288 Inclusion Effects of Quebracho (Schinopsis Balansaie) Extract and Active Dry Yeast (Saccharomyces Cerevisiae) in Beef Cattle Limit-fed a Grower Ration

Plant secondary metabolites (PSM) and yeast supplemented in growing cattle have been reported as improving dry matter digestibility (DMD) and reducing enteric methane emissions. This study aimed to evaluate the effect of combined supplementation with condensed tannin (CT; Schinopsis balansae) extract and active dry yeast (ADY; Saccharomyces cerevisiae) on fermentation dynamics, utilizing in vitro gas production (IVGP) technique. A 2 × 2 Latin square design was used to study fermentation patterns of four dietary treatments (CON- no CT and no ADY, ADY alone, CT alone or combined CT + ADY). Animals received daily CT at 1% DM and 10 g of ADY, respectively. On d 0, 7, 14, 21, 28, and 35 rumen inoculum was collected from 23 fistulated steers (284.3 ± 4.1 kg) four hours post-feeding. Samples were incubated under anaerobic conditions at 39oC for 48 h with 200 mg of a grower diet (14.8% CP, 40.6% NDF, 88.5% DM). Gas parameters were analyzed using a mixed linear statistical model. There was a day effect for total gas production (TGP; P < 0.001), non-fiber carbohydrate degradation (P = 0.031) and fractional degradation assuming an asymptote model (P = 0.015). Both asymptote and non-fiber fractional degradation rate estimates had an interaction between Day × TRT (P = 0.001 and 0.0104, respectively). Data were analyzed using polynomial contrasts showed a difference in non-fiber fractional rate of fermentation for CON × CT and CT × ADY (P = 0.052 and 0.054, respectively). This was also true if an asymptote model was assumed (P = 0.0 34 and 0.034, respectively). We concluded that combined supplementation of CT and ADY exhibited similar IVGP trends over time, this may be because animals only received a grower diet at 1.5% shrunk BW. Future studies should investigate the impact of combined supplementation on varying levels of concentrate diets.

The effects of feeding benzoic acid and/or live active yeast (Saccharomyces cerevisiae) on beef cattle performance, feeding behaviour, and carcass characteristics

Fifty-nine Angus-cross finishing steers were used to evaluate benzoic acid, active dry yeast (Saccharomyces cerevisiae), or a combination of benzoic acid and active dry yeast when supplemented in a high-grain finishing diet on live animal performance, feeding behaviour, and carcass characteristics. Steers were fed a high-grain diet for the final 106 d of finishing. Treatments were; no additional supplementation (CON), 0.5% benzoic acid (ACD), 3 grams per head per day active dry S. cerevisiae (YST), or both 0.5% benzoic acid and 3 grams/head per day S. cerevisiae (AY). Steers were weighed every 14 d, and ultrasound was performed for rib and rump fat thickness at the beginning (d 1), middle (d 57), and end (d 99) of the experiment. Insert feeding stations were used to collect individual feeding behaviour data and DMI daily throughout. Blood samples were collected on d 21-22 and d 99-101 to assess plane of nutrition and metabolism. Ruminal fluid samples were collected by oral gavage 4 weeks prior to slaughter. Carcass characteristics were examined at a federally inspected slaughter facility. Data were analyzed using PROC GLIMMIX of SAS with initial body weight (BW) as a covariate. Benzoic acid supplementation increased (P = 0.002) overall dry matter intake (DMI) compared to YST and CON steers, which may be due to a faster eating rate (P ≤ 0.008). Animal performance parameters (BW, average daily gain, feed conversion, and ultrasound fat depth) were not different (P ≥ 0.11) among treatment groups. Aspartate aminotransferase concentration was greatest (P ≤ 0.01) for YST steers, which may have been reflected in numerically greater liver abscesses. Carcass traits did not differ (P ≥ 0.33) among treatment groups. Ruminal pH was greater (P = 0.006) for ACD steers than AY steers (pH of 6.16 vs. 5.66, respectively), which indicated that there may be an interactive effect between benzoic acid and active dry yeast. To summarize, steers fed a high-grain finishing diet supplemented with benzoic acid, active dry yeast, or both benzoic acid and active dry yeast had similar growth performance and carcass characteristics compared to those without supplementation. However, the addition of benzoic acid alone increased DMI, variation in DMI, eating rate, and ruminal pH. Future studies are warranted to further investigate the impacts of benzoic acid on the ruminal environment of feedlot cattle

FORMATION OF THE QUALITY OF UNFORTIFIED WINE MATERIALS OBTAINED FROM BLACK ELDERBERRIES

The use of local non-conventional raw materials will allow significantly enriching unfortified fruit wines with ascorbic acid and phenolic substances and make it possible to obtain products with increased biological value. The paper considers black elderberries grown in the forest steppe zone of Ukraine. It has been determined that they contain 12–13% of soluble solids, 6.9–8.1 of sugars, 0.93–1.2% of titratable acids, 33.4–53.1 mg/100 g of ascorbic acid, and 2331–3888 mg/100 g of phenolic substances. The pressing process and its dependence on the method of treating berries have been studied. It has been proved that juice extraction from black elderberries is the highest when they are pretreated with heat at 98±2°C for 3–5 minutes, with 15% of water added. Depending on the treatment method, juices retain ascorbic acid (15.8–33.4 mg/100 g) and phenolic substances (2538–3888 mg/100 g), which indicates their high biological value. Juices like these can be used to improve the biological composition of blended juices and wines. To ferment high-sugar black elderberry must, active dry yeast was used, namely the yeast races EC-1118 of the genus Saccharomyces bayanus (France) and ENSIS LE-1 or ENSIS LE-5 of the genus Saccharomyces cerevisiae (Spain). The musts were fermented for 36–62 days. The period of vigorous fermentation coincides with the period of initial fermentation and ends in 9–10 days, with accumulation of 12–14% of ethanol by volume. Unfortified wine materials obtained from black elderberries contain 22–38.3 mg/100 g of ascorbic acid (its content in wine materials is by 7.8–49.55% lower than in fresh berries) and 1750–3510 mg/100 g of phenolic substances (which is lower by 9.7–39.6%, as compared with fresh elderberries). The active acidity of black elderberry wine materials is 3.90–4.09 pH units, depending on the yeast race. A similar difference has been found for the redox potential (160–176 mV), which indicates that the wine materials from black elderberries are low-oxidised. The intensity of colour of the wine materials obtained from black elderberries (D420 + D520) varied from 3.08 to 3.20, and the colour shade from 0.88 to 0.92, which is typical of young red wines. These wine materials can be used to increase the quality and biological value of blended wines.

Inoculation of grape musts with single strains of Saccharomyces cerevisiae yeast reduces the diversity of chemical profiles of wines

Anecdotal evidence suggests that spontaneous alcoholic fermentation of grape juice is becoming a more popular option in global wine production. Wines produced from the same grape juice by inoculation or spontaneous fermentation usually present distinct chemical and sensorial profiles. Inoculation has been associated with more similar end-products, a loss of typicity, and lower aroma complexity, and it has been suggested that this may be linked to suppression of the local or regional wine microbial ecosystems responsible for spontaneous fermentations. However, whether inoculated fermentations of different juices from different regions really end up with a narrower, less diverse chemical profile than those of spontaneously fermented juices has never been properly investigated. To address this question, we used grape juice from three different varieties, Grüner Veltliner (white), Zweigelt (red), and Pinot noir (red), originating from different regions in Austria to compare spontaneous and single active dry yeast strains inoculated fermentations of the same grape samples. The chemical analysis covered primary metabolites such as glycerol, ethanol and organic acids, and volatile secondary metabolites, including more than 40 major and minor esters, as well as higher alcohols and volatile fatty acids, allowing an in depth statistical evaluation of differences between fermentation strategies. The fungal (mainly yeast) communities throughout fermentations were monitored using automated ribosomal intergenic spacer analysis. The data provide evidence that inoculation with single active dry yeast strains limits the diversity of the chemical fingerprints. The fungal community profiles clearly show that inoculation had an effect on fermentation dynamics and resulted in chemically less diverse wines.

EFFECT OF CHICKEN MANUREFERTILIZATION, ACTIVE DRY YEAST FOLIAR SPRAY CONCENTRATIONS AND THEIR INTERACTION ON LAVANDER (LAVANDULA OFFICINALIS, CHAIX) PLANTS GROWTH AND PRODUCTIVITY

A field experimental laid out at a completely randomized block design at the experimental farm of Fac. of Environ. Agric. Sci., Arish Univ., El-Arish, North Sinai Governorate, Egypt during the two successive summer seasons of 2017/2018 and 2018/2019, to study the effect of chicken manure (ChM) as N fertilizer source, dry yeast spraying concentrations and their interaction on vegetative, yield and chemical composition of lavender (Lavandula officinalis). Results showed that fertilization lavender plants with 100 % from the recommended N rate as chicken manure (ChM) recorded the maximum growth, yield and chemical constitutes of lavender. Moreover, application of dry yeast at 200 ppm increased significantly lavender growth, yield and chemical constitutes. For the interaction between chicken manure (ChM) as N fertilizer source and dry yeast concentrations the same trend were observed when plants fertilized with 100 % from the recommended N rate as chicken manure (ChM) and sprayed with 200ppm dry yeast.

Application of Autochthonous Yeast Saccharomyces cerevisiae XG3 in Treixadura Wines from D.O. Ribeiro (NW Spain): Effect on Wine Aroma

Yeast plays an essential role in winemaking. Saccharomyces cerevisiae strains involved in fermentation determine the chemical and sensory characteristics of wines. S. cerevisiae XG3, isolated in Galicia (NW Spain), has desirable oenological potential, which has been proved at a pilot scale to produce quality wines. This study applies XG3 as active dry yeast at an industrial scale for Treixadura wine elaboration, and compares it with commercial yeast and spontaneous fermentation within three wineries included in Denomination of Origin Ribeiro over two vintages. Fermentations are monitored using conventional methods, and microbiological implantation controls are carried out by mtDNA-RFLPs analysis. Wine basic chemical parameters are determined using OIV official methodology, and volatile aroma compounds are determined by GC-MS. Finally, wine sensory analysis is also performed. S. cerevisiae XG3 shows an acceptable implantation ability—as compared to commercial control strains. The wines from XG3 have a higher total acidity and lower alcohol content. Their volatile composition differs from control wines, since XG3 produces significantly higher concentrations of acetates, volatile acids, esters and volatile phenols, depending on the vintage and winery. However, lower differences are perceived at the sensory level, where fruity and floral descriptors are perceived by the panellists in XG3 wines. Therefore, XG3 constitutes an alternative to differentiate Treixadura wines.