Determination of Maximum Accumulated Oxygen Deficit Using Backward Extrapolation

2020 ◽  
Author(s):  
Vitor Luiz Andrade ◽  
Carlos Augusto Kalva-Filho ◽  
Nayan Xavier Ribeiro ◽  
Ronaldo Bucken Gobbi ◽  
Tarine Botta de Arruda ◽  
...  
Keyword(s):  
Anaerobic Capacity ◽  
Graphical Analysis ◽  
Oxygen Deficit ◽  
Extrapolation Technique ◽  
Coefficients Of Variation ◽  
Intraclass Correlations ◽  
Accumulated Oxygen Deficit ◽  
The Difference ◽  
Predicted Values

AbstractThis study aimed to compare the Maximum Accumulated Oxygen Deficit determined by the conventional method (MAODC) with that determined by the backward extrapolation technique (MAODEXTR) in runners. Fourteen runners underwent a maximal incremental test for determination of iVO2MAX, ten submaximal efforts (50–95% of iVO2MAX for 7 min). During the submaximal efforts oxygen consumption (VO2) values were obtained conventionally and through the backward extrapolation technique (~ 3 s after the end of each effort). A supramaximal effort (110% of iVO2MAX) (tLimC) and five supramaximal bouts (tLimEXTR) were performed. MAODC and MAODEXTR were determined from the difference between the VO2 accumulated during tLimC and tLimEXTR and the predicted values. The tLimC was lower than tLimEXTR (164.06±36.32 s, 200.23±63.78 s, p<0.05). No significant differences were found between absolute and relative MAODC and MAODEXTR values, however, low intraclass correlations (0.26 and 0.24), high typical errors (2.03 L and 24 mL∙kg−1) were observed, and coefficients of variation (46 and 48%), respectively. The graphical analysis of the differences showed agreement and correlation between the methods (r=0.86 and 0.85). Thus, it can be concluded that the MAODEXTR is not a valid method for estimating the anaerobic capacity of runners, moreover, unreliable.

Accumulated oxygen deficit increases with inclination of uphill running

1992 ◽  
Vol 73 (3) ◽  
pp. 1130-1134 ◽  
Author(s):  
H. L. Olesen
Keyword(s):  
Oxygen Uptake ◽  
Energy Demand ◽  
Anaerobic Metabolism ◽  
Anaerobic Capacity ◽  
Submaximal Exercise ◽  
Oxygen Deficit ◽  
Linear Extrapolation ◽  
Trained Subjects ◽  
Accumulated Oxygen Deficit ◽  
The Difference

This study examined whether accumulated oxygen deficit depends on treadmill grade during uphill running. Oxygen uptake was measured during steady-state submaximal running. By linear extrapolation at each grade, energy demand was estimated for short exhaustive runs. Oxygen deficit was the difference between this estimate and accumulated oxygen uptake. Six subjects ran at grades of 1, 15, and 20% (study I), and five males trained for anaerobic metabolism ran at 1, 10.5, and 15% (study II). Accumulated oxygen deficit was 40 +/- 11 (SD), 72 +/- 20, and 69 +/- 8 ml O2/kg, respectively (study I), and 57 +/- 8, 78 +/- 10, and 100 +/- 7 ml O2/kg (study II). The finding that accumulated oxygen deficit became larger with treadmill inclination could reflect involvement of an increasing muscle mass. However, variation in accumulated oxygen deficit was too large to make this possibility the only explanation. More likely at small treadmill inclinations energy demand for high-intensity running is underestimated by extrapolation from oxygen uptake during submaximal exercise. At high grades of uphill running, accumulated oxygen deficit reached a maximum that may reflect the subjects' anaerobic capacity for running. This hypothesis was substantiated by an enhanced accumulated oxygen deficit in the anaerobically trained subjects during 15%, but not during 1%, uphill running.

Maximal accumulated oxygen deficit in thoroughbred horses

1995 ◽  
Vol 78 (4) ◽  
pp. 1564-1568 ◽  
Author(s):  
M. D. Eaton ◽  
D. L. Evans ◽  
D. R. Hodgson ◽  
R. J. Rose
Keyword(s):  
Aerobic Capacity ◽  
Anaerobic Capacity ◽  
Oxygen Deficit ◽  
Open Flow ◽  
Thoroughbred Horses ◽  
Accumulated Oxygen Deficit ◽  
The Mean ◽  
The Difference ◽  
Aerobic And Anaerobic ◽  
O2 Deficit

Thoroughbred horses have a high aerobic capacity, approximately twice that of elite human athletes. Whereas the aerobic capacity of horses can be accurately measured, there have been no measurements of anaerobic capacity. The aim of this study was to determine whether maximal accumulated O2 deficit (MAOD) could be measured in horses and used as an estimate of anaerobic capacity, as in human athletes. Six fit Thoroughbred horses were used with the exercise protocol utilizing a treadmill set at a 10% incline. O2 uptake VO2 was measured via an open-flow system for seven submaximal speeds (3–9 m/s), and maximal VO2 (135 +/- 3 ml.kg-1.min-1) was determined. The horses performed three tests at 105 and 125% and six tests at 115% of maximal VO2. The MAOD test was performed with the treadmill accelerated rapidly from 1.5 m/s (mean acceleration time 8 s) to the calculated speed (11–14 m/s). VO2 was measured every 10 or 15 s, and the test ended when the horse no longer kept pace with the treadmill. The mean run times were 165, 98, and 57 s for intensities of 105, 115, and 125% maximal VO2. The mean MAOD values were 31 +/- 2, 30 +/- 1, and 32 +/- 2 (SE) ml O2 eq/kg for the three intensities (P > 0.05). The proportion of energy derived from aerobic and anaerobic sources was calculated from the difference between calculated O2 demand and the VO2 curve. There was no correlation between MAOD and maximal VO2.(ABSTRACT TRUNCATED AT 250 WORDS)

An Examination of the Anaerobic Capacity of Children Using Maximal Accumulated Oxygen Deficit

1993 ◽  
Vol 5 (1) ◽  
pp. 60-71 ◽  
Author(s):  
John S. Carlson ◽  
Geraldine A. Naughton
Keyword(s):  
Steady State ◽  
Oxygen Uptake ◽  
Anaerobic Capacity ◽  
Peak Oxygen Uptake ◽  
Cycle Ergometer ◽  
Oxygen Deficit ◽  
Healthy Children ◽  
Downward Shift ◽  
Intraclass Correlations ◽  
Accumulated Oxygen Deficit

The purpose of this study was to determine the anaerobic capacity of children using the maximal accumulated oxygen deficit technique (AOD). Eighteen healthy children (9 boys, 9 girls) with a mean age of 10.6 years volunteered as subjects. Peak oxygen uptake and submaximal steady-state oxygen uptake tests were conducted against progressive constant work rates on a Cybex cycle ergometer. Supramaximal work rates were predicted from the linear regression of submaximal steady-state work rates and oxygen uptakes to equal 110, 130, and 150% of peak oxygen uptake. Results indicated a significant interaction in the responses of both sexes when the accumulated oxygen deficit data were expressed in both absolute and relative terms. The profile of accumulated oxygen deficits across the three intensities indicated a downward shift in the girls responses between the 110 and 150% supramaximal tests. This trend was not evident in the boys’ responses. Intraclass correlations conducted on test-retest data indicated that compared to the boys, the reliability of the girls in the accumulated oxygen deficits in liters and ml·kg−1 was poorer.

Pyridine Extraction Method for Determination of Bacitracin in Feeds: Collaborative Evaluation

1979 ◽  
Vol 62 (3) ◽  
pp. 662-670
Author(s):  
Hussein S Ragheb
Keyword(s):  
High Temperature ◽  
Extraction Method ◽  
Main Difficulty ◽  
Coefficients Of Variation ◽  
Significant Difference ◽  
The Difference ◽  
Zinc Bacitracin ◽  
Collaborative Evaluation

Abstract Seventeen laboratories evaluated the pyridine extraction method and neomycin-sensitized agar for the determination of zinc and MD bacitracin in swine and broiler rations at 10 and 100 g/ton. The method was also applied to the analysis of 2 premixes labeled 50 g/lb (MD bacitracin) and 40 g/lb (zinc bacitracin). Bacitracin activity was determined on each of 2 days with 2 dilutions on each day. No significant difference was found between dilutions within a day or between days for each sample. The type of bacitracin or type of feed did not significantly affect results. The difference in results between MD and zinc bacitracin in premixes approached significance. The large coefficients of variation for premixes (ca 13%) and complete feeds (ca 15–30%) indicate operational problems. The main difficulty was evaporation of pyridine. Some laboratories were not able to evaporate it completely, whereas others lost bacitracin activity, probably due to high temperature of drying. The pyridine extraction method as in 42.200 and 42.204 should be discontinued.

Differential assay of zidovudine and its glucuronide metabolite in serum and urine with a radioimmunoassay kit

1990 ◽  
Vol 36 (6) ◽  
pp. 897-900 ◽  
Author(s):  
S M Tadepalli ◽  
L Puckett ◽  
S Jeal ◽  
L Kanics ◽  
R P Quinn
Keyword(s):  
Inhibitory Concentration ◽  
Serum Samples ◽  
Lower Detection Limit ◽  
Coefficients Of Variation ◽  
Lower Detection ◽  
Before And After ◽  
The Difference ◽  
Glucuronide Metabolite ◽  
Serum And Urine

Abstract We developed an ancillary procedure for the ZDV-Trac RIA (Incstar) to allow simultaneous determination of both zidovudine (3'-azido-3'-deoxythymidine, ZDV, AZT, Retrovir) and its metabolite, the glucuronide of ZDV (3'-azido-3'-deoxy-5'-O-beta-D-glucopyranuronosylthymidine, ZDVG, GAZT), in human serum and urine. Using the ZDV-Trac RIA, we measured ZDV concentrations before and after ZDVG in samples was hydrolyzed to ZDV by beta-glucuronidase (EC 3.2.1.31); ZDVG concentration was calculated as the difference between the two results. This method enables rapid evaluation of a large number of samples with a total turn-around time of 6 h. The lower detection limit of the RIA was 0.27 micrograms/L; the measurements varied linearly with ZDV concentrations from 0.27 to 217 micrograms/L, with the 50% inhibitory concentration being approximately 10 micrograms/L. Analytical recoveries of inhouse serum and urine controls for both ZDV and ZDVG exceeded 90%. Coefficients of variation (CVs) of serum controls were less than 6% for ZDV and less than 11% for ZDVG; for urine controls, CVs for both ZDV and ZDVG were less than 6%. Results for ZDVG concentrations obtained by HPLC and by the ZDV-Trac RIA system compared well: r = 0.978, slope 1.0, for serum samples, and r = 0.993, slope 1.09, for urine samples.

Anaerobic Capacity: A Maximal Anaerobic Running Test Versus the Maximal Accumulated Oxygen Deficit

1996 ◽  
Vol 21 (1) ◽  
pp. 35-47 ◽  
Author(s):  
Neil S. Maxwell ◽  
Myra A. Nimmo
Keyword(s):  
Blood Lactate ◽  
Intermittent Exercise ◽  
Anaerobic Capacity ◽  
Capillary Blood ◽  
Treadmill Running ◽  
Oxygen Deficit ◽  
Blood Samples ◽  
Significant Difference ◽  
Accumulated Oxygen Deficit ◽  
Exercise Treadmill

The present investigation evaluates a maximal anaerobic running test (MART) against the maximal accumulated oxygen deficit (MAOD) for the determination of anaerobic capacity. Essentially, this involved comparing 18 male students performing two randomly assigned supramaximal runs to exhaustion on separate days. Post warm-up and 1, 3, and 6 min postexercise capillary blood samples were taken during both tests for plasma blood lactate (BLa) determination. In the MART only, blood ammonia (BNH3) concentration was measured, while capillary blood samples were additionally taken after every second sprint for BLa determination. Anaerobic capacity, measured as oxygen equivalents in the MART protocol, averaged 112.2 ± 5.2 ml∙kg−1∙min−1. Oxygen deficit, representing the anaerobic capacity in the MAOD test, was an average of 74.6 ± 7.3 ml∙kg−1. There was a significant correlation between the MART and MAOD (r =.83, p <.001). BLa values obtained over time in the two tests showed no significant difference, nor was there any difference in the peak BLa recorded. Peak BNH3 concentration recorded was significantly increased from resting levels at exhaustion during the MART. Key words: supramaximal intermittent exercise, treadmill running performance, blood lactate, ammonia

Caffeine Improved Time to Exhaustion But Did Not Change Alternative Maximal Accumulated Oxygen Deficit Estimated During a Single Supramaximal Running Bout

2016 ◽  
Vol 26 (6) ◽  
pp. 549-557 ◽  
Author(s):  
Rodrigo De Araujo Bonetti De Poli ◽  
Willian Eiji Miyagi ◽  
Fabio Yuzo Nakamura ◽  
Alessandro Moura Zagatto
Keyword(s):  
Anaerobic Capacity ◽  
Oxygen Deficit ◽  
Time To Exhaustion ◽  
Placebo Condition ◽  
Double Blind ◽  
Caffeine Ingestion ◽  
Total Body Mass ◽  
Accumulated Oxygen Deficit ◽  
Negative Effect ◽  
Positive Effect

The aim of the current study was to investigate the effects of acute caffeine supplementation on anaerobic capacity determined by the alternative maximal accumulated oxygen deficit (MAODALT) in running effort. Eighteen recreational male runners [29 ± 7years; total body mass 72.1 ± 5.8 kg; height 176.0 ± 5.4cm; maximal oxygen uptake (VO2max) 55.8 ± 4.2 ml·kg-1 ·min-1] underwent a graded exercise test. Caffeine (6 mg·kg-1) or a placebo were administered 1 hr before the supramaximal effort at 115% of the intensity associated with VO2max in a double-blind, randomized cross-over study, for MAODALT assessment. The time to exhaustion under caffeine condition (130.2 ± 24.5s) was 11.3% higher (p = .01) than placebo condition (118.8 ± 24.9 s) and the qualitative inference for substantial changes showed a very likely positive effect (93%). The net participation of the oxidative phosphorylation pathway was significantly higher in the caffeine condition (p = .02) and showed a likely positive effect (90%) of 15.3% with caffeine supplementation. The time constant of abrupt decay of excess postexercise oxygen consumption (τ1) was significantly different between caffeine and placebo conditions (p = .03) and showed a likely negative effect (90%), decreasing -8.0% with caffeine supplementation. The oxygen equivalents estimated from the glycolytic and phosphagen metabolic pathways showed a possibly positive effect (68%) and possibly negative effect (78%) in the qualitative inference with caffeine ingestion, respectively. However, the MAODALT did not differ under the caffeine or placebo conditions (p = .68). Therefore, we can conclude that acute caffeine ingestion does not modify the MAODALT, reinforcing the robustness of this method. However, caffeine ingestion can alter the glycolytic and phosphagen metabolic pathway contributions to MAODALT.

Assessment of Child-Specific Aerobic Fitness and Anaerobic Capacity by the Use of the Power-Time Relationships Constants

2010 ◽  
Vol 22 (3) ◽  
pp. 454-466 ◽  
Author(s):  
Erwan Leclair ◽  
Benoit Borel ◽  
Delphine Thevenet ◽  
Georges Baquet ◽  
Patrick Mucci ◽  
...  
Keyword(s):  
Critical Power ◽  
Aerobic Power ◽  
Anaerobic Capacity ◽  
Work Capacity ◽  
Constant Load ◽  
Evaluation Methods ◽  
Oxygen Deficit ◽  
Anaerobic Work Capacity ◽  
Accumulated Oxygen Deficit ◽  
Anaerobic Work

This study first aimed to compare critical power (CP) and anaerobic work capacity (AWC), to laboratory standard evaluation methods such as maximal oxygen uptake (V̇O2max) and maximal accumulated oxygen deficit (MAOD). Secondly, this study compared child and adult CP and AWC values. Subjects performed a maximal graded test to determine V̇O2max and maximal aerobic power (MAP); and four constant load exercises. In children, CP (W.kg−1) was related to V̇O2max (ml.kg−1.min−1; r = .68; p = .004). AWC (J.kg−1) in children was related to MAOD (r = .58; p = .018). Children presented lower AWC (J.kg−1; p = .001) than adults, but similar CP (%MAP) values. CP (%MAP and W.kg−1) and AWC (J.kg−1) were significantly related to laboratory standard evaluation methods but low correlation indicated that they cannot be used interchangeably. CP (%MAP) was similar in children and adults, but AWC (J.kg−1) was significantly lower in children. These conclusions support existing knowledge related to child-adults characteristics.

Maximal Accumulated Oxygen Deficit of Resistance-Trained Men

1996 ◽  
Vol 21 (5) ◽  
pp. 391-402 ◽  
Author(s):  
Francis X. Pizza ◽  
Thomas A. Naglieri ◽  
Robert W. Holtz ◽  
Joel B. Mitchell ◽  
Raymond D. Starling ◽  
...  
Keyword(s):  
Key Words ◽  
Muscle Mass ◽  
Significant Positive Correlation ◽  
Anaerobic Capacity ◽  
Constant Load ◽  
Oxygen Deficit ◽  
Secondary Purpose ◽  
Accumulated Oxygen Deficit ◽  
Leg Muscle ◽  
The Relationship

The primary purpose of the study was to compare maximal accumulated oxygen deficit (MAOD) in resistance-trained (RT), endurance-trained (ET), and untrained men (UT). A secondary purpose was to determine the influence of leg muscle mass (MM) on MAOD by examining the relationship between MM and MAOD and by comparing MAOD expressed relative to MM between the groups. MAOD was determined during 2-4 min of constant-load fatiguing cycling. MM, estimated via anthropometric measurements, was higher (p <.05) for RT (mean ± SE; 25.5 ± 3.4 kg) compared to ET (20.3 ± 3.5) and UT (21.6 ± 3.4). MAOD in liters O2 eq was larger in RT (4.75 ± 0.3) compared to UT (3.07 ± 0.3) and ET (3.75 ± 0.3). A significant positive correlation was observed between MAOD (LO2 eq) and MM (kg) for RT only (RT, r =.85; ET, r =.55; UT, r =.20). Based on the correlational and mean MM data, the higher MAOD (LO2 eq) in RT relative to ET and UT is predominantly the result of their larger leg muscle mass. Key words: exercise, anaerobic capacity, muscle mass

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