Comparison of the effects of operating microscopes with light emitting diode and halogen light source on the eye: a rabbit study

Author(s):  
Bahri Aydın ◽  
Armagan Ozgur ◽  
Huseyin Baran Ozdemir ◽  
Pınar Uyar Gocun ◽  
Mehmet Arda Inan ◽  
...  
2009 ◽  
Vol 79 (1) ◽  
pp. 144-149 ◽  
Author(s):  
Mustafa Ulker ◽  
Tancan Uysal ◽  
Sabri Ilhan Ramoglu ◽  
Huseyin Ertas

Abstract Objective: To compare the microleakage of the enamel-adhesive-bracket complex at the occlusal and gingival margins of brackets bonded with high-intensity light curing lights and conventional halogen lights. Materials and Methods: Forty-five freshly extracted human maxillary premolar teeth were randomly separated into three groups of 15 teeth each. Stainless steel brackets were bonded in all groups according to the manufacturer's recommendations. Specimens (15 per group) were cured for 40 seconds with a conventional halogen light, 20 seconds with light-emitting diode (LED), and 6 seconds with plasma arc curing light (PAC). After curing, the specimens were further sealed with nail varnish, stained with 0.5% basic-fuchsine for 24 hours, sectioned and examined under a stereomicroscope, and scored for microleakage for the enamel-adhesive and bracket-adhesive interfaces from both the occlusal and gingival margins. Statistical analyses were performed using Kruskal-Wallis and Mann-Whitney U-tests with a Bonferroni correction. Results: The type of light curing unit did not significantly affect the amount of microleakage at the gingival or occlusal margins of investigated interfaces (P >.05). The gingival sides in the LED and PAC groups exhibited higher microleakage scores compared with those observed on occlusal sides for the enamel-adhesive and adhesive-bracket interfaces. The halogen light source showed similar microleakage at the gingival and occlusal sides between both adhesive interfaces. Conclusions: High-intensity curing units did not cause more microleakage than conventional halogen lights. This supports the use of all these curing units in routine orthodontic practice.


2016 ◽  
Vol 78 (11) ◽  
Author(s):  
Khairunnisa Mohd Yusof ◽  
Suhaila Isaak ◽  
Nurfatihah Che Abd Rashid ◽  
Nor Hafizah Ngajikin

Soil is a medium for plant roots to grow, absorb water and necessary solutes for growth. Soil macronutrient testing is helpful for determining the nutrients content in soil before applying fertilizer for quality and process controls of agricultural produce and soil fertility. Spectroscopy is an emerging technology which is rapid and simple has been widely used in agricultural and food analysis processes. The capability of spectroscopy to characterize material from the transmission or absorbance has been used in this paper to measure nitrogen (N), phosphorus (P) and potassium (K) content in non-agriculture soil. The paper details preliminary characterization of soil spectroscopy with a Deuterium-Halogen light source and Ocean Optic spectrometer to measure the absorbance level of the macronutrients. The extracted nutrients were mixed with the colour reagent and specific colored solution was developed. Two soil samples have been employed for the experimental characterization, which are mud flood and kaolin. The result shows that high absorbance level of N at 450 nm in wavelength, P at 750 nm for both samples.  The absorbance level of K was measured high at 500nm for mud flood and 450nm for kaolin. In addition, the tested macronutrients give similar wavelength of peak absorbance level at 970 nm for both samples. For future works, the optical measurements will be implemented using visible and near infrared LED and the photodetector in order to replace the spectrometer usage for soil spectroscopy. This would lead to achieve the primary objective of this research in developing a simple and low cost spectroscopy uses light-emitting diode (LED).


2020 ◽  
Vol 21 (11) ◽  
pp. 3763 ◽  
Author(s):  
Yuan Li ◽  
Zong Jie Cui

In contrast to reversible activation by agonist, cholecystokinin 1 receptor (CCK1R) is permanently activated by singlet oxygen generated in photodynamic action, with sulphonated aluminium phthalocyanine or genetically encoded mini singlet oxygen generator (miniSOG) as photosensitizer. In these works, a halogen light source was used to power photodynamic action. For possible in vivo application of photodynamic CCK1R physiology, bearing a cumbersome light-delivery device connected to an external light source by experimental animals might interfere with their behavior. Therefore, in the present work, the possibility of bioluminescence-driven miniSOG photodynamic CCK1R activation was examined, as monitored by Fura-2 calcium imaging. In parallel experiments, it was found that, after plasma membrane (PM)-localized expression of miniSOGPM in AR4-2J cells, light irradiation with blue light-emitting diode (LED) (450 nm, 85 mW·cm−2, 1.5 min) induced persistent calcium oscillations that were blocked by CCK1R antagonist devazepide 2 nM. NanoLuc was expressed bicistronically with miniSOGPM via an internal ribosome entry site (IRES) sequence (pminiSOGPM-IRES-NanoLuc). The resultant miniSOGPM-IRES-NanoLuc-AR4-2J cells were found to generate strong bioluminescence upon addition of NanoLuc substrate coelenterazine. Strikingly, coelenterazine 5 microM was found to trigger long-lasting calcium oscillations (a hallmark for permanent CCK1R activation) in perifused miniSOGPM-IRES-NanoLuc-AR4-2J cells. These data indicate that NanoLuc bioluminescence can drive miniSOGPM photodynamic CCK1R activation, laying the foundation for its future in vivo applications.


2021 ◽  
Vol 13 (9) ◽  
pp. 4852
Author(s):  
Jack Ngarambe ◽  
Inhan Kim ◽  
Geun Young Yun

Spectral power distribution (SPD) is an essential element that has considerable implications on circadian energy and the perception of lit environments. The present study assessed the potential influences of SPD on energy consumption (i.e., considering circadian energy), visual comfort, work performance and mood. Two lighting conditions based on light-emitting diode (LED) and organic light-emitting diode (OLED) were used as proxies for SPDs of different spectral content: dominant peak wavelength of 455 nm (LED) and 618 nm (OLED). Using measured photometric values, the circadian light (CL), melatonin suppression (MS), and circadian efficacy (CE) of the two lighting sources were estimated via a circadian-phototransduction model and compared. Additionally, twenty-six participants were asked to evaluate the said lit environments subjectively in terms of visual comfort and self-reported work performance. Regarding circadian lighting and the associated energy implications, the LED light source induced higher biological actions with relatively less energy than the OLED light source. For visual comfort, OLED lighting-based conditions were preferred to LED lighting-based conditions, while the opposite was true when considering work performance and mood. The current study adds to the on-going debate regarding human-centric lighting, particularly considering the role of SPD in energy-efficient and circadian lighting practices.


2018 ◽  
Vol 4 (11) ◽  
pp. 133
Author(s):  
HyungTae Kim ◽  
EungJoo Ha ◽  
KyungChan Jin ◽  
ByungWook Kim

A system for inspecting flat panel displays (FPDs) acquires scanning images using multiline charge-coupled device (CCD) cameras and industrial machine vision. Optical filters are currently installed in front of these inspection systems to obtain high-quality images. However, the combination of optical filters required is determined manually and by using empirical methods; this is referred to as passive color control. In this study, active color control is proposed for inspecting FPDs. This inspection scheme requires the scanning of images, which is achieved using a mixed color light source and a mixing algorithm. The light source utilizes high-power light emitting diodes (LEDs) of multiple colors and a communication port to dim their level. Mixed light illuminates an active-matrix organic light-emitting diode (AMOLED) panel after passing through a beam expander and after being shaped into a line beam. The image quality is then evaluated using the Tenenbaum gradient after intensity calibration of the scanning images. The dimming levels are determined using the simplex search method which maximizes the image quality. The color of the light was varied after every scan of an AMOLED panel, and the variation was iterated until the image quality approached a local maximization. The number of scans performed was less than 225, while the number of dimming level combinations was 20484. The proposed method can reduce manual tasks in setting-up inspection machines, and hence is useful for the inspection machines in FPD processes.


2010 ◽  
Vol 82 (7) ◽  
pp. 2734-2742 ◽  
Author(s):  
Erin L. Ratcliff ◽  
P. Alex Veneman ◽  
Adam Simmonds ◽  
Brian Zacher ◽  
Daniel Huebner ◽  
...  

Author(s):  
Anuradha Vitthal Wankhade ◽  
Sharad Basavraj Kamat ◽  
Santosh Irappa Hugar ◽  
Girish Shankar Nanjannawar ◽  
Sumit Balasaheb Vhate

Introduction: New generation composite resin materials have revolutionized the art of aesthetic dentistry. The clinical success is dependent on effective polymerisation and surface hardness which in turn are dependent on the performance of Light Curing Units (LCU). This study utilises surface hardness as a measure of degree of polymerisation of composite resins achieved by LCUs. Aim: To evaluate the difference in surface hardness of nanohybrid and microhybrid resin composites cured by light curing systems, Light Emitting Diode (LED) and Quartz Tungsten Halogen (QTH). Materials and Methods: In this invitro experimental study, two types of hybrid composites (Nanohybrid and Microhybrid) were tested for surface hardness by using two different light curing systems (LED and QTH). All the Nanohybrid and Microhybrid specimens were cured using LED and QTH LCUs, thus giving four combinations. A total of 60 specimens (6 mm diameter and 2 mm depth) were prepared using Teflon mould with 15 samples for each combination. Surface hardness was measured on upper and lower surface after 24 hours and hardness ratio was calculated. Data was analysed using independent t-test for intergroup comparison. Level of significance was kept at 5%. Results: Surface hardness of resin composites cured by LED LCU was greater than those cured by QTH LCU. Additionally, the hardness value was greater for the upper surface. Nanohybrids showed better surface hardness than Microhybrids for both the LCUs. Conclusion: Nanohybrid composite resins and LED system were found to be more effective in terms of surface hardness as compared to their counterparts.


2018 ◽  
Vol 29 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Philipp S Koelbl ◽  
Christian Lingenfelder ◽  
Christoph W Spraul ◽  
Juergen Kampmeier ◽  
Frank HJ Koch ◽  
...  

Purpose: Development of a new, fiber-free, single-use endo-illuminator for pars plana vitrectomy as a replacement for fiber-based systems with external light sources. The hand-guided intraocularly placed white micro light-emitting diode is evaluated for its illumination properties and potential photochemical and thermal hazards. Methods: A micro light-emitting diode was used to develop a single-use intraocular illumination system. The light-source-on-tip device was implemented in a prototype with 23G trocar compatible outer diameter of 0.6 mm. The experimental testing was performed on porcine eyes. All calculations of possible photochemical and thermal hazards during the application of the intraocular micro light-emitting diode were calculated according to DIN EN ISO 15007–2: 2014. Results: The endo-illuminator generated a homogeneous and bright illumination of the intraocular space. The color impression was physiologic and natural. Contrary to initial apprehension, the possible risk caused by inserting a light-emitting diode into the intraocular vitreous was much smaller when compared to conventional fiber-based illumination systems. The photochemical and thermal hazards allowed a continuous exposure time to the retina of at least 4.7 h. Conclusion: This first intraocular light source showed that a light-emitting diode can be introduced into the eye. The system can be built as single-use illumination system. This light-source-on-tip light-emitting diode–endo-illumination combines a chandelier wide-angle illumination with an adjustable endo-illuminator.


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