The Influence of Inorganic Salts on Plasma Antithrombin Activity

1963 ◽  
Vol 09 (02) ◽  
pp. 387-394 ◽  
Author(s):  
Frank C Monkhouse
Keyword(s):  
Ionic Strength ◽  
Alpha Tocopherol ◽  
Inorganic Salts ◽  
Distilled Water ◽  
Antithrombin Activity ◽  
Phosphate Ion ◽  
Factors Influencing ◽  
Increased Activity

SummaryA study has been made of factors influencing the activities of plasma antithrombin. Optimal antithrombin activity was obtained between pH 7.8 and 8.5. Dialysis against distilled water reduced the antithrombin activity to less than 30 per cent of normal. The antithrombin activity was restored by the addition, directly or by dialysis, of a number of salts. The increased activity in the presence of the salts seemed to be a function of the polyvalent anions rather than of the ionic strength of the medium. The antithrombin activity of alpha- tocopherol phosphate in vitro appears to be a function of the phosphate ion and not of the vitamin itself.

Factors influencing zoospore production by Phytophthora cinnamomi in axenic cultui

1980 ◽  
Vol 58 (19) ◽  
pp. 2117-2122 ◽  
Author(s):  
Calvin L. Schoulties ◽  
Kenneth F. Baker ◽  
Carol Sabersky-Lehmann
Keyword(s):  
Salt Solution ◽  
Phytophthora Cinnamomi ◽  
Mineral Salt ◽  
Distilled Water ◽  
Sporulation Medium ◽  
Uniform Size ◽  
Factors Influencing ◽  
Zoospore Release ◽  
Zoospore Production

Factors and procedures found to increase the quantity and consistency of axenic zoospore production in a selected isolate of Phytophthora cinnamomi were (i) the use of single-zoospore cultures of uniform size that were between 48 and 72 h old; (ii) thorough washing of mycelial mats at the time of sporangium induction to remove nutrients; (iii) agitation of the sporulation medium (mineral salt solution) 24 h after the initial induction; (iv) standardization of the volume of the sporulation medium; (v) adequate removal of the sporulation medium and replacement with distilled water before triggering zoospore release; and (vi) placement of colonies that had been induced to sporulate under light. The addition of a purified sporangium stimulatory substance to mycelial mats which had been induced to sporulate enabled the fungus to sporulate under conditions which normally suppressed sporulation in vitro. In the presence of this stimulatory substance, the fungus sporulated prolifically in darkness and with limited quantities of added nutrients. Other isolates of P. cinnamomi responded in a similar manner to many of these factors and procedures.

SEM Evaluation of Fluoride Pretreatment Effects on Acid-Etching of Caries-Like Enamel Lesions In Vitro

1981 ◽  
Vol 39 ◽  
pp. 474-475
Author(s):  
M. John Hicks
Keyword(s):  
Dental Enamel ◽  
Preventive Measure ◽  
Acid Etching ◽  
Distilled Water ◽  
Adhesive Resin ◽  
Fluoride Treatment ◽  
Treatment Groups ◽  
Pretreatment Effects ◽  
Preparation Techniques

Acid-etching of enamel surfaces has been performed routinely to bond adhesive resin materials to sound dental enamel as a caries-preventive measure. The effect of fluoride pretreatment on acid-etching of enamel has been reported to produce inconsistent and unsatisfactory etching patterns. The failure to obtain an adequate etch has been postulated to be due to fluoride precipitation products deposited on the enamel surface. The purpose of this study was to evaluate the effects of fluoride pretreatment on acid-etching of carieslike lesions of human dental enamel.Caries-like lesions of enamel were created in vitro on human molar and premolar teeth. The teeth were divided into two fluoride treatment groups. The specimens were exposed for 4 minutes to either a 2% Sodium Fluoride (NaF) solution or a 10% Stannous Fluoride (SnF2) solution. The specimens were then washed in deionized-distilled water. Each tooth was sectioned into four test regions. This was carried out to compare the effects of various time exposures (0 to 2 minutes) and differing concentrations (10 to 60% w/w) of phosphoric acid (H3PO4) on etching of caries-like lesions. Standard preparation techniques for SEM were performed on the specimens.

Factors Influencing in Vitro Rumen Cellulose Digestion

1956 ◽  
Vol 15 (4) ◽  
pp. 1180-1187 ◽  
Author(s):  
C. N. Huhtanen ◽  
R.F. Elliott
Keyword(s):  
Cellulose Digestion ◽  
Factors Influencing

Seed-borne fungi of cowpea [Vigna unguiculata (L.) Walp] and their possible control in vitro using locally available fungicides in Botswana.

2016 ◽  
Vol 5 (11) ◽  
pp. 5016 ◽  
Author(s):  
K. B. Khare* ◽  
Loeto D. ◽  
Wale K. ◽  
Salani M.
Keyword(s):  
Seed Germination ◽  
Continuous Light ◽  
Sodium Hypochlorite Solution ◽  
Distilled Water ◽  
Percentage Germination ◽  
Department Of Agriculture ◽  
Hypochlorite Solution ◽  
Cowpea Cultivars ◽  
Petri Plate

Seeds of three cowpea cultivars namely Black eye, ER 7 and Tswana obtained from the Department of Agriculture Research, Gaborone were tested for the presence of seed-borne fungi, and their possible control in vitro using locally available fungicides. Four hundred fifty seeds of each cultivar of cowpea were disinfected with 2% sodium hypochlorite solution for 10 min and washed three times with sterile distilled water before placing them in PDA plates (5 seeds/9 cm Petri plate), incubated at 22±2o C for 12 hour each under continuous light and dark. A total of eight fungi were detected from seeds of cowpea. These were Aspergillus flavus, A. niger, Cylindrocarpon sp., Fusarium equisiti, F. oxyaporum, Penicillium chyrosogenum, Rhizopus oligosporus and R. stolonifer. Rhizopus spp. were dominant fungi recovered from seeds, followed by Penicillium, Aspergillus, Fusarium and Cylindrocarpon. The fungi detected resulted in decay and rotting of seeds, and thereby reducing percentage germination of seeds (22%, 37% and 63 % seed germination in Black eye, ER7 and Tswana varieties of cowpea respectively). Out of four fungicides tested, benlate, captan, dithane M 45 and chlorothalanil. Dithane M45 effectively controlled seed-borne fungi, and enhanced seed germination to an average of 86% (93% germination with no fungi detected in Tswana variety) as compared to chlorothalonile (79%), benlate and captan (77%) and un-treated seeds (45%). The fungal incidence was reduced to 2.3%, 4.3%, 5.3% and 5.3% when seeds were treated with dithane M-45, chlorothalonil, benlate and captan respectively as compared to 62% in non-treated seeds.

scholarly journals Ibrexafungerp: A First-in-Class Oral Triterpenoid Glucan Synthase Inhibitor

2021 ◽  
Vol 7 (3) ◽  
pp. 163 ◽  
Author(s):  
Sabelle Jallow ◽  
Nelesh P. Govender
Keyword(s):  
Pathogenic Fungi ◽  
Candida Spp ◽  
Fungal Cell ◽  
Favourable Safety ◽  
Favourable Safety Profile ◽  
Synthase Inhibitor ◽  
Increased Activity ◽  
In Vitro Data

Ibrexafungerp (formerly SCY-078 or MK-3118) is a first-in-class triterpenoid antifungal or “fungerp” that inhibits biosynthesis of β-(1,3)-D-glucan in the fungal cell wall, a mechanism of action similar to that of echinocandins. Distinguishing characteristics of ibrexafungerp include oral bioavailability, a favourable safety profile, few drug–drug interactions, good tissue penetration, increased activity at low pH and activity against multi-drug resistant isolates including C. auris and C. glabrata. In vitro data has demonstrated broad and potent activity against Candida and Aspergillus species. Importantly, ibrexafungerp also has potent activity against azole-resistant isolates, including biofilm-forming Candida spp., and echinocandin-resistant isolates. It also has activity against the asci form of Pneumocystis spp., and other pathogenic fungi including some non-Candida yeasts and non-Aspergillus moulds. In vivo data have shown IBX to be effective for treatment of candidiasis and aspergillosis. Ibrexafungerp is effective for the treatment of acute vulvovaginal candidiasis in completed phase 3 clinical trials.

scholarly journals Comparison of the Cerumenolytic Activities of New and Currently Used Agents

2021 ◽  
pp. 014556132098606
Author(s):  
Nguyen Quynh Anh ◽  
Pawin Numthavaj ◽  
Thongchai Bhongmakapat
Keyword(s):  
Salicylic Acid ◽  
Sodium Bicarbonate ◽  
Potassium Hydroxide ◽  
Glycolic Acid ◽  
Statistical Significance ◽  
In Vitro Study ◽  
Distilled Water ◽  
Moderate Amount ◽  
All Solutions

Objectives: This study compared the cerumen dissolution activities of 7.5% sodium bicarbonate, 5% potassium hydroxide, 10% lactic acid, 3% salicylic acid, 10% glycolic acid, and distilled water. Methods: An in vitro study was conducted with 36 cerumen samples. The cerumenolytic activities of the 6 agents were assessed by recording the degree of cerumen disintegration using digital photography at 15 minutes, 30 minutes, 1 hour, 2 hours, and 12 hours. The undissolved cerumen that remained after 12 hours was removed from the solutions and weighed after drying. Results: Potassium hydroxide showed the fastest cerumenolytic activity, dissolving a moderate amount of cerumen at 30 minutes, while glycolic acid and salicylic acid caused no visible changes in the cerumen samples. Samples treated with potassium hydroxide and sodium bicarbonate exhibited higher degrees of disintegration compared to samples treated with distilled water (odds ratio and 95% CI: 273.237 [0.203-367 470.4] and 1.129 [0.002-850.341], respectively). The greatest reduction in cerumen weight was associated with the use of sodium bicarbonate; however, this result did not reach statistical significance. Conclusions: Among the solutions tested, 5% potassium hydroxide showed the fastest dissolution activity, yielding moderate disintegration within only 30 minutes. In terms of residual cerumen weight within 12 hours, all solutions exhibited equivalent effectiveness in the disintegration of cerumen.

scholarly journals Method for maintenance of coffee leaves in vitro for mass rearing of Leucoptera coffeellum (Guérin-Méneville) (Lepidoptera: Lyonetiidae)

2000 ◽  
Vol 29 (4) ◽  
pp. 849-854 ◽  
Author(s):  
Ronaldo Reis Jr. ◽  
Lima ◽  
Evaldo F. Vilela ◽  
Raimundo S. Barros
Keyword(s):  
Plant Growth ◽  
Mass Rearing ◽  
Developmental Cycle ◽  
Plant Tissues ◽  
Distilled Water ◽  
Complete Development ◽  
Detached Leaves ◽  
Leaf Survival ◽  
The Ideal

To accomplish systematic studies with coffee leafminer, it is necessary to establish a mass rearing system under artificial conditions. It is possible to rear this species, from egg to adult, under laboratory conditions, without using coffee seedlings but detached leaves maintained in vitro. Synthetic cytokinins are routinely used for maintenance of plant cell and plant tissues in vitro. Two plant growth regulators, benzyladenin and kinetin, in concentrations 10-6 and 10-7 M were used to mantain the leaves. Green leaves collected in the field were maintained in the solution to be tested. Distilled water served as control. The experiment lasted 30 days, a period longer than the necessary for the complete development of the insect. Both artificial cytokinines indeed increased the lifetime of the coffee leaves, maintaining them green and healthy. Leaves placed in the cages for oviposition were attractive to the insect, with significant number of eggs per leaf. In most cases, eggs resulted in individuals that completed the whole developmental cycle. Tests with regulator in different concentrations with healthy leaves showed efficiency. However, we believe that hormone concentrations to be used with mined leaves should be larger, because these when maintained at 10-7 M leaves did not present a satisfactory lifetime. Therefore, tests with mined leaves with different hormone concentrations should be made to find out the ideal concentration for leaf survival. In our laboratory we are successfully using 10-6 M benzyladenin for the maintenance of mined leaves.

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