Classification of Australian garlic cultivars by DNA fingerprinting

1996 ◽  
Vol 36 (5) ◽  
pp. 613 ◽  
Author(s):  
KF Bradley ◽  
MA Rieger ◽  
GG Collins
Keyword(s):  
Allium Sativum ◽  
Random Amplified Polymorphic Dna ◽  
Growing Season ◽  
Chain Reaction ◽  
Binary Format ◽  
Rapd Pcr ◽  
Polymerase Chain ◽  
Polymorphic Bands ◽  
Descriptive Names

Garlic (Allium sativum L.) reproduces only by vegetative propagation yet displays considerable morphological variation within and between cultivars. The origins of Australian cultivars are uncertain and the descriptive names applied to them may not reflect their derivation. Twenty common Australian garlic cultivars were analysed by the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique using 20 random decamer primers. The amplification products of 5 of these primers resulted in 65 clear polymorphic bands. These bands were transformed into a binary format, and genetic similarities calculated using a simple matching coefficient. The similarities were used to perform a cluster analysis and produce a dendrogram grouping the cultivars. Bolting and intermediate/non-bolting types could be differentiated from each other. These could be further subdivided into 4 groups based on length of growing season.

scholarly journals Characteristics of the Genetic Variation of a Swamp Buffalo (Bubalusbubalis) of South Sumatra Based on Polymerase Chain Reaction-Random Amplified Polymorphic DNA (PCR-RAPD)

2018 ◽  
Vol 68 ◽  
pp. 01002
Author(s):  
Yuanita Windusari ◽  
Laila Hanum ◽  
Arum Setiawan ◽  
Veronika Larasati
Keyword(s):  
Genetic Variation ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Approach ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Polymerase Chain ◽  
Polymorphic Bands ◽  
Swamp Buffalo ◽  
Dna Pcr ◽  
Cluster 2

Swamp buffalo (Bubalusbubalis) is one of the endemic species that become a wealth of genetic resources of South Sumatra. This study aims to the genetic variation and relationships of kinship 6 variants of swamp buffalo South Sumatera. The methods used by the molecular approach using RAPD-PCR primer 5 i.e. ILO 1204, ILO 1212, ILO 525, OPW 03 and OPY 13. Data was analyzed using SPSS ver 16.0 and presented in dendrogram. The results of the amplification, all primary produce band with a total of 63 band of DNA (14.92%) with an average of every primary produce 12.6 band of DNA. The most primary produce DNA polymorphic bands namely OPW 03 (23.81%) and ILO 1204 (20.63%), while the primary ILO 525 (0.00%) do not generate polymorphic bands. Genetic variation of swamp buffalo has a low genetic variation with 14.92% percentage it generated polymorphic bands. The results of the dendogram obtained two clusters namely cluster 1 included Kerbau Tanduk Bulat, Kerbau Tanduk Langit, Kerbau Tanduk Melintang and Kerbau Tanduk Dungkul, while the cluster 2 of them Kerbau Bule and Kerbau Rebah Belakang. Swamp buffalo variants that have the closest genetic distance. Kerbau Tanduk Langit and Kerbau Tanduk Bulat with 856 coefficient similarity, while the farthest Kerbau Tanduk Langit and Kerbau Bule with the coefficient similarity -972. Swamp buffalo (Bubalusbubalis) of South Sumatera, which consists of 6 variants of buffalo have low genetic variation and inbreeding of closekinship.

DNA FINGERPRINTING OF SINGLE APHID EMBRYOS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD–PCR)

1999 ◽  
Vol 131 (2) ◽  
pp. 229-230 ◽  
Author(s):  
C.K. Chan ◽  
D.J. Petersen ◽  
T.C. Vrain
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Acyrthosiphon Pisum ◽  
Random Amplified Polymorphic Dna ◽  
Aphis Gossypii ◽  
Aphis Fabae ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Laboratory Colonies ◽  
Polymerase Chain

Extraction of DNA from whole aphids, in combination with random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) (Williams et al. 1990) markers can detect interspecific and intraspecific genetic variation (Black et al. 1992; Cenis et al. 1993). However, these techniques entail destructive sampling of fresh or preserved specimens. To allow experimental replication from a single sample while preserving the same aphid for morphometrical or karyotyping analyses, we describe a technique for RAPD-PCR using DNA from single aphid embryos. We evaluated the usefulness and reliability of single-embryo analysis, using four species of our laboratory colonies, namely Acyrthosiphon pisum (Harris), Aphis fabae Scopoli, Aphis frangulae group, and Aphis gossypii Glover.

scholarly journals Mycoplasma gallisepticum strains with identical random amplified polymorphic DNA (RAPD) patterns in chukar partridges (Alectoris chukar) and broilers: a case report

2013 ◽  
Vol 58 (No. 5) ◽  
pp. 284-288
Author(s):  
R. Khoshbakht ◽  
S. Seifi ◽  
M. Tabatabaei ◽  
H. Shirzad Aski ◽  
V. Ranjbar ◽  
...  
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Mycoplasma Gallisepticum ◽  
Common Source ◽  
Chain Reaction ◽  
Control Programs ◽  
Alectoris Chukar ◽  
Rapd Pcr ◽  
Polymerase Chain ◽  
Chukar Partridge ◽  
Significant Disease

We used the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique to discriminate the major emerging poultry pathogen, Mycoplasma gallisepticum (MG), in broiler and chukar partridge cases referred to the veterinary medicine teaching hospital. Amazingly, the chickens and partridges random amplified polymorphic DNA (RAPD) patterns were similar. This suggests the risk of a common source for the strains isolated from the different animals and illustrates the necessity of novel and improved control programs to prevent and restrict this significant disease which is prevalent among poultry species.  

scholarly journals Optimization of RAPD-PCR conditions for the study of genetic diversity in Nepal’s Swertia chirayita (Roxb. Ex Fleming) H. Karst

2011 ◽  
Vol 6 (8) ◽  
pp. 35-40
Author(s):  
Sangita Shrestha ◽  
Jaishree Sijapati ◽  
Neesha Rana ◽  
Diwa Malla ◽  
Prabha Regmi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Polymerase Chain Reaction ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Molecular Diagnostic ◽  
Chain Reaction ◽  
Natural Habitats ◽  
Molecular Genetic Diversity ◽  
Rapd Pcr ◽  
Polymerase Chain

Of the 30 species (including five varieties) of the genus Swertia in Nepal, nine have been reported to possess medicinal properties. Among these, S. chirayita is the most valuable species, with high demand in domestic and international markets. Nepal’s S. chirayita and related species are being recklessly exploited for commercial purposes. Two problems that have emerged with this lucrative market are (a) adulteration and fraudulent labeling of S. chirayita, and (b) depletion of S. chirayita and allied species from their natural habitats. To address the problem of adulteration and conservation, we studied molecular genetic diversity in S. chirayita populations and developed a molecular diagnostic tool for the purposes of authentication. We studied intra-specific genetic diversity in S. chirayita using Polymerase Chain Reaction (PCR)-based Random Amplified Polymorphic DNA (RAPD) technique. As a preliminary step, we identified optimal RAPD-PCR reaction and cycling conditions by varying PCR reaction parameters such as concentration of template DNA, MgCl2, dNTPs, primer, Taq DNA polymerase and RAPD-PCR programs. The optimized PCR reaction and cycling conditions were then used in subsequent RAPD profiling experiments for the study of genetic diversity within S. chirayita populations from various geographical locations. Genetic diversity characterization of S. chirayita populations at the molecular level would furnish information with significant applications in the conservation and sustainable utilization of S. chirayita and its allied species in Nepal. Key words: Polymerase Chain Reaction, Random Amplified Polymorphic DNA, DNA fingerprinting, genetic diversity DOI: http://dx.doi.org/10.3126/hjs.v6i8.2699 Himalayan Journal of Sciences Vol.6 Issue 8 2010 pp.35-40

Sign in / Sign up

Export Citation Format

Share Document