scholarly journals Innovative approaches to identifying the relationship between the distribution of the concentration of enzymes in the blood serum and skeletal muscle tissue of rats

2019 ◽  
Vol 135 ◽  
pp. 01098
Author(s):  
Pavel Boriskin ◽  
Olga Gulenko ◽  
Anatoliy Deviatkin ◽  
Rufia Karimova ◽  
Olga Pavlova
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Blood Serum ◽  
Muscle Tissue ◽  
Functional Activity ◽  
Active Oxygen Species ◽  
Free Radical Oxidation ◽  
The Body ◽  
Skeletal Muscle Tissue ◽  
Radical Oxidation

One of the reasons for the increase in disability and mortality among the working population is cardiovascular diseases. Long-term studies of the nature of diseases belonging to this group hypertension, renovascular and malignant arterial hypertension, dyslipidemia, diabetic and dilatational cardiomyopathy, atherosclerosis, ischemic heart disease show that the main cause of pathogenesis is the formation of oxidative stress under the influence of internal and external environmental factors. Mismatch between the processes of free-radical oxidation caused by such active oxygen species as superoxide-anion radical, hydroxide-radical radical, lipid radical, hydrogen peroxide, peroxynitrite, chloric acid and the capabilities of the antioxidant system leads to a disruption of the functional activity of cell structures, displacement of homeostatic balance in the body. Despite the rather deep study of cardiovascular system pathologies, data on the functional activity of myocardial cells under destructive effects and oxidative stress are still insufficient.The article presents the study of the interrelation of the concentration distribution of enzymes of the lipidantioxidant peroxidation system in blood serum and skeletal muscle tissue of white non-pedigree rats. By means of Spearman, Gamma and Kendel Tau correlation coefficients it was revealed a reliable presence of a weak force of inverse correlation between the concentration of catalase in blood serum and skeletal muscle (Spearman R = -0.21 at p < 0.011749, Gamma = -0.14 at p < 0.010977; Kendall Tau = -0.14 at p < 0.010977).

scholarly journals Study of superoxiddismutase activity dynamics in rat skeletal muscle tissue under mechanical influence on hemato-ophthalmic barrier

2020 ◽  
Vol 210 ◽  
pp. 06013
Author(s):  
Anatoly Devyatkin ◽  
Olga Pavlova ◽  
Olga Gulenko ◽  
Pavel Boriskin ◽  
Olga Tulaeva
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Mechanical Action ◽  
The Body ◽  
Skeletal Muscle Tissue ◽  
Male Rats ◽  
Rat Skeletal Muscle ◽  
Sod Activity ◽  
Barrier Materials

Hemato-ophthalmic barrier is one of the mechanisms of body resistance. One of the complications of mechanical trauma of the eye and violation of the hemato-ophthalmic barrier is the emergence of oxidative stress on the background of the general inflammatory process. Normally, oxidative stress in the skeletal muscle tissue is not a damaging agent, but when intensified by other factors, it promotes pathological changes in the body. Objective: to study the dynamics of superoxiddismutase (SOD) activity in rat skeletal muscle tissue under oxidative stress caused by mechanical action on the hemato-ophthalmic barrier. Materials and methods: The study was carried out on pedigree matured male rats in the amount of 150 pieces. The activity of SOD in skeletal muscle tissue was studied before the experiment, as well as on the 1st, 3rd, 5th, 7th and 14th day of the experiment using the standard technique of V.S. Gurevich. The obtained digital material was subjected to statistical processing by means of non-parametric statistical analysis. Conclusion: SOD activity in rat skeletal muscle tissue under oxidative stress caused by mechanical action on hemato-ophthalmic barrier is most effectively stabilized in standard therapy of mechanical eye injury with the addition of quercetin in the form of injections.

Gender differences in biochemical measurement reflecting the state of free-radical oxidation and antioxidant protection among workers in metallurgical production

2019 ◽  
Vol 1 (10) ◽  
pp. 877-881
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Gender Differences ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Metallurgical Production ◽  
Production Factors

Introduction. To date, age and sex differences have been established for many biochemical parameters. Gender differences in indicators for systems such as antioxidant, thiol-disulfide, oxidative stress and inflammation systems are absent or under study.The aim of the study was to identify gender differences in biochemical parameters reflecting the functioning of antioxidant systems of the body and free radical oxidation in workers of metallurgical production, in contact with harmful production factors.Materials and methods. The blood of men and women working at the metallurgical enterprise of the Nizhny Novgorod region (n=80) under the influence of a complex of physical and chemical production factors was studied. Total oxidative stress, total antioxidant capacity of serum, glutathione levels were studied by photometric biochemical methods. Levels of C-reactive protein and 8-hydroxy–2-deoxyguanosine were studied by ELISA.Results. The average amount of peroxides in the serum of women exceeded 1.6 times this figure in men. In the group of men, the content of 8-Ondg was higher by 26% (p=0.012), the level of GS-by 12% (p=0.019), the activity of SOD — by 1.5–2 times (p=0.0001), the level of CRP — by 2 times (p=0.008) compared to similar indicators in women.Conclusions. Studies of gender differences in workers under the influence of harmful production factors will allow more effective approach to the etiology, treatment and prognosis of production-related diseases. Indicators of oxidative stress and antioxidant protection can be indicators of the health of workers under the influence of harmful industrial factors and be important in the prevention of diseases associated with oxidative stress.

scholarly journals Peculiarities of lipid peroxidation processes in the body of cats with urinary tract diseases and their correction with commercial feeds

2021 ◽  
pp. 37-40
Author(s):  
Olga Andreevna Vorontsova ◽  
Nikolay Alexandrovich Pudovkin ◽  
Vladimir Vasilievich Salautin ◽  
Sergey Dmitrievich Klyukin
Keyword(s):  
Urinary Tract ◽  
Blood Serum ◽  
Catalase Activity ◽  
Free Radical Oxidation ◽  
The Body ◽  
Diene Conjugates ◽  
Radical Oxidation ◽  
Mda Content ◽  
Peroxidation Processes ◽  
Commercial Feeds

The article presents the results of studies on the effect of commercial food on the processes of free radical oxidation of lipids in the body of cats with urinary tract pathology. It has been found that the concentration of diene conjugates in the blood serum of sick animals increases significantly by 15.1 - 36.7% relative to healthy ones. The MDA content in the blood serum of healthy animals was 6.05 ± 0.28 nmol / g. In animals with urolithiasis, this indicator increased by about 2 times. When feeding with commercial food and homemade food, LPO processes normalize, which is most pronounced on the 21st day. The average activity of catalase in the blood serum of sick animals was increased from 30.6% to 40.2% relative to healthy ones (15.15 ± 0.61 ?mol / ml). After feeding with therapeutic and prophylactic fodders, by the 7th day, the catalase activity did not significantly decrease compared to the initial level, but remained higher than in control animals by 19.6% - 37.1%. On the 14th day, the catalase activity did not change significantly compared with the 7th day and was higher than the control values by 21.5% - 27.5%.

scholarly journals Quercetin improves myocardial redox status in rats with type 2 diabetes

2021 ◽  
Vol 55 (3) ◽  
pp. 142-152
Author(s):  
Nataliia I. Gorbenko ◽  
Oleksii Yu. Borikov ◽  
Tetiana V. Kiprych ◽  
Olha V. Ivanova ◽  
Kateryna V. Taran ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Free Radical Oxidation ◽  
Redox Status ◽  
Diabetic Rats ◽  
The Body ◽  
Dependent Manner ◽  
Citrate Buffer ◽  
Radical Oxidation

Abstract Objective. Emerging data indicate that oxidative stress is closely associated with the pathogenesis of cardiovascular disease in type 2 diabetes mellitus (T2DM). The present study aimed to assess the effect of the most abundant flavonoid in the human diet quercetin (Q) on the myocardial redox status in rats with T2DM. Methods. T2DM was induced in male Wistar rats by a high caloric diet (for 14 weeks) and two streptozotocin (25 mg/kg b.w.) injections applied in four weeks of the diet, once a week for two weeks. The Q was administered intragastrically by gavage in a dose of 10 or 50 mg/kg of the body weight for 8 weeks starting from the 8th day after the last streptozotocin injection. The control rats received citrate buffer and seven days after the last STZ injection, basal glucose levels were measured in all animals. Results. Administration of Q increased insulin sensitivity in diabetic rats with more pronounced effect at a dose of 50 mg/kg b.w. The Q also decreased free radical oxidation in the heart mitochondria of diabetic animals, thus limiting the formation of advanced oxidation protein products in a dose-dependent manner and normalized the activity of antioxidant enzymes (superoxide dismutase, glutathione peroxidase, glutathione reductase) in cardiac mitochondria independently of the dose used. In addition, the Q in both doses prevented the development of oxidative stress in the T2DM rats cardiomyocytes by reducing NADPH oxidase and xanthine oxidase activities. Conclusions. The findings demonstrate that Q in both doses 10 mg/kg and 50 mg/kg can protect from the development of oxidative stress in cardiomyocytes in the diabetic rats. The present data indicate that the use of Q may contribute to the amelioration of cardiovascular risk in patients with T2DM.

scholarly journals Iron Supplementation in Experimental Hyperthyroidism: Effects on Oxidative Stress in Skeletal Muscle Tissue

2004 ◽  
Vol 45 (3) ◽  
pp. 413 ◽  
Author(s):  
Hakki Oktay Seymen ◽  
Sabiha Civelek ◽  
Arzu Seven ◽  
Günnur Yiğit ◽  
Hüsrev Hatemi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Iron Supplementation ◽  
Skeletal Muscle Tissue ◽  
Experimental Hyperthyroidism

scholarly journals PO-167 Effects of Hydrogen-rich Water on Rat Skeletal Muscles' Oxidative Damage and Autophagy Induced by Repeated Exhaustive Exercise

2018 ◽  
Vol 1 (4) ◽  
Author(s):  
Zujie Xu ◽  
Hongdi Gu ◽  
Zhenjun Tian
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Protein Expression ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue ◽  
Exhaustive Exercise ◽  
Ultrastructural Changes ◽  
Sod Activity ◽  
Hydrogen Water ◽  
Mda Content

Objective This study was carried out to detect the effects of hydrogen-water intervention on oxidative stress and cell autophagy in skeletal muscle of rats with repeated exhaustion.  Methods 30 male SD rats in the age of 3 months, weighing 180-210g, were randomly divided into control group (C), repeated exhaustion group (EX), and the repeated exhaustion with hydrogen intervention group (EH), 10 rats in each group. The EX and EH groups were subjected to a four-weeks of repeated-exhaustive exercise. The initial speed of the exercise was 15 m/min, and increasedby 5 m/min every 5 min, the final speed is 35m/min until  exhaustion, 5 d/wk, with a total of 4 wk. In EH group, hydro-water was given to rats 30 mins before exercise. The ultrastructural changes of skeletal muscle were observed by using a transmission electron microscopy. Activity of SDH and CK in serum and SOD activity, MDA content and T-AOC level in skeletal muscle tissue were detected. Western blotting was used to detect the proteins expression of autophagy related proteins in skeletal muscle, mTOR, p-mTOR, LC3B-2 and P53.  Results Compared with the EX group, in the EH group, the ultrastructural damage and mitochondrial swelling were significantly reduced, and the time of exhaustion was significantly prolonged (p<0.05), Serum SDH activity increased significantly (p<0.05), CK activity decreased significantly (p<0.05), and skeletal muscle tissue SOD activity and total antioxidant capacity significantly increased (p<0.05), MDA content decreased significantly (p<0.01), mTOR and p-mTOR protein expression was significantly increased(p<0.05), the LC3B-2 and P53 protein expression was significantly lower (p<0.05). Conclusions Hydrogen water intervention could significantly improve repeatedly exhaustion exercise result in rat skeletal muscle injury, oxidative stress and cell ultrastructure damage excessive autophagy, improving oxidation resistance of the skeletal muscle and exercise endurance.

CYTOMORPHOLOGICAL CHANGES IN THE CARDIAC AND SKELETAL MUSCLE TISSUE OF RABBITS IN LEAD INTOXICATIONS

2020 ◽  
Author(s):  
A.I. Golubev ◽  
◽  
M.A. Kupriyanova ◽  
M.M. Salnikova ◽  
V.R. Saitov ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Morphometric Analysis ◽  
Natural Environment ◽  
Lead Acetate ◽  
The Body ◽  
Skeletal Muscle Tissue ◽  
Ultrastructural Changes ◽  
Natural Ecosystems ◽  
Visual Disorders

The pollution and accumulation of lead and its compounds in the natural environment every year poses an increasing threat to human health and natural ecosystems. This problem is the most serious in megacities. The established criteria and the results of practical studies indicate lead as one of the most dangerous ecotoxicants. In the present work, the effect of lead acetate on the body of productive animals was analyzed, and cytomorphological and ultrastructural changes in the heart and striated (skeletal) muscle tissue were revealed. Visual disorders are confirmed by morphometric analysis.

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