scholarly journals Comparison of chemical compositions of essential oils isolated by hydrodistillation from wild thyme (Thymus serpyllum L.) with use of Deryng and Clevenger apparatus

2014 ◽  
Vol 60 (2) ◽  
pp. 7-17 ◽  
Author(s):  
Aneta Wesołowska ◽  
Monika Grzeszczuk ◽  
Dorota Jadczak
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Methyl Ether ◽  
The Other ◽  
Chemical Compositions ◽  
Distillation Apparatus ◽  
Ms Analysis ◽  
Wild Thyme ◽  
Thymus Serpyllum

Abstract The aim of the studies conducted in 2012-2013 was to compare the chemical composition of essential oils isolated from wild thyme (Thymus serpyllum L.) by hydrodistillation in Deryng and Clevenger apparatus. GC-MS analysis of the isolated oils revealed that carvacrol (42.81-45.24%), γ-terpinene (7.68-9.04%), β-caryophyllene (5.28-9.10%), β-bisabolene (5.76-6.91%) and carvacrol methyl ether (4.92-6.09%) were the major components of all the samples. On the basis of the obtained data it was proved that the type of distillation apparatus had no significant effect on the content of the main essential oil constituents of wild thyme. However, based on the means for both years of the study it was proved that hydrodistillation in Deryng apparatus was more effective for carvacrol concentration, while in Clevenger apparatus - for y-terpinene and carvacrol methyl ether concentration. The type of distillation apparatus had no significant effect on the content of the other essential oil constituents.

Chemical Composition And In-Vitro Antimicrobial Activity Of The Essential Oils Of Three Greek Achillea Species

2002 ◽  
Vol 57 (3-4) ◽  
pp. 287-290 ◽  
Author(s):  
Prokopios Magiatis ◽  
Alexios-Leandros Skaltsounis ◽  
Ioanna Chinou ◽  
Serkos A. Haroutounian
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Fungi ◽  
The Other ◽  
Gram Negative ◽  
In Vitro Antimicrobial Activity ◽  
Ms Analysis

The chemical composition of the essential oils of Achillea holosericea, Achillea taygetea, Achillea fraasii was determined by GC/MS analysis. Among the ninety-five assayed constituents, camphor, borneol and 1,8-cineol were found to be the major components. The in-vitro antimicrobial activity of these essential oils was evaluated against six bacteria indicating that the first is totally inactive, while the other two possess moderate to strong activities mainly against the Gram negative strains. The essential oil of A. fraasii was also active against the tested pathogenic fungi

scholarly journals Comparison of the Chemical Composition and Antimicrobial Activity of Thymus serpyllum Essential Oils

2015 ◽  
Vol 43 (2) ◽  
pp. 432-438 ◽  
Author(s):  
Aneta WESOŁOWSKA ◽  
Monika GRZESZCZUK ◽  
Dorota JADCZAK ◽  
Paweł NAWROTEK ◽  
Magdalena STRUK
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Micrococcus Luteus ◽  
Gas Chromatography Mass Spectrometry ◽  
E Coli ◽  
Thymus Serpyllum ◽  
Main Components ◽  
Total Oil

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Thymus serpyllum and Thymus serpyllum‘Aureus’ has been investigated by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds (99.67% of the total oil) wereidentified in the essential oil of T. serpyllum. The main components found in the oil were carvacrol (37.49%), -terpinene (10.79%), -caryophyllene (6.51%), p-cymene (6.06%), (E)--ocimene (4.63%) and -bisabolene (4.51%). Similarly, carvacrol (44.93%), -terpinene(10.08%), p-cymene (7.39%) and -caryophyllene (6.77%) dominated in the oil of T. serpyllum ‘Aureus’. A total of forty three compounds wereidentified in this oil, representing 99.49% of the total oil content. On the basis of the obtained data it was proved that the content of 1-octen-3-ol,eucalyptol, (Z)--ocimene, (E)--ocimene, -terpinene, carvacrol methyl ether, germacrene D and -bisabolene was significantly higher for T.serpyllum while T. serpyllum ‘Aureus’ was characterized by a significantly higher content of 3-octanone, 3-octanol, p-cymene, borneol andcarvacrol. The isolated essential oils were evaluated for their antimicrobial activity against nine reference strains (Escherichia coli, Staphylococcusaureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Bacillus cereus, Micrococcus luteus, Proteus vulgaris and Candidaalbicans) by the microdilution technique. Based on this test, the minimum inhibitory concentrations (MIC) of essential oil were calculated. Thevolatile oil obtained from T. serpyllum showed the highest antimicrobial activity relative to the strain of E. coli (MIC=0.025 μL/mL) and to theyeast C. albicans (MIC=0.05 μL/mL). Similarly, a significant antimicrobial activity exhibited T. serpyllum ‘Aureus’ essential oil, although the MICvalues obtained in that case for E. coli and C. albicans strains were twice as high and were respectively 0.05 μL/mL and 0.1 μL/mL.

scholarly journals Chemical Compositions of the Bark Essential Oils of Croton Monteverdensis and Croton Niveus from Monteverde, Costa Rica

2006 ◽  
Vol 1 (7) ◽  
pp. 1934578X0600100 ◽  
Author(s):  
William N. Setzer
Keyword(s):  
Costa Rica ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Chemical Compositions ◽  
Medicinal Uses ◽  
Oil Components

The essential oils of the barks of Croton monteverdensis Huft and C. niveus Jacq. (Euphorbiaceae) from Monteverde, Costa Rica, were isolated by hydrodistillation in yields of 0.03% and 0.10%, respectively. The chemical composition of the oils was determined by GC–MS. The most abundant essential oil components of C. monteverdensis were α-pinene (17.1%), β-pinene (10.5%), and linalool (8.3%), while C. niveus bark oil was made up largely of α-pinene (14.4%), 1,8-cineole (11.6%), and borneol (8.5%). The major components account for the fragrances and are consistent with the traditional medicinal uses of these plants.

scholarly journals Essential Oils of Morus alba and M. nigra Leaves: Effect of Drying on the Chemical Composition

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200
Author(s):  
Niko S. Radulović ◽  
Vojkan M. Miljković ◽  
Marko Z. Mladenović ◽  
Goran S. Nikolić
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Morus Alba ◽  
The Other ◽  
Rare Plant ◽  
Drying Process ◽  
Geranyl Acetone ◽  
Peak Areas ◽  
Plant Metabolite

Detailed GC and GC/MS analyses of essential-oil samples obtained by hydrodistillation of Morus alba L. and M. nigra L. leaves (four samples) allowed the identification of 131 constituents representing 95.1 – 96.4% of the total GC peak areas. The most abundant compounds classes were alkanes, diterpenoids, carotenoid-derived compounds and fatty acid-related constituents with trans-phytol (7.9 – 71.2%), ( E,E)-geranyl linalool (0.2 – 8.0%), ( Z)-bovolide (8.1%) and n-chain alkanes (in total, 17.5 – 52.4%) as the major constituents of the analyzed samples. In general, there were only quantitative differences noted between M. nigra essential oils from fresh and dry leaves. The most discernable changes included a variation in the content of the major constituents (e.g. the relative amount of trans-phytol and the total amount of alkanes decreased by 5.8% and ≈ 2%, respectively, while that of ( E,E)-geranyl linalool increased by 7.8%). On the other hand, the composition of M. alba essential oil was much more significantly affected by the drying process. The highest quantitative differences were noted for trans-phytol, geranyl acetone, and all isomers of 4,6,8-megastigmatrien-3-one. Also, a rare plant metabolite, ( Z)-bovolide, characteristic of leaf senescence, was only identified (8.1%) in the essential oil of M. alba dried leaves.

scholarly journals Chemical Composition of Essential Oils from the Needles and Branch of the Abies delaveyi Franch subsp. fansipanensis (Q. P. Xiang&al) Rushforth in Lao Cai Province

2021 ◽  
Vol 37 (4) ◽  
Author(s):  
Tran Huy Thai ◽  
Nguyen Thi Hien ◽  
Nguyen Duy Manh ◽  
Dinh Thi Thu Thuy ◽  
Nguyen Trung Thanh
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Chemical Constituents ◽  
Natural Park ◽  
Ms Analysis ◽  
Lao Cai

The essential oils from the needles and branch of the Abies delaveyi subsp. fansipanensis was collected in Hoang Lien Natural Park, Lao Cai province and was obtained by steam distilation and the yields of essential oils were 0.27% and 0.08% from air-dry material. By the using GC/MS analysis, there are 44 and 52 constituents from needles and branch were identified and accounting 97.75% and 96.86% essential oil. The main constituents of needles essential oil were α-pinene (22.28%), β-phellandrene (17.80%), β-pinene (7.87%), α-cadinol (6.53%), myrcene (4.35%), δ-cadinene (4.39%). The main constituents of branchs essential oil were β-phellandrene (18.0%), α-pinene (10.95%), myrcene (10.76%), β-pinene (6.71%), abienol (6.71%). α-cadinol (3.31%). This is the first study on the chemical constituents of the essential oils from the needles and branchs of Abies delaveyi subsp. fansipanensis in Vietnam.

Chemical composition of the essential oils of four Polyalthia species from Malaysia

2020 ◽  
Vol 75 (11-12) ◽  
pp. 473-478
Author(s):  
Natasa Mohd Shakri ◽  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Shamsul Khamis ◽  
Nor Azah Mohamad Ali ◽  
Shazlyn Milleana Shaharudin
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Folk Medicine ◽  
Principal Component ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Gastrointestinal Ulcer ◽  
Germacrene D

AbstractPolyalthia is one of the largest genera in the Annonaceae family, and has been widely used in folk medicine for the treatment of rheumatic fever, gastrointestinal ulcer, and generalized body pain. The present investigation reports on the extraction by hydrodistillation and the composition of the essential oils of four Polyalthia species (P. sumatrana, P. stenopetalla, P. cauliflora, and P. rumphii) growing in Malaysia. The chemical composition of these essential oils was determined by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The multivariate analysis was determined using principal component analysis (PCA) and hierarchical clustering analysis (HCA) methods. The results revealed that the studied essential oils are made up principally of bicyclogermacrene (18.8%), cis-calamenene (14.6%) and β-elemene (11.9%) for P. sumatrana; α-cadinol (13.0%) and δ-cadinene (10.2%) for P. stenopetalla; δ-elemene (38.1%) and β-cubebene (33.1%) for P. cauliflora; and finally germacrene D (33.3%) and bicyclogermacrene for P. rumphii. PCA score and HCA plots revealed that the essential oils were classified into three separated clusters of P. cauliflora (Cluster I), P. sumatrana (Cluster II), and P. stenopetalla, and P. rumphii (Cluster III) based on their characteristic chemical compositions. Our findings demonstrate that the essential oil could be useful for the characterization, pharmaceutical, and therapeutic applications of Polyalthia essential oil.

scholarly journals Essential Oil of Algerian Eryngium campestre: Chemical Variability and Evaluation of Biological Activities

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2575 ◽  
Author(s):  
Medbouhi ◽  
Benbelaïd ◽  
Djabou ◽  
Beaufay ◽  
Bendahou ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Biological Activities ◽  
In Vitro Study ◽  
Chemical Compositions ◽  
Strong Activity ◽  
Chemical Variability ◽  
Main Component

The chemical composition of essential oils extracted from aerial parts of Eryngium campestre collected in 37 localities from Western Algeria was characterized using GC-FID and GC/MS analyses. Altogether, 52 components, which accounted for 70.1 to 86.8% of the total composition oils were identified. The main compounds were Germacrene D (0.4–53.4%), Campestrolide (1.6–35.3%), Germacrene B (0.2–21.5%), Myrcene (0.1–8.4%), α-Cadinol (0.2–7.6%), Spathulenol (0.1–7.6%), Eudesma-4(15)-7-dien-1-β-ol (0.1–7.6%) and τ-Cadinol (0.3–5.5%). The chemical compositions of essential oils obtained from separate organs and during the complete vegetative cycle of the plant were also studied. With the uncommon 17-membered ring lactone named Campestrolide as the main component, Algerian E. campestre essential oils exhibited a remarkable chemical composition. A study of the chemical variability using statistical analysis allowed the discrimination of two main clusters according to the geographical position of samples. The study contributes to the better understanding of the relationship between the plant and its environment. Moreover, the antimicrobial activity of the essential oil was assessed against twelve strains bacteria and two yeasts involved in foodborne and nosocomial infections using paper disc diffusion and dilution agar assays. The in vitro study demonstrated a strong activity against Gram-positive strains such as S. aureus, B. cereus, and E. faecalis. The cytotoxicity and antiparasitic activities (on Lmm and Tbb) of the collective essential oil and one sample rich in campestrolide, as well as some enriched fractions or fractions containing other terpenic compounds, were also analyzed. Campestrolide seems to be one compound responsible for the cytotoxic and antileishmanial effect, while myrcene or/and trans-β-farnesene have a more selective antitrypanosomal activity.

scholarly journals Chemical composition, antimicrobial activity and chromosome number of Hertia cheirifolia L. from Algeria

2020 ◽  
Vol 7 (2) ◽  
pp. 31-43
Author(s):  
Ounoughi Abdelkader ◽  
Ramdani Messaoud ◽  
Lograda Takia ◽  
Chalard Pierre ◽  
Figueredo Gilles
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Chromosome Number ◽  
Essential Oil ◽  
Essential Oils ◽  
Diffusion Method ◽  
Chemical Compositions ◽  
Root Cells ◽  
Karyotypic Analysis ◽  
Germacrene D

AbstractThe aims of this work are to investigate the chemical composition, the antibacterial activity of the essential oil and the chromosome numbers of two populations of Hertia cheirifolia. The samples were collected in the flowering stage, in eastern Algeria locality. The aerial parts of H. cheirifolia were submitted to a hydro-distillation. GC and GC / MS analysed the chemical compositions of the obtained essential oils. The antibacterial activity of essential oils was evaluated using the disks diffusion method against ten bacterial strains. For karyotypic analysis, the squashing method is used. Fifty-eight compounds representing 98.93% of the total oil were identified in H. cheirifolia. The chemical composition is dominated by the presence of major products, α-pinene (48.49 - 53.85%) and Germacrene-D (2.64 - 12.66%). Two distinct chemical breeds were identified, the α-pinene-spathulenol of Batna population, and the α-pinene-germacrene-D of Setif population. The essential oil of H. cheirifolia has a moderate activity against bacteria tested. In contrast, the strains E. coli ATCC 25922, P. syringae ATCC 53543 and E. fecalis ATCC 49452 are resistant to H. cheirifolia essential oils. The observations of root cells meristematic at metaphase of H. cheirifolia gave a diploid chromosome number 2n = 2x = 20, with a basic chromosome number (x= 10).

scholarly journals Chemical Composition of Hypericum richeri subsp. grisebachii Essential Oil from Croatia

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800
Author(s):  
Igor Jerković ◽  
Maja Marasović ◽  
Zvonimir Marijanović ◽  
Kroata Hazler Pilepić ◽  
Željan Maleš ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Decanoic Acid ◽  
Chemical Profile ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Germacrene D

The aerial parts of Hypericum richeri Vill. subsp. grisebachii (Boiss.) Nyman were collected from two different locations in Croatia and subjected to hydrodistillation. GC/FID and GC/MS analysis of the isolated essential oils revealed 64 compounds representing 94.7% and 98.2% of the total oils. Predominant constituents in both samples were: germacrene D (10.9%; 6.0%), bicyclogermacrene (4.7%; 3.5%), α-pinene (6.8%; 6.9%), β-pinene (8.1%; 5.1%), decanoic acid (4.5%; 6.8%), β-caryophyllene (3.3%; 7.5%), δ-cadinene (7.0%; 4.4%), spathulenol (6.0%; 9.5%) and tetracosane (3.1%; 5.8%). Comparison of both samples revealed similarity in the chemical composition with minor fluctuations of constituent percentages. The chemical profile of Croatian oils was in general similar to those reported for other geographic areas regarding major mono- and sesquiterpene constituents. However, spathulenol, 5-cadinene and bicyclogermacrene were more abundant in Croatian oils. The presence of decanoic acid (4.5%; 6.8%) in Croatian oils was the major difference between acids and fatty acids derivatives. Higher abundance of alkanes (particularly tetracosane and docosane) was also noticed.

scholarly journals Essential Oil Profile of Vietnamese Callicarpa candicans (Burm. f.) Hochr

2020 ◽  
Vol 32 (7) ◽  
pp. 1630-1634
Author(s):  
Vu Thi Thu Le Vu Thi Thu Le ◽  
Lai Phuong Phuong Thao ◽  
Pham Thi Hong Minh ◽  
Hoang Thi Bich ◽  
Do Tien Lam ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Retention Indices ◽  
Chemical Compositions ◽  
Oil Performance

In this study, the essential oil profile of Callicarpa candicans (Burm. f.) Hochr was presented. Samples were collected from Vietnam and the oils were extracted from fresh leaves and dry leaves by hydrodistillation method. GC-MS data and retention indices were used to identify the chemical composition. The essential oil performance achieved 0.263% for fresh leaves and 1.503% for dry leaves. A total of 47 components were identified from fresh leaves oil and 39 constituents from dry leaves oil. Chemical compositions of the two essential oils were similar, with the major components being α-gurjunene, δ-cadinene, ε-caryophyllene (β-caryophyllene) and α-selinene.

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