Computer-Aided GC/MS Analysis of Essential Oils in Complex Vegetable Drugs for Quality Control

The study was aimed to investigate the chemical components of stem and fruit volatile oils of Piper. maingayi Hk. with their antioxidant and antityrosinase activities. GC and GC-MS analysis of the essential oils obtained from the fresh stem and fruit of P. maingayi Hk. resulted in the identification of 34 and 18 components accounting for 83.6% and 78.7% of the total amount, respectively. Sesquiterpene hydrocarbons and oxygenated sesquiterpenes were the most highly represented classes as the former ranging from 64.7% to 70.7%, and the latter varying from 7.4% to 8.6%. The main constituents of the stem oil were: β-caryophyllene (26.2%), a-cedrene (8.4%), caryophyllene oxide (6.7%) and cis-calamenene (6.2%), while the fruit oil was dominated by δ-cadinene (22.6%), β-caryophyllene (18.8%), a-copaene (11.2%) and a-cadinol (7.1%). The stem and fruit of P. maingayi oils showed significant value for antioxidant and antityrosinase activities.

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Medicinal and pesticidal potentials of the constituents of the essential oil from Adenia cissampeloides leaves

Kemija u industriji ◽

10.15255/kui.2018.027 ◽

2019 ◽

Vol 68 (1-2) ◽

pp. 7-21

Author(s):

Modupe Ogunlesi ◽

Wesley Okiei ◽

Edith Ofor ◽

Anthony Eniola

Keyword(s):

Essential Oil ◽

Alternative Medicine ◽

Essential Oils ◽

Linolenic Acid ◽

Hexadecanoic Acid ◽

Ms Analysis ◽

Hexahydrofarnesyl Acetone ◽

Potential Applications

Adenia cissampeloides (Planch. ex Hook.) Harms is used in alternative medicine primarily for the management of hypertension and several other diseases. Isolation and GC-MS analysis of five essential oils collected fractionally and over 4 h by hydrodistillation from the dried leaves were carried out. The constituents included phytol, α-linolenic acid, n-hexadecanoic acid, hexahydrofarnesyl acetone, (13S)-8,13-epoxy-labd-14-ene, kaur-16-ene, guaiol, α-gurjunene, and α-elemene. Several bioactivities of the constituents as well as their potential applications in medicine and the pharmaceutical and pesticidal industries are discussed. Essential oils from some other plants have several constituents in common with those isolated from A. cissampeloides. The bioactivities of such plants are used to suggest other possible applications of A. cissampeloides.

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Comparison of chemical compositions of essential oils isolated by hydrodistillation from wild thyme (Thymus serpyllum L.) with use of Deryng and Clevenger apparatus

Herba Polonica ◽

10.2478/hepo-2014-0006 ◽

2014 ◽

Vol 60 (2) ◽

pp. 7-17 ◽

Cited By ~ 4

Author(s):

Aneta Wesołowska ◽

Monika Grzeszczuk ◽

Dorota Jadczak

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Methyl Ether ◽

The Other ◽

Chemical Compositions ◽

Distillation Apparatus ◽

Ms Analysis ◽

Wild Thyme ◽

Thymus Serpyllum

Abstract The aim of the studies conducted in 2012-2013 was to compare the chemical composition of essential oils isolated from wild thyme (Thymus serpyllum L.) by hydrodistillation in Deryng and Clevenger apparatus. GC-MS analysis of the isolated oils revealed that carvacrol (42.81-45.24%), γ-terpinene (7.68-9.04%), β-caryophyllene (5.28-9.10%), β-bisabolene (5.76-6.91%) and carvacrol methyl ether (4.92-6.09%) were the major components of all the samples. On the basis of the obtained data it was proved that the type of distillation apparatus had no significant effect on the content of the main essential oil constituents of wild thyme. However, based on the means for both years of the study it was proved that hydrodistillation in Deryng apparatus was more effective for carvacrol concentration, while in Clevenger apparatus - for y-terpinene and carvacrol methyl ether concentration. The type of distillation apparatus had no significant effect on the content of the other essential oil constituents.

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GC/MS Analysis and Antimicrobial Activity of the Essential Oil of Fresh Leaves of Eucalytus Globulus, and Leaves and Stems of Smyrnium Olusatrum from Constantine (Algeria)

Natural Product Communications ◽

10.1177/1934578x1000501031 ◽

2010 ◽

Vol 5 (10) ◽

pp. 1934578X1000501 ◽

Cited By ~ 5

Author(s):

Habiba Daroui-Mokaddem ◽

Ahmed Kabouche ◽

Mabrouka Bouacha ◽

Boudjemaa Soumati ◽

Aida El-Azzouny ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Eucalyptus Globulus ◽

Bacterial Strains ◽

Ms Analysis ◽

Wide Range ◽

Main Components ◽

Type Apparatus

The essential oils of fresh leaves of Eucalyptus globulus L. (Myrtaceae), and leaves and stems of Smyrnium olusatrum L. (Apiaceae), obtained by hydrodistillation in a Clevenger-type apparatus, were analyzed by GC/MS. Twenty compounds were characterized, representing 98.3% of the essential oil of E. globulus, with 1,8-cineole (48.6%), globulol (10.9%), trans-pinocarveol (10.7%), and α-terpineol (6.6%) as main components, while thirty-three compounds were characterized, representing 94.3% of the essential oil of S. olusatrum, with sabinene (27.1%), curzerene (13.7%), methyl-1-benzyl-2-oxocyclooctane carboxylate (12.3%), α-pinene (7.2%), cryptone (7.1%) and β-pinene (5.7%) as the major components. The essential oils of E. globulus and S. olusatrum were tested against a wide range of fungal and bacterial strains. Both oils showed significant antimicrobial activity.

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GC-MS Analysis of the Essential Oils of Roots and Leaves ofFoeniculum vulgareMill

Journal of Essential Oil Bearing Plants ◽

10.1080/0972060x.2007.10643516 ◽

2007 ◽

Vol 10 (1) ◽

pp. 36-40 ◽

Cited By ~ 4

Author(s):

Faraz Mojab ◽

Katayun Javidnia ◽

Bahman Nickavar ◽

Darab Yazdani

Keyword(s):

Essential Oils ◽

Ms Analysis

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Pesticide and Plasticizer Residues in Citrus Essential Oils from Different Countries

Natural Product Communications ◽

10.1177/1934578x1000500838 ◽

2010 ◽

Vol 5 (8) ◽

pp. 1934578X1000500 ◽

Cited By ~ 1

Author(s):

Giuseppa Di Bella ◽

Vincenzo Lo Turco ◽

Rossana Rando ◽

Gabriella Arena ◽

Donatella Pollicino ◽

...

Keyword(s):

Quality Control ◽

Essential Oils ◽

Organochlorine Pesticides ◽

Production Cycle ◽

Improper Use ◽

Citrus Essential Oils

Residue analyses are very important in the quality control of citrus essential oils. Organophosphorus and organochlorine pesticides, phosphorated plasticizers and chloroparaffins contamination were investigated by HRGC with FPD and ECD detectors in 120 citrus essential oils produced in Italy and in 70 from other countries in the crop year 2006-2007. Results showed that the largest pesticide quantities were found in oils from Brazil and Spain. The presence of such residues might be the result of an improper use of pesticide in citrus growing or of previous contamination of the extractors. However, the pesticide levels showed a measurable decrease in relation to past production years. The absence of phosporated plasticizers and chloroparaffins can be the result of either improvement of the procedures used during the production cycle or in the storage of the essential oils compared with previous years.

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Volatiles of Tanacetum macrophyllum Obtained by Different Extraction Methods

Natural Product Communications ◽

10.1177/1934578x1801300727 ◽

2018 ◽

Vol 13 (7) ◽

pp. 1934578X1801300

Author(s):

Biljana Nikolić ◽

Milić Matović ◽

Marina Todosijević ◽

Jovana Stanković ◽

Mirjana Cvetković ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Extraction Methods ◽

Static Headspace ◽

Oxygenated Monoterpenes ◽

Ms Analysis ◽

Sesquiterpene Hydrocarbons ◽

Germacrene D ◽

Headspace Gc

Terpene compounds of Tanacetum macrophyllum as 1) essential oils, obtained by hydrodistillation (HD), 2) essential oil extracts, obtained by simultaneous hydrodistillation and extraction (SDE) and 3) volatiles, obtained by Static Headspace GC-MS analysis (HS) were processed. Monoterpenes were the most dominant (49.2%, 49.5% and 90.4%, respectively). Profiles of essential oils obtained by HD and SD were quite similar, with oxygenated monoterpenes (39.3% and 39.4%) being the most abundant. In HS volatiles oxygenated monoterpenes also dominated (57.4%). Total sesquiterpenes were abundant in HD and SDE volatiles (38.2% and 39.2%, resp.), where sesquiterpene hydrocarbons were prevalent (27.3% and 28.7%, resp.). Germacrene D was dominant in HD and SDE oils (22.0% and 23.3%, resp.) and 1,8-cineole in HS volatiles (34.3%). To our knowledge, this is the first use of Headspace technique on T. macrophyllum. Furthermore, this is the first comparison of different techniques of volatile extraction in T. macrophyllum.

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Chemical Compositions and Antimicrobial Activity of the Essential Oils of Piper abbreviatum, P. erecticaule and P. lanatum (Piperaceae)

Natural Product Communications ◽

10.1177/1934578x1400901235 ◽

2014 ◽

Vol 9 (12) ◽

pp. 1934578X1400901 ◽

Cited By ~ 1

Author(s):

Wan Mohd Nuzul Hakimi Wan Salleh ◽

Farediah Ahmad ◽

Khong Heng Yen

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Antimicrobial Agents ◽

Antimicrobial Activities ◽

Dilution Method ◽

Chemical Compositions ◽

Gram Positive Bacteria ◽

Aerial Parts ◽

Ms Analysis ◽

Weak Activity

The study was designed to examine the chemical composition and antimicrobial activities of essential oils extracted from the aerial parts of three Piper species: Piper abbreviatum, P. erecticaule and P. lanatum, all from Malaysia. GC and GC/MS analysis showed qualitative and quantitative differences between these oils. GC and GC-MS analysis of P. abbreviatum, P. erecticaule and P. lanatum oils resulted in the identification of 33, 35 and 39 components, representing 70.5%, 63.4% and 78.2% of the components, respectively. The major components of P. abbreviatum oil were spathulenol (11.2%), ( E)-nerolidol (8.5%) and β-caryophyllene (7.8%), whereas P. erecticaule oil mainly contained β-caryophyllene (5.7%) and spathulenol (5.1%). Borneol (7.5%), β-caryophyllene (6.6%) and α-amorphene (5.6%) were the most abundant components in P. lanatum oil. Antimicrobial activity was carried out using disc diffusion and broth micro-dilution method against nine microorganisms. All of the essential oils displayed weak activity towards Gram-positive bacteria with MIC values in the range 250–500 μg/mL. P. erecticaule oil showed the best activity on Aspergillus niger (MIC 31.3 μg/mL), followed by P. lanatum oil (MIC 62.5 μg/mL). This study demonstrated that the essential oils have potential as antimicrobial agents and may be useful in the pharmaceutical and cosmetics industries.

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