In vitro anti-adhäsive und anti-inflammatorische Eigenschaften eines hydroalkoholischen Extraktes aus Tropaeolum majus L.

2019 ◽  
Author(s):  
P Vollmer ◽  
J Sendker ◽  
B Lipowicz ◽  
A Hensel
Keyword(s):  
Tropaeolum Majus

scholarly journals Ação acaricida in vitro de Tropaeolum majus sob teleóginas de Rhipicephalus (Boophilus) microplus

2010 ◽  
Vol 40 (10) ◽  
pp. 2141-2145 ◽  
Author(s):  
Felipe Lamberti Pivoto ◽  
Andréia Buzatti ◽  
Felipe da Silva Krawczak ◽  
Giovana Camillo ◽  
Luís Antônio Sangioni ◽  
...  
Keyword(s):  
Rio Grande ◽  
Boophilus Microplus ◽  
Rio Grande Do Sul ◽  
Tropaeolum Majus

O objetivo desse estudo foi o de avaliar a eficácia acaricida in vitro do extrato aquoso e etanólico a 70% da Tropaeolum majus em teleóginas do Rhipicephalus (Boophilus) microplus. Extrato das partes aéreas (caule e folhas) e de flores foram testados na concentração final de 2%. A coleta de teleóginas de Rhipicephalus (Boophilus) microplus foi realizada em propriedades do município de São Francisco de Assis e São Pedro do Sul, Rio Grande do Sul, Brasil. O extrato das partes aéreas em etanol a 70% alcançou eficácia de 99,1%. Esse estudo é o primeiro relato de ação carrapaticida de Tropaeolum majus.

Preliminary Phytochemical Screening and In vitro Antioxidant Activity of Methanolic Extract of Tropaeolum majus L. Seed

2017 ◽  
Vol 19 (3) ◽  
pp. 1-6
Author(s):  
Muhammad Hayat ◽  
Muhammad Wajid ◽  
Muhammad Iqbal ◽  
Anjum Khursheed ◽  
Muhammad Uzair ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Phytochemical Screening ◽  
Tropaeolum Majus ◽  
In Vitro Antioxidant Activity

scholarly journals Floral Biology and in vitro Germination of Nasturtium Pollen Grains Grown With Poultry Litter

2019 ◽  
Vol 11 (7) ◽  
pp. 160
Author(s):  
G. T. Zanetti ◽  
L. M. C. Davide ◽  
M. do C. Vieira ◽  
N. A. H. Zárate ◽  
A. M. T. Alovisi ◽  
...  
Keyword(s):  
Culture Media ◽  
Germination Rate ◽  
Poultry Litter ◽  
Pollen Grains ◽  
Floral Biology ◽  
Basic Knowledge ◽  
In Vitro Germination ◽  
Tropaeolum Majus ◽  
Floral Bud

The plant Tropaeolum majus, popularly known as nasturtium, presents food, ornamental and medicinal importance. Studies on floral and carpometric characteristics generate basic knowledge for areas of science such as botany, genetics and plant breeding. This work has the objectives of analyzing the effect of combinations of doses of poultry litter on floral structures and the in vitro germination of T. majus pollen grains. From the experimental matrix Plan Puebla III were defined 9 combinations of poultry litter doses incorporated and covered. The design of the experiments was entirely random, varying the replicates number. The number of stamens, petals, sepals and carpels was verified per floral bud in each treatment. The in vitro germination rate of pollen grains was estimated in 5 culture media and 2 germination times, from flower buds randomly collected in the experiment. The overall mean of stamens, sepals, petals and carpels per floral bud was 8.23; 5; 5 and 3, respectively. The combinations of poultry litter doses influenced only the number of stamens. The in vitro germination rate of T. majus pollen grains varied in the culture media. There was no association between the combinations of poultry litter doses and the in vitro germination rate of pollen grains.

scholarly journals Evaluación de citotoxicidad del isotiocianato de bencilo de Troapeolum majus L. en líneas tumorales de mama

2021 ◽  
Vol 16 (1) ◽  
pp. 23-27
Author(s):  
Clara Espinoza Silva ◽  
Miguel Quispe Solano ◽  
Nora Véliz Sedano ◽  
Lilian Baños Medina
Keyword(s):  
Cáncer De Mama ◽  
Tropaeolum Majus ◽  
Mcf 7

El mastuerzo (Tropaeolum majus L.) es una planta originaria de Perú, México y partes de América Central; en el Perú, era usada desde tiempos muy antiguos, por nuestros antepasados, para combatir distintas enfermedades. Entre sus componentes se encuentra la glucotropaeolina, que, al ser hidrolizado, forma el bencil isotiocianato (BIT) que fomenta la apoptosis celular. Debido a ello, se tuvo como objetivo; primero, evaluar el efecto citotóxico del isotiocianato de bencilo del mastuerzo expuestas a microgravedad sobre el cultivo in vitro de la línea tumoral MCF-7 de cáncer de mama. Se utilizaron extractos de isotiocianato de bencilo de mastuerzo de la planta cultivada en forma natural (T1) y; la segunda, expuesta a microgravedad en la etapa de germinación (T2), comparándose con el producto comercial (BI), la viabilidad celular se efectuó a través de la técnica del MTT realizándose diluciones de 0, 20, 40, 60, 80 y 100 uM del extracto expuesto al cultivo de células tumorales de tipo MCF-7 cáncer de mama por un tiempo de 12, 24, 48, y 60 horas lecturándose en un lector de placas de ELISA a 570 nm. Se obtuvo una DL50 entre 22,56 ± 3,24 a 19,97 ± 2,52 a las 60 horas, valores similares a las 48 horas comparadas con el control benzil de isiocianato (BI). En conclusión, no se evidencia diferencia en el efecto de la dosis letal media de todos los tratamientos en las 48 y 60 horas de incubación frente al benzil isiocianato (BI). El efecto máximo de inhibición en todas las concentraciones es apreciable a las 60 horas de incubación, afirmándose que no existe una diferencia significativa de sus actividades biológicas entre T1 y T2.

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Immunoenzymatic demonstration of the simultaneous presence of transferrin, hemopexin and albumin in a same hepatocyte and their ultrastructural localization

1978 ◽  
Vol 36 (2) ◽  
pp. 88-89
Author(s):  
M. Kraemer ◽  
J. Foucrier ◽  
J. Vassy ◽  
M.T. Chalumeau
Keyword(s):  
Plasma Proteins ◽  
Rat Hepatocytes ◽  
Ultrastructural Localization ◽  
Carrier Proteins ◽  
Normal Cells ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Monospecific Antisera ◽  
Different Levels

Some authors using immunofluorescent techniques had already suggested that some hepatocytes are able to synthetize several plasma proteins. In vitro studies on normal cells or on cells issued of murine hepatomas raise the same conclusion. These works could be indications of an hepatocyte functionnal non-specialization, meanwhile the authors never give direct topographic proofs suitable with this hypothesis.The use of immunoenzymatic techniques after obtention of monospecific antisera had seemed to us useful to bring forward a better knowledge of this problem. We have studied three carrier proteins (transferrin = Tf, hemopexin = Hx, albumin = Alb) operating at different levels in iron metabolism by demonstrating and localizing the adult rat hepatocytes involved in their synthesis.Immunological, histological and ultrastructural methods have been described in a previous work.

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