Profile of Haematology and Biochemistry of Aceh Polled and Horned Cow

As one of the Local wisdom in Aceh, farmer prefers raising polled cow than horned cow for breeding purposes. They believe pole cow is more resilient in harsh conditions and reproductively better than the horned cow. The aim of this study was to evaluate blood profile and blood biochemistry of the aceh polled and horned cow. The blood profile examined includes erythrocyte value, erythrocyte indices MCV (Mean Corpuscular Volume), MCH (Mean Corpuscular Hemoglobin), MCHC (Mean Corpuscular Hemoglobin Concentration), thrombocyte, leukocyte, hematocrit, and hemoglobin, while blood biochemistry includes protein total, glucose, cholesterol, and ureum. Blood was collected from 15 aceh horned and 15 pole cow raised at Research Institute of Improved Livestock and Livestock Fodder (BPTU HPT) Indrapuri, Aceh Besar regency, province of Aceh, Indonesia. The cows were clinically healthy and aged 1,5-2,5 years old. Blood profile was measured using a hematology analyzer and blood biochemistry was measured using a Yuesen Med and Spectrophotometer. Based on the statistic analysis, blood profile and blood biochemistry of the aceh horned and polled cow on all parameters showed no significant difference (p>0,05). It is therefore concluded that there are no different value of blood profile and blood biochemistry between polled and horned cow.

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Hematological changes in deer mice acclimated at different ambient temperatures

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1960.198.1.195 ◽

1960 ◽

Vol 198 (1) ◽

pp. 195-199 ◽

Cited By ~ 5

Author(s):

John A. Sealander

Keyword(s):

Hemoglobin Concentration ◽

Deer Mice ◽

Mean Corpuscular Hemoglobin ◽

Mean Corpuscular Hemoglobin Concentration ◽

Corpuscular Hemoglobin ◽

Ambient Temperatures ◽

Significant Difference ◽

Similar Temperature ◽

The Mean ◽

Hematological Changes

Deer mice ( Peromyscus maniculatus and Peromyscus leucopus) were acclimated at temperatures of 5° ±0.5°C and 30°±0.5°C for various intervals with periods at 20°±0.5°C preceding the cold and warm acclimation periods. Hemoglobin concentrations, hematocrits and erythrocyte diameters were measured on a group of nonsplenectomized mice and on another group of splenectomized mice which were subjected to similar temperature conditions. It was found that there were significant increases in hemoglobin and hematocrit values with acclimation at 5°C, which were more marked in the splenectomized mice. Differences in hemoglobin concentrations and hematocrits were significant between 5° and 30°C, but differences were not as apparent between mice at 30°C and those exposed at 20°C. There were no significant changes in erythrocyte diameters, although the mean corpuscular hemoglobin concentration was significantly lower in all of the splenectomized mice as opposed to the nonsplenectomized ones. It is suggested that splenectomy in some way enhances erythopoiesis in response to low temperature since there was no significant difference in hematocrits of nonsplenectomized and splenectomized mice exposed at 20°C prior to cold acclimation.

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Inaccuracy in automated measurement of hematocrit and corpuscular indices in the presence of severe hyperglycemia

Blood ◽

10.1182/blood.v57.6.1065.1065 ◽

1981 ◽

Vol 57 (6) ◽

pp. 1065-1067 ◽

Cited By ~ 21

Author(s):

JA Strauchen ◽

W Alston ◽

J Anderson ◽

Z Gustafson ◽

LF Fajardo

Keyword(s):

Blood Glucose ◽

Mean Corpuscular Volume ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Volume ◽

Automated Measurement ◽

Mean Corpuscular Hemoglobin Concentration ◽

Corpuscular Hemoglobin ◽

Hyperosmolar Glucose

Abstract Because we recently observed two patients with severe diabetic hyperglycemia and spuriously elevated electronically determined hematocrit and mean corpuscular volume (MCV), we investigated the effect of hyperglycemia on two popular automated hematology systems, the Coulter S and Ortho ELT-8. Marked hyperglycemia (blood glucose 800-- 2000 mg/dl) caused consistent overestimation of the electronically determined MCV compared to that derived from a simultaneous spun microhematocrit. The resultant overestimation and underestimation, respectively, of the derived values for hematocrit and mean corpuscular hemoglobin concentration may be clinically misleading. The mechanism of MCV elevation in hyperglycemia appears to be swelling of hyperosmolar glucose “loaded” erythrocytes when diluted into “isotonic” counting medium. This effect is readily circumvented by determination of a spun microhematocrit.

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Impacts of Mycoplasma loads and lung lesions on immune and hematological statuses of pigs in an eight-breed cross heterogeneous population

Journal of Animal Science ◽

10.1093/jas/skaa235 ◽

2020 ◽

Vol 98 (8) ◽

Author(s):

Qing Zhang ◽

Jing Li ◽

Tao Huang ◽

Yifeng Zhang ◽

Wenwu Xu ◽

...

Keyword(s):

Blood Cell ◽

Neutrophil Count ◽

Lymphocyte Count ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Volume Distribution ◽

Mean Corpuscular Hemoglobin Concentration ◽

Corpuscular Hemoglobin ◽

Distribution Width ◽

Lung Lesions

Abstract Developments of pulmonary diseases, often accompanied by infections of bacteria, severely affect the meat production and welfare of pigs. This study investigated 307 pigs at age of 240 d from an eight-breed cross reared under standardized housing conditions for associations among the extent of lung lesions, bacteria load inferred from 16S rRNA sequencing of bronchoalveolar lavage fluid, as well as 57 immune cells and 25 hematological traits. We showed that the pigs under study suffered substantial and varied lung lesions, and the Mycoplasma is the most associated bacteria genera. At a false discovery rate of 0.05 (FDR < 0.05), the severity of lung lesions were significantly associated with greater CD8+ to CD3+ cell ratio, neutrophil-to-lymphocyte ratio (NLR), and standard deviation of red blood cell volume distribution width (RDW-SD), and lower CD4−CD8−/CD3+, CD3+CD4−CD8−/PBMCs (peripheral blood mononuclear cells) and CD14−CD16−/PBMCs cell ratios, mean corpuscular hemoglobin concentration, lymphocyte count, and lymphocyte count percentage, reflecting an status of inflammation, immune suppression, and hypoxia of the pigs accompanying the progression of the lung lesions. The Mycoplasma abundance showed positive correlations with neutrophil count, neutrophil count percentage, NLR, monocyte count, coefficient of variation in red blood cell volume distribution width , and RDW-SD, and negative correlations with mean corpuscular hemoglobin concentration, lymphocyte count, and lymphocyte count percentage; these correlations are largely consistent with those of lung lesions, supporting the comorbidity of lung lesions and Mycoplasma infection. We also observed nonlinear associations that sharp increases in neutrophil count and neutrophil count percentage occurred only when Mycoplasma abundance raised above the population-average level. The results provide helpful insights into the changes of host immune status in response to Mycoplasma relevant lung diseases in pigs.

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HEMATOLOGICAL CHARACTERIZATION OF BETA-THALASSEMIA IN SUDANESE PATIENTS

Innovare Journal of Medical Sciences ◽

10.22159/ijms.2020.v8i3.36436 ◽

2020 ◽

pp. 5-7

Author(s):

RABAB HASSAN ELSHAIKH ◽

SANAA ELFATIH HUSSEIN

Keyword(s):

Iron Deficiency ◽

Hemoglobin Concentration ◽

Mean Value ◽

Beta Thalassemia ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Significant Difference ◽

Hematological Analysis ◽

The Mean

Thalassemia is common inherited disorder among humans, and they represent a major public health problem in many areas of the world. The study aimed to the measurement of hematological characterization of beta-thalassemia in Sudanese patients. Blood samples from 61 beta-thalassemic patients were collected after written consent form obtained from all participants. The frequency of adults (>18 years) was 45 (73.8%) and children’s (<18 years) was 16 (26.2%); the frequency of male was 27 (44.3%) and 34 were female (55.7%). Hemoglobin estimation and red cell indices were carried out using the automatic blood cell counter Sysmex K × 21N. The results showed that Hb and RBCs indices were varied between mild to moderate and severe decreasing, hemoglobin concentration (Hb) with the mean value of 9.6 g/dL, with minimum value of 6.1 g/dl and maximum of 11.9 g/dl, while RBCs were increased in all patients, mean value 5.2 c/l, mean corpuscular volume mean was 58.9 fl, hematocrit was 30.4, mean corpuscular hemoglobin (MCH) 18.8 pg, mean corpuscular hemoglobin concentration (MCHC) was 31.7pg, and RDW was 18.8%. The method used for hemoglobin electrophoresis was capillary electrophoresis, Hb pattern shows increased HbA2 and HbF, the mean of HbA is 78.3%, HbF is 2.3%, and HbA2 is 6.5% with the min. value of 3.6% and max. of 12.2%. While the mean of serum iron was 82.75 μg/dl, 7 patients showed low level, 19 high level, and 35 were normal level. Comparison of hematological analysis (HbA2) in thalassemic patients coexisted with iron deficiency and without result was insignificant difference (p=0.645), this result disagrees with references that say iron deficiency masking HbA2. Nevertheless, the association between HbA2 and HbF revealed a statistically significant difference (p<0.013) and HbA2 with Hb was insignificant (p=0.260).

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Blood Donation in Nigeria: Standard of the Donated Blood

Journal of Laboratory Physicians ◽

10.4103/0974-2727.105589 ◽

2012 ◽

Vol 4 (02) ◽

pp. 094-097 ◽

Cited By ~ 4

Author(s):

Nwogoh Benedict ◽

Awodu Omolade Augustina ◽

Bazuaye Godwin Nosakhare

Keyword(s):

Blood Donation ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Mean Cell Volume ◽

Benin City ◽

Cell Counts ◽

Significant Difference ◽

The Mean

ABSTRACT Objective: The objective was to determine the basic hematological parameters of remunerated blood donors in Benin City and to compare them with those of voluntary donors. Materials and Methods: This is a prospective study conducted in a tertiary health facility in Benin City. Pretransfusion samples were obtained from blood bags after gentle mixing and analyzed for hematological parameters. Samples were analyzed using the hematology autoanalyzer MODEL SYSMEX KN21. Result: A total of 215 samples were obtained comprising 160 remunerated (paid) and 55 voluntary donor samples. In the paid donors, the mean hemoglobin concentration (Hb) and hematocrit (HCT) 7.7±2.9 and 28.8±8.5 respectively. This was significantly lower than those of voluntary donors who had 13.9±1.2 and 42.2±3.3 with P < 0.001. The mean values of the red cell counts (RBC), white cell counts (WBC), mean cell volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were significantly lower in paid donors as P-values were <0.001. MCV was significantly low but not compared to the other parameters as P=0.04. There was no significant difference in the platelet count. Conclusion: Paid donors in Benin City have significantly lower hematological parameters than controls.

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Nilai Mean Corpuscular Hemoglobin Concentration, Mean Corpuscular Volume dan Mean Corpuscular Hemoglobin Darah Sapi Bali yang Dipelihara Berbasis Organik

Buletin Veteriner Udayana ◽

10.24843/bulvet.2021.v13.i01.p07 ◽

2021 ◽

pp. 39

Author(s):

I Gede Agus Eva Prawira Adinata ◽

Ni Ketut Suwiti ◽

Anak Agung Sagung Kendran

Keyword(s):

Mean Corpuscular Volume ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Volume ◽

Mean Corpuscular Hemoglobin Concentration ◽

Corpuscular Hemoglobin ◽

Corpuscular Haemoglobin ◽

Corpuscular Hemoglobin Concentration ◽

Hematology Analyzer ◽

Mean Corpuscular Haemoglobin

Telah dilakukan penelitian untuk mengetahui nilai hematologi MCHC (Mean Corpuscular Hemoglobin Concentration), MCV (Mean Corpuscular Volume) dan MCH (Mean Corpuscular Haemoglobin) darah sapi bali yang dipelihara berbasis organik. Sistem pemeliharaan berbasis organik adalah manajemen pemberian pakan yang berasal dari lingkungan serta tidak menggunakan pestisida atau zat kimia lainnya. Nilai MCV, MCH dan MCHC darah sapi bali diukur dengan Veterinary hematology analyzer. Hasil penelitian menunjukan, nilai dari MCHC sebesar 39,17%/dl, MCV sebesar 48,44 fl dan nilai dari MCH sebesar 18,69pg. Dapat disimpulkan rerata nilai tersebut masih dalam batas normal nilai indeks eritrosit sapi pada umumnya.

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Effect of Resistance Training on Hematological Blood Markers in Older Men and Women: A Pilot Study

Current Gerontology and Geriatrics Research ◽

10.1155/2009/156820 ◽

2009 ◽

Vol 2009 ◽

pp. 1-4 ◽

Cited By ~ 12

Author(s):

Florian Bobeuf ◽

Mélissa Labonté ◽

Abdelouahed Khalil ◽

Isabelle J. Dionne

Keyword(s):

Resistance Training ◽

Large Population ◽

Hemoglobin Concentration ◽

Blood Parameters ◽

Mean Corpuscular Hemoglobin ◽

Older Individuals ◽

Corpuscular Hemoglobin ◽

Distribution Width ◽

Men And Women ◽

Significant Difference

The aim of this study was to examine the effects of resistance training on hematological blood markers in older individuals. Twenty-nine men and women participated to this study. Subjects were randomized in 2 groups: (1) control (n=13) and (2) resistance training (n=16). At baseline and after the intervention, subjects were submitted to a blood sample to determine their hematological profile (red blood cells, hemoglobin, hematocrit, platelets, leukocytes, neutrophils, lymphocytes, monocytes, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red cell distribution width). At baseline, no difference was observed between groups. Moreover, we found no significant difference after the intervention on any of these markers. A 6-month resistance program in healthy older individuals seems to have no beneficial nor deleterious effects on hematological blood parameters. However, resistance training was well tolerated and should be recommended for other health purposes. Further studies are needed to confirm these results in a large population.

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Hematological and serum biochemical findings in clinical cases of cattle naturally infected with lumpy skin disease

The Journal of Infection in Developing Countries ◽

10.3855/jidc.5038 ◽

2015 ◽

Vol 9 (03) ◽

pp. 283-288 ◽

Cited By ~ 7

Author(s):

Sameeh M Abutarbush

Keyword(s):

Skin Disease ◽

Venous Blood ◽

Hemoglobin Concentration ◽

Viral Disease ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Creatinine Concentration ◽

Blood Profile ◽

Lumpy Skin Disease ◽

Serum Biochemical

Introduction: Lumpy skin disease (LSD) is an acute viral disease of cattle that is currently emerging in the Middle East region and poses a serious threat to Europe and the rest of the world. The objective of this study was to describe hematological and serum biochemical findings associated with natural clinical infection of LSD in cattle. Methodology: A total of 129 animals clinically infected with LSD were enrolled in the study. Venous blood sample were collected from study animals, and hematological and serum biochemical parameters were measured. Results: Leukocytopenia was found in 8.7%, while leucocytosis was found in 18.2% of affected cattle. Decreased hematocrit concentration was seen in 18.3%. Most affected cattle had reduced mean corpuscular volume (43.7%), mean corpuscular hemoglobin (14.3%), and mean corpuscular hemoglobin concentration (11.5%). All cattle with abnormal platelets count had thrombocytopenia. Hyperfibrinogenemia, hyperproteinemia, and hyperalbuminemia were found in 69%, 59.6%, and 37.2% of affected cattle, respectively. Decreased creatinine concentration was seen in 65.8%. Hyperkalemia and hyperchloremia was found in 9.6% and 10.4% of the affected cattle, respectively. Conclusions: LSD appears to be associated with inflammatory leukogram, anemia, thrombocytopenia, hyperfibrinogenemia, hyperproteinemia, decreased creatinine concentration, hyperchloramia, and hyperkalemia. These are likely due to the associated severe inflammatory process and disease complications such as anorexia and reduced muscle mass. This is the first study that documents hematological and serum biochemical findings associated with LSD infection. Understanding the blood profile picture may give further insight to the pathogenesis of the disease and help in treatment of individual cattle.

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Is erythrocyte size a strategy to avoid hypoxia in Wiegmann’s Torquate Lizards (Sceloporus torquatus)? Field evidence

Canadian Journal of Zoology ◽

10.1139/cjz-2014-0265 ◽

2015 ◽

Vol 93 (5) ◽

pp. 377-382 ◽

Cited By ~ 11

Author(s):

Juan C. González-Morales ◽

Erendira Quintana ◽

Hector Díaz-Albiter ◽

Palestina Guevara-Fiore ◽

Victor Fajardo

Keyword(s):

Physiological Response ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Mean Corpuscular Hemoglobin Concentration ◽

Corpuscular Hemoglobin ◽

Serum Electrolytes ◽

Blood Samples ◽

Field Evidence ◽

Different Altitudes

This study examined changes in certain hematological parameters in a reptilian model naturally exposed to altitude-associated hypoxia. Four populations of the Mexican lizard Sceloporus torquatus Wiegmann, 1828 (Wiegmann’s Torquate Lizard) from different altitudes were sampled to evaluate erythrocyte count (Erc), hematocrit (Hct), mean corpuscular hemoglobin concentration (MCHC), and erythrocyte size (Ers). Blood was also assayed to determine hemoglobin ([Hb]), glucose, lactate, and electrolyte concentrations. Erc was performed using a Neubauer hemocytometer. Hct was calculated as percentage of packed cell volume by centrifuging blood samples. [Hb] was determined using a Bausch and Lomb Spectronic colorimeter. MCHC was calculated with the formula 100 × [Hb]/Hct. Ers was calculated from blood smear microphotographs analyzed with the Sigma Scan Pro software. Values of serum electrolytes (sodium (Na+), potassium (K+), and calcium (Ca2+)), pH, glucose, and lactate from blood samples were obtained through a blood electrolyte analyzer. Highland populations of S. torquatus exhibited a significant increase in Erc, Hct, Ers, and [Hb]. In contrast, MCHC showed no correlation with altitude. Additionally, significant differences in lactate, Na+, K+, and Ca2+ were observed in highland populations. In general, we found that most hematological parameters were significantly different among lizard populations from different altitudes. This is the first study to report changes in Ers in relation to altitude, which could be a physiological response to hypoxia.

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Cogongrass (Imperata cylindrical L.) Roots Ethanol Extract to Improve Hematological Profile in Carbon Tetrachloride-Injection Mice Model

Global Medical & Health Communication (GMHC) ◽

10.29313/gmhc.v8i3.6605 ◽

2020 ◽

Vol 8 (3) ◽

Author(s):

Anisah Dahlan ◽

Fitria Hariati Ramdhani ◽

Neni Anggraeni ◽

Irma Melyani Puspitasari ◽

Mirasari Putri ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Mean Corpuscular Volume ◽

Hemoglobin Concentration ◽

Ethanol Extract ◽

Imperata Cylindrica ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Volume ◽

Mean Corpuscular Hemoglobin Concentration ◽

Corpuscular Hemoglobin ◽

Hematological Profile

Carbon tetrachloride (CCL4) is widely used in industry, toxic to the environment and humans, and most often used as a model of acute liver damage and liver fibrosis in experimental animals. Liver damage can deteriorate the hematological profile. The roots of cogongrass (Imperata cylindrica L.) have been used as traditional medicine due to its antioxidant activity. This study was conducted at the Faculty of Medicine, Universitas Padjadjaran, from January to March 2019. The study aimed to investigate whether the cogongrass roots ethanol extract (CGRE) can ameliorate the disturbance in the hematological profile in acute CCl4-injected mice. CGRE in dose 150 and 200 mg/kgBW was given orally to mice for four weeks before intraperitoneal injection of CCl4 1 mL/kgBW in olive oil (1:1 v/v). After 48 hours, mice were sacrificed, and the whole blood was drawn for hematological analysis. As a result, mean corpuscular volume (MCV) was reduced in CCl4-induction mice treated with CGRE in dose 150 mg/kgBW (49.25±3.06 vs 43.38±2.13 fl, p<0.05). This condition was followed by the improved hematocrit (Hct) and mean corpuscular hemoglobin concentration (MCHC). Platelet and platelet crit (Pct) levels were tended to decrease in CCl4-induction mice treated with CGRE in dose 150 mg/kgBW. In conclusion, CGRE dose 150 mg/kg BW can improve MCV, Hct, MCHC, platelet, and Pct in CCl4-injection mice. The antioxidant level in CGRE might facilitate it. EKSTRAK ETANOL AKAR ALANG-ALANG (IMPERATA CYLINDRICAL L.) MEMPERBAIKI PROFIL HEMATOLOGI PADA MENCIT YANG DIINJEKSI CARBON TETRACHLORIDECarbon tetrachloride (CCL4) banyak digunakan pada industri, bersifat toksik bagi lingkungan dan manusia, serta sering digunakan pada hewan coba untuk kerusakan liver akut dan fibrosis. Kerusakan liver dapat menyebabkan gangguan profil hematologi. Akar alang-alang (Imperata cylindrica L.) telah digunakan sebagai obat tradisional karena memiliki aktivitas antioksidan. Penelitian ini dilakukan di Fakultas Kedokteran Universitas Padjadjaran pada bulan Januari hingga Maret 2019. Tujuan penelitian ini adalah meneliti apakah ekstrak etanol akar alang-alang dapat memperbaiki gangguan profil hematologi pada mencit yang diinjeksi CCL4 secara akut. Ekstrak etanol akar alang-alang (EEAA) dosis 150 dan 200 mm/kgBB diberikan per oral kepada mencit selama empat minggu sebelum injeksi intraperitoneal CCl4 1 mL/kgBB yang dilarutkan dalam minyak zaitun (1:1 v/v). Setelah 48 jam, mencit dikorbankan dan diambil darahnya untuk pemeriksaan hematologi. Sebagai hasil, mean corpuscular volume (MCV) menurun pada mencit yang diinduksi CCl4 dengan perlakuan EEAA 150 mg/kgBB (49,25±3,06vs 43,38±2,13 fl, p<0,05). Keadaan ini diikuti dengan perbaikan hematokrit (Hct) dan mean corpuscular hemoglobin concentration (MCHC). Kadar platelet dan platelet crit (Pct) cenderung menurun pada mencit yang diinduksi CCl4 dengan perlakuan EEAA 150 mg/kgBB. Sebagai simpulan, EEAA dosis 150 mg/kgBB dapat memperbaiki MCV, Hct, MCHC, platelet, dan Pct pada mencit yang diinjeksi CCL4. Kemungkinan difasilitasi oleh antioksidan pada EEAA.

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