scholarly journals Bioethanol Production from Microalgae Oscillatoria sp Cultured in Blue Green 11 and Bold Basal Media

2018 ◽  
Vol 68 ◽  
pp. 03018
Author(s):  
Hermansyah ◽  
Rona Ayu Sakinah ◽  
Julinar ◽  
Zazili Hanafiah ◽  
Hilda Zulkifli
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Hplc Analysis ◽  
Fermentation Process ◽  
Basal Medium ◽  
Third Generation ◽  
Bioethanol Production ◽  
The Third ◽  
Microalgae Biomass ◽  
Basal Media ◽  
Microbial Agent

The third generation of bioethanol production, bioethanol converted from biomass of microalgae. Our reserach aimed to produce bioethanol from substrate microalgae Oscillatoria sp. as substrate has been conducted. Oscillatoria sp. cultured in two media namely Bold Basal and Blue Green 11. microalgae Oscillatoria sp. were cultivated in Bold Basal and Blue Green 11 media hydrolyzed substrate, 0.143 mL/g and 0.496 mL/g, respectively. Unhyrolysate microalgae generated 0.018 mL/g and 3.59 mL/g of reduced sugar, respectively. Biomass obtained from Blue Green 11 media and Bold Basal media were 0.676 g and 0.482 g, respectively. Based on HPLC analysis, Oscillatoria sp. biomass contained maltose. Biomass were hydrolyzed by autoclaving in 121°C for 15 minutes. Bioethanol production was conducted by fermentation process using Saccharomyces cerevisiae as a microbial agent which applied in hydrolysate and non-hydrolysate of microalgae. Ethanol generated from fermentation was analyzed using gas chromatography. Based on gass chromatography data resulted that hydrolysate of microalgae biomass cultivated in Bold Basal medium and in Blue Green 11 medium produced 7% and 23% ethanol, respectively.

Advances of macroalgae biomass for the third generation of bioethanol production

2020 ◽  
Vol 28 (2) ◽  
pp. 502-517 ◽  
Author(s):  
Inn Shi Tan ◽  
Man Kee Lam ◽  
Henry Chee Yew Foo ◽  
Steven Lim ◽  
Keat Teong Lee
Keyword(s):  
Third Generation ◽  
Bioethanol Production ◽  
The Third

scholarly journals PENGARUH KADAR Aspergillus niger TERHADAP PRODUKSI BIOETANOL DARI BONGGOL PISANG KEPOK ( Musa paradisiaca L)

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Junaini Junaini ◽  
Elvinawati Elvinawati ◽  
Sumpono Sumpono
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Specific Gravity ◽  
Fermentation Process ◽  
Bioethanol Production ◽  
Gravity Method ◽  
Musa Paradisiaca ◽  
Ethanol Content

This study aims to determine the effect of Aspergillus niger levels on bioethanol production in banana cobs using Saccharfication Simultation Fermentation (SSF) method. This research uses banana kepok (Musa paradisiaca L.) obtained from Enggano Island of Bengkulu Province. Enggano Island is one of the outermost islands of Bengkulu Province which has a coordinate point of 5023'25,000 '' LS - 102014'16,000 '' BT. Samples of banana done preparation before the hydrolysis and fermentation process by smoothing the banana cobs using a blender until it becomes mush. Samples in the form of slurry were then added by Aspergillus niger and Sccharomyces cerevisiae. Hydrolysis performed for 72 hours which then continued with the fermentation process for 5 days. In the study there were 5 treatments: addition of Aspergillus niger 107 CFU/mL, addition of 10 mL Saccharomyces cerevisiae, addition of 10 mL Saccharomyces cerevisiae + Aspergillus niger 106 CFU/mL, 10 mL Saccharomyces cerevisiae + Aspergillus niger 107CFU/mL and 10 mL Saccharomyces cerevisiae + Aspergillus niger 108CFU/mL. The fermentation results were distilled and then measured the ethanol content by the specific gravity method. Ethanol content obtained from each treatment were 3.995%, 6.218%, 6.825%, 9.065%, and 12.348%, respectively. From one-way analysis test can be obtained the value of Fcount and Ftabel respectively are 25.73 and 5.19, so the value of Ftable< Fcount which means each treatment has a different result significantly.

scholarly journals Selenium supplementation during fermentation with sugar beet molasses and Saccharomyces cerevisiae to increase bioethanol production

2019 ◽  
Vol 8 (1) ◽  
pp. 622-628 ◽  
Author(s):  
Sara Faramarzi ◽  
Younes Anzabi ◽  
Hoda Jafarizadeh-Malmiri
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sugar Beet ◽  
Sodium Selenite ◽  
Fermentation Process ◽  
Selenium Supplementation ◽  
Bioethanol Production ◽  
Beet Molasses ◽  
Sugar Beet Molasses ◽  
Fermentation Parameters ◽  
Microbial Strain

Abstract A bench scale submerged fermentation process was used to bioethanol produce using sugar beet molasses and Saccharomyces cerevisiae, as substrate and microbial strain, respectively. Effects of selenium amount on growth of S. cerevisiae and bioethanol production were evaluated. The obtained results indicated that growth of S. cerevisiae (manifested as turbidity intensity) in the samples containing 0, 5, 10, 15, 20 and 25 μg sodium selenite, during aerobic process, was 0.1707, 0.1678, 0.1679, 0.1664, 0.1627 and 0.160% a.u./h (after 14 h incubation), respectively. Statistical analysis based on compression test indicated that there were insignificant (p > 0.05) differences between growth rate of the yeast in the fermented samples containing S. cerevisiae and 5 to 25 μg selenium salt. Response surface methodology was utilized to evaluate effects of two fermentation parameters namely, amount of selenium (5-25 μg) and substrate brix (10-25°Bx) on the concentration (g/L) of produced bioethanol. Obtained results revealed that maximum bioethanol concentration (55 g/L) was achieved using 15 μg selenium and molasses with 25°Bx. Furthermore, results have also indicated that, without using selenium and using molasses with 25°Bx, bioethanol with concentration of 29 g/L was produced.

High-pressure technology for Sargassum spp biomass pretreatment and fractionation in the third generation of bioethanol production

2021 ◽  
Vol 329 ◽  
pp. 124935
Author(s):  
E. Aparicio ◽  
Rosa M. Rodríguez-Jasso ◽  
César D. Pinales-Márquez ◽  
Araceli Loredo-Treviño ◽  
Armando Robledo-Olivo ◽  
...  
Keyword(s):  
High Pressure ◽  
Biomass Pretreatment ◽  
Third Generation ◽  
Bioethanol Production ◽  
The Third

scholarly journals Optimization of Bioethanol Synthesis from Durian Seeds Using Saccharomyces Cerevisiae in Fermentation Process

2020 ◽  
Vol 9 (1) ◽  
pp. 36-46
Author(s):  
Masturi Masturi ◽  
Dante Alighiri ◽  
Pratiwi Dwijananti ◽  
Rahmat Doni Widodo ◽  
Saraswati Putri Budiyanto ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Vacuum Distillation ◽  
Alternative Energy ◽  
Fermentation Process ◽  
Agricultural Crops ◽  
Bioethanol Production ◽  
Seed Flour ◽  
Fibrous Cellulose ◽  
Durian Seed ◽  
Batch Vacuum

Bioethanol is an alternative energy of environmentally friendly as a substitute for petroleum. Sucrose, starch, and fibrous cellulose (lignocellulose) are the main ingredients for bioethanol production. The material is very easy and abundant to get from the waste of agricultural crops. One of these agricultural wastes in Indonesia that have not been used optimally is durian seeds. Durian seeds only become waste and are not commercially useful, even though they contain high carbohydrates, which is possible as a potential new source for bioethanol production. In this work, an experimental study was conducted on bioethanol synthesis from durian seeds through fermentation by Saccharomyces cerevisiae yeast in aerobic fermenter. The process for the production of starch-based bioethanol includes milling, hydrolysis, detoxification, fermentation, and distillation. At the stage of fermentation, variations in the duration of fermentation were applied for 1-11 days. Carbohydrates contained in durian seed flour are 11.541%, which is the largest content. The highest result of ethanol content is 14.72 % (v/v) in 9 day fermentation periods by using Saccharomyces cerevisiae in aerobic conditions. Distillation to enrich bioethanol was carried out by batch vacuum distillation at 68°C for ± 180 minutes and produced bioethanol with a purity of 95%.

scholarly journals Mass Balance Analysis of Bioethanol Production from Petai Peel (Parkia speciosa) through Enzymatic Process

2021 ◽  
Vol 2 (02) ◽  
pp. 29-32
Author(s):  
Erwan Adi Saputro ◽  
Rachmad Ramadhan Yogaswara ◽  
Nerissa Arviana ◽  
Luckyana Aini ◽  
A.R Yelvia Sunarti ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mass Balance ◽  
Fermentation Process ◽  
Fossil Fuel ◽  
Initial Material ◽  
Bioethanol Production ◽  
Enzymatic Process ◽  
Good Opportunity ◽  
Balance Analysis ◽  
Mass Balance Analysis

The consumption of fuel for transportation is increasing during the last decade. Bioethanol is one of the renewable energy has a good opportunity to be applied when the lack of fossil fuel. Bioethanol is derived from the lignocellulose substance through a fermentation process. In this research, the lignocellulose came from the petai peel (Parkia speciosa). The peel was hydrolyzed using an enzyme and continuously fermented for 5 days. The aim of this research is to analyst the mass balance of the bioethanol production from petai peel (Parkia speciosa) through the enzymatic process. The enzyme used in this research are alfa amylase (10 ml) and glucoamylase (10 ml), also Saccharomyces cerevisiae used in the fermentation process. The result shows that the initial material of petai peel was 57 grams will produce bioethanol around 14 grams.

scholarly journals Valorization of Typha Australis Stems in Bioethanol Production Using Enzymatic Hydrolysis and Biofermentation

2019 ◽  
pp. 90-95 ◽  
Author(s):  
Cosme Sagbo Kouwanou ◽  
Cokou Pascal Agbangnan Dossa ◽  
Euloge Sènan Adjou ◽  
Mossi Issiakou ◽  
Valentin Dieudonné Wotto ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzymatic Hydrolysis ◽  
Fermentation Process ◽  
Second Generation ◽  
Great Variability ◽  
Bioethanol Production ◽  
Kinetics Parameters ◽  
Second Generation Bioethanol ◽  
Typha Australis ◽  
Active Dry Yeast

The present study aims to valorize the Typha australis stems, through second-generation bioethanol production using enzymatic hydrolysis and fermentation. The monitoring of fermentation kinetics parameters, such as pH, density, length of fermentation, and the Brix, indicated a great variability of these parameters during the fermentation process of the must with three Saccharomyces cerevisiae strains, such as Angel brand Thermal-tolerant alcohol active dry yeast, Angel brand super alcohol active dry yeast and Angel super alcohol active dry yeast in the presence of urea (CON2H4) used as a growth factor. The distillation of musts after fermentation has yielded ethanol extraction rate (% v / v at 20 ° C) between 4.95and 44.93 after fractional distillation. The best performance in ethanolic bioconversion was recorded with Angel brand super alcohol active dry yeast. This Saccharomyces cerevisiae strains could be used as effective ferments, in perspective of intensive production of second-generation bioethanol with Typha australis stems.

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