Trace Elements at Whole Blood of Distinct Mouse Lines by Using NAA

2007 ◽  
Author(s):  
C. B. Zamboni ◽  
G. S. Zahn ◽  
O. A. Sant’Anna
Keyword(s):  
Trace Elements ◽  
Whole Blood ◽  
Mouse Lines

Biomonitoring of 29 trace elements in whole blood from inhabitants of Cotonou (Benin) by ICP-MS

2017 ◽  
Vol 43 ◽  
pp. 38-45 ◽  
Author(s):  
Brice Yedomon ◽  
Alain Menudier ◽  
Florence Lecavelier Des Etangs ◽  
Ludovic Anani ◽  
Benjamin Fayomi ◽  
...  
Keyword(s):  
Trace Elements ◽  
Whole Blood ◽  
Icp Ms

scholarly journals CHANGES OF NONESSENTIAL TRACE ELEMENTS LEVELS IN WHOLE BLOOD OF END STAGE RENAL DISEASE ADULT PATIENTS

2017 ◽  
pp. 114-125
Author(s):  
O. O. Makarov ◽  
E. O. Pisarev ◽  
B. S. Sheiman ◽  
M. V. Kulizkiy ◽  
D. V. Peretyatko ◽  
...  
Keyword(s):  
Trace Elements ◽  
Whole Blood ◽  
Chronic Kidney Disease Stage ◽  
Disease Stage ◽  
Blood Levels ◽  
Healthy Donors ◽  
Plasma Mass Spectrometry ◽  
Stage Renal Disease ◽  
End Stage ◽  
Esrd Patients

Aims: Accumulation of trace elements occurs in conditions of decreased or termination of kidneys functions. In some conditions increased trace elements can obtain toxic features. On other hand, researches are showing that concentration of some trace elements could be decreased in ERSD patients, too. The most important factor affecting trace element concentration in ERSD patient is the degree of renal failure and using of replacement therapy. Materials and methods: We determined the concentration of microelements (beryllium, boron, aluminum, vanadium, chromium, nickel, arsenic, rubidium, strontium, cadmium, cesium, barium, thallium and lead) in whole blood of 41 ESRD patients with chronic kidney disease stage VD, who were treated with hemodialysis / hemodiafiltration and in 61 conditionally healthy donors. Determination of whole bloods trace elements content was conducted using inductive coupled plasma mass- spectrometry (ICPMS).  Results: It is determinate that levels of beryllium, boron, aluminum, vanadium, chromium, strontium, cadmium, barium, thallium and lead is reliable increased in ESRD patients. Decreased levels are observed for nickel, arsenic, cesium and rubidium. Conclusions: ESRD is accompanied with substantial and multidirectional changes of trace elements blood levels. During researches has shown that in ESRD patients processes of trace elements accumulation are prevailing over elimination ones.

scholarly journals Trace Element Analysis of Cancerous and Non-cancerous Breast Tissues of African Women in Southwest Nigeria Using Particle-Induced X-ray Emission Technique

2019 ◽  
Vol 13 ◽  
pp. 117822341984069
Author(s):  
David O Olaiya ◽  
Olusegun I Alatise ◽  
Oyebamiji O Oketayo ◽  
Olawale E Abiye ◽  
Eusebius I Obianjunwa ◽  
...  
Keyword(s):  
Trace Elements ◽  
Whole Blood ◽  
Trace Element Analysis ◽  
African Women ◽  
Animal Blood ◽  
X Ray ◽  
Freeze Dried ◽  
Proton Beam Energy ◽  
Southwest Nigeria ◽  
Breast Tissues

In this study, we applied particle-induced X-ray emission (PIXE) spectroscopy to investigate the levels of trace elements in breast tissues and whole blood (cancerous and non-cancerous) of selected African women in Ile-Ife, Southwest Nigeria. Freeze-dried and homogenized specimens obtained through mastectomy from clinically diagnosed patients were made into 11-mm-diameter pellets. The pellets were irradiated with 2.5 MeV proton beam energy from a 1.7 MV 5SDH Tandem accelerator. The PIXE analytical system was calibrated with certified reference matrices of Bovine Liver and Animal Blood: NIST 1577a and IAEA-A-13, respectively. A total of 23 elements: Na, K, Ca, Cl, S, Al, P, Si, Zn, Pb, Br, Rb, Zr, Se, Sr, Mn, V, Ti, Cu, Fe, Ni, Cr, and Mg were detected. The results indicated that the levels were within 0.9-5288 and 0.6-2320 ppm in breast tissues and 0.3-17228 and 2.0-2475 ppm in the whole blood of cancerous and non-cancerous subjects, respectively. At the .05 level of significance, significant differences exist between these levels in the cancerous and non-cancerous breast tissues ( t = 0.008) as well as the whole blood ( t = 0.041). The results gave the baseline concentration of the observed trace elements in the normal and malignant subjects and indicated PIXE as a powerful tool for such investigation.

Trace Element Status (Zinc, Copper, Selenium, Iron, Manganese) in Patients with Long-Term Home Parenteral Nutrition

2016 ◽  
Vol 69 (2) ◽  
pp. 120-124 ◽  
Author(s):  
Milan Dastych Jr. ◽  
Michal Šenkyřík ◽  
Milan Dastych ◽  
František Novák ◽  
Petr Wohl ◽  
...  
Keyword(s):  
Trace Elements ◽  
Parenteral Nutrition ◽  
Blood Plasma ◽  
Whole Blood ◽  
Statistical Significance ◽  
Home Parenteral Nutrition ◽  
Control Group ◽  
The Status ◽  
In Blood Plasma

Background: The objective of the present study was to determine concentrations of zinc (Zn), copper (Cu), iron (Fe), selenium (Se) in blood plasma and manganese (Mn) in the whole blood in patients with long-term home parenteral nutrition (HPN) in comparison to the control group. Patients and Methods: We examined 68 patients (16 men and 52 women) aged from 28 to 68 years on a long-term HPN lasting from 4 to 96 months. The short bowel syndrome was an indication for HPN. The daily doses of Zn, Cu, Fe, Se and Mn in the last 3 months were determined. Results: No significant differences in blood plasma were found for Zn, Cu and Fe in patients with HPN and in the control group (p > 0.05). The concentration of Mn in whole blood was significantly increased in HPN patients (p < 0.0001), while Se concentration in these patients was significantly decreased (p < 0.005). The concentration of Mn in the whole blood of 16 patients with cholestasis was significantly increased compared to the patients without cholestasis (p < 0.001). The Cu concentration was increased with no statistical significance. Conclusion: In long-term HPN, the status of trace elements in the patients has to be continually monitored and the daily substitution doses of these elements have to be flexibly adjusted. Dosing schedule needs to be adjusted especially in cases of cholestatic hepatopathy. A discussion about the optimal daily dose of Mn in patients on HPN is appropriate. For clinical practice, the availability of a substitution mixture of trace elements lacking Mn would be advantageous.

Development and Validation of an ICP-MS Method and Its Application to Determine Multiple Trace Elements in Small Volumes of Whole Blood and Plasma

2020 ◽  
Author(s):  
E M Tanvir ◽  
Karen M Whitfield ◽  
Jack C Ng ◽  
P Nicholas Shaw
Keyword(s):  
Trace Elements ◽  
Nutritional Status ◽  
Trace Element ◽  
Human Blood ◽  
Whole Blood ◽  
Plasma Concentrations ◽  
Essential Elements ◽  
Small Sample ◽  
Plasma Samples ◽  
Element Concentrations

Abstract Essential and nonessential element concentrations in human blood provide important information on the nutritional status of individuals and can assist in the screening or diagnosis of certain disorders and their association with other causative factors. A simple and sensitive method, suitable for use with small sample volumes, for quantification of multiple trace element concentrations in whole blood and plasma has been developed using inductively coupled plasma-mass spectrometry. Method validation was performed using standard reference materials of whole blood and serum using varying sample treatments with nitric acid, water and hydrogen peroxide. The method was applied to quantify the trace element concentrations in whole blood and plasma samples (0.1 mL) from 50 adult blood donors in Queensland. The whole blood sample (5 mL) was collected in Vacutainer tubes with K2EDTA as anticoagulant. The developed method was able to quantify, in blood and plasma samples over a wide range of concentrations, several essential elements: cobalt, copper, zinc, iron, manganese and selenium; the nutritionally probably essential elements vanadium and strontium; and nonessential elements including lead, cadmium, arsenic, caesium, barium, thallium and uranium. Significant differences (P &lt; 0.0001) were observed between whole blood and plasma concentrations for 13 elements; 5 of the measured elements, cobalt (0.49 vs. 0.36 μg/L), copper (1.0 vs. 0.75 mg/L), strontium (28 vs. 16 μg/L), barium (1.5 vs. 0.64 μg/L) and thallium (0.06 vs. 0.03 μg/L), had higher mean concentrations in plasma than in blood. Whole blood concentrations of nine trace elements were significantly correlated (P &lt; 0.0001) with plasma concentrations. The distribution of the trace elements between human blood and plasma varied considerably for the different elements. These results indicate that, using a small sample volume, this assay is suitable for the evaluation of nutritional status as well as in monitoring human toxic elemental exposures.

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