scholarly journals Evaluation of Cariogenic Bacteria

2007 ◽  
Vol 01 (01) ◽  
pp. 031-039 ◽  
Author(s):  
Fusao Nishikawara ◽  
Yoshiaki Nomura ◽  
Susumu Imai ◽  
Akira Senda ◽  
Nobuhiro Hanada

ABSTRACTObjectives : The evaluation of Mutans streptococci (MS) is one of the index for caries risk. DentocultTM and CRTTM are commercial kits to detect and evaluate MS, conveniently. However, the evaluation of MS has also been carried out simply using an instruction manual. But the instruction manual is not easy to use for evaluation of MS. The aim of this study was to examine the utility of modified Mitis-Salivalius Bacitracin (MSB) agar medium compared with MSB agar medium and commercial kits, and to establish a convenient kit (mMSB-kit) using modified MSB agar.Methods : The MS in stimulated saliva from 27 subjects were detected by MSB, modified MSB agar medium and commercial kits. Laboratory and clinically isolated strains of MS were similarly evaluated. The ratios of MS in detected bacteria were compared by ELISA.Results : The scores using an mMSB-kit on the basis of modified MSB agar medium were tabulated. Saliva samples showed different levels of MS between culture methods and the commercial kit. Some samples which were full of MS were not detected by the commercial kit. The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium, CRTTM, (P< .01) and Dentocult SMTM (P<.05).Conclusion : The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium. The mMSB-kit can be used simply, and can be an important contributor for the evaluation of MS as a caries risk factor. (Eur J Dent 2007;1:31-39)

2012 ◽  
Vol 91 (7_suppl) ◽  
pp. S52-S58 ◽  
Author(s):  
A. Ito ◽  
M. Hayashi ◽  
T. Hamasaki ◽  
S. Ebisu

If it is to be effective, preventive dental treatment must be based on an individual’s risk of caries and periodontal disease. However, hitherto, preventive treatment has been generally conducted without proper caries-risk assessment because of lack of knowledge about an individual’s caries risk. This study sought to identify high-risk adult patients and examine the effectiveness of preventive programs. Data for 442 patients from a single general practice who met the inclusion criteria were analyzed. Multiple logistic-regression analysis showed that high levels of mutans streptococci (SM) correlated with the onset of primary and secondary caries, with odds ratios of 2.34 (95%CI: 1.15-4.76, p = 0.019) and 2.22 (95%CI: 1.06-4.62, p = 0.034), respectively. Lactobacilli (LB) also correlated with secondary caries, with an odds ratio of 3.34 (95%CI: 1.35-6.85, p = 0.007). When the patients were checked after three years of a preventive program, the correlation with SM and LB disappeared. Survival analyses by Cox regression models and Kaplan-Meier methods showed that secondary caries was difficult to control by conventional preventive programs alone in patients with high levels of cariogenic bacteria. In conclusion, cariogenic bacteria play an important role in the incidence of caries, and additional intensive preventive treatment should be considered for high-risk patients.


2011 ◽  
Vol 36 (2) ◽  
pp. 185-188 ◽  
Author(s):  
Benjamin Peretz ◽  
Yardena Mazor ◽  
Nurit Dagon ◽  
Ronit Bar-Ness Greenstein

Objective: to test the association between Candida and mutans streptococci (ms), oral hygiene and caries levels and in children. Methods: 22 boys and 12 girls (age 6 to 14.5 years) participated in the study. Each participant received a toothbrush, and was asked to brush his/her teeth after proper instructions. Dental caries and oral hygiene were recorded. Candida and ms levels were determined in saliva samples. Results: Candida colonies were observed in 70.5% of the children. No association was found between Candida and caries or plaque and gingival indices. C. albicans-positive children demonstrated significantly higher brushing scores. Conclusions: Our findings may suggest that there is no clear association between Candida in saliva, and levels of cariogenic bacteria and caries risk in children.


2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Junko Tanaka ◽  
Norio Mukai ◽  
Muto Tanaka ◽  
Masahiro Tanaka

Objective. The purpose of this study was to investigate whether teeth that have undergone prosthetic restoration are under conditions that promote caries recurrence.Methods. The subjects were 20 dentate adults with both a healthy tooth and an affected tooth entirely covered with a complete cast crown in the molar regions of the same arch. The pH was measured in plaque adhering to the margin of the tooth covered with a complete cast crown and adhering to the cervicobuccal area of the natural tooth. In addition, the numbers of cariogenic bacteria (mutans streptococci and lactobacilli) were measured employing the saliva test. The relationships between the number of cariogenic bacteria and plaque pH of the natural tooth and between the number of cariogenic bacteria and plaque pH of the tooth covered with a complete cast crown were investigated.Results. The plaque pH of the tooth covered with a complete cast crown decreased as the numbers of SM and LB increased. The natural tooth were also influenced by the number of SM.Conclusion. Secondary caries are likely to develop from the marginal region of the crown in the oral cavity with a high caries risk unless a preventive program is prepared and the oral environment is improved following the program.


Author(s):  
T. N. Tserakhava ◽  
N. V. Shakavets ◽  
E. I. Melnikava ◽  
M. I. Klenovskaya ◽  
D. N. Naumovich ◽  
...  

Relevance. Prevention of caries of the first permanent molars is one of the most relevant problems in pediatricdentistry.Purpose – to develop an algorithm for prevention of first permanent molars caries in children with differentlevels of caries risk.Materials and methods. The article presents the results of the implementation of the algorithm for prevention of first permanent molars caries in children with different levels of caries risk. This algorithm includes a comprehensive assessment of the values of indices dmft, DMFT, OHI-S, and the patient's health group is also taken into account. The study involved 253 children aged 6-7 years divided into 4 groups: 3 groups of children depending on the health group and the control group. 3 subgroups were identified in each group – with a low, medium, and high caries risk. We developed preventive measures schemes were for children of each group including training in oral hygiene; controlled and home toothbrushing using fluoride-containing toothpastes; applications of varnishes containing fluoride, calcium, phosphates from 2 to 3 times a year; fissure sealing of the first permanent molars. We carried out these activities were for 24 months, and then evaluated theirs effectiveness. Children in the control group were trained in oral hygiene. The clinical effectiveness of medical prophylaxis was evaluated by changes in the above clinical indicators.Results. In group of children with medium caries risk the increase in caries was 0.09, and the reduction in caries was 89.65%. In children with a low and high caries risk no increase in caries was observed; the reduction in the intensity of caries was 100%. A significant decrease in OHI-S oral hygiene index values was noted in all groups (p < 0.05). We noted high preventive efficacy of fissures sealing in the first permanent molars. No occlusal surface caries developed in sealed fissures.Conclusions. The application of the proposed preventive schemes in patients demonstrates high efficacy of fluoride and calcium-containing varnishes and sealing the fissures of the first permanent molars. 


2005 ◽  
Vol 15 (2) ◽  
pp. 203-206 ◽  
Author(s):  
Ying Ji ◽  
Yukie Nakai ◽  
Omar Marianito Maningo Rodis ◽  
Naoyuki Kariya ◽  
Seishi Matsumura ◽  
...  

2005 ◽  
Vol 133 (7-8) ◽  
pp. 372-378 ◽  
Author(s):  
Tatjana Todorovic ◽  
Ivan Dozic ◽  
Dusan Pavlica ◽  
Dejan Markovic ◽  
Mirjana Ivanovic ◽  
...  

Saliva is a secretion of the salivary and mucous glands and is of major importance in the maintainance of oral health. Over the last few decades, saliva has been evaluated as a diagnostic fluid in medicine for determining systemic disease markers as well as for monitoring numerous drugs, narcotics, and hormones. The biochemical analysis of saliva is particularly important in dentistry. The estimation of the risk of appearance and diagnosis of disease, monitoring of disease progression, evaluation of therapy efficacy for caries, periodontitis, premalignant and malignant oral lesions, as well as infectious diseases of the oral cavity, can be assessed by analyzing different constituent: of saliva, individuals at risk of caries can be identified using test: that determine saliva flow rate, saliva buffer capacity, and colonization of the oral cavity by cariogenic bacteria. Today, these rapid and simple diagnostic tests are used routinely in caries risk determination. The study and use of saliva-based diagnostics have increased over the last few decades. Clinical testing of saliva shows much promise. However, there is a need for much additional research in this area, before the true clinical value of saliva as a diagnostic fluid in dentistry can be determined.


2005 ◽  
Vol 29 (4) ◽  
pp. 329-333 ◽  
Author(s):  
Luis Alejandro Aguilera Galaviz ◽  
Gloria Premoli ◽  
Anajulia Gonzalez ◽  
Rafael Aguilar Rodriguez

Lactobacullus sp. and S. mutans are microorganisms with cariogenic capacity, however, their presence do not determine the presence of dental caries.We evaluated the relationship between the presence of Lactobacillus sp. and S. mutans and dental caries in a schoolchildren population. The relation PIDMFT have a value of significance p = 0.001489. In dental caries risk evaluation, the S. mutans and Lactobacillus sp. detection in saliva is a good predictor and contributing to the caries development.


2018 ◽  
Vol 7 (3) ◽  
pp. 411-429 ◽  
Author(s):  
Elissa J. Hamlat ◽  
Hannah R. Snyder ◽  
Jami F. Young ◽  
Benjamin L. Hankin

Evidence suggests that early pubertal timing may operate as a transdiagnostic risk factor (i.e., shared across syndromes of psychopathology) for both genders. The current study examined associations between pubertal timing and dimensional psychopathology, structured across different levels of three organizational models: (a) DSM-based syndrome model, (b) traditional model of internalizing and externalizing factors, and (c) bifactor (p factor) model, which includes a general psychopathology factor as well as internalizing- and externalizing-specific factors. For study analyses, 567 youth-parent pairs completed psychopathology measures when youths (55.5% female) were 13.58 years old ( SD = 2.37, range = 9–17 years). Findings across all models revealed that early pubertal timing served as a transdiagnostic risk factor and also displayed some syndrome-specific associations. Gender did not moderate any relationships between pubertal timing and psychopathology. Study findings reinforce the importance of examining risk across different levels of psychopathology conceptualization and analysis.


2018 ◽  
Vol 12 (supplement_1) ◽  
pp. S561-S562
Author(s):  
E Rodrigues ◽  
N Laranjeira ◽  
G Nunes ◽  
R Barosa ◽  
M Patita ◽  
...  

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