indicate that the species is viable for vegetative propagation with 85 to 90% rooting of cuttings from seedlings in the IBA concentrations of 3000 to 7000 mg.L-1. For the cuttings, sprouting from the base of adult trees 3000 mg.L-1 was the best concentration of IBA.

scholarly journals Pengaruh Hormon Indole Butyric Acid (IBA) dan 6-Benzyl Amino Purin (BAP) terhadap Induksi Kalus Piper betle L. var Nigra

2019 ◽  
Vol 4 (2) ◽  
pp. 85-90
Author(s):  
Junairiah . ◽  
Artifa Rachmah ◽  
Yosephine Sri Wulan Manuhara ◽  
Ni’matuzahroh . ◽  
Lilis Sulistyorini ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Callus Formation ◽  
Dry Weight ◽  
Piper Betle ◽  
Fresh Weight ◽  
Indole Butyric Acid ◽  
Randomized Design ◽  
Benzyl Amino Purine

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh kombinasi konsentrasi zat pengatur tumbuh IBA (Indole Butyric Acid) dan BAP (6-Benzyl Amino Purine) yang paling baik untuk induksi kalus sirih hitam (Piper betle L.). Penelitian ini menggunakan rancangan acak lengkap dengan 25 perlakuan dan setiap perlakuan memiliki 6 ulangan sehingga terdapat 150 unit ekperimen. Pada tahap kultur kalus dilakukan dengan menambahkan zat pengatur tumbuh IBA dan BAP ke dalam medium Murashige and Skoog (MS). Hasil uji tersebut menunjukkan bahwa zat pengatur tumbuh IBA dan BAP dengan kombinasi konsentrasi berbeda berpengaruh terhadap waktu induksi kalus, berat segar dan berat kering kalus sirih hitam. Hasil penelitian menunjukkan waktu tercepat pembentukan kalus pada IBA 2,0 mg/L dan BAP 2,0 mg/L yaitu 10 hari. Berat segar dan berat kering tertinggi pada IBA 2,0 mg/L dan 2,0 mg/L yaitu 0,8507 gram untuk berat segar dan 0,0769 untuk berat kering. Warna kalus adalah putih kehijauan dengan tekstur kompak dan remah.Kata kunci: Induksi kalus, Piper betle L., Indole Butyric Acid, 6-Benzyl Amino Purine.ABSTRACTThe purpose of this research to determine the effect of the combination concentration of growth regulators IBA (Indole Butyric Acid) and BAP (6-Benzyl Amino Purine) was best for callus induction black betel (Piper betle L.). This research used completely randomized design with 25 treatments and 6 replicates of each treatment, hence there were 150 experimental units. At this stage of callus culture was done by adding the growth regulators IBA and BAP into Murashige and Skoog (MS). The test results showed that plant growth regulators IBA and BAP in combination with different concentrations of influence on callus induction time, fresh weight and dry weight callus Piper betle L. The results showed the fastest time of callus formation at IBA 2,0 mg/L and BAP 2,0 mg/L at 10 days. Fresh weight and dry weight of the highest in the IBA 2,0 mg/L and BAP 2,0 mg/L were 0,8507 grams and 0,0769 grams fresh weight to dry weight. The color of callus was white greenish with compact and friable texture.Keywords: Callus induction, Piper betle L., Indole Butyric Acid, 6-Benzyl Amino Purine.

scholarly journals Propagation of three native Brazilian Piper species by cuttings and indole butyric acid

2018 ◽  
Vol 12 (2) ◽  
pp. 491-499
Author(s):  
Aurea Portes Ferriani ◽  
Cícero Deschamps ◽  
Wanderlei Do Amaral ◽  
Luiz Everson da Silva
Keyword(s):  
New Species ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Biological Activities ◽  
Average Diameter ◽  
Adventitious Rooting ◽  
Stem Cuttings ◽  
Indole Butyric Acid ◽  
Positive Effect ◽  
Biological Reserve

Native Piper species present significant repellent, antimicrobial, inseticidal, anti-tumor and anti-protozoal biological activities. Studies on new species can discover unpublished potentialities and vegetative propagation for the development of cultivation protocols and reduce the natural extraction. The objective of this study was to evaluate the rooting of P. arboreum, P. cernuum and P. diospyrifolium stem cuttings with different treatments containing doses of indole butyric acid (0, 500; 1,000; 1,500; 2,000 and 3,000 mg L-1). Branches were collected in the “Bom Jesus Biological Reserve”, Parana state, Brazil. The cuttings, with a length of 12 cm and average diameter of 6 mm, were placed in 53 cm3 plastic tubes with the commercial substrate Tropstrato HP® and intermittent misting. After 90 days, the survival, mortality, cuttings with new shoots, number and length of the three main roots were evaluated. The percentages of rooting reached an average of 67.5% for P. arboreum, 51.6% for P. cernuum and 50.4% for P. diospyrifolium. A positive effect in the treatments containing the plant regulator was observed for rooting development, percentage of cuttings with shoots and number of roots per propagule, but there were variations in the responses of each species. Leaf retention was an important factor for the adventitious rooting formation in all evaluated species.

scholarly journals   Alanine conjugate of indole-3-butyric acid improves rooting of highbush blueberries

2012 ◽  
Vol 58 (No. 5) ◽  
pp. 236-241 ◽  
Author(s):  
S. Mihaljević ◽  
B. Salopek-Sondi
Keyword(s):  
Butyric Acid ◽  
Vaccinium Corymbosum ◽  
Highbush Blueberry ◽  
Stem Cuttings ◽  
Practical Application ◽  
Rooting Of Cuttings ◽  
Highbush Blueberries ◽  
Set Up ◽  
Indole 3 Acetic Acid

Auxins and their synthetic analogues are commonly used for rooting of cuttings, but their efficiency depends on experimental set-up and, even more importantly, on species or cultivar, and type of explants investigated. In attempt to improve rooting procedure for highbush blueberries (Vaccinium corymbosum L.), we investigated alanine conjugate of indole-3-butyric acid (IBA-Ala) as potential root-promoting compound and compared with commonly used auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The effect of different concentrations of auxins on the rooting of highbush blueberry stem cuttings (var. Bluecrop, Bluetta, Burlington and Jersey) and in vitro-derived microcuttings (var. Jersey) was investigated. Auxin treatments significantly promoted rooting of all four varieties in comparison to control. The most efficient rooting promoter in all varieties appeared IBA-Ala (provided up to 83%, and 93% of the rooted cuttings and microcuttings, respectively). Furthermore, IBA-Ala caused a vigorous, well-branched root system that resulted in better acclimatization and survival of plants. Results suggest the application of IBA-Ala may be beneficial for rooting of difficult-to-root varieties of blueberry and related species and their practical application in the nursery industry.  

scholarly journals Comparison of Rootability of Stem Cuttings from Seedlings of Aesculus sp. and Mature Aesculus × arnoldiana ‘Autumn Splendor’

1988 ◽  
Vol 6 (2) ◽  
pp. 69-71
Author(s):  
B.A. Bergmann ◽  
W.P. Hackett ◽  
H. Pellett
Keyword(s):  
Butyric Acid ◽  
Developmental Stages ◽  
Stem Cuttings ◽  
Mature Tree ◽  
Mature Trees ◽  
Old Growth ◽  
Rooting Of Cuttings ◽  
Root Suckers ◽  
Stem Segments

Rootability was evaluated for stem cuttings taken at varying positions and developmental stages from seedlings of Aesculus and from the mature trees of Aesculus × arnoldiana ‘Autumn Splendor’. Rooting was 100% for cuttings taken from 2-week-old seedlings and 0% for those obtained from the crown of the mature tree 2 months after commencement of spring growth flush. Intermediate rootability was found for cuttings taken from 1-month-old seedlings and from the 2 week-old growth of suckers at the base of the mature tree. Rooting of cuttings with intermediate rooting potential was increased by a quick dip treatment with 2500 ppm indole-3-butyric acid (IBA) or by using apical rather than basal stem segments. Commercially acceptable rooting was not achieved using cuttings from root suckers or the crown of Aesculus ‘Augumn Splendor’.

scholarly journals Rooting of Tineke and Natal Briar rose stem cuttings in different substrates

2018 ◽  
Vol 48 (8) ◽  
Author(s):  
Giovana Ritter ◽  
Ellen Toews Doll Hojo ◽  
Fabíola Villa ◽  
Daniel Fernandes da Silva
Keyword(s):  
Experimental Design ◽  
Root Length ◽  
Genetic Material ◽  
Shoot Biomass ◽  
Stem Cuttings ◽  
Coconut Fiber ◽  
Transparent Plastic ◽  
Indole Butyric Acid ◽  
Rooting Of Cuttings ◽  
Two Hybrid

ABSTRACT: The commercial propagation of rose bushes is carried out asexually by the cutting method. The genetic material and the substrate are factors that interfere in the rhizogenic process of the cuttings. Thus, the aim of this work was to evaluate the effect of the substrate on the rooting of cuttings of two hybrid rose bushes. Floriferous branches of matrix plants were cut at the apex and at the base, approximately 8cm long and with two buds, and the basal leaf was removed. The experimental design was completely randomized, in a 2 × 3 factorial scheme (two cultivars, Tineke and Natal Briar × three substrates, carbonized rice husk, coconut fiber and TN Gold®) containing four replicates of 15 cuttings per replicate. The base of the cuttings was immersed for five seconds in IBA 2000mg L-1 solution (indole butyric acid). The cuttings were placed in trays with 24 cells previously filled with substrate and deposited on the floor in a transparent plastic tunnel, built inside a stove. After the 50-day experiment, rootstocks (%), sprouts (%) and dead (%), root length (cm), fresh and dry shoot biomass and roots (g) were evaluated. The rooting percentage of the Natal Briar cultivar was 75%, thus superior to the Tineke cultivar, which had a rooting percentage of 39%. The best substrates for the Tineke and Natal Briar rose cuttings are CAC and coconut fiber, which provided rooting percentages of 81% and 67% of the cuttings, respectively.

scholarly journals Studies on Hormonal Effects on Rooting of Marcotting and Stem-Cuttings of Akee Apple (Blighia sapida K. D. Koenig)

2016 ◽  
Vol 8 (4) ◽  
pp. 468-471 ◽  
Author(s):  
Ehoniyotan Olayemi IBUKUN
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Hormonal Treatment ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Hormonal Treatments

The effect of hormone on the rooting of stem-cuttings and marcotting of akee apple was studied using a combination between Indole -3- Butyric Acid (IBA) and 1- Naphthalene Acetic Acid (NAA). Stem-cuttings from mature akee trees from Challenge, Jalala and Ganmo in Ilorin, were treated with different dilutions of the liquid hormone in the combination of 1.0% Indole-3- butyric acid + 0.5% 1-Naphthaleneacetic acid before propagating them in a non-mist propagator. Marcotting was also carried-out on trees, using the hormonal combination of different dilutions. Observations and the results obtained revealed that the hormonal combination had significant effect on the rooting of stem-cuttings and marcotting. Both marcotting and stem cuttings did not produce at the end of the experiment roots in the absence of the hormonal treatment; a particular aspect was marcotting that initials produced roots. On the other hand, both marcotting and stem cuttings produced roots with the hormonal treatments; more roots were produced using the combination of 2,000 ppm of IBA and 1,000 ppm of NAA, compared with lower concentrations of the hormone mixtures. After callus formation, 2,000 ppm of IBA and 1,000 ppm of NAA combination gave the best results within stem-cuttings. Based on the results obtained, it was concluded that the combination of IBA and NAA in appropriate concentration promoted rooting in Akee apple and therefore are highly valuable for the vegetative propagation of this species through stem cutting and marcotting.

Studies on the regeneration of peach cultivars and rootstocks from root cuttings in comparison with aerial cuttings

2006 ◽  
Vol 46 (8) ◽  
pp. 1091
Author(s):  
C. G. Tsipouridis ◽  
W. W. Schwabe
Keyword(s):  
Root Formation ◽  
Prunus Persica ◽  
Inverse Correlation ◽  
Prunus Domestica ◽  
Prunus Amygdalus ◽  
Benzyl Adenine ◽  
Indole Butyric Acid ◽  
Significant Difference ◽  
Bottom Heat ◽  
Peach Trees

This study showed that shoot cuttings collected from peach trees in February and November gave the highest sprouting percentages. A comparison of 3 cultivars of Prunus persica L. Batch (ArmKing, Early Crest and May Crest) showed that November sampling was optimal for all cultivars. Although the sprouting of root cuttings gave much lower percentages, the peak values occurred at exactly the same times of year as those for root formation. The results also showed that the effectiveness of indole-butyric acid treatment to induce root formation on detached root-shoots was greater when the seasonal rooting level is low. However, this inverse correlation is not significant when all 12 monthly values are combined. The data for comparing the 2 rootstocks and their interaction with the concentration of benzyl-adenine-purine applied showed that St Julien GF655/2 (Prunus insititia) had more productive root-shoots and better growth than Damas GF1869 (Prunus domestica × Prunus spinosa). There was no significant difference between different concentrations of benzyl-adenine-purine, but benzyl-adenine-purine affected the morphology of the young root-shoots. Cold storage for 1 month had a beneficial effect on sprouting of root cuttings of the rootstock GF677 (Prunus persica × Prunus amygdalus) and treatments with bottom heat increased sprouting over that achieved in the growth room. X-ray treated roots produced significantly fewer sprouts.

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