EVALUATION OF AN AUTOMATED COAGULATION ANALYZER

1987 ◽  
Author(s):  
M Johnston ◽  
M Andrew
Keyword(s):  
Thrombolytic Therapy ◽  
Light Source ◽  
Therapy Group ◽  
Screening Tests ◽  
Poor Correlation ◽  
Mean Values ◽  
Halogen Light ◽  
Halogen Light Source ◽  
Scientific Group ◽  
Time Required

The ACL (IL-Automated Coagulation Laboratory from the Fisher Scientific Group) is the first microcentrifugal analyzer incorporating 2 reading channels, a coagulometric channel consisting of a laser light source and a chromogenic channel consisting of a halogen light source. We have evaluated the instrument for precision and accuracy using different reagents for both clotting and the chromogenic assays. Replicate samples were run in both the PT and APTT modes using 4 different reagents. The reagent with the least particles had the greatest precision. The mean values for APTTs and PTs using a particle free reagent were 68.7 ± .83“ for the APTT (C.V. 1.2%), and 30.2 ± .33” (C.V. 1.1%) for the PT. A fibrinogen assay measured on the ACL (delta light scatter of the prothrombin time) was compared to the Clause fibrinogen assay in three population groups: the adult, the newborn and patients receiving thrombolytic therapy. The correlation for the adult and newborn was good with r values of .911 (n = 51) and .96 (n = 36) respectively. The thrombolytic therapy group had poor correlation r = .32. Antithrombin III (ATIII) and a2 antiplasmin (AP) assays were measured on the ACL using IIs chromogenic method. These were compared to an ATIII chromogenic method (Kahle) using a Gilford SBA 300 analyzer and α2 AP assay using a Protopath (Dade). The correlations were ATIII, r=.95 and α2 AP, r=.82. The plasminogen method of Friberger was adapted to the ACL giving us comparable results to those read off the Gilford SBA 300 (r=.93). With the introduction of the ACL we have been able to: 1) reduce the technical time required for assays by one half; 2) reduce reagent costs by one half to three-quarters; 3) reduce the amount of plasma required for screening tests by half the volume, which has greatly facilitated neonatal coagulation testing

Comparison of the effects of operating microscopes with light emitting diode and halogen light source on the eye: a rabbit study

2021 ◽  
pp. 1-7
Author(s):  
Bahri Aydın ◽  
Armagan Ozgur ◽  
Huseyin Baran Ozdemir ◽  
Pınar Uyar Gocun ◽  
Mehmet Arda Inan ◽  
...  
Keyword(s):  
Light Source ◽  
Light Emitting Diode ◽  
Light Emitting ◽  
Halogen Light ◽  
Halogen Light Source

scholarly journals Effect of halogene light source on marginal microleakage of resin composite restorations

2010 ◽  
Vol 57 (1) ◽  
pp. 14-20
Author(s):  
Natasa Gajic ◽  
Aleksandra Djeri ◽  
Zeljka Kojic ◽  
Natasa Trtic
Keyword(s):  
Composite Materials ◽  
Light Source ◽  
Resin Composite ◽  
Human Teeth ◽  
Class V ◽  
Composite Restorations ◽  
Halogen Light ◽  
Significant Difference ◽  
Halogen Light Source ◽  
Control Light

Introduction. Although the technology of production resin composite materials has been improved in the past years, polymerization shrinkage and microleakage still remain the main problem of the resin composite restorations. The most important issues are polymerization control, light source choice, quality and polymerization technique. The aim of this study was to evaluate the effect of the halogen light source on the marginal microleakage of the resin composite restorations class V. Material and Methods. The study included 40 extracted human teeth (20 intact and 20 with caries lesion). Class V cavity was prepared in each tooth. The light curing unit used in this study had irradiance 540 mW/cm2. Cavities were restored with the two resin composite materials, Filtek Supreme (3M ESPE) and Luksogal - Galenika. Microleakage was determined using colored solution of 50% silver-nitrate. Results were obtained using stereomicroscope with 6 times magnification. Color penetration was evaluated on the occlusal and gingival wall. Results. Linear color penetration was observed in all cavities restored using Luksogal. There was significant difference (p<0.01) in color penetration between the occlusal (20.30 ?m) and gingival wall (34.00 ?m). Color penetration was shown in all cavities prepared on intact and caries teeth and restored using Filtek Supreme. Also, color penetration on the occlusal wall (13.80 ?m) was significantly lower (p<0.01) than the penetration on the gingival wall (33.00 ?m). Conclusion. All the tested cavities showed marginal leakage, regardless the tooth or material used. Greater microleakage was noticed when Luksogal was used.

scholarly journals NanoLuc Bioluminescence-Driven Photodynamic Activation of Cholecystokinin 1 Receptor with Genetically-Encoded Protein Photosensitizer MiniSOG

2020 ◽  
Vol 21 (11) ◽  
pp. 3763 ◽  
Author(s):  
Yuan Li ◽  
Zong Jie Cui
Keyword(s):  
Singlet Oxygen ◽  
Light Source ◽  
Light Emitting Diode ◽  
Calcium Oscillations ◽  
Entry Site ◽  
Photodynamic Action ◽  
Halogen Light ◽  
Halogen Light Source ◽  
External Light Source

In contrast to reversible activation by agonist, cholecystokinin 1 receptor (CCK1R) is permanently activated by singlet oxygen generated in photodynamic action, with sulphonated aluminium phthalocyanine or genetically encoded mini singlet oxygen generator (miniSOG) as photosensitizer. In these works, a halogen light source was used to power photodynamic action. For possible in vivo application of photodynamic CCK1R physiology, bearing a cumbersome light-delivery device connected to an external light source by experimental animals might interfere with their behavior. Therefore, in the present work, the possibility of bioluminescence-driven miniSOG photodynamic CCK1R activation was examined, as monitored by Fura-2 calcium imaging. In parallel experiments, it was found that, after plasma membrane (PM)-localized expression of miniSOGPM in AR4-2J cells, light irradiation with blue light-emitting diode (LED) (450 nm, 85 mW·cm−2, 1.5 min) induced persistent calcium oscillations that were blocked by CCK1R antagonist devazepide 2 nM. NanoLuc was expressed bicistronically with miniSOGPM via an internal ribosome entry site (IRES) sequence (pminiSOGPM-IRES-NanoLuc). The resultant miniSOGPM-IRES-NanoLuc-AR4-2J cells were found to generate strong bioluminescence upon addition of NanoLuc substrate coelenterazine. Strikingly, coelenterazine 5 microM was found to trigger long-lasting calcium oscillations (a hallmark for permanent CCK1R activation) in perifused miniSOGPM-IRES-NanoLuc-AR4-2J cells. These data indicate that NanoLuc bioluminescence can drive miniSOGPM photodynamic CCK1R activation, laying the foundation for its future in vivo applications.

Evaluation of biochemical changes in dental tissues after different office bleaching methods

2018 ◽  
Vol 38 (4) ◽  
pp. 389-397 ◽  
Author(s):  
ES Karaarslan ◽  
ZC Özmen ◽  
F Aytac ◽  
AA Bicakci ◽  
M Buldur ◽  
...  
Keyword(s):  
Biochemical Changes ◽  
Pulp Tissue ◽  
Dental Tissues ◽  
Group 4 ◽  
Halogen Light ◽  
Halogen Light Source ◽  
Proteolytic Activities ◽  
Group 2 ◽  
Increasing Demand ◽  
Laser Bleaching

The color of the teeth is an important topic for many people and can be influenced by intrinsic and extrinsic stains. There is an increasing demand for whitening of the teeth year by year. The most popular way of whitening is “bleaching,” which is the result of the breakdown of pigments located in the enamel and/or the dentin, caused by reactive oxygen species (ROS) released from bleaching agents. These bleaching agents could increase matrix metalloproteinase (MMP)-mediated collagen degradation in dentin. The aim of this study was to compare biochemical changes and oxidative stress levels of the human premolar dentin–pulp complex after three different bleaching methods containing hydrogen peroxide (H2O2) bleaching agents. Individuals, whose first four premolars were extracted for orthodontic purposes, included into the study. Group 1—Laser: bleaching gel containing 46% H2O2 (LaserWhite20 whitening gel, Biolase Technology Inc., San Clemente, CA, USA) and a diode laser activation (Ezlase 940 nm system). Group 2: 35% H2O2 containing whitening gel (Whiteness HP Maxx, FGM) and halogen light source activation (Optilux 501, Kerr, Orange, CA, USA). Group 3: 35% H2O2 containing whitening gel (Whiteness HP Maxx, FGM). Group 4—Control: No whitening treatment. According to the test results, there were no significant differences among groups in the values of cathepsin B and MMP proteolytic activities ( p > 0.05). The total ROS values released from the dentin tissue were higher than those obtained from the pulp tissue ( p < 0.05). There were significant differences among the bleaching groups in the ROS values released from the dentin tissue.

Comparison of Composite Curing Parameters: Effects of Light Source and Curing Mode on Conversion, Temperature Rise and Polymerization Shrinkage

10.2341/05-15 ◽  
2006 ◽  
Vol 31 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Z. Tarle ◽  
A. Knezevic ◽  
N. Demoli ◽  
A. Meniga ◽  
J. Sutalo ◽  
...  
Keyword(s):  
Light Source ◽  
Temperature Rise ◽  
Thermal Injury ◽  
Resin Composite ◽  
Marginal Adaptation ◽  
Low Intensity ◽  
Halogen Light ◽  
Lower Temperature ◽  
Final Degree ◽  
Conversion Temperature

Clinical Relevance The use of a low intensity light source for photopolymerization based on LED technology provides equivalent final degree conversion with possible flow of the resin composite, similar to when QTH technology is used. At the same time, the lower temperature rise in the sample and the more favorable development of shrinkage kinetics compared to the higher intensities of halogen light may aid in maintaining marginal adaptation while avoiding possible thermal injury.

Sign in / Sign up

Export Citation Format

Share Document