Inflammatory response of two invasive techniques in the mouse with collagenase induced tendinopathy

Abstract Background Percutaneous needle electrolysis (PNE) is a physiotherapy technique which has demonstrated its effectiveness for the treatment of tendinopathies and muscle pathologies. All the authors consulted base its therapeutic effect on the capacity to produce an important inflammatory response during the initial phase of the regenerative process. However, presently, the histological effects of the technique on tendinopathies are unknown. The aim of this study was to histologically examine the inflammatory response provoked by the PNE versus dry needling (DN) in mice with collagenase-induced tendinopathy. Material and Methods In order to achieve the proposed aim, a murine model was used with a total of 18 C57BL/6J mice. One week prior to the intervention, collagenase-induced tendinopathy was performed in the common calcaneal tendons of all mice. Once the pathology was established, they received DN in the left common calcaneus tendon, whereas, on the right, they received treatment with PNE. Groups of animals were sacrificed at 3, 7 and 14 days to evaluate the histological evolution of injuries. Samples of the common calcaneal tendon were taken and were later fixed in formalin, processed, and included in paraffin blocks. Sections of 3 microns thick were performed, which were dyed using hematoxylin-eosin for conventional histopathological examination. Results In both groups, tissue damage was observed with collagen fragmentation and the presence of fibroblasts. Dry needling of the common calcaneal tendon induced the presence of an acute inflammatory infiltrate (characterized by the presence of polymorphonuclear neutrophils and macrophages) at the level of the peritenon and adjacent fat tissue which was detectable after day 3, and notable after days 7 and 14. The application of PNE induced a similar effect, although on day 14, the inflammatory infiltrate of PNE was more evident, compared to DN. Conclusion The proposed in vivo murine model has demonstrated to be useful for the study of the evolution of the first phases of the regenerative process induced by both techniques. The histopathological results reveal that PNE generates a pro-inflammatory stimulus which is superior to DN on day 14.

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Histopathological analysis of the inflammatory response of two invasive techniques in the calcaneal tendon of a mouse

Revista Fisioterapia Invasiva ◽

10.1055/s-0039-3401870 ◽

2019 ◽

Vol 02 (02) ◽

pp. 091-091

Author(s):

Medina i Mirapeix F. ◽

García Vidal JA ◽

Escolar Reina P. ◽

Martínez Cáceres CM

Keyword(s):

Inflammatory Response ◽

Murine Model ◽

Inflammatory Infiltrate ◽

Histopathological Examination ◽

Polymorphonuclear Neutrophils ◽

Histopathological Analysis ◽

Dry Needling ◽

Calcaneal Tendon ◽

The Common

Abstract Background and Aim Percutaneous needle electrolysis (PNE) is a therapeutic tool which has demonstrated its effectiveness of the treatment of tendinopathies and muscle problems. Previous authors have based the therapeutic effect of the same on the ability to provoke an important inflammatory response. However, there is a scarcity of basic research directed at understanding its effects on a cellular and histological level. The aim of this study was to histopathologically compare the inflammatory response provoked by PNE versus dry needling (DN) in a healthy animal model. Material and Methods For the proposed study aim a murine model was used with a total of 18 C57BL/6J mice. All animals received an application of DN in the left common calcaneal tendon, whereas on the right, treatment with PNE was applied (3 impacts of 3mA during 3 seconds). Groups of animals were sacrificed at 3, 7 and 15 days to evaluate the histopathological evolution of the lesions. Samples of the common calcaneal tendon were taken and fixed in commercial formalin at 4% tamponed during 24 hours, processed and included in paraffin blocks. Sections of 3 microns thick were performed which were dyed with hematoxylin-eosin for conventional histopathological examination. Results Dry needling of the common calcaneal tendon induced the presence of an acute inflammatory infiltrate (characterized by the presence of polymorphonuclear neutrophils and macrophages) in the peritenon and adjacent fat tissue which was detectable after the third day, and which was most notable on days 7 and 14. The application of PNE induced a pattern with a similar behavior, however on day 14, the inflammatory infiltrate of PNE was more evident compared with DN. Conclusion The proposed in vivo murine model has been useful for the study of the first phases of the regeneration process induced by both techniques. The histopathological results show that PNE generates a superior stimulus compared to DN on day 14.

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In vivo evaluation of a recombinant N-acylhomoserine lactonase formulated in a hydrogel using a murine model infected with MDR Pseudomonas aeruginosa clinical isolate, CCASUP2

AMB Express ◽

10.1186/s13568-021-01269-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Masarra M. Sakr ◽

Walid F. Elkhatib ◽

Khaled M. Aboshanab ◽

Eman M. Mantawy ◽

Mahmoud A. Yassien ◽

...

Keyword(s):

Survival Rate ◽

Murine Model ◽

Histopathological Examination ◽

Healing Process ◽

Bacterial Count ◽

Treated Group ◽

Control Group ◽

In Vivo Evaluation ◽

Systemic Spread

AbstractFailure in the treatment of P. aeruginosa, due to its broad spectrum of resistance, has been associated with increased patient mortality. One alternative approach for infection control is quorum quenching which was found to decrease virulence of such pathogen. In this study, the efficiency of a recombinant Ahl-1 lactonase formulated as a hydrogel was investigated to control the infection of multidrug resistant (MDR) P. aeruginosa infected burn using a murine model. The recombinant N-acylhomoserine lactonase (Ahl-1) was formulated as a hydrogel. To test its ability to control the infection of MDR P. aeruginosa, a thermal injury model was used. Survival rate, and systemic spread of the infection were evaluated. Histopathological examination of the animal dorsal skin was also done for monitoring the healing and cellular changes at the site of infection. Survival rate in the treated group was 100% relative to 40% in the control group. A decrease of up to 3 logs of bacterial count in the blood samples of the treated animals relative to the control group and a decrease of up to 4 logs and 2.3 logs of bacteria in lung and liver samples, respectively were observed. Histopathological examination revealed more enhanced healing process in the treated group. Accordingly, by promoting healing of infected MDR P. aeruginosa burn and by reducing systemic spread of the infection as well as decreasing mortality rate, Ahl-1 hydrogel application is a promising strategy that can be used to combat and control P. aeruginosa burn infections.

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N,N-Dimethylformamide Prevents LPS-Induced Preterm Birth in a Murine Model by Suppressing the Inflammatory Response

10.21203/rs.3.rs-969393/v1 ◽

2021 ◽

Author(s):

Zeng-Hui Wei ◽

Oluwabukola Salami ◽

Jagadish Koya ◽

Swapna Munnangi ◽

Ryan Pekson ◽

...

Keyword(s):

Preterm Birth ◽

Murine Model ◽

Raw 264.7 Cells ◽

New Approach ◽

Affect Cell Viability ◽

Pregnant Mice ◽

Inflammatory Drugs ◽

The Common ◽

Anti Inflammatory

Abstract Preterm birth accounts for the majority of perinatal mortality worldwide and there remains no FDA-approved drug to prevent it. Recently, we discovered that the common drug excipient, N,N-dimethylacetamide (DMA), prevents inflammation–induced preterm birth in mice by inhibiting NF-κB. Since we reported this finding it has come to light that a group of widely used, structurally related aprotic solvents, including DMA, N-methyl-2-pyrrolidone (NMP) and dimethylformamide (DMF), have anti-inflammatory efficacy. We show here that DMF suppresses LPS-induced TNFα secretion from RAW 264.7 cells and IL-6 and IL-8 secretion from HTR-8 cells at concentrations that do not significantly affect cell viability. In vivo, DMF decreases LPS-induced inflammatory cell infiltration and expression of TNFα and IL-6 in the placental labyrinth, all to near baseline levels. Finally, DMF decreases the rate of preterm birth in LPS-induced pregnant mice (P<.0001) and the rate at which pups are spontaneously aborted (P<.0001). In summary, DMF, a widely used solvent structurally related to DMA and NMP, prevents LPS-induced preterm birth in a murine model without overt toxic or teratogenic effects. Re-purposing the DMA/DMF/NMP family of small molecules as anti-inflammatory drugs is a promising new approach to preventing inflammation–induced preterm birth and potentially other inflammatory disorders as well.

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Experimental Encephalitozoonosis in Neonatal Dogs

Veterinary Pathology ◽

10.1177/030098588702400201 ◽

1987 ◽

Vol 24 (2) ◽

pp. 99-108 ◽

Cited By ~ 19

Author(s):

J. R. Szabo ◽

J. A. Shadduck

Keyword(s):

Inflammatory Response ◽

Inflammatory Infiltrate ◽

B Lymphocyte ◽

Plasma Cells ◽

Protozoan Parasite ◽

Encephalitozoon Cuniculi ◽

Large Component ◽

Blastogenic Response ◽

Postnatal Infection

The in vivo infection of neonatal dogs by the microsporidian protozoan parasite, Encephalitozoon cuniculi, was studied. Microscopic examination of tissues from infected animals showed granulomatous nephritis, meningoencephalitis, hepatitis, and pneumonitis. A large component of the inflammatory infiltrate consisted of plasma cells and lymphocytes. In addition, hyperplasia of B-lymphocyte-dependent regions of lymph nodes and erythrophagocytosis were consistently seen in infected dogs. Infected dogs developed lymphocytosis, hypergammaglobulinemia, anti-encephalitozoon antibodies, and an antigen-specific blastogenic response to E. cuniculi spores. Lymphocyte blastogenic responses to the lectin phytohemagglutinin A (PHA) were depressed compared to controls. Dogs dying during the 2-month experimental trial were bacteremic. The findings of these experiments suggest that postnatal infection results in a demonstrable although seemingly ineffective immune and inflammatory response without detectable clinical disease.

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In vivo efficacy and toxicity of synthesized nano-β-tricalcium phosphate in a rabbit tibial defect model

Toxicology Research and Application ◽

10.1177/2397847318819499 ◽

2018 ◽

Vol 2 ◽

pp. 239784731881949

Author(s):

Jer Ping Ooi ◽

Shah Rizal Kasim ◽

Rumaizi Bin Shaari ◽

Nor Aini Saidin

Keyword(s):

Inflammatory Response ◽

Tricalcium Phosphate ◽

Histopathological Examination ◽

Healing Process ◽

Control Group ◽

Precipitation Process ◽

Defect Model ◽

Defect Site ◽

Tibial Defect

Previous studies of the biocompatibility of β-tricalcium phosphate (β-TCP) focused on bulk-sized β-TCP, and little is known about the biocompatibility of nano β-TCP particles (nβ-TCP). The objectives of this study were to synthesize nβ-TCP particles and determine their efficacy in a rabbit tibial defect model. The nβ-TCP particles were first synthesized using a wet chemical precipitation process. The particles were then implanted in the left tibia of New Zealand white rabbits, and the defect site healing was evaluated for a period of 16 weeks using radiography, computed tomography, and histology. Data were compared with those of a sham (empty) control. Results showed that the defect site treated with nβ-TCP particles did not heal completely after 16 weeks, whereas full cortical bone recovery was observed in the sham control group of rabbits. Histopathological examination showed that the nβ-TCP particles caused an excessive and prolonged inflammatory response by the host. The nano-scaled size and biodegradability of the synthesized nβ-TCP particles may have been responsible for this progressive and extended inflammatory response, which delayed the bone healing process. The underlying mechanism for this effect remains unclear and warrants further investigation.

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Efficacy of ceftolozane in a murine model of Pseudomonas aeruginosa acute pneumonia: in vivo antimicrobial activity and impact on host inflammatory response

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dks343 ◽

2012 ◽

Vol 68 (1) ◽

pp. 177-183 ◽

Cited By ~ 19

Author(s):

C. Jacqueline ◽

A. Roquilly ◽

C. Desessard ◽

D. Boutoille ◽

A. Broquet ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Inflammatory Response ◽

Murine Model ◽

Acute Pneumonia ◽

Host Inflammatory Response

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In Vivo Evaluation of a Recombinant N-acylhomoserine Lactonase Formulated in a Hydrogel Using a Murine Model Infected With Mdr Pseudomonas Aeruginosa Clinical Isolate, CCASUP2

10.21203/rs.3.rs-209973/v1 ◽

2021 ◽

Author(s):

Masarra M. Sakr ◽

Walid F. Elkhatib ◽

Khaled Aboshanab ◽

Eman M. Mantawy ◽

Mahmoud A. Yassien ◽

...

Keyword(s):

Survival Rate ◽

Murine Model ◽

Histopathological Examination ◽

Healing Process ◽

Bacterial Count ◽

Treated Group ◽

Control Group ◽

In Vivo Evaluation ◽

Systemic Spread

Abstract Failure in the treatment of P. aeruginosa, due to its broad spectrum of resistance, has been associated with increased patient mortality. One alternative approach for infection control is quorum quenching which was found to decrease virulence of such pathogen. In this study, the efficiency of a recombinant Ahl-1 lactonase formulated as a hydrogel was investigated to control the infection of multidrug resistant (MDR) P. aeruginosa infected burn using a murine model. The recombinant N-acylhomoserine lactonase (Ahl-1) was formulated as a hydrogel. To test its ability to control the infection of MDR P. aeruginosa, a thermal injury model was used. Survival rate, and systemic spread of the infection were evaluated. Histopathological examination of the animal dorsal skin was also done for monitoring the healing and cellular changes at the site of infection. Survival rate in the treated group was 100% relative to 40% in the control group. A decrease of up to 3 logs of bacterial count in the blood samples of the treated animals relative to the control group and a decrease of up to 4 logs and 2.3 logs of bacteria in lung and liver samples, respectively were observed. Histopathological examination revealed more enhanced healing process in the treated group. Accordingly, by promoting healing of infected MDR P. aeruginosa burn and by reducing systemic spread of the infection as well as decreasing mortality rate, Ahl-1 hydrogel application is a promising strategy that can be used to combat and control P. aeruginosa burn infections.

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