scholarly journals Characteristics of the Genetic Variation of a Swamp Buffalo (Bubalusbubalis) of South Sumatra Based on Polymerase Chain Reaction-Random Amplified Polymorphic DNA (PCR-RAPD)

2018 ◽  
Vol 68 ◽  
pp. 01002
Author(s):  
Yuanita Windusari ◽  
Laila Hanum ◽  
Arum Setiawan ◽  
Veronika Larasati
Keyword(s):  
Genetic Variation ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Approach ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Polymerase Chain ◽  
Polymorphic Bands ◽  
Swamp Buffalo ◽  
Dna Pcr ◽  
Cluster 2

Swamp buffalo (Bubalusbubalis) is one of the endemic species that become a wealth of genetic resources of South Sumatra. This study aims to the genetic variation and relationships of kinship 6 variants of swamp buffalo South Sumatera. The methods used by the molecular approach using RAPD-PCR primer 5 i.e. ILO 1204, ILO 1212, ILO 525, OPW 03 and OPY 13. Data was analyzed using SPSS ver 16.0 and presented in dendrogram. The results of the amplification, all primary produce band with a total of 63 band of DNA (14.92%) with an average of every primary produce 12.6 band of DNA. The most primary produce DNA polymorphic bands namely OPW 03 (23.81%) and ILO 1204 (20.63%), while the primary ILO 525 (0.00%) do not generate polymorphic bands. Genetic variation of swamp buffalo has a low genetic variation with 14.92% percentage it generated polymorphic bands. The results of the dendogram obtained two clusters namely cluster 1 included Kerbau Tanduk Bulat, Kerbau Tanduk Langit, Kerbau Tanduk Melintang and Kerbau Tanduk Dungkul, while the cluster 2 of them Kerbau Bule and Kerbau Rebah Belakang. Swamp buffalo variants that have the closest genetic distance. Kerbau Tanduk Langit and Kerbau Tanduk Bulat with 856 coefficient similarity, while the farthest Kerbau Tanduk Langit and Kerbau Bule with the coefficient similarity -972. Swamp buffalo (Bubalusbubalis) of South Sumatera, which consists of 6 variants of buffalo have low genetic variation and inbreeding of closekinship.

Classification of Australian garlic cultivars by DNA fingerprinting

1996 ◽  
Vol 36 (5) ◽  
pp. 613 ◽  
Author(s):  
KF Bradley ◽  
MA Rieger ◽  
GG Collins
Keyword(s):  
Allium Sativum ◽  
Random Amplified Polymorphic Dna ◽  
Growing Season ◽  
Chain Reaction ◽  
Binary Format ◽  
Rapd Pcr ◽  
Polymerase Chain ◽  
Polymorphic Bands ◽  
Descriptive Names

Garlic (Allium sativum L.) reproduces only by vegetative propagation yet displays considerable morphological variation within and between cultivars. The origins of Australian cultivars are uncertain and the descriptive names applied to them may not reflect their derivation. Twenty common Australian garlic cultivars were analysed by the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique using 20 random decamer primers. The amplification products of 5 of these primers resulted in 65 clear polymorphic bands. These bands were transformed into a binary format, and genetic similarities calculated using a simple matching coefficient. The similarities were used to perform a cluster analysis and produce a dendrogram grouping the cultivars. Bolting and intermediate/non-bolting types could be differentiated from each other. These could be further subdivided into 4 groups based on length of growing season.

scholarly journals KERAGAMAN GENETIK, KEBUGARAN DAN INKOMPATIBILITAS REPRODUKSI Hemiptarsenus varicornis Girault (Hymenoptera: Eulophidae), PARASITOID LARVA Liriomyza huidobrensis (Diptera: Agromyzidae)

2011 ◽  
Vol 11 (1) ◽  
pp. 1-10
Author(s):  
Reflinaldon Reflinaldon ◽  
Damayanti Buchori ◽  
Dwinardi Aprianto
Keyword(s):  
Genetic Variation ◽  
Dna Polymerase ◽  
Random Amplified Polymorphic Dna ◽  
Similarity Coefficient ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Reproductive Incompatibility ◽  
Liriomyza Huidobrensis ◽  
Geographic Populations ◽  
Polymerase Chain

Several experiments have been conducted to study genetic variation, fitness and reproductive incompatibility of H. varicornis from different geographic populations.  Genetic variation from Pandai Sikek (PS), Alahan Panjang (AP) and Kayu Aro (KA) was analyzed by using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique and the similarity of genetics measured using NTSys program. The fitness of female wasps such as longevity, fecundity and preoviposition was observed and then compared among those populations.  Incompatibility in reproduction was determined by accounting of reproductive compatibility (RC) index in crossing of intra and interpopulation both of PS and AP.  The results showed high genetic variation of H. varicornis among population from Alahan Panjang, Pandai Sikek and Kayu Aro with similarity coefficient of 30 to 70%.  The best fitness showed the female wasps from Kayu Aro that was significantly different (P= 0.00) in longevity (24.60 ± 6.4 days), fecundity (63.6 ± 28.6 eggs) and parasitization (53.60%) but not significantly different (P=0.07) in number of the first day eggs (1.1 ± 1.4 eggs). Crossing of AP and PS indicated incompatibility in reproduction among the population.

Use of the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) to detect DNA polymorphisms in aphids (Homoptera: Aphididae)

1992 ◽  
Vol 82 (2) ◽  
pp. 151-159 ◽  
Author(s):  
William C. Black ◽  
Nancy M. DuTeau ◽  
Gary J. Puterka ◽  
James R. Nechols ◽  
Jennifer M. Pettorini
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Variation ◽  
Acyrthosiphon Pisum ◽  
Random Amplified Polymorphic Dna ◽  
Diaeretiella Rapae ◽  
New Technique ◽  
Species Variation ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Polymerase Chain

AbstractWe have used a new technique to identify discrete genetic markers in aphids, a family in which biochemical and morphological genetic polymorphisms are rare. The new technique uses the polymerase chain reaction (PCR) to amplify random regions of aphid genomes (random amplified polymorphic DNA) and has been termed RAPD-PCR. We demonstrate the use of the technique in revealing genetic variation in four aphid species, the greenbug (Schizaphis graminum (Rondani)), the Russian wheat aphid (Diuraphis noxia (Mordvilko)), the pea aphid (Acyrthosiphon pisum (Harris)), and the brown ambrosia aphid (Uroleucon ambrosiae (Thomas)). In contrast with allozyme surveys, RAPD-PCR revealed large amounts of genetic variation among individuals in each of these species. Variation was detected among biotypes, populations, colour morphs and even individuals on a single plant. We also explored the utility of RAPD-PCR in the detection and identification within aphid bodies of two endoparasitic wasps, Diaeretiella rapae (McIntosh) and Lysiphlebus testaceipes (Cresson). The use of RAPD-PCR in species diagnostics, parasitoid detection, and population studies is discussed.

scholarly journals Genetic diversity analysis of endemic species, Garcinia cambogia Gaertn using randomly amplified Polymorphic DNA markers

2017 ◽  
Vol 7 ◽  
Author(s):  
Santosh P ◽  
Suresh B. Arakera
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Consumer Preference ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Garcinia Cambogia ◽  
Polymerase Chain ◽  
Polymorphic Bands ◽  
Dna Pcr

<p><strong>Polymerase chain reaction based random amplified polymorphic DNA (PCR-RAPD) markers were employed to assess genetic diversity in eight <em>Garcinia  cambogia</em>  genotypes. Among the 20 random primers used in the present investigation, 9 primers showed polymorphism. A total number of 227 bands were obtained from 9 primers, out of which 225 were polymorphic, showing 99.11% polymorphism. An average of 25.22 bands per primer was scored and average number of polymorphic bands found to be 25. The eight accessions fall into two major clusters. Cluster analysis showed that the red and yellow accessions cannot be regarded as two different varieties. The use of red and yellow fruits for commercial and medicinal purposes, respectively, is purely based on consumer preference. </strong></p>

Sign in / Sign up

Export Citation Format

Share Document